Search Results (136)

Background/Objectives: Early postmortem mitochondrial function and apoptotic activation affect meat quality development. Nicotinamide riboside (NR) supplementation to pigs prior to harvest can improve pork color stability, but its mechanism remains unclear. This study aimed to evaluate the impact of NR supplementation on early postmortem mitochondrial functionality and apoptosis. Methods: Sixteen pigs (N = 16) were individually fed a control or NR-supplemented diet (30 mg·kg body weight⁻¹·d⁻¹) for 10 days prior to harvest. Longissimus dorsi muscle samples were collected at 45 min and 24 h postmortem and analyzed for mitochondrial functionality using high-resolution respirometry and apoptotic protein abundance (apoptosis regulator Bcl-2-associated X (BAX), apoptotic inducing factor (AIF), and caspase 3 (CASP3)) via immunoblotting. Results: NR-supplemented muscle exhibited lower proton leak-associated respiration at 45 min postmortem (p < 0.05), followed by a slower accumulation of mitochondrial outer membrane permeabilization (MOMP; p < 0.05) and a slower loss of mitochondrial integral function (p < 0.05) from 45 min to 24 h postmortem. NR supplementation decreased BAX abundance at 45 min postmortem but increased mature AIF abundance (62 kDa) at 24 h postmortem (p < 0.05). The abundance of CASP3 fragments (~29 kDa) decreased from 45 min to 24 h postmortem, independent of treatment (p < 0.05). Conclusions: NR supplementation demonstrated the potential to protect mitochondrial integral function and alleviate apoptotic activation in early postmortem porcine skeletal muscle, which might contribute to a higher meat color stability in NR-supplemented pork during retail display. Full article

High-pressure treatment was utilized in this study to produce high-quality, reduced-sodium pork gels with desirable texture and sensory properties, addressing the challenge of maintaining quality in low-sodium meat products to meet health-conscious consumer demands. High-pressure treatment applied within the range of 150–200 MPa significantly reduced cooking loss while maintaining moisture content and provided an ideal network structure for reduced-sodium pork gels. High-pressure treatment at up to 100–200 MPa, in combination with added sodium chloride and sodium polyphosphate, was evaluated for its effects on gel texture, with results indicating that high-pressure treatment significantly improved breaking stress (increased by 10.01% under 150 MPa and 14.66% under 200 MPa), modulus of elasticity (increased by 14.77% under 150 MPa and 24.17% under 200 MPa), and hardness (increased by 11.12% under 150 MPa and 11.45% under 200 MPa). Rheological characteristic measurements revealed that gel strength was highest at 150 MPa (G′ = 443,000 Pa; G″ = 66,300 Pa and tanδ = 0.15), which showed higher G′ and G″ values and similar tanδ compared to the 0.1 MPa, 2% NaCl + 0.5% SPP condition (G′ = 334,000 Pa; G″ = 49,200 Pa; tanδ = 0.148). Protein analysis by sodium dodecyl sulfate–polyacrylamide gel electrophoresis showed a reduction in the α-actinin band with increased pressure, which suggested protein interactions were enhanced. Differential scanning calorimetry analysis indicated that protein denaturation occurred more readily at higher pressures (0.071 J/g at 0.1 MPa, 0.057 J/g at 150 MPa, and 0.039 J/g at 200 MPa). These findings underscore the value of treatment under high pressure at 150 MPa developing reduced-sodium meat products with desirable texture and flavor characteristics. Full article

The combined effects of pulsed light and a rotating magnetic field on the quality of raw pork loin stored under refrigerated conditions were studied. Muscles from the same carcass were divided into six distinct portions. Three portions were designated as untreated control samples, while the other three underwent experimental procedures involving exposure to pulsed light and a rotating magnetic field. Comprehensive laboratory analyses were conducted at specific intervals during the storage period to evaluate changes and assess the impact of storage duration on the samples. The results demonstrated that the combined use of a magnetic field and pulsed light significantly extended the shelf life of raw pork. A significant (p < 0.05) reduction in total microbial count was observed in treated samples compared to the control group throughout all storage periods. The treatment also improved all sensory attributes and reduced purge loss during refrigerated storage. Additionally, the applied treatment significantly (p < 0.05) lowered the hardness and rigidity on day 10, as well as the chewiness of the pork loin on days 1 and 10 of storage. The novelty and innovation of this study lie in the application of a rotating magnetic field combined with a pulsed light beam to enhance the properties of raw pork. This approach resulted in a synergistic effect, notably decelerating the deterioration of meat quality, extending its shelf life, and reducing energy consumption during processing. These outcomes hold significant potential for environmental, economic, and social benefits. Full article

As an important by-product of pork, pork skin can be processed into meat-based leisure food products to improve its utilization. In this study, microwave vacuum drying (MVD) technology was used to investigate the effects of microwave powers (600, 700, and 800 W) and processing duration on the drying characteristics and quality attributes of pork skin crisps (PSC). Five classical drying models were used to non-linearly fit the experimental data, and the Midilli et al. model was suitable for characterizing the MVD process of PSC. Before reaching a constant rate of drying, increasing microwave power and time can improve the brittleness and expansion ratio of PSC. In the constant rate drying stage, most of the free water in PSC was removed, showing the best brittleness and a stable expansion ratio. High power and long processing time can lead to serious lipid oxidation and change the flavor of PSC. Overall, the desired quality of PSC is recommended as 700 W for 6 min. This study can provide a reference for MVD application of meat-based by-product leisure foods. Full article

With the increasing importance of meat quality inspection, traditional manual evaluation methods face challenges in terms of efficiency and accuracy. To improve the precision and efficiency of pork quality assessment, an automated detection method based on computer vision technology is proposed for evaluating different parts and freshness of pork. First, high-resolution cameras were used to capture image data of Jinfen white pigs, covering three pork cuts—hind leg, loin, and belly—across three different collection times. These three parts were categorized into nine datasets, and the sample set was expanded through digital image processing techniques. Next, five convolutional neural network models—VGGNet, ResNet, DenseNet, MobileNet, and EfficientNet—were selected for feature recognition experiments. The experimental results showed that the MobileNetV3_Small model achieved an accuracy of 98.59%, outperforming other classical network architectures while being more lightweight. Further statistical analysis revealed that the p-values for ResNet101, EfficientNetB0, and EfficientNetB1 were all greater than 0.05, indicating that the performance differences between these models and MobileNetV3_Small were not statistically significant. In contrast, other models showed significant performance differences (p-value < 0.05). Finally, based on the PYQT5 framework, the MobileNetV3_Small model was deployed on a local client, realizing an efficient and accurate end-to-end automatic recognition system. These findings can be used to effectively enhance the efficiency and reliability of pork quality detection, providing a solid foundation for the development of pork safety monitoring systems. Full article

Post-processing contamination of Listeria monocytogenes has remained a major concern for the safety of ready-to-eat (RTE) meat products that are not reheated before consumption. Mathematical models are rapid and cost-effective tools to predict pathogen behavior, product shelf life, and safety. The objective of this study was to develop and validate a comprehensive model to predict the Listeria growth rate in RTE meat products as a function of temperature, pH, water activity, nitrite, acetic, lactic, and propionic acids. The Listeria growth data in RTE food matrices, including RTE beef, pork, and poultry products (731 data sets), were collected from the literature and databases like ComBase. The growth parameters were estimated using the logistic-with-delay primary model. The good-quality growth rate data (n = 596, R² > 0.9) were randomly divided into 80% training (n = 480) and 20% testing (n = 116) datasets. The training growth rates were used to develop a secondary gamma model, followed by validation in testing data. The growth model’s performance was evaluated by comparing the predicted and observed growth rates. The goodness-of-fit parameter of the secondary model includes R² of 0.86 and RMSE of 0.06 (μ_max) during the development stage. During validation, the gamma model with interaction included an RMSE of 0.074 (μ_max), bias, and accuracy factor of 0.95 and 1.50, respectively. Overall, about 81.03% of the relative errors (RE) of the model’s predictions were within the acceptable simulation zone (RE ± 0.5 log CFU/h). In lag time model validation, predictions were 7% fail-dangerously biased, and the accuracy factor of 2.23 indicated that the lag time prediction is challenging. The model may be used to quantify the Listeria growth in naturally contaminated RTE meats. This model may be helpful in formulations, shelf-life assessment, and decision-making for the safety of RTE meat products. Full article

Background: Pork meat is a widely consumed protein food with the potential to differentially affect health and nutritional status across social and cultural contexts. Objectives: We evaluated the association between pork meat consumption and nutrient intake, diet quality, and biomarkers of health among older adults (age ≥ 65 years) in Korea. Methods: Our analyses utilized dietary and health examination data from the 2016–2020 Korean National Health and Nutrition Examination Survey (n = 2068). Comparisons between variables derived from the nutrition survey and health examination by pork consumption (consumers vs. non-consumers) were assessed using regression analyses for survey data. Results: Pork consumption was found to be associated with younger age, greater educational attainment, and lower likelihood of living in a rural area. Consumption was also associated with a higher intake of energy and all nutrients except vitamin B6, retinol, ⍵3, and zinc in males and vitamin B6 in females. Diet quality was modestly higher among male (67.91 ± 0.93 vs. 65.74 ± 0.74; p = 0.0308) and female (70.88 ± 0.96 vs. 67.00 ± 0.73; p < 0.0001) pork consumers. Differences in biomarkers were clinically irrelevant, with inconsistencies between genders. Handgrip strength was slightly higher among male (33.84 ± 0.52 vs. 31.91 ± 0.40; p < 0.0001) and female (20.76 ± 0.34 vs. 19.99 ± 0.22; p < 0.0001) pork consumers. Conclusions: In Korean older adults, pork consumption may contribute to a higher intake of energy and most nutrients, improved diet quality scores, higher vegetable intake, and small improvements in health biomarkers. Further well-designed studies are needed to confirm these findings. Full article

The quality of pork meat directly influences the price and consumption. The genetic improvement of pigs has mainly focused on high productive efficiency, which has resulted in poor meat quality. Crossbreeds containing commercial and indigenous breeds could improve the meat quality, but identifying breed composition was difficult because of the lack of an ancestry reference panel. Therefore, we first constructed an abundant reference panel and convenient pipeline to identify ancestry/breed composition. The ancestry reference panel consisted of 517 reliable individuals, including three commercial breeds (Duroc, Landrace, and Yorkshire) and 38 indigenous Chinese breeds. The nature of the reference panel showed that the European domestic breed (EUD) and Asian domestic breed (ASD) were distinctly divided into two clusters. The evaluation of ancestry identification revealed that the reference panel performed well in identifying EUD and ASD ancestry proportions for commercial breeds, indigenous breeds, and crossbreeds. In addition, the ancestry reference panel also performed excellently in identifying breed composition for 3 commercial and 38 indigenous breeds. Specifically, the reference panel showed the outstanding identification of breed composition for crossbred individuals. These results suggested that the ancestry reference panel and convenient pipeline played a good role in identifying breed composition for pigs. Full article

In our experiment, the effect of electrical and CO₂ stunning on pork meat quality attributes was studied. A total of thirty PIC337 female pigs were allocated to two equal groups which were stunned electrically (50 Hz, 210 V, 2.6 A, 15 s) or by CO₂ (85 V% CO₂, 15 V% O₂, 90 s) and slaughtered at a commercial slaughterhouse. For 24 h post mortem, the carcass m. gluteus medius (GM) was used to evaluate meat quality attributes such as pH, color, hardness, water holding capacity (WHC), and total pigment content, and meat classification was also carried out. The CO₂ stunning resulted in muscle with significantly lower pH (p < 0.01) and poorer water holding capacity (p < 0.05) compared to electrical stunning. The GM of pigs stunned electrically showed significantly increased lightness (L*) and redness (a*) (p < 0.05) compared to CO₂-stunned. The time course of development of rigor mortis was similar for both stunning methods. Pale, soft, exudative (PSE) or dark, firm, dry (DFD) meat defects were not observed. Based on total pigment content, stunning methods did not significantly affect the amount of removable blood. Full article

Cooking with the sous vide method preserves nutritional value and maintains desirable sensory qualities of food, thanks to vacuum-sealed heat treatment at controlled low temperatures. In meat, the right combination of temperature and time is critical for texture, juiciness, and flavour, all essential features for consumer acceptance of cooked meat. This study assessed the impact of sous vide heat treatment on selected quality attributes of pork loin using various low temperature–time combinations. Pork loins were sliced, vacuum-sealed, and cooked in a water bath at temperatures of 57 °C to 63 °C for 3 to 5.5 h. The meat was evaluated for cooking loss, proximate composition, pH, water activity, oxidative changes, colour, texture, and sensory characteristics. The results showed that cooking losses increased with higher temperatures and longer times of cooking. Likewise, the oxidative changes were significantly affected by both cooking parameters. Temperature of cooking influenced only meat redness (a*), but cooking time had no significant effect on colour parameters. The lowest hardness was observed for samples cooked at 59 °C/4.5 h and at 60 °C/4–5 h. Sensory analysis indicated that cooking at 59 °C for 4.5 h provided the most acceptable sensory characteristics of pork loin. Full article

Polycyclic aromatic hydrocarbons (PAHs), carcinogenic substances primarily formed through pyrolysis and oxidation of fat at high cooking temperatures, are commonly found at high levels in grilled meats. Reducing PAHs formation by incorporating natural antioxidants, such as through marination, has been demonstrated to be effective. However, the inhibitory effect of fresh phenolic-rich fruit powders on PAHs formation in charcoal-grilled meats remains unknown. To clarify the application of the fruit powders, 15 experimental groups were conducted. All pretreatment techniques (spraying, marinating, and mixing) were applied across all four freeze-dried fruit powders (lemon, guava, papaya, and mango). Each method was systematically tested with each fruit powder to evaluate its effect on inhibiting the formation of the four PAHs (BaA, CHR, BbF, and BaP) in charcoal-grilled pork belly and loin. Firstly, guava powder exhibited the highest phenolic content and antioxidant activity compared to the lemon, papaya, and mango powders (p < 0.05), among which the main phenolic compounds were ellagic acid, quercetin, and epigallocatechin gallate (EGCG). Further, marination of pork belly with guava powder exhibited the highest inhibition rate of PAHs (94.8%), followed by lemon (91.1%), papaya (89.8%), and mango (89.0%), with a statistically significant difference at p < 0.05. The reduction in estimated daily intake (EDI) and the increase in the margin of exposure (MOE) indicate that consuming grilled meat treated with these fruit powders poses no safety concerns and may potentially reduce health risks. Finally, the sensory evaluation showed that marinating with guava powder did not perceptibly affect the sensory attributes of the meat. Overall, this study provides a potent strategy for inhibiting the formation of PAHs in meat during charcoal grilling by incorporating fruit powder while preserving sensory qualities. Full article

The fermentation process is a crucial stage in the production of salami-type sausages. However, the traditional (“warm”) method does not yield optimal results. Hence, this study evaluated the impact of fermentation technology and storage time on the quality attributes of salami-type pork sausages. “Warm”-fermented (WF) and “cold”-fermented (CF) sausages were produced using appropriate starter cultures. Following ripening, the products were stored for 63 days under cooling conditions. The evaluations encompassed microbiological and physicochemical characteristics (pH, a_w, color), basic chemical composition (NIR spectroscopy), the degree of fat hydrolysis (acid value) and oxidation (TBARS index), textural properties (TPA, shear/penetration test), and sensory (hedonic) attributes. In both sausages, mesophilic aerobes and lactic acid bacteria were the dominant microflora immediately after ripening. While the chemical composition of the salami remained constant during storage, there was a slight color change. WF sausages exhibited a significantly higher acid value and TBARS index than CF sausages. Over time, the internal structure of WF sausages became more porous, deteriorating the texture. In addition, CF sausages showed better sensory characteristics. In conclusion, using “cold” fermentation enhanced the quality and durability of salami-type sausages. This approach shows promise for industrial-scale production and aligns with consumer preferences. Full article

Research on modern agri-food chains aims to enhance flexibility by analyzing supply chain aspects to identify improvement opportunities. A SWOT analysis of 39 agri-food sector organizations using a SWOT analysis organized using the PESTEL and MEETHS categories was conducted to evaluate the stakeholders’ needs in this sector of activity in Northern Portugal. Logistic regressions were used to compute inferential statistics, which were complemented with a qualitative analysis. Cooperatives and primary sector companies often claim superior product quality without clear evidence, while corporations integrated into competitive national markets, like those with smoked products, adapt better to dietary trends. Small- and medium-sized enterprises struggle with competitive wages, leading to high turnover and difficulty retaining skilled workers. High costs hinder many organizations, particularly cooperatives, from adopting modern communication technologies affecting competitiveness. Challenges include identifying market opportunities and managing global competition for raw materials, like wild fish. Fishing and meat sectors depend heavily on modern distribution and are dominated by large retailers. Low labor costs boost competitiveness but reflect the struggle to add value. Larger organizations are more optimistic though many face challenges with the cost and volatility of key products, like pork and milk. This study offers the following key recommendations: invest in technology and innovation while balancing short-term gains with long-term sustainability; strengthen strategic planning and collaboration among corporations, cooperatives, associations, and academic institutions; and adapt to regulatory changes, invest in market and technological capabilities, and address resource limitations. Research and collaboration with policymakers and academic institutions will support tailored solutions, enabling the sector to anticipate challenges and capitalize on opportunities. Full article

The development of biopolymer-based films represents a promising direction in the packaging industry that responds to stringent needs for sustainability, reducing the ecological impact. Traditional fossil-derived polymers present major concerns because of their long decomposition time and their significant contribution to the pollution of the environment. On the contrary, biopolymers such as chitosan, PVA, and PLA offer viable alternatives. This study aimed to obtain an innovative pH indicator for smart packaging using a synthetic non-toxic anthocyanin analogue dye incorporated in bio-based films to indicate meat freshness and quality. The pH-responsive color-changing properties of the dye make it suitable for developing intelligent films to monitor food freshness. The obtained polymeric films were characterized by FT-IR and UV–VIS spectroscopy, and their thermal properties were assessed using thermogravimetric methods. Moisture content, swelling capacity, and water solubility of the polymeric films were also evaluated. The sensitivity of the biopolymer–flavylium composite films to pH variations was studied in the pH range of 2 to 12 and noticeable color variations were observed, allowing the monitoring of the meat’s quality damage through pH changes. The pH-responsive films were applied directly on the surface or in the proximity of pork and chicken meat samples, to evaluate their colorimetric response to fresh and spoilt meat. This study can be the starting point for creating more durable packaging solutions leading to a circular economy. Full article

Pork belly, prized for its unique flavor and texture, is often overlooked in breeding programs that prioritize lean meat production. The quality of pork belly is determined by the number and distribution of muscle and fat layers. This study aimed to assess the number of pork belly layers using deep learning techniques. Initially, semantic segmentation was considered, but the intersection over union (IoU) scores for the segmented parts were below 70%, which is insufficient for practical application. Consequently, the focus shifted to image classification methods. Based on the number of fat and muscle layers, a dataset was categorized into three groups: three layers (n = 1811), five layers (n = 1294), and seven layers (n = 879). Drawing upon established model architectures, the initial model was refined for the task of learning and predicting layer traits from B-ultrasound images of pork belly. After a thorough evaluation of various performance metrics, the ResNet18 model emerged as the most effective, achieving a remarkable training set accuracy of 99.99% and a validation set accuracy of 96.22%, with corresponding loss values of 0.1478 and 0.1976. The robustness of the model was confirmed through three interpretable analysis methods, including grad-CAM, ensuring its reliability. Furthermore, the model was successfully deployed in a local setting to process B-ultrasound video frames in real time, consistently identifying the pork belly layer count with a confidence level exceeding 70%. By employing a scoring system with 100 points as the threshold, the number of pork belly layers in vivo was categorized into superior and inferior grades. This innovative system offers immediate decision-making support for breeding determinations and presents a highly efficient and precise method for assessment of pork belly layers. Full article