Biotin

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Biotin
Biotin structure.svg
Biotin-view-1-from-xtal-Mercury-3D-balls.png
Names
Preferred IUPAC name
5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid
Other names
Vitamin B7
Identifiers
3D model (JSmol)
ChEBI
ChEMBL
ChemSpider
DrugBank
ECHA InfoCard 100.000.363 OOjs UI icon edit-ltr-progressive.svg
KEGG
PubChem CID
UNII
  • InChI=1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1 Yes check.svgY
    Key: YBJHBAHKTGYVGT-ZKWXMUAHSA-N Yes check.svgY
  • InChI=1/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
    Key: YBJHBAHKTGYVGT-ZKWXMUAHBB
  • O=C1N[C@@H]2[C@@H](SC[C@@H]2N1)CCCCC(=O)O
  • C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)O)NC(=O)N2
Properties
C10H16N2O3S
Molar mass 244.31 g·mol−1
AppearanceWhite crystalline needles
Melting point 232 to 233 °C (450 to 451 °F; 505 to 506 K)
22 mg/100 mL
Pharmacology
A11HA05 ( WHO )
Hazards
NFPA 704 (fire diamond)
NFPA 704.svgHealth 1: Exposure would cause irritation but only minor residual injury. E.g. turpentineFlammability 1: Must be pre-heated before ignition can occur. Flash point over 93 °C (200 °F). E.g. canola oilInstability 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogenSpecial hazards (white): no code
1
1
0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Yes check.svgY  verify  (what is  Yes check.svgYX mark.svgN ?)

Biotin (also known as vitamin B7 or vitamin H) is one of the B vitamins. [1] [2] [3] It is involved in a wide range of metabolic processes, both in humans and in other organisms, primarily related to the utilization of fats, carbohydrates, and amino acids. [4] The name biotin, borrowed from the German Biotin, derives from the Ancient Greek word βίοτος (bíotos; 'life') and the suffix "-in" (a suffix used in chemistry usually to indicate 'forming'). [5] Biotin appears as a white, needle-like crystalline solid. [6]

Chemical description

Biotin is classified as a heterocyclic compound, with a sulfur-containing tetrahydrothiophene ring fused to a ureido group. A C5-carboxylic acid side chain is appended to the former ring. The ureido ring, containing the −N−CO−N− group, serves as the carbon dioxide carrier in carboxylation reactions. [7] Biotin is a coenzyme for five carboxylase enzymes, which are involved in the catabolism of amino acids and fatty acids, synthesis of fatty acids, and gluconeogenesis. [3] [4] Biotinylation of histone proteins in nuclear chromatin plays a role in chromatin stability and gene expression. [4] [8]

Dietary recommendations

The US National Academy of Medicine updated Dietary Reference Intakes for many vitamins in 1998. At that time there was insufficient information to establish estimated average requirement or recommended dietary allowance, terms that exist for most vitamins. In instances such as this, the academy sets adequate intakes (AIs) with the understanding that at some later date, when the physiological effects of biotin are better understood, AIs will be replaced by more exact information. The biotin AIs for both males and females are:

Biotin Adequate Intakes (AIs) [2]
Age GroupBiotin AI (μg/day)
0 to 6 months5
7 to 12 months6
1 to 3 years8
4 to 8 years12
9 to 13 years20
14 to 18 years25
19 years and older30
Pregnant females (14 to 50 years)30
Lactating females (14 to 50 years)35

Australia and New Zealand set AIs similar to the US. [9]

The European Food Safety Authority (EFSA) also identifies AIs, setting values at 40 μg/day for adults, pregnancy at 40 μg/day, and breastfeeding at 45 μg/day. For children ages 1–17 years, the AIs increase with age from 20 to 35 μg/day. [10]

Safety

The US National Academy of Medicine estimates upper limits for vitamins and minerals when evidence for a true limit is sufficient. For biotin, however, there is no upper limit because adverse effects of high biotin intake have not been determined. [2] The EFSA also reviewed safety and reached the same conclusion as in the United States. [11]

Labeling regulations

For US food and dietary supplement labeling purposes, the amount in a serving is expressed as a percent of daily value. For biotin labeling purposes, 100% of the daily value was 300 μg/day, but as of May 27, 2016, it was revised to 30 μg/day to bring it into an agreement with the adequate intake. [12] [13] Compliance with the updated labeling regulations was required by January 1, 2020, for manufacturers with US$10 million or more in annual food sales, and by January 1, 2021, for manufacturers with lower volume food sales. [14] [15] A table of the old and new adult daily values is provided at Reference Daily Intake.

Sources

Source [16] Amount
(μg / 100 g)
Chicken liver187
Beef liver42
Eggs 21
Egg white5.8
Egg yolk27
Salmon, canned in water5.9
Pork chop4.5
Turkey breast0.7
Tuna, white, canned0.7
Source [16] Amount
(μg / 100 g)
Peanuts, roasted17.5
Sunflower seeds, roasted7.8
Almonds, roasted4.4
Sweet potato 1.5
Broccoli 0.9
Tomato 0.7
Strawberry 1.5
Avocado 1.0
Corn, canned0.05
Source [16] Amount
(μg / 100 g)
Cheese 1.4
Milk 0.1
Oatmeal 0.1
Bread 0.1
French fries 0.3
Wine 0.1
Beer 0.1
Potatoes, mashed0.1

Biotin is stable at room temperature and is not destroyed by cooking. The dietary biotin intake in Western populations has been estimated to be in the range of 35 to 70 μg/day. Nursing infants ingest about 6 μg/day. [4] Biotin is available in dietary supplements, individually or as an ingredient in multivitamins. [1] [3]

According to the Global Fortification Data Exchange, biotin deficiency is so rare that no countries require that foods be fortified. [17]

Physiology

Biotin is a water-soluble B vitamin. Consumption of large amounts as a dietary supplement results in absorption, followed by excretion into urine as biotin. Consumption of biotin as part of a normal diet results in urinary excretion of biotin and biotin metabolites. [4]

Absorption

Biotin in food is bound to proteins. Digestive enzymes reduce the proteins to biotin-bound peptides. The intestinal enzyme biotinidase, found in pancreatic secretions and in the brush border membranes of all three parts of the small intestine, frees biotin, which is then absorbed from the small intestine. [4] When consumed as a biotin dietary supplement, absorption is nonsaturable, meaning that even very high amounts are absorbed effectively. Transport across the jejunum is faster than across the ileum. [4]

The large intestine microbiota synthesize amounts of biotin estimated to be similar to the amount taken in the diet, and a significant portion of this biotin exists in the free (protein-unbound) form and, thus, is available for absorption. How much is absorbed in humans is unknown, although a review did report that human epithelial cells of the colon in vitro demonstrated an ability to uptake biotin. [18]

Once absorbed, sodium-dependent multivitamin transporter (SMVT) mediates biotin uptake into the liver. [4] SMVT also binds pantothenic acid, so high intakes of either of these vitamins can interfere with transport of the other. [19]

Metabolism and excretion

Biotin catabolism occurs via two pathways. In one, the valeric acid sidechain is cleaved, resulting in bisnorbiotin. In the other pathway, the sulfur is oxidized, resulting in biotin sulfoxide. Urine content is proportionally about half biotin, plus bisnorbiotin, biotin sulfoxide, and small amounts of other metabolites. [4]

Factors that affect biotin requirements

Chronic alcohol use is associated with a significant reduction in plasma biotin. [20] Intestinal biotin uptake also appears to be sensitive to the effect of the anti-epilepsy drugs carbamazepine and primidone. [20] Relatively low levels of biotin have also been reported in the urine or plasma of patients who have had a partial gastrectomy or have other causes of achlorhydria, as well as burn patients, elderly individuals, and athletes. [21] Pregnancy and lactation may be associated with an increased demand for biotin. In pregnancy, this may be due to a possible acceleration of biotin catabolism, whereas, in lactation, the higher demand has yet to be elucidated. Recent studies have shown marginal biotin deficiency can be present in human gestation, as evidenced by increased urinary excretion of 3-hydroxyisovaleric acid, decreased urinary excretion of biotin and bisnorbiotin, and decreased plasma concentration of biotin. [4]

Biosynthesis

Biotin, synthesized in plants, is essential to plant growth and development. [22] Bacteria also synthesize biotin, [23] and it is thought that bacteria resident in the large intestine may synthesize biotin that is absorbed and utilized by the host organism. [18]

Biosynthesis starts from two precursors, alanine and pimeloyl-CoA. These form 7-keto-8-aminopelargonic acid (KAPA). KAPA is transported from plant peroxisomes to mitochondria where it is converted to 7,8-diaminopelargonic acid (DAPA) with the help of the enzyme, BioA. The enzyme dethiobiotin synthetase catalyzes the formation of the ureido ring via a DAPA carbamate activated with ATP, creating dethiobiotin with the help of the enzyme, BioD, which is then converted into biotin which is catalyzed by BioB. [24] The last step is catalyzed by biotin synthase, a radical SAM enzyme. The sulfur is donated by an unusual [2Fe-2S] ferredoxin. [25] Depending on the species of bacteria, Biotin can be synthesized via multiple pathways. [24]

Cofactor biochemistry

The enzyme holocarboxylase synthetase covalently attaches biotin to five human carboxylase enzymes: [4]

For the first two, biotin serves as a cofactor responsible for transfer of bicarbonate to acetyl-CoA, converting it to malonyl-CoA for fatty acid synthesis. PC participates in gluconeogenesis. MCC catalyzes a step in leucine metabolism. PCC catalyzes a step in the metabolism of propionyl-CoA. [1] [3] [4] Metabolic degradation of the biotinylated carboxylases leads to the formation of biocytin. This compound is further degraded by biotinidase to release biotin, which is then reutilized by holocarboxylase synthetase. [4]

Biotinylation of histone proteins in nuclear chromatin is a posttranslational modification that plays a role in chromatin stability and gene expression. [4] [8]

Deficiency

Primary biotin deficiency, meaning deficiency as a consequence of too little biotin in the diet, is rare, because biotin is contained in so many foods. Subclinical deficiency can cause mild symptoms, such as hair thinning, brittle fingernails, or skin rash, typically on the face. [2] [4]

Aside from inadequate dietary intake (rare), deficiency of biotin can be caused by a genetic disorder that affects biotin metabolism. The most common among these is biotinidase deficiency. Low activity of this enzyme causes a failure to recycle biotin from biocytin. Rarer are carboxylase and biotin transporter deficiences. [4] [26] Neonatal screening for biotinidase deficiency started in the United States in 1984, with many countries now also testing for this genetic disorder at birth. Treatment is lifelong dietary supplement with biotin. [1] If biotinidase deficiency goes untreated, it can be fatal. [27]

Diagnosis

Low serum and urine biotin are not sensitive indicators of inadequate biotin intake. [4] However, serum testing can be useful for confirmation of consumption of biotin-containing dietary supplements, and whether a period of refraining from supplement use is long enough to eliminate the potential for interfering with drug tests. [28] [29] Indirect measures depend on the biotin requirement for carboxylases. 3-Methylcrotonyl-CoA is an intermediate step in the catabolism of the amino acid leucine. In the absence of biotin, the pathway diverts to 3-hydroxyisovaleric acid. Urinary excretion of this compound is an early and sensitive indicator of biotin deficiency. [2] [4]

Deficiency as a result of metabolic disorders

Biotinidase deficiency is a deficiency of the enzyme that recycles biotin, the consequence of an inherited genetic mutation. [1] Biotinidase catalyzes the cleavage of biotin from biocytin and biotinyl-peptides (the proteolytic degradation products of each holocarboxylase) and thereby recycles biotin. [2] It is also important in freeing biotin from dietary protein-bound biotin. [30] Neonatal screening for biotinidase deficiency started in the United States in 1984, [31] which as of 2017 was reported as required in more than 30 countries. [32]

Profound biotinidase deficiency, defined as less than 10% of normal serum enzyme activity, which has been reported as 7.1 nmol/min/mL, has an incidence of 1 in 40,000 to 1 in 60,000, but with rates as high as 1 in 10,000 in countries with high incidence of consanguineous marriages (second cousin or closer). Partial biotinidase deficiency is defined as 10% to 30% of normal serum activity. [31] Incidence data stems from government mandated newborn screening. [32] For profound deficiency, treatment is oral dosing with 5 to 20 mg per day. Seizures are reported as resolving in hours to days, with other symptoms resolving within weeks. [31] Treatment of partial biotinidase deficiency is also recommended even though some untreated people never manifest symptoms. [31] Lifelong treatment with supplemental biotin is recommended for both profound and partial biotinidase deficiency. [1]

Inherited metabolic disorders characterized by deficient activities of biotin-dependent carboxylases are termed multiple carboxylase deficiency. These include deficiencies in the enzymes holocarboxylase synthetase. [1] Holocarboxylase synthetase deficiency prevents the body's cells from using biotin effectively and thus interferes with multiple carboxylase reactions. [30] There can also be a genetic defect affecting the sodium-dependent multivitamin transporter protein. [26]

Biochemical and clinical manifestations of any of these metabolic disorders can include ketolactic acidosis, organic aciduria, hyperammonemia, rash, hypotonia, seizures, developmental delay, alopecia and coma. [4]

Use in biotechnology

Chemically modified versions of biotin are widely used throughout the biotechnology industry to isolate proteins and non-protein compounds for biochemical assays. [33] Because egg-derived avidin binds strongly to biotin with a dissociation constant Kd ≈ 10−15 M, [34] biotinylated compounds of interest can be isolated from a sample by exploiting this highly stable interaction. First, the chemically modified biotin reagents are bound to the targeted compounds in a solution via a process called biotinylation. The choice of which chemical modification to use is responsible for the biotin reagent binding to a specific protein. [33] Second, the sample is incubated with avidin bound to beads, then rinsed, removing all unbound proteins, while leaving only the biotinylated protein bound to avidin. Last, the biotinylated protein can be eluted from the beads with excess free biotin. [35] The process can also utilize bacteria-derived streptavidin bound to beads, but because it has a higher dissociation constant than avidin, very harsh conditions are needed to elute the biotinylated protein from the beads, which often will denature the protein of interest. [34]

Interference with medical laboratory results

When people are ingesting high levels of biotin in dietary supplements, a consequence can be clinically significant interference with diagnostic blood tests that use biotin-streptavidin technology. This methodology is commonly used to measure levels of hormones such as thyroid hormones, and other analytes such as 25-hydroxyvitamin D. Biotin interference can produce both falsely normal and falsely abnormal results. [1] [36] In the US, biotin as a non-prescription dietary supplement is sold in amounts of 1 to 10 mg per serving, with claims for supporting hair and nail health, and as 300 mg per day as a possibly effective treatment for multiple sclerosis [37] [38] (see § Research). Overconsumption of 5 mg/day or higher causes elevated concentration in plasma that interferes with biotin-streptavidin immunoassays in an unpredictable manner. [28] [29] Healthcare professionals are advised to instruct patients to stop taking biotin supplements for 48 h or even up to weeks before the test, depending on the specific test, dose, and frequency of biotin uptake. [28] Guidance for laboratory staff is proposed to detect and manage biotin interference. [29]

History

In 1916, W. G. Bateman observed that a diet high in raw egg whites caused toxic symptoms in dogs, cats, rabbits, and humans. [39] By 1927, scientists such as Margarete Boas and Helen Parsons had performed experiments demonstrating the symptoms associated with "egg-white injury." They had found that rats fed large amounts of egg-white as their only protein source exhibited neurological dysfunction, hair loss, dermatitis, and eventually, death. [40] [41]

In 1936, Fritz Kögl and Benno Tönnis documented isolating a yeast growth factor in a journal article titled "Darstellung von krystallisiertem biotin aus eigelb." (Representation of crystallized biotin from egg yolk). [42] The name biotin derives from the Greek word bios ('to live') and the suffix "-in" (a general chemical suffix used in organic chemistry). [5] Other research groups, working independently, had isolated the same compound under different names. Hungarian scientist Paul Gyorgy began investigating the factor responsible for egg-white injury in 1933 and in 1939, was successful identifying what he called "Vitamin H" (the H represents Haar und Haut, German for 'hair and skin'). [43] [44] Further chemical characterization of vitamin H revealed that it was water-soluble and present in high amounts in the liver. [45] After experiments performed with yeast and Rhizobium trifolii, West and Wilson isolated a compound they called co-enzyme R. [46] [47] By 1940, it was recognized that all three compounds were identical and were collectively given the name: biotin. [48] Gyorgy continued his work on biotin and in 1941 published a paper demonstrating that egg-white injury was caused by the binding of biotin by avidin. [49] [50] Unlike for many vitamins, there is insufficient information to establish a recommended dietary allowance, so dietary guidelines identify an "adequate intake" based on best available science with the understanding that at some later date this will be replaced by more exact information. [2] [9] [10]

Using E. coli, a biosynthesis pathway was proposed by Rolfe and Eisenberg in 1968. The initial step was described as a condensation of pimelyl-CoA and alanine to form 7-oxo-8-aminopelargonic acid. From there, they described three-step process, the last being introducing a sulfur atom to form the tetrahydrothiophene ring. [51]

Research

Multiple sclerosis

High-dose biotin (300 mg/day = 10,000 times adequate intake) has been used in clinical trials for treatment of multiple sclerosis, a demyelinating autoimmune disease. [37] [38] The hypothesis is that biotin may promote remyelination of the myelin sheath of nerve cells, slowing or even reversing neurodegeneration. The proposed mechanisms are that biotin activates acetyl-coA carboxylase, which is a key rate-limiting enzyme during the synthesis of myelin, and by reducing axonal hypoxia through enhanced energy production. [37] [38] Clinical trial results are mixed; a 2019 review concluded that a further investigation of the association between multiple sclerosis symptoms and biotin should be undertaken, [37] whereas two 2020 reviews of a larger number of clinical trials reported no consistent evidence for benefits, [52] and some evidence for increased disease activity and higher risk of relapse. [53]

Hair, nails, skin

In the United States, biotin is promoted as a dietary supplement for strengthening hair and fingernails, though scientific data supporting these outcomes in humans are very weak. [3] [54] [55] A review of the fingernails literature reported brittle nail improvement as evidence from two pre-1990 clinical trials that had administered an oral dietary supplement of 2.5 mg/day for several months, without a placebo control comparison group. There is no more recent clinical trial literature. [54] A review of biotin as treatment for hair loss identified case studies of infants and young children with genetic defect biotin deficiency having improved hair growth after supplementation, but went on to report that "there have been no randomized, controlled trials to prove efficacy of supplementation with biotin in normal, healthy individuals." [55] Biotin is also incorporated into topical hair and skin products with similar claims. [56]

The Dietary Supplement Health and Education Act of 1994 states that the US Food and Drug Administration must allow on the product label what are described as "Structure:Function" (S:F) health claims that ingredient(s) are essential for health. For example: Biotin helps maintain healthy skin, hair and nails. If a S:F claim is made, the label must include the disclaimer "This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease." [57]

Animals

In cattle, biotin is necessary for hoof health. Lameness due to hoof problems is common, with herd prevalence estimated at 10 to 35%. [58] Consequences of lameness include less food consumption, lower milk production, and increased veterinary treatment costs. Results after 4–6 months from supplementing biotin at 20 mg/day into daily diet reduces the risk of lameness. [58] [59] A review of controlled trials reported that supplementation at 20 mg/day increased milk yield by 4.8%. The discussion speculated that this could be an indirect consequence of improved hoof health or a direct effect on milk production. [60]

For horses, conditions such as chronic laminitis, cracked hooves, or dry, brittle feet incapable of holding shoes are a common problem. Biotin is a popular nutritional supplement. There are recommendations that horses need 15 to 25 mg/day. Studies report biotin improves the growth of new hoof horn rather than improving the status of existing hoof, so months of supplementation are needed for the hoof wall to be completely replaced. [61]

See also

Related Research Articles

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References

  1. 1 2 3 4 5 6 7 8 "Biotin – Fact Sheet for Health Professionals". Office of Dietary Supplements, US National Institutes of Health. December 8, 2017. Archived from the original on April 14, 2020. Retrieved February 25, 2018.
  2. 1 2 3 4 5 6 7 Institute of Medicine (1998). "Biotin". Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline. Washington, DC: The National Academies Press. pp. 374–89. ISBN   0-309-06554-2. Archived from the original on July 17, 2015. Retrieved August 29, 2017.
  3. 1 2 3 4 5 "Biotin". Micronutrient Information Center, Linus Pauling Institute, Oregon State University, Corvallis, OR. October 21, 2015. Archived from the original on January 18, 2018. Retrieved January 16, 2018.
  4. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Penberthy WT, Sadri M, Zempleni J (2020). "Biotin". In BP Marriott, DF Birt, VA Stallings, AA Yates (eds.). Present Knowledge in Nutrition, Eleventh Edition. London, United Kingdom: Academic Press (Elsevier). pp. 289–304. ISBN   978-0-323-66162-1.
  5. 1 2 "biotin | Origin and meaning of biotin by Online Etymology Dictionary". www.etymonline.com. Archived from the original on August 22, 2020. Retrieved November 14, 2020.
  6. ↑ Anonymous PubChem Compound Summary for CID 171548, Biotin. https://pubchem.ncbi.nlm.nih.gov/compound/171548 Archived August 6, 2023, at the Wayback Machine (accessed Oct 19, 2023).
  7. ↑ Waldrop GL, Holden HM, St Maurice M (November 2012). "The Enzymes of Biotin dependent CO₂ Metabolism: What Structures Reveal about Their Reaction Mechanisms". Protein Science. 21 (11): 1597–1619. doi:10.1002/pro.2156. PMC   3527699 . PMID   22969052.
  8. 1 2 Xu YM, Du JY, Lau AT (September 2014). "Posttranslational modifications of human histone H3: an update". Proteomics. 14 (17–18): 2047–2060. doi:10.1002/pmic.201300435. PMID   25044606. S2CID   11293428.
  9. 1 2 "National Health and Medical Research Council: Nutrient Reference Values for Australia and New Zealand" (PDF). Archived from the original (PDF) on January 21, 2017. Retrieved February 19, 2010.
  10. 1 2 "Overview on Dietary Reference Values for the EU population as derived by the EFSA Panel on Dietetic Products, Nutrition and Allergies" (PDF). 2017. Archived (PDF) from the original on August 28, 2017. Retrieved August 30, 2017.
  11. ↑ "Tolerable Upper Intake Levels For Vitamins And Minerals" (PDF). European Food Safety Authority. 2006. Archived (PDF) from the original on March 16, 2016. Retrieved March 4, 2016.
  12. ↑ "Federal Register May 27, 2016 Food Labeling: Revision of the Nutrition and Supplement Facts Labels" (PDF). Archived (PDF) from the original on September 22, 2017.
  13. ↑ "Daily Value Reference of the Dietary Supplement Label Database (DSLD)". Dietary Supplement Label Database (DSLD). Archived from the original on April 7, 2020. Retrieved May 16, 2020.
  14. ↑ "Changes to the Nutrition Facts Label". U.S. Food and Drug Administration (FDA). May 27, 2016. Archived from the original on May 6, 2018. Retrieved May 16, 2020.PD-icon.svg This article incorporates text from this source, which is in the public domain .
  15. ↑ "Industry Resources on the Changes to the Nutrition Facts Label". U.S. Food and Drug Administration (FDA). December 21, 2018. Archived from the original on December 25, 2020. Retrieved May 16, 2020.PD-icon.svg This article incorporates text from this source, which is in the public domain .
  16. 1 2 3 Staggs CG, Sealey WM, McCabe BJ, Teague AM, Mock DM (December 2004). "Determination of the biotin content of select foods using accurate and sensitive HPLC/avidin binding". Journal of Food Composition and Analysis. 17 (6): 767–76. doi:10.1016/j.jfca.2003.09.015. PMC   1450323 . PMID   16648879.
  17. ↑ "Map: Count of Nutrients In Fortification Standards". Global Fortification Data Exchange. Archived from the original on April 11, 2019. Retrieved January 11, 2011.
  18. 1 2 Said HM (November 2013). "Recent advances in transport of water-soluble vitamins in organs of the digestive system: a focus on the colon and the pancreas". Am J Physiol Gastrointest Liver Physiol. 305 (9): G601–10. doi:10.1152/ajpgi.00231.2013. PMC   3840235 . PMID   23989008.
  19. ↑ Chirapu SR, Rotter CJ, Miller EL, Varma MV, Dow RL, Finn MG (March 31, 2013). "High specificity in response of the sodium-dependent multivitamin transporter to derivatives of pantothenic acid". Current Topics in Medicinal Chemistry. 13 (7): 837–42. doi:10.2174/1568026611313070006. PMID   23578027.
  20. 1 2 Said HM (August 2011). "Intestinal absorption of water-soluble vitamins in health and disease". Biochem J. 437 (3): 357–72. doi:10.1042/BJ20110326. PMC   4049159 . PMID   21749321.
  21. ↑ Combs GF (2008). The Vitamins: Fundamental Aspects in Nutrition and Health. San Diego: Elsevier, Inc. ISBN   978-0-12-183493-7.
  22. ↑ Maruyama J, Yamaoka S, Matsuo I, Tsutsumi N, Kitamoto K (December 2012). "A newly discovered function of peroxisomes: involvement in biotin biosynthesis". Plant Signal Behav. 7 (12): 1589–93. doi:10.4161/psb.22405. PMC   3578898 . PMID   23073000.
  23. ↑ Satiaputra J, Shearwin KE, Booker GW, Polyak SW (March 2016). "Mechanisms of biotin-regulated gene expression in microbes". Synth Syst Biotechnol. 1 (1): 17–24. doi:10.1016/j.synbio.2016.01.005. PMC   5640590 . PMID   29062923.
  24. 1 2 Hu Y, Cronan JE (November 5, 2020). "α-proteobacteria synthesize biotin precursor pimeloyl-ACP using BioZ 3-ketoacyl-ACP synthase and lysine catabolism". Nature Communications. 11 (1): 5598. Bibcode:2020NatCo..11.5598H. doi:10.1038/s41467-020-19251-5. ISSN   2041-1723. PMC   7645780 . PMID   33154364.
  25. ↑ Cramer JD, Jarrett JT (2018). "Purification, Characterization, and Biochemical Assays of Biotin Synthase from Escherichia coli". Radical SAM Enzymes. Methods in Enzymology. Vol. 606. pp. 363–388. doi:10.1016/bs.mie.2018.06.003. ISBN   9780128127940. PMID   30097099.
  26. 1 2 Zempleni J, Hassan YI, Wijeratne SS (November 2008). "Biotin and biotinidase deficiency". Expert Review of Endocrinology & Metabolism. 3 (6): 715–24. doi:10.1586/17446651.3.6.715. PMC   2726758 . PMID   19727438.
  27. ↑ Wolf B (September 2016). "Biotinidase deficiency and our champagne legacy". Gene. 589 (2): 142–50. doi:10.1016/j.gene.2015.10.010. PMID   26456103.
  28. 1 2 3 Luong JH, Vashist SK (January 2020). "Chemistry of Biotin-Streptavidin and the Growing Concern of an Emerging Biotin Interference in Clinical Immunoassays". ACS Omega. 5 (1): 10–18. doi:10.1021/acsomega.9b03013. PMC   6963918 . PMID   31956746.
  29. 1 2 3 Bowen R, Benavides R, Colón-Franco JM, Katzman BM, Muthukumar A, Sadrzadeh H, Straseski J, Klause U, Tran N (December 2019). "Best practices in mitigating the risk of biotin interference with laboratory testing". Clin Biochem. 74: 1–11. doi: 10.1016/j.clinbiochem.2019.08.012 . PMID   31473202.
  30. 1 2 Wolf B, Grier RE, Secor McVoy JR, Heard GS (1985). "Biotinidase deficiency: a novel vitamin recycling defect". Journal of Inherited Metabolic Disease. 8 (Suppl 1): 53–8. doi:10.1007/BF01800660. PMID   3930841. S2CID   11554577.
  31. 1 2 3 4 Canda E, Kalkan Uçar S, Çoker M (2020). "Biotinidase Deficiency: Prevalence, Impact And Management Strategies". Pediatric Health Med Ther. 11: 127–33. doi: 10.2147/PHMT.S198656 (inactive January 31, 2024). PMC   7211084 . PMID   32440248.{{cite journal}}: CS1 maint: DOI inactive as of January 2024 (link)
  32. 1 2 Glynis A (November 2012). "A Double-blind, Placebo-controlled Study Evaluating the Efficacy of an Oral Supplement in Women with Self-perceived Thinning Hair". The Journal of Clinical and Aesthetic Dermatology. 5 (11): 28–34. PMC   3509882 . PMID   23198010.
  33. 1 2 "Overview of Protein Labeling". Thermo Fisher Scientific. Archived from the original on September 26, 2012. Retrieved April 22, 2012.
  34. 1 2 Laitinen OH, Hytönen VP, Nordlund HR, Kulomaa MS (December 2006). "Genetically engineered avidins and streptavidins". Cellular and Molecular Life Sciences. 63 (24): 2992–3017. doi:10.1007/s00018-006-6288-z. PMC   11136427 . PMID   17086379. S2CID   7180383.
  35. ↑ Morag E, Bayer EA, Wilchek M (December 1996). "Immobilized nitro-avidin and nitro-streptavidin as reusable affinity matrices for application in avidin-biotin technology". Anal Biochem. 243 (2): 257–263. doi:10.1006/abio.1996.0514. PMID   8954558.
  36. ↑ "The FDA Warns that Biotin May Interfere with Lab Tests: FDA Safety Communication". US Food and Drug Administration. November 28, 2017. Archived from the original on April 24, 2019. Retrieved January 5, 2021.
  37. 1 2 3 4 Tryfonos C, Mantzorou M, Fotiou D, Vrizas M, Vadikolias K, Pavlidou E, Giaginis C (September 2019). "Dietary Supplements on Controlling Multiple Sclerosis Symptoms and Relapses: Current Clinical Evidence and Future Perspectives". Medicines. 6 (3): 95. doi: 10.3390/medicines6030095 . PMC   6789617 . PMID   31547410.
  38. 1 2 3 Sedel F, Bernard D, Mock DM, Tourbah A (November 2016). "Targeting demyelination and virtual hypoxia with high-dose biotin as a treatment for progressive multiple sclerosis". Neuropharmacology. 110 (Pt B): 644–53. doi: 10.1016/j.neuropharm.2015.08.028 . PMID   26327679.
  39. ↑ Bateman WG (June 1916). "The Digestibility and Utilization of Egg Proteins" (PDF). Journal of Biological Chemistry. 26: 263–91. doi: 10.1016/S0021-9258(18)87458-0 .
  40. ↑ Boas MA (1927). "The Effect of Desiccation upon the Nutritive Properties of Egg-white". The Biochemical Journal. 21 (3): 712–724.1. doi:10.1042/bj0210712. PMC   1251968 . PMID   16743887.
  41. ↑ Parsons HT, Kelly E (1933). "The Character of the Dermatitis-Producing Factor in Dietary Egg White as Shown by Certain Chemical Treatments". The Journal of Biological Chemistry. 100 (11): 377–379. doi:10.1111/j.1753-4887.1980.tb05948.x. PMID   7005763. S2CID   86107167.
  42. ↑ Kögl and Tönnis (1936). "Über das Bios-Problem. Darstellung von krystallisiertem Biotin aus Eigelb. 20. Mitteilung über pflanzliche Wachstumsstoffe". Hoppe-Seyler's Zeitschrift für Physiologische Chemie. 242 (1–2): 43–73. doi:10.1515/bchm2.1936.242.1-2.43.
  43. ↑ György P (December 1939). "The Curative Factor (vitamin H) for Egg White Injury, with Particular Reference to Its Presence in Different Foodstuffs and in Yeast". Journal of Biological Chemistry. 131 (2): 733–44. doi: 10.1016/S0021-9258(18)73468-6 . Archived from the original on January 13, 2021. Retrieved January 10, 2021.
  44. ↑ György P, Kuhn R, Lederer E (December 1939). "Attempts to Isolate the Factor (vitamin H) Curative of Egg White Injury". Journal of Biological Chemistry. 131 (2): 745–59. doi: 10.1016/S0021-9258(18)73469-8 . Archived from the original on January 14, 2021. Retrieved January 10, 2021.
  45. ↑ Birch TW, György P (December 1939). "Physicochemical Properties of the Factor (vitamin H) Curative of Egg White Injury". Journal of Biological Chemistry. 131 (2): 761–66. doi: 10.1016/S0021-9258(18)73470-4 . Archived from the original on January 14, 2021. Retrieved January 10, 2021.
  46. ↑ West PM, Wilson PW (June 1939). "The Relation of "coenzyme R" to Biotin". Science. 89 (2322): 607–8. Bibcode:1939Sci....89..607W. doi:10.1126/science.89.2322.607. PMID   17751623. S2CID   30138816.
  47. ↑ DuVigneaud V, Hofmann K, Melville DB, György P (August 1941). "Isolation of Biotin (vitamin H) from Liver". Journal of Biological Chemistry. 140 (2): 643–51. doi: 10.1016/S0021-9258(18)51355-7 . Archived from the original on January 13, 2021. Retrieved January 10, 2021.
  48. ↑ György P, Rose CS, Hofmann K, Melville DB, DU Vigneaud V (December 1940). "A Further Note on the Identity of Vitamin H with Biotin". Science. 92 (2400): 609. Bibcode:1940Sci....92..609G. doi:10.1126/science.92.2400.609. PMID   17795447.
  49. ↑ György P, Rose CS, Eakin RE, Snell EE, Williams RJ (May 1941). "Egg-White Injury as the Result of Nonabsorption or Inactivation of Biotin". Science. 93 (2420): 477–8. Bibcode:1941Sci....93..477G. doi:10.1126/science.93.2420.477. JSTOR   1668938. PMID   17757050.
  50. ↑ Gyorgy P, Rose CS (1943). "The Liberation of Biotin from the Avidin-Biotin Complex (AB)". Experimental Biology and Medicine. 53 (1): 55–7. doi:10.3181/00379727-53-14183. S2CID   84419614.
  51. ↑ Rolfe B, Eisenberg MA (August 1968). "Genetic and biochemical analysis of the biotin loci of Escherichia coli K-12". Journal of Bacteriology. 96 (2): 515–24. doi:10.1128/JB.96.2.515-524.1968. PMC   252325 . PMID   4877129.
  52. ↑ Motte J, Gold R (December 2020). "High-dose biotin in multiple sclerosis: the end of the road". Lancet Neurol. 19 (12): 965–66. doi:10.1016/S1474-4422(20)30353-7. PMID   33222766. S2CID   225049079.
  53. ↑ Goldschmidt CH, Cohen JA (July 2020). "The Rise and Fall of High-Dose Biotin to Treat Progressive Multiple Sclerosis". Neurotherapeutics. 17 (3): 968–70. doi: 10.1007/s13311-020-00907-5 . PMC   7609671 . PMID   32761325.
  54. 1 2 Cashman MW, Sloan SB (2010). "Nutrition and nail disease". Clin Dermatol. 28 (4): 420–5. doi:10.1016/j.clindermatol.2010.03.037. PMID   20620759.
  55. 1 2 Patel DP, Swink SM, Castelo-Soccio L (August 2017). "A Review of the Use of Biotin for Hair Loss". Skin Appendage Disord. 3 (3): 166–69. doi:10.1159/000462981. PMC   5582478 . PMID   28879195.
  56. ↑ Fiume MZ (2001). "Final report on the safety assessment of biotin". International Journal of Toxicology. 20 (Suppl 4): 1–12. doi:10.1080/10915810160233712. PMID   11800048.
  57. ↑ Janet Rehnquist (March 2003), Department of Health and Human Services – Office of the Inspector General – Dietary Supplement Labels: Key Elements (PDF), United States Department of Health and Human Services, OEI-01-01-00120, archived (PDF) from the original on November 18, 2004, retrieved April 2, 2013
  58. 1 2 Langova L, Novotna I, Nemcova P, Machacek M, Havlicek Z, Zemanova M, Chrast V (October 2020). "Impact of Nutrients on the Hoof Health in Cattle". Animals. 10 (10): 1824. doi: 10.3390/ani10101824 . PMC   7600182 . PMID   33036413.
  59. ↑ "Biotin and lameness - A review". Cattle Practice. 13 (Part 2): 145–53. October 2005. Archived from the original on May 17, 2022. Retrieved May 17, 2022.
  60. ↑ Chen B, Wang C, Wang YM, Liu JX (July 2011). "Effect of biotin on milk performance of dairy cattle: a meta-analysis". J Dairy Sci. 94 (7): 3537–46. doi: 10.3168/jds.2010-3764 . PMID   21700041.
  61. ↑ "Biotin Basics". Kentucky Equine Basics. November 4, 2003. Archived from the original on June 10, 2021. Retrieved January 18, 2021.
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