Search Results (1,598)

Background: The current H3N2 influenza subunit vaccine exhibits weak immunogenicity, which limits its effectiveness in preventing and controlling influenza virus infections. Methods: In this study, we aimed to develop a T4 phage-based nanovaccine designed to enhance the immunogenicity of two antigens by displaying the HA1 and M2e antigens of the H3N2 influenza virus on each phage nanoparticle. Specifically, we fused the Soc protein with the HA1 antigen and the Hoc protein with the M2e antigen, assembling them onto a T4 phage that lacks Soc and Hoc proteins (Soc⁻Hoc⁻T4), thereby constructing a nanovaccine that concurrently presents both HA1 and M2e antigens. Results: The analysis of the optical density of the target protein bands indicated that each particle could display approximately 179 HA1 and 68 M2e antigen molecules. Additionally, animal experiments demonstrated that this nanoparticle vaccine displaying dual antigen clusters induced a stronger specific immune response, higher antibody titers, a more balanced Th1/Th2 immune response, and enhanced CD4⁺ and CD8⁺ T cell effects compared to immunization with HA1 and M2e antigen molecules alone. Importantly, mice immunized with the T4 phage displaying dual antigen clusters achieved full protection (100% protection) against the H3N2 influenza virus, highlighting its robust protective efficacy. Conclusions: In summary, our findings indicate that particles based on a T4 phage displaying antigen clusters exhibit ideal immunogenicity and protective effects, providing a promising strategy for the development of subunit vaccines against various viruses beyond influenza. Full article

Low-carbon pilot policies are essential for the green transformation of resource-based cities, helping them mitigate the “carbon curse” and the “resource curse” while promoting sustainable socio-ecological development. Focusing on a panel of 114 resource-based cities in China, spanning from 2003 to 2022, this study employs a range of methodologies, including kernel density estimation, the Difference-in-Differences Model, Spatial Difference-in-Differences, Mediation Analysis, K-means Clustering, and Dual Machine Learning to assess the consequences of low-carbon pilot policies on socio-ecological resilience. The findings indicate that the socio-ecological resilience of the study area has generally improved, though there is noticeable polarization. Low-carbon pilot policies significantly enhance the resilience of resource-based cities by 0.4%, and they exhibit a positive spatial spillover effect of 1.1%. However, the long-term effects of the policies on economic resilience were not significant, and the policies did not have a direct impact on the social resilience of the pilot cities; however, they did promote social resilience in neighboring regions. Finally, the effectiveness of low-carbon pilots varies, with more pronounced benefits in declining and mature resource cities, particularly in those with medium ecological and economic resilience, and low social resilience. Green finance, industrial transformation, and carbon emission efficiency are identified as key strategies for improving socio-ecological resilience. The above findings provide insights for policymakers seeking to foster inclusive, resilient, and sustainable urban development in China. Full article

Pharmacogenetics is a branch of genomic medicine aiming to personalize drug prescription guidelines based on individual genetic information. This concept might lead to a reduction in adverse drug reactions, which place a heavy burden on individual patients’ health and the economy of the healthcare system. The aim of this study was to present insights gained from the pharmacogenetics-based clustering of over 500 patients from the Croatian population. The data used in this article were obtained by the pharmacogenetic testing of 522 patients from the Croatian population. The patients were clustered based on the genotypes of 28 pharmacologically relevant genes. Dimensionality reduction was employed using the UMAP algorithm, after which clusters were defined using HDBSCAN. Validation of clustering was performed by decision tree analysis and predictive modeling using the RandomForest, XGBoost, and ExtraTrees classification algorithms. The clustering algorithm defined six clusters of patients based on two UMAP components (silhouette score = 0.782). Decision tree analysis demonstrated CYP2D6 and SLCO1B1 genotypes as the main points of cluster determination. Predictive modeling demonstrated an excellent ability to discern the cluster of each patient based on all genes (avg. ROC-AUC = 0.998), CYP2D6 and SLCO1B1 (avg. ROC-AUC = 1.000), and CYP2D6 alone (avg. ROC-AUC = 0.910). Membership in each cluster provided clinically relevant information, in the context of ruling out certain favorable or unfavorable phenotypes. However, this study’s main limitation is its cohort size. Through further research and investigation of a larger number of patients, more accurate and clinically applicable associations between pharmacogenetic genotypes and phenotypes might be discovered. Full article

Technological developments have allowed helicopter airborne laser scanning (HALS) to produce high-density point clouds below the forest canopy. We present a tree stem classification method that combines linear shape detection and model-based clustering, using four discrete methods to estimate stem diameter. Stem horizontal size was estimated every 25 cm below the living crown, and a cubic spline was used to estimate where there were gaps. Individual stem diameter at breast height (DBH) was estimated for 77% of field-measured trees. The root mean square error (RMSE) of DBH estimates was 7–12 cm using stem circle fitting. Adapting the approach to use an existing stem taper model reduced the RMSE of estimates (<1 cm). In contrast, estimates that were produced from a previously existing DBH estimation method (PREV) could be achieved for 100% of stems (DBH RMSE 6 cm), but only after location-specific error was corrected. The stem classification method required comparatively little development of statistical models to provide estimates, which ultimately had a similar level of accuracy (RMSE < 1 cm) to PREV. HALS datasets can measure broad-scale forest plantations and reduce field efforts and should be considered an important tool for aiding in inventory creation and decision-making within forest management. Full article

A protein complex can be regarded as a functional module developed by interacting proteins. The protein complex has attracted significant attention in bioinformatics as a critical substance in life activities. Identifying protein complexes in protein–protein interaction (PPI) networks is vital in life sciences and biological activities. Therefore, significant efforts have been made recently in biological experimental methods and computing methods to detect protein complexes accurately. This study proposed a new method for PPI networks to facilitate the processing and development of the following algorithms. Then, a combination of the improved density peaks clustering algorithm (DPC) and the fuzzy C-means clustering algorithm (FCM) was proposed to overcome the shortcomings of the traditional FCM algorithm. In other words, the rationality of results obtained using the FCM algorithm is closely related to the selection of cluster centers. The objective function of the FCM algorithm was redesigned based on ‘high cohesion’ and ‘low coupling’. An adaptive parameter-adjusting algorithm was designed to optimize the parameters of the proposed detection algorithm. This algorithm is denoted as the DFPO algorithm (DPC-FCM Parameter Optimization). Finally, the performance of the DFPO algorithm was evaluated using multiple metrics and compared with over ten state-of-the-art protein complex detection algorithms. Experimental results indicate that the proposed DFPO algorithm exhibits improved detection accuracy compared with other algorithms. Full article

Automatic Identification System (AIS) data offer essential insights into maritime traffic patterns; however, effective visualization tools for decision-making remain limited. This study presents an integrated visualization processing method to support ship operators by identifying maritime traffic behavior information, such as traffic density, direction, and flow in specific sea navigational areas. We analyzed AIS dynamic data from a specific sea area, calculated ship density distributions across a grid lattice, and obtained visualizations of traffic-dense areas as heat maps. Using the density-based spatial clustering of applications with a noise algorithm, we detected traffic direction at each grid point, which was visualized in the form of directional arrows, and clustered ship trajectories to identify representative traffic flows. The visualizations were integrated and overlaid onto an S-57-based electronic nautical map for Mokpo’s entry and exit routes, revealing primary shipping lanes and critical inflection points within the target area. This integrated visualization method simultaneously displays traffic density, flow, and customary routes. It is adapted for the electronic nautical chart (S-101) under the next-generation hydrographic information standard (S-100), which can be used as a tool to support decision-making for ship operators. Full article

Al-Li alloys are widely used in aerospace applications due to their high strength, high fracture toughness, and strong resistance to stress corrosion. However, the lack of interatomic potentials has hindered systematic investigations of the relationship between structures and properties. To address this issue, we apply a neural network-based neuroevolutionary machine learning potential (NEP) and use evolutionary strategies to train it for large-scale molecular dynamics (MD) simulations. The results obtained from this potential function are compared with those from Density Functional Theory (DFT) calculations, with training errors of 2.1 meV/atom for energy, 47.4 meV/Å for force, and 14.8 meV/atom for virial, demonstrating high training accuracy. Using this potential, we simulate cluster formation and the high-temperature stability of the T1 phase, with results consistent with previous experimental findings, confirming the accurate predictive capability of this potential. This approach provides a simple and efficient method for predicting atomic motion, offering a promising tool for the thermal treatment of Al-Li alloys. Full article

The localization of gambling establishments is a relevant topic in gambling research. In this paper, we analyze the spatial distribution of two types of gambling establishments—private and public—over the last 10 years in the municipality of Madrid (Spain). Using a spatial scan statistic, we identify the temporal dynamics of spatial clusters with high densities. The results reveal different spatial patterns regarding the locations of these two types of gambling establishments. While public gambling establishments do not exhibit spatial clustering, private gambling establishments show a growth in spatial clustering with dynamic behavior, seeking locations with specific sociodemographic characteristics. A machine learning tree-based algorithm is used to confirm that decisions on where to put new gambling establishments are based on targeting customers with a gambling profile. Full article

This study investigates the electrochemical conversion of methane to methanol using fuel-cell-type reactors with palladium- and lanthanum-based catalysts supported on antimony-doped tin oxide (ATO). The combination of these elements demonstrated promising characteristics for selective methanol production. Transmission electron microscopy (TEM) analysis revealed the impact of lanthanum addition on palladium nanoparticles, influencing size distribution and clusters. Polarization curves and power density plots highlighted the Pd₅₀La₅₀/ATO catalyst, indicating an optimal palladium/lanthanum ratio for methanol optimization. FTIR analysis confirmed the presence of methanol in the reaction products, while the methanol production rate showcased the superior performance of the Pd₅₀La₅₀/ATO catalyst compared to other compositions. The synergistic effects between lanthanum’s water activation capability and the carbophilic nature of PdO emerged as crucial factors for the catalyst’s success. Full article

The main categories of transition metal–mercury heterometallic compounds are briefly summarized. The attention is focused on complexes and clusters where the {Hg-Y} fragment, where Y represents a halide atom, interacts with transition metals. Most of the structurally characterized derivatives are organometallic compounds where the transition metals belong to the Groups 6, 8, 9 and 10. More than one {Hg-Y} group can be present in the same compound, interacting with the same or with different transition metals. The main synthetic strategies are discussed, and structural data of representative compounds are reported. According to the isolobality with hydrogen, {Hg-Y} can form from one to three M-{Hg-Y} bonds, but further interactions can be present, such as mercurophilic and Hg···halide contacts. The formal oxidation state of mercury is sometimes ambiguous and thus {Hg-Y} can be considered as a Lewis acid or base on varying the transition metal fragment. Density functional theory calculations on selected Group 6 and Group 9 model compounds are provided in order to shed light on this aspect. Full article

The geometric feature characterization of fruit trees plays a role in effective management in orchards. LiDAR (light detection and ranging) technology for object detection enables the rapid and precise evaluation of geometric features. This study aimed to quantify the height, canopy volume, tree spacing, and row spacing in an apple orchard using a three-dimensional (3D) LiDAR sensor. A LiDAR sensor was used to collect 3D point cloud data from the apple orchard. Six samples of apple trees, representing a variety of shapes and sizes, were selected for data collection and validation. Commercial software and the python programming language were utilized to process the collected data. The data processing steps involved data conversion, radius outlier removal, voxel grid downsampling, denoising through filtering and erroneous points, segmentation of the region of interest (ROI), clustering using the density-based spatial clustering (DBSCAN) algorithm, data transformation, and the removal of ground points. Accuracy was assessed by comparing the estimated outputs from the point cloud with the corresponding measured values. The sensor-estimated and measured tree heights were 3.05 ± 0.34 m and 3.13 ± 0.33 m, respectively, with a mean absolute error (MAE) of 0.08 m, a root mean squared error (RMSE) of 0.09 m, a linear coefficient of determination (r²) of 0.98, a confidence interval (CI) of −0.14 to −0.02 m, and a high concordance correlation coefficient (CCC) of 0.96, indicating strong agreement and high accuracy. The sensor-estimated and measured canopy volumes were 13.76 ± 2.46 m³ and 14.09 ± 2.10 m³, respectively, with an MAE of 0.57 m³, an RMSE of 0.61 m³, an r² value of 0.97, and a CI of −0.92 to 0.26, demonstrating high precision. For tree and row spacing, the sensor-estimated distances and measured distances were 3.04 ± 0.17 and 3.18 ± 0.24 m, and 3.35 ± 0.08 and 3.40 ± 0.05 m, respectively, with RMSE and r² values of 0.12 m and 0.92 for tree spacing, and 0.07 m and 0.94 for row spacing, respectively. The MAE and CI values were 0.09 m, 0.05 m, and −0.18 for tree spacing and 0.01, −0.1, and 0.002 for row spacing, respectively. Although minor differences were observed, the sensor estimates were efficient, though specific measurements require further refinement. The results are based on a limited dataset of six measured values, providing initial insights into geometric feature characterization performance. However, a larger dataset would offer a more reliable accuracy assessment. The small sample size (six apple trees) limits the generalizability of the findings and necessitates caution in interpreting the results. Future studies should incorporate a broader and more diverse dataset to validate and refine the characterization, enhancing management practices in apple orchards. Full article

In recent years, China’s population growth rate is slowing, and birth rates are declining. The impact of changes in population size and structure on logistics development has raised important concerns. The coupling coordination between population and logistics has significant implications for the sustainable development of regional economies. This paper analyzes the relationship between population changes and logistics development from the perspective of the spatiotemporal evolution of their coupling coordination. First, based on population and logistics data from 31 provincial-level administrative regions in China from 2003 to 2022, a comprehensive evaluation indicator system is established to assess the population and logistics development levels across these provinces. Second, a coupling coordination degree model is constructed to explore the coupling coordination relationship between population changes and logistics development. Methods such as kernel density estimation and spatial autocorrelation analysis are employed to analyze the temporal evolution characteristics of the coupling coordination degree of the two subsystems. The results indicate that: (1) From 2003 to 2022, the population and logistics development levels in 31 provinces exhibited an upward trend. The growth rate of logistics development level scores exceeds that of population scores; (2) During the study period, the coupling coordination degree between population and logistics in most provinces shows a year-on-year increase, and notable spatial differences exhibit a decreasing trend; (3) There exists a significant positive spatial correlation in the coupling coordination degree between population and logistics across the 31 provinces, and local spatial autocorrelation is primarily characterized by ‘high–high’ and ‘low–low’ clustering distributions. Based on the above results, we propose policy recommendations to promote the coordinated development of population and logistics. Full article

The high mobility and dynamic nature of unmanned aerial vehicles (UAVs) pose significant challenges to clustering and routing in flying ad hoc networks (FANETs). Traditional methods often fail to achieve stable networks with efficient resource utilization and low latency. To address these issues, we propose a hybrid bio-inspired algorithm, HMAO, combining the mountain gazelle optimizer (MGO) and the aquila optimizer (AO). HMAO improves cluster stability and enhances data delivery reliability in FANETs. The algorithm uses MGO for efficient cluster head (CH) selection, considering UAV energy levels, mobility patterns, intra-cluster distance, and one-hop neighbor density, thereby reducing re-clustering frequency and ensuring coordinated operations. For cluster maintenance, a congestion-based approach redistributes UAVs in overloaded or imbalanced clusters. The AO-based routing algorithm ensures reliable data transmission from CHs to the base station by leveraging predictive mobility data, load balancing, fault tolerance, and global insights from ferry nodes. According to the simulations conducted on the network simulator (NS-3.35), the HMAO technique exhibits improved cluster stability, packet delivery ratio, low delay, overhead, and reduced energy consumption compared to the existing methods. Full article

Background: The diagnostic challenges presented by hyperchromatic crowded cell groups (HCGs) in cervical cytology often result in either overdiagnosis or underdiagnosis due to their densely packed, three-dimensional structures. The objective of this study is to characterize the structural differences among HSIL-HCGs, AGC-HCGs, and NILM-HCGs using quantitative texture analysis metrics, with the aim of facilitating the differentiation of benign from malignant cases. Methods: A total of 585 HCGs images were analyzed, with assessments conducted on 8-bit gray-scale value, thickness, skewness, and kurtosis across various groups. Results: HSIL-HCGs are distinctly classified based on 8-bit gray-scale value. Significant statistical differences were observed in all groups, with HSIL-HCGs exhibiting higher cellular density and cluster thickness compared to NILM and AGC groups. In the AGC group, HCGs shows statistically significant differences in 8-bit gray-scale value compared to NILM-HCGs, but the classification performance by 8-bit gray-scale value is not high because the cell density and thickness are almost similar. These variations reflect the characteristic cellular structures unique to each group and substantiate the potential of 8-bit gray-scale value as an objective diagnostic indicator, especially for HSIL-HCGs. Conclusion: Our findings indicate that the integration of gray-scale-based texture analysis has the potential to improve diagnostic accuracy in cervical cytology and break through current diagnostic limitations in the identification of high-risk lesions. Full article

Intelligent ships are a key focus for the future development of maritime transportation, relying on efficient decision-making and autonomous control within complex environments. To enhance the perception, prediction, and decision-making capabilities of these ships, the present study proposes a novel approach for constructing a time-series knowledge graph, utilizing real-time Automatic Identification System (AIS) data analyzed via a sliding window technique. By integrating advanced technologies such as knowledge extraction, representation learning, and semantic fusion, both static and dynamic navigational data are systematically unified within the knowledge graph. The study specifically targets the extraction and modeling of critical events, including variations in ship speed, course changes, vessel encounters, and port entries and exits. To evaluate the urgency of encounters, mathematical algorithms are applied to the Distance to Closest Point of Approach (DCPA) and Time to Closest Point of Approach (TCPA) metrics. Furthermore, the DBSCAN (Density-Based Spatial Clustering of Applications with Noise) clustering algorithm is employed to identify suitable docking berths. Additionally, multi-source meteorological data are integrated with ship dynamic data, providing a more comprehensive representation of the maritime environment. The resulting knowledge system effectively combines ship attributes, navigational status, event relationships, and environmental factors, thereby offering a robust framework for supporting intelligent ship operations. Full article