Search Results (19)

This study delves into the analysis of a vineyard in Carinthia, Austria, focusing on the automated derivation of ecosystem structures of individual vine parameters, including vine heights, leaf area index (LAI), leaf surface area (LSA), and the geographic positioning of single plants. For the derivation of these parameters, intricate segmentation processes and nuanced UAS-based data acquisition techniques are necessary. The detection of single vines was based on 3D point cloud data, generated at a phenological stage in which the plants were in the absence of foliage. The mean distance from derived vine locations to reference measurements taken with a GNSS device was 10.7 cm, with a root mean square error (RMSE) of 1.07. Vine height derivation from a normalized digital surface model (nDSM) using photogrammetric data showcased a strong correlation (R² = 0.83) with real-world measurements. Vines underwent automated classification through an object-based image analysis (OBIA) framework. This process enabled the computation of ecosystem structures at the individual plant level post-segmentation. Consequently, it delivered comprehensive canopy characteristics rapidly, surpassing the speed of manual measurements. With the use of uncrewed aerial systems (UAS) equipped with optical sensors, dense 3D point clouds were computed for the derivation of canopy-related ecosystem structures of vines. While LAI and LSA computations await validation, they underscore the technical feasibility of obtaining precise geometric and morphological datasets from UAS-collected data paired with 3D point cloud analysis and object-based image analysis. Full article

The microbial communities of the rhizospheres of vineyards have been subject to a considerable body of research, but it is still unclear how the applied soil cultivation methods are able to change the structure, composition, and level of diversity of their communities. Rhizosphere samples were collected from three neighbouring vineyards with the same time of planting and planting material (rootstock: Teleki 5C; Vitis vinifera: Müller Thurgau). Our objective was to examine the diversity occurring in bacterial community structures in vineyards that differ only in the methods of tillage procedure applied, namely intensive (INT), extensive (EXT), and abandoned (AB). For that we took samples from two depths (10–30 cm (shallow = S) and 30–50 cm (deep = D) of the grape rhizosphere in each vineyard and the laboratory and immediately prepared the slices of the roots for DNA-based analysis of the bacterial communities. Bacterial community structure was assessed by means of PCR-DGGE analysis carried out on the v3 region of 16S rRNA gene. Based on the band composition of the DGGE profiles thus obtained, the diversity of the microbial communities was evaluated and determined by the Shannon–Weaver index (H′). Between the AB and EXT vineyards at the S depth, the similarity of the community structure was 55%; however, the similarity of the D samples was more than 80%, while the difference between the INT samples and the other two was also higher than 80%. Based on our results, we can conclude that intensive cultivation strongly affects the structure and diversity of the bacterial community. Full article

A three-year survey was conducted to estimate the incidence of grapevine trunk diseases (GTDs) in Greece and identify fungi associated with the disease complex. In total, 310 vineyards in different geographical regions in northern, central, and southern Greece were surveyed, and 533 fungal strains were isolated from diseased vines. Morphological, physiological and molecular (5.8S rRNA gene-ITS sequencing) analyses revealed that isolates belonged to 35 distinct fungal genera, including well-known (e.g., Botryosphaeria sp., Diaporthe spp., Eutypa sp., Diplodia sp., Fomitiporia sp., Phaeoacremonium spp., Phaeomoniella sp.) and lesser-known (e.g., Neosetophoma sp., Seimatosporium sp., Didymosphaeria sp., Kalmusia sp.) grapevine wood inhabitants. The GTDs-inducing population structure differed significantly among the discrete geographical zones. Phaeomoniella chlamydospora (26.62%, n = 70), Diaporthe spp. (18.25%, n = 48) and F. mediterranea (10.27%, n = 27) were the most prevalent in Heraklion, whereas D. seriata, Alternaria spp., P. chlamydospora and Fusarium spp. were predominant in Nemea (central Greece). In Amyntaio and Kavala (northern Greece), D. seriata was the most frequently isolated species (>50% frequency). Multi-genes (rDNA-ITS, LSU, tef1-α, tub2, act) sequencing of selected isolates, followed by pathogenicity tests, revealed that Neosetophoma italica, Seimatosporium vitis, Didymosphaeria variabile and Kalmusia variispora caused wood infection, with the former being the most virulent. To the best of our knowledge, this is the first report of N. italica associated with GTDs worldwide. This is also the first record of K. variispora, S. vitis and D. variabile associated with wood infection of grapevine in Greece. The potential associations of disease indices with vine age, cultivar, GTD-associated population structure and the prevailing meteorological conditions in different viticultural zones in Greece are presented and discussed. Full article

Fungicide applications constitute a management practice that reduces the size of fungal populations and by acting as a genetic drift factor, may affect pathogen evolution. In a previous study, we showed that the farming system influenced the population structure of the Aspergillus section Nigri species in Greek vineyards. The current study aimed to test the hypothesis that the differences in the population structure may be associated with the selection of fungicide-resistant strains within the black aspergilli populations. To achieve this, we determined the sensitivity of 102, 151, 19, and 22 for the A. uvarum, A. tubingensis, A. niger, and A. carbonarious isolates, respectively, originating either from conventionally-treated or organic vineyards to the fungicides fluxapyroxad-SDHIs, pyraclostrobin-QoIs, tebuconazole-DMIs, and fludioxonil-phenylpyrroles. The results showed widespread resistance to all four fungicides tested in the A. uvarum isolates originating mostly from conventional vineyards. In contrast, all the A. tubingensis isolates tested were sensitive to pyraclostrobin, while moderate frequencies of only lowly resistant isolates were identified for tebuconazole, fludioxonil, and fluxapyroxad. Sequencing analysis of the corresponding fungicide target encoding genes revealed the presence of H270Y, H65Q/S66P, and G143A mutations in the sdhB, sdhD, and cytb genes of A. uvarum resistant isolates, respectively. No mutations in the Cyp51A and Cyp51B genes were detected in either the A. uvarum or A. tubingensis isolates exhibiting high or low resistance levels to DMIs, suggesting that other resistance mechanisms are responsible for the observed phenotype. Our results support the initial hypothesis for the contribution of fungicide resistance in the black aspergilli population structure in conventional and organic vineyards, while this is the first report of A. uvarum resistance to SDHIs and the first documentation of H270Y or H65Q/S66P mutations in sdhB, sdhD, and of the G143A mutation in the cytb gene of this fungal species. Full article

In this paper, we present a complete and efficient solution of guidance, navigation and control for a quadrotor platform to accomplish 3D coverage flight missions in mapped vineyard terrains. Firstly, an occupancy grid map of the terrain is used to generate a safe guiding coverage path using an Iterative Structured Orientation planning algorithm. Secondly, way-points are extracted from the generated path and added to them trajectory’s velocities and accelerations constraints. The constrained way-points are fed into a Linear Quadratic Regulator algorithm so as to generate global minimum snap optimal trajectory while satisfying both the pointing and the corridor constraints. Then, when facing unexpected obstacles, the quadrotor tends to re-plan its path in real-time locally using an Improved Artificial Potential Field algorithm. Finally, a geometric trajectory tracking controller is developed on the Special Euclidean group SE(3). The aim of this controller is to track the generated trajectory while pointing towards predetermined direction using the vector measurements provided by the inertial unit. The performance of the proposed method is demonstrated through several simulation results. In particular, safe guiding paths are achieved. Obstacle-free optimal trajectories that satisfy the way-point position, the pointing direction, and the corridor constraints, are successfully generated with optimized platform snap. Besides, the implemented geometric controller can achieve higher trajectory tracking accuracy with an absolute value of the maximum error in the order of 10−3 m. Full article

Grapevine virus A (GVA) is a phloem-restricted virus (genus Vitivirus, family Betaflexiviridae) that cause crop losses of 5–22% in grapevine cultivars, transmitted by different species of pseudococcid mealybugs, the mealybug Heliococcus bohemicus, and by the scale insect Neopulvinaria innumerabilis. In this work, we studied the genetic structure and molecular variability of GVA, ascertaining its presence and spread in different commercial vineyards of four Sicilian provinces (Italy). In total, 11 autochthonous grapevine cultivars in 20 commercial Sicilian vineyards were investigated, for a total of 617 grapevine samples. Preliminary screening by serological (DAS-ELISA) analysis for GVA detection were conducted and subsequently confirmed by molecular (RT-PCR) analysis. Results showed that 10 out of the 11 cultivars analyzed were positive to GVA, for a total of 49 out of 617 samples (8%). A higher incidence of infection was detected on ‘Nerello Mascalese’, ‘Carricante’, ‘Perricone’ and ‘Nero d’Avola’ cultivars, followed by ‘Alicante’, ‘Grecanico’, ‘Catarratto’, ‘Grillo’, ‘Nerello Cappuccio’ and ‘Zibibbo’, while in the ‘Moscato’ cultivar no infection was found. Phylogenetic analyses carried out on the coat protein (CP) gene of 16 GVA sequences selected in this study showed a low variability degree among the Sicilian isolates, closely related with other Italian isolates retrieved in GenBank, suggesting a common origin, probably due to the exchange of infected propagation material within the Italian territory. Full article

In the field of computer vision, 3D reconstruction of crops plays a crucially important role in agriculture. On-ground assessment of geometrical features of vineyards is of vital importance to generate valuable information that enables producers to take the optimum actions in terms of agricultural management. A training system of vines (Vitis vinifera L.), which involves pruning and a trellis system, results in a particular vine architecture, which is vital throughout the phenological stages. Pruning is required to maintain the vine’s health and to keep its productivity under control. The creation of 3D models of vineshoots is of crucial importance for management planning. Volume and structural information can improve pruning systems, which can increase crop yield and improve crop management. In this experiment, an RGB-D camera system, namely Kinect v2, was used to reconstruct 3D vine models, which were used to determine shoot volume on eight differentiated vineyard training systems: Lyre, GDC (Geneva Double Curtain), Y-Trellis, Pergola, Single Curtain, Smart Dyson, VSP (Vertical Shoot Positioned), and the head-trained Gobelet. The results were compared with dry biomass ground truth-values. Dense point clouds had a substantial impact on the connection between the actual biomass measurements in four of the training systems (Pergola, Curtain, Smart Dyson and VSP). For the comparison of actual dry biomass and RGB-D volume and its associated 3D points, strong linear fits were obtained. Significant coefficients of determination (R² = 0.72 to R² = 0.88) were observed according to the number of points connected to each training system separately, and the results revealed good correlations with actual biomass and volume values. When comparing RGB-D volume to weight, Pearson’s correlation coefficient increased to 0.92. The results reveal that the RGB-D approach is also suitable for shoot reconstruction. The research proved how an inexpensive optical sensor can be employed for rapid and reproducible 3D reconstruction of vine vegetation that can improve cultural practices such as pruning, canopy management and harvest. Full article

In precision viticulture, the intra-field spatial variability characterization is a crucial step to efficiently use natural resources by lowering the environmental impact. In recent years, technologies such as Unmanned Aerial Vehicles (UAVs), Mobile Laser Scanners (MLS), multispectral sensors, Mobile Apps (MA) and Structure from Motion (SfM) techniques enabled the possibility to characterize this variability with low efforts. The study aims to evaluate, compare and cross-validate the potentiality and the limits of several tools (UAV, MA, MLS) to assess the vine canopy size parameters (thickness, height, volume) by processing 3D point clouds. Three trials were carried out to test the different tools in a vineyard located in the Chianti Classico area (Tuscany, Italy). Each test was made of a UAV flight, an MLS scanning over the vineyard and a MA acquisition over 48 geo-referenced vines. The Leaf Area Index (LAI) were also assessed and taken as reference value. The results showed that the analyzed tools were able to correctly discriminate between zones with different canopy size characteristics. In particular, the R² between the canopy volumes acquired with the different tools was higher than 0.7, being the highest value of R² = 0.78 with a RMSE = 0.057 m³ for the UAV vs. MLS comparison. The highest correlations were found between the height data, being the highest value of R² = 0.86 with a RMSE = 0.105 m for the MA vs. MLS comparison. For the thickness data, the correlations were weaker, being the lowest value of R² = 0.48 with a RMSE = 0.052 m for the UAV vs. MLS comparison. The correlation between the LAI and the canopy volumes was moderately strong for all the tools with the highest value of R² = 0.74 for the LAI vs. V_MLS data and the lowest value of R² = 0.69 for the LAI vs. V_UAV data. Full article

More than 100 grapevine varieties are registered as suitable for wine production in “Douro” and “Trás-os-Montes” Protected Designations of Origin regions; however, only a few are actually used for winemaking. The identification of varieties cultivated in past times can be an important step to take advantage of all the potential of these regions grape biodiversity. The conservation of the vanishing genetic resources boosts greater product diversification, and it can be considered strategic in the valorisation of these wine regions. Hence, one goal of the present study was to prospect and characterise, through molecular markers, 310 plants of 11 old vineyards that constitute a broad representation of the grape genetic patrimony of “Douro” and “Trás-os-Montes” wine regions; 280 samples, grouped into 52 distinct known varieties, were identified through comparison of their genetic profiles generated via 6 nuclear SSR and 43 informative SNP loci amplification; the remaining 30 samples, accounting for 13 different genotypes, did not match with any profile in the consulted databases and were considered as new genotypes. This study also aimed at evaluating the population structure among the 65 non-redundant genotypes identified, which were grouped into two ancestral genetic groups. The mean probability of identity values of 0.072 and 0.510 (for the 6 SSR and 226 SNP sets, respectively) were determined. Minor differences were observed between frequencies of chlorotypes A and D within the non-redundant genotypes studied. Twenty-seven pedigrees were confirmed and nine new trios were established. Ancestors of eight genotypes remain unknown. Full article

In viticulture, information about vine vigour is a key input for decision-making in connection with production targets. Pruning weight (PW), a quantitative variable used as indicator of vegetative vigour, is associated with the quantity and quality of the grapes. Interest has been growing in recent years around the use of unmanned aerial vehicles (UAVs) or drones fitted with remote sensing facilities for more efficient crop management and the production of higher quality wine. Current research has shown that grape production, leaf area index, biomass, and other viticulture variables can be estimated by UAV imagery analysis. Although SfM lowers costs, saves time, and reduces the amount and type of resources needed, a review of the literature revealed no studies on its use to determine vineyard pruning weight. The main objective of this study was to predict PW in vineyards from a 3D point cloud generated with RGB images captured by a standard drone and processed by SfM. In this work, vertical and oblique aerial images were taken in two vineyards of Godello and Mencía varieties during the 2019 and 2020 seasons using a conventional Phantom 4 Pro drone. Pruning weight was measured on sampling grids comprising 28 calibration cells for Godello and 59 total cells for Mencía (39 calibration cells and 20 independent validation). The volume of vegetation (V) was estimated from the generated 3D point cloud and PW was estimated by linear regression analysis taking V as predictor variable. When the results were leave-one-out cross-validated (LOOCV), the R² was found to be 0.71 and the RMSE 224.5 (g) for the PW estimate in Mencía 2020, calculated for the 39 calibration cells on the grounds of oblique images. The regression analysis results for the 20 validation samples taken independently of the rest (R² = 0.62; RMSE = 249.3 g) confirmed the viability of using the SfM as a fast, non-destructive, low-cost procedure for estimating pruning weight. Full article

In the past decades, technology-based agriculture, also known as Precision Agriculture (PA) or smart farming, has grown, developing new technologies and innovative tools to manage data for the whole agricultural processes. In this framework, geographic information, and spatial data and tools such as UAVs (Unmanned Aerial Vehicles) and multispectral optical sensors play a crucial role in the geomatics as support techniques. PA needs software to store and process spatial data and the Free and Open Software System (FOSS) community kept pace with PA’s needs: several FOSS software tools have been developed for data gathering, analysis, and restitution. The adoption of FOSS solutions, WebGIS platforms, open databases, and spatial data infrastructure to process and store spatial and nonspatial acquired data helps to share information among different actors with user-friendly solutions. Nevertheless, a comprehensive open-source platform that, besides processing UAV data, allows directly storing, visualising, sharing, and querying the final results and the related information does not exist. Indeed, today, the PA’s data elaboration and management with a FOSS approach still require several different software tools. Moreover, although some commercial solutions presented platforms to support management in PA activities, none of these present a complete workflow including data from acquisition phase to processed and stored information. In this scenario, the paper aims to provide UAV and PA users with a FOSS-replicable methodology that can fit farming activities’ operational and management needs. Therefore, this work focuses on developing a totally FOSS workflow to visualise, process, analyse, and manage PA data. In detail, a multidisciplinary approach is adopted for creating an operative web-sharing tool able to manage Very High Resolution (VHR) agricultural multispectral-derived information gathered by UAV systems. A vineyard in Northern Italy is used as an example to show the workflow of data generation and the data structure of the web tool. A UAV survey was carried out using a six-band multispectral camera and the data were elaborated through the Structure from Motion (SfM) technique, resulting in 3 cm resolution orthophoto. A supervised classifier identified the phenological stage of under-row weeds and the rows with a 95% overall accuracy. Then, a set of GIS-developed algorithms allowed Individual Tree Detection (ITD) and spectral indices for monitoring the plant-based phytosanitary conditions. A spatial data structure was implemented to gather the data at canopy scale. The last step of the workflow concerned publishing data in an interactive 3D webGIS, allowing users to update the spatial database. The webGIS can be operated from web browsers and desktop GIS. The final result is a shared open platform obtained with nonproprietary software that can store data of different sources and scales. Full article

A non-destructive measuring technique was applied to test major vine geometric traits on measurements collected by a contactless sensor. Three-dimensional optical sensors have evolved over the past decade, and these advancements may be useful in improving phenomics technologies for other crops, such as woody perennials. Red, green and blue-depth (RGB-D) cameras, namely Microsoft Kinect, have a significant influence on recent computer vision and robotics research. In this experiment an adaptable mobile platform was used for the acquisition of depth images for the non-destructive assessment of branch volume (pruning weight) and related to grape yield in vineyard crops. Vineyard yield prediction provides useful insights about the anticipated yield to the winegrower, guiding strategic decisions to accomplish optimal quantity and efficiency, and supporting the winegrower with decision-making. A Kinect v2 system on-board to an on-ground electric vehicle was capable of producing precise 3D point clouds of vine rows under six different management cropping systems. The generated models demonstrated strong consistency between 3D images and vine structures from the actual physical parameters when average values were calculated. Correlations of Kinect branch volume with pruning weight (dry biomass) resulted in high coefficients of determination (R² = 0.80). In the study of vineyard yield correlations, the measured volume was found to have a good power law relationship (R² = 0.87). However due to low capability of most depth cameras to properly build 3-D shapes of small details the results for each treatment when calculated separately were not consistent. Nonetheless, Kinect v2 has a tremendous potential as a 3D sensor in agricultural applications for proximal sensing operations, benefiting from its high frame rate, low price in comparison with other depth cameras, and high robustness. Full article

The optimisation of vineyards management requires efficient and automated methods able to identify individual plants. In the last few years, Unmanned Aerial Vehicles (UAVs) have become one of the main sources of remote sensing information for Precision Viticulture (PV) applications. In fact, high resolution UAV-based imagery offers a unique capability for modelling plant’s structure making possible the recognition of significant geometrical features in photogrammetric point clouds. Despite the proliferation of innovative technologies in viticulture, the identification of individual grapevines relies on image-based segmentation techniques. In that way, grapevine and non-grapevine features are separated and individual plants are estimated usually considering a fixed distance between them. In this study, an automatic method for grapevine trunk detection, using 3D point cloud data, is presented. The proposed method focuses on the recognition of key geometrical parameters to ensure the existence of every plant in the 3D model. The method was tested in different commercial vineyards and to push it to its limit a vineyard characterised by several missing plants along the vine rows, irregular distances between plants and occluded trunks by dense vegetation in some areas, was also used. The proposed method represents a disruption in relation to the state of the art, and is able to identify individual trunks, posts and missing plants based on the interpretation and analysis of a 3D point cloud. Moreover, a validation process was carried out allowing concluding that the method has a high performance, especially when it is applied to 3D point clouds generated in phases in which the leaves are not yet very dense (January to May). However, if correct flight parametrizations are set, the method remains effective throughout the entire vegetative cycle. Full article

With the advent of agriculture 3.0 and 4.0, in view of efficient and sustainable use of resources, researchers are increasingly focusing on the development of innovative smart farming and precision agriculture technologies by introducing automation and robotics into the agricultural processes. Autonomous agricultural field machines have been gaining significant attention from farmers and industries to reduce costs, human workload, and required resources. Nevertheless, achieving sufficient autonomous navigation capabilities requires the simultaneous cooperation of different processes; localization, mapping, and path planning are just some of the steps that aim at providing to the machine the right set of skills to operate in semi-structured and unstructured environments. In this context, this study presents a low-cost, power-efficient local motion planner for autonomous navigation in vineyards based only on an RGB-D camera, low range hardware, and a dual layer control algorithm. The first algorithm makes use of the disparity map and its depth representation to generate a proportional control for the robotic platform. Concurrently, a second back-up algorithm, based on representations learning and resilient to illumination variations, can take control of the machine in case of a momentaneous failure of the first block generating high-level motion primitives. Moreover, due to the double nature of the system, after initial training of the deep learning model with an initial dataset, the strict synergy between the two algorithms opens the possibility of exploiting new automatically labeled data, coming from the field, to extend the existing model’s knowledge. The machine learning algorithm has been trained and tested, using transfer learning, with acquired images during different field surveys in the North region of Italy and then optimized for on-device inference with model pruning and quantization. Finally, the overall system has been validated with a customized robot platform in the appropriate environment. Full article

Crop 3D modeling allows site-specific management at different crop stages. In recent years, light detection and ranging (LiDAR) sensors have been widely used for gathering information about plant architecture to extract biophysical parameters for decision-making programs. The study reconstructed vineyard crops using light detection and ranging (LiDAR) technology. Its accuracy and performance were assessed for vineyard crop characterization using distance measurements, aiming to obtain a 3D reconstruction. A LiDAR sensor was installed on-board a mobile platform equipped with an RTK-GNSS receiver for crop 2D scanning. The LiDAR system consisted of a 2D time-of-flight sensor, a gimbal connecting the device to the structure, and an RTK-GPS to record the sensor data position. The LiDAR sensor was facing downwards installed on-board an electric platform. It scans in planes perpendicular to the travel direction. Measurements of distance between the LiDAR and the vineyards had a high spatial resolution, providing high-density 3D point clouds. The 3D point cloud was obtained containing all the points where the laser beam impacted. The fusion of LiDAR impacts and the positions of each associated to the RTK-GPS allowed the creation of the 3D structure. Although point clouds were already filtered, discarding points out of the study area, the branch volume cannot be directly calculated, since it turns into a 3D solid cluster that encloses a volume. To obtain the 3D object surface, and therefore to be able to calculate the volume enclosed by this surface, a suitable alpha shape was generated as an outline that envelops the outer points of the point cloud. The 3D scenes were obtained during the winter season when only branches were present and defoliated. The models were used to extract information related to height and branch volume. These models might be used for automatic pruning or relating this parameter to evaluate the future yield at each location. The 3D map was correlated with ground truth, which was manually determined, pruning the remaining weight. The number of scans by LiDAR influenced the relationship with the actual biomass measurements and had a significant effect on the treatments. A positive linear fit was obtained for the comparison between actual dry biomass and LiDAR volume. The influence of individual treatments was of low significance. The results showed strong correlations with actual values of biomass and volume with R2 = 0.75, and when comparing LiDAR scans with weight, the R2 rose up to 0.85. The obtained values show that this LiDAR technique is also valid for branch reconstruction with great advantages over other types of non-contact ranging sensors, regarding a high sampling resolution and high sampling rates. Even narrow branches were properly detected, which demonstrates the accuracy of the system working on difficult scenarios such as defoliated crops. Full article