Chu 13

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CHU 13 medium is a culture medium used in microbiology for the growth of certain algal species, first published by S.P. Chu in 1942. [1] It is used as growth medium for the biofuel candidate alga Botryococcus braunii . [2]

Botryococcus braunii Botryococcus braunii.jpg
Botryococcus braunii

CHU 13 includes essential minerals and trace elements that are required by algae for growth, but does not include a carbon source and so is only appropriate for growth of phototrophs. It can be prepared as either a liquid medium or as an agar medium.

Modified CHU13 Medium [3]
Compoundmg/L
KNO3 400
K2HPO480
CaCl2 dihydrate 107
MgSO4 heptahydrate 200
Ferric citrate 20
Citric acid 100
CoCl2 0.02
H3BO3 5.72
MnCl2 tetrahydrate 3.62
ZnSO4 heptahydrate0.44
CuSO4 pentahydrate 0.16
Na2MoO4 0.084
0.072 N H2SO4 1 drop

The remaining volume is pure, de-ionized water.

Because it is difficult to weigh out some of the trace minerals, it is advisable to create a mixture of all components at a large concentration, such as a thousand times these measures, and then mix with the appropriate amount of (pure, de-ionized) water. Correct pH to 7.5, and then autoclave.

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References

  1. S.P. Chu (Aug., 1942) The Journal of Ecology, Vol. 30, No. 2, pp. 284-325. https://www.cpp.edu/~biodiesel/documents/reference/Chu42AlgaIsolGrowth.pdf
  2. C. Largeau, E. Casadevall, C. Berkaloff and P. Dhamelincout (1980) Phytochemistry, 19, 1943.
  3. K. Yamaguchi; et al. (1987). "Lipid composition of a green alga, Botryococcus braunii". Agric. Biol. Chem. 51 (2): 493–498. doi: 10.1271/bbb1961.51.493 .