Muscarinic Acetylcholine Receptor Agonists as Novel Treatments for Schizophrenia

The initial development of mAChR-based therapies for CNS disorders focused primarily on treating Alzheimer’s disease–associated cognitive impairment, which results, at least in part, from loss of cholinergic innervation from forebrain regions such as the nucleus basalis and longitudinal band projecting to the hippocampus, afferents important for cognition and memory (65, 66). This prompted drug developers to create direct-acting ACh-mimetic agonist drugs based on the structure of arecoline, a well-characterized ACh-mimetic agonist for mAChRs (41, 67, 68). The result was the development of a number of mAChR agonists, several of which were taken into clinical trials, such as RS-86, milameline, cevimeline, tazomeline, talsaclidine, alvameline, sabcomeline, and xanomeline, with the intent of stimulating postsynaptic M₁ receptors and thus circumventing the loss of ACh in Alzheimer’s disease (41, 69). Although these agents were developed as M₁ receptor–targeted compounds, in practice they displayed only modest pharmacological selectivity across all five mAChRs (70).

Some of these M₁ receptor–targeted compounds demonstrated detectable, although modest, improvements in cognition; however, parasympathetically mediated adverse events, including nausea and vomiting, increased gastrointestinal motility, salivation, and sweating, prevented their further clinical development (41, 71). For example, cevimeline initially yielded positive results on both the Alzheimer’s Disease Assessment Scale–Cognitive Subscale (ADAS-Cog) and caregiver impression scales (72). However, in a larger follow-up study, cevimeline subsequently failed to improve these same prespecified primary endpoints (73). Similarly, initial results from a clinical trial of sabcomeline in patients with Alzheimer’s disease indicated that it, too, improved ADAS-Cog scores (74), but it failed to meet its primary cognitive endpoint in a phase 3 trial (75). In a phase 2 study in patients with schizophrenia, sabcomeline was also reported to have no effect on Positive and Negative Syndrome Scale (PANSS) scores compared with placebo; however, trends in improved cognitive function were reported (76).

Overall, the often-cited reasons for discontinuation of these first-generation full or partial M₁ receptor–targeted orthosteric agonists were the lack of efficacy and/or poor tolerability. The trials did not include any CNS assessment of activity, such as CNS muscarinic receptor occupancy, which is not surprising given the lack of reliable positron emission tomography ligands when these trials were conducted. Thus, it is difficult to conclude whether these trials failed owing to suboptimal dosing caused by dose-limiting peripheral mAChR adverse events or a lack of therapeutic activity for any given compound. At the time, the poor tolerability of these compounds was believed to be primarily due to their M₂ and M₃ receptor activity (48), but further work (see below) with mAChR subtype–selective ligands suggested that M₁ and M₄ receptors also likely played a role in the peripherally mediated adverse events observed with this first generation of mAChR-targeted drug candidates.

Although initially developed to specifically treat the cognitive symptoms of Alzheimer’s disease, the mAChR agonist xanomeline, as briefly discussed above, significantly reduced and prevented the emergence of behavioral, including psychotic, disturbances in patients with Alzheimer’s disease and resulted in modestly improved cognitive impairment as measured by the ADAS-Cog (22). These promising clinical data in patients with Alzheimer’s disease encouraged a considerable number of preclinical studies across academia and industry (discussed in detail below) to substantiate the neurobiological underpinnings of these surprising clinical results. Moreover, these findings also prompted a small (N=20), proof-of-concept, phase 2 follow-up trial in acutely psychotic patients with chronic schizophrenia (23). In that trial, when compared with placebo, treatment with xanomeline resulted in significant improvements in total Brief Psychiatric Rating Scale and PANSS scores as well as improvement in several cognitive domains, specifically in measures of verbal learning and short-term memory function. These rather compelling results obtained in patients with either Alzheimer’s disease (22) or schizophrenia (23) stimulated further research on the potential utility of mAChR agonists for treating a variety of neuropsychiatric disorders. Also evident from these early studies, however, was the need for improved tolerability, primarily with respect to limiting peripheral mAChR stimulation.

Following these early encouraging clinical data with xanomeline, the field set out to develop “mAChR-targeted” agents that would maintain the clinical benefits associated with xanomeline while reducing the associated mAChR-mediated adverse events. To accomplish this, two different approaches have been pursued. The predominant approach has been to develop compounds that more selectively target M₁ and/or M₄ receptors, the two mAChRs believed to be most associated with the benefits of xanomeline, via their allosteric (63) rather than orthosteric binding sites.

The other approach is a strategy recently adopted for reducing the peripheral side effects of xanomeline while maintaining its therapeutic benefits in the CNS: coadministration of xanomeline with a peripherally restricted mAChR antagonist (77). By blocking peripheral mAChRs with trospium (a U.S. Food and Drug Administration–approved, non–brain-penetrant, pan-mAChR antagonist) (78) while simultaneously activating central mAChRs with xanomeline, tolerability was markedly improved while maintaining xanomeline’s centrally mediated therapeutic benefits. The high brain-to-plasma ratio (>10:1) of xanomeline also likely favors CNS-mediated therapeutic activity observed across these clinical trials (79–81).

The results from a recently completed placebo-controlled phase 2 trial in patients with schizophrenia experiencing acute psychotic symptoms (82) demonstrated that treatment with the investigational drug combination of xanomeline and trospium (KarXT) resulted in a significant reduction in PANSS total score as well as a number of secondary endpoints (e.g., PANSS positive subscale, PANSS negative subscale, and Clinical Global Impressions severity scale) compared with placebo (Figure 4). Additionally, an exploratory post hoc analysis (83) suggests that KarXT treatment was also associated with greater improvement in cognitive performance compared with placebo in patients who were cognitively impaired at baseline. However, additional prospective studies will be needed to confirm the potential procognitive benefits of KarXT. KarXT was associated with mild to moderate cholinergic and anticholinergic adverse events that were generally transient and did not lead to discontinuation from the trial. Treatment with KarXT was not associated with extrapyramidal side effects, weight gain, or sedation (82). Although these phase 2 data are encouraging, phase 3 studies will be required to confirm the efficacy and safety profile observed in phase 2. Several phase 3 studies are currently under way (NCT04659161, NCT04738123, NCT04659174, NCT04820309, NCT05145413).

These results, which replicate the earlier phase 2 trials with xanomeline (22, 23), strongly suggest that mAChRs are compelling drug targets to potentially modulate neural circuits disrupted in patients with schizophrenia. Still to be explained, however, is whether one or more mAChR subtypes contribute to xanomeline’s antipsychotic and procognitive activity and how mAChR agonists may impact neural circuits likely responsible for their potential benefits in treating the core symptoms of schizophrenia.

The onset of psychotic (i.e., positive) symptoms is believed to be associated with neural network dysfunction that includes a variety of brain regions and neurotransmitters, including increased activity of ventral tegmental area (VTA) DA neurons and heightened terminal DA release (104). Although the exact mechanism of how antipsychotic drugs achieve their therapeutic activity is still not completely understood, it has been postulated, with considerable experimental support, that most, if not all, antipsychotic drugs induce “depolarization block” or inactivation of DA neurons in both the VTA (“mesolimbic DA circuit”) and the substantia nigra (SN; “motor DA circuit”); this phenomenon takes place over a period of time that parallels the delay in the onset of clinical improvement (105). Based on the “depolarization block” hypothesis, antipsychotic drugs with more selective actions on VTA DA neurons consistently show a lower motor symptom liability (i.e., low extrapyramidal side effects), whereas high liability to extrapyramidal side effects is associated with antipsychotic drugs that more markedly reduce the activity of DA neurons in the SN, which is involved in the control of the extrapyramidal motor control system (106, 107). Importantly and often overlooked is that the activity of midbrain VTA DA neurons is modulated by cholinergic input from hindbrain structures (108), particularly the laterodorsal tegmental nucleus (LDT), where M₄ receptors are abundantly expressed and function as autoreceptors (49, 109). M₄ receptors play a key role in blunting the stimulatory effects of ACh on DA neurons, making them less active and thereby reducing downstream DA release in brain regions implicated in psychosis, such as the nucleus accumbens and ventral striatum (Figure 5A) (49, 109).

Interestingly, xanomeline has been shown to selectively and rapidly reduce VTA DA neuron firing rates but not SN DA neuron activity (24), an effect now believed to be mediated by selective activation of LDT afferents (110, 111). This very rapid reduction in VTA DA neuron activity observed following acute administration of xanomeline (24) contrasts with the slow, time-dependent depolarization block of these same DA neurons that occurs following treatment with second-generation antipsychotics (105, 112, 113). The DA D₂ receptor–independent modulation of DA microcircuits may also explain the relatively rapid antipsychotic effects of xanomeline reported in patients with Alzheimer’s disease (22) or schizophrenia (23). Also consistent with xanomeline’s selective action on mesolimbic VTA DA neurons is the lack of overt motor effects (e.g., catalepsy) observed in preclinical models (24, 90) or its lack of effect in inducing immediate early gene expression (e.g., cFos) in brain regions associated with extrapyramidal side effects (114). This highly selective regulation of DA-containing neural circuits seen with xanomeline may therefore account for the lack of observed extrapyramidal side effects in multiple clinical trials (22, 23, 82) and a much lower, if not absent, risk of developing tardive dyskinesia.

In addition to their ability to regulate midbrain VTA DA neuron activity, M₄ receptors are expressed in cholinergic interneurons that reside locally in the nucleus accumbens and modulate terminal DA release (115, 116). Within the nucleus accumbens, activation of M₄ autoreceptors on cholinergic interneurons decreases spontaneous activity, which reduces ACh release to dampen nicotinic receptor feed-forward stimulation on DA terminals (Figure 5B) (116, 117). It should be noted, however, that there are other postulated mechanisms by which M₄ receptor–mediated inhibition of local DA release may occur, such as activation of M₄ receptors coexpressed in D₁ receptor–expressing medium spiny neurons (MSNs) of the dorsolateral striatum (118, 119). In support of this hypothesis, selective deletion of M₄ receptors from D₁ MSNs increases DA-dependent behavioral phenotypes (119) and blunts M₄ receptor–mediated inhibition of DA release in preclinical models (120), an effect likely mediated through competition of convergent second messenger systems (56), recruitment of endocannabinoids (120), interactions with G_q-coupled receptors (121), or direct actions on enhanced Ca²⁺ currents via Ca_v1 channels (122). Genetically eliminating M₄ receptors from D₁ MSNs also markedly reduces the inhibitory effects of xanomeline on amphetamine-stimulated locomotor activity (118).

More recent studies using highly selective M₄ receptor PAMs support the important role that these receptors play in mediating antipsychotic drug–like behavioral activity, as numerous chemical scaffolds that modulate M₄ receptors display antipsychotic activity (60, 120, 123–125). In addition to modulating classical neural circuits implicated in psychosis, M₄ receptor PAMs have been shown to enhance attentional and memory network function in preclinical rodent models (126). M₄ receptor agonists can reduce elevated CA1 pyramidal neuron activity, which may partially contribute to their procognitive effects (85), as changes in CA1 excitability have been postulated to contribute to the cognitive deficits in schizophrenia (27). However, additional detailed studies are needed to fully understand the multinodal mechanisms by which M₄ receptors impact local subcortical microcircuits within the dorsal (i.e., associative striatum) and ventral striatum to inhibit DA neurotransmission (for a review, see reference 9).

Although M₄ receptor regulation of DA microcircuits appears to be important in the central mechanism of action of xanomeline, glutamatergic microcircuits may also contribute to its antipsychotic activity. In rodents, acute administration of NMDA receptor antagonists, such as PCP or MK-801, produces behavioral hyperactivity and cognitive deficits that correlate with a disinhibition of pyramidal cell firing in the prefrontal cortex and increased DA and glutamate levels (127, 128). Administration of xanomeline or a selective M₄ receptor PAM can attenuate PCP- or MK-801-evoked locomotor activity, an effect that is absent in global M₄ receptor knockout mice (123). M₄ receptor–mediated regulation of MK-801-induced hyperactivity may involve actions at corticostriatal terminals to normalize the function of overactive excitatory glutamatergic projections to the striatum (129) or via a dampening of thalamocortical synapses (130). This “top-down” control of cortical glutamatergic projections has important implications, as these projections indirectly modulate phasic DA release (118). In addition, activation of M₄ receptors attenuates MK-801-induced disruptions in learning and memory (123) and elevations of high-frequency gamma power as well as state-dependent alterations in sleep architecture and arousal similar to the effects observed with atypical antipsychotics in preclinical models (131). Taken together, these findings suggest an important role for M₄ receptors in modulating neural circuits involved in the psychotic, motivational, cognitive, and executive functions disrupted in schizophrenia via glutamatergic microcircuits.

These behavioral studies describing an important role for M₄ receptors in animal models of psychosis have led to further studies exploring the potential role of M₄ receptors in the pathophysiology of schizophrenia. In postmortem studies, M₄ receptor expression has been reported to be decreased in striatal and hippocampal brain regions of patients with schizophrenia compared with those of healthy control subjects (132). Other studies using modestly selective mAChR antagonist radioligands also suggest that M₄ receptors are decreased in the frontal cortex, hippocampus, and striatum of patients with schizophrenia compared with those of control subjects (133, 134). In tandem with the apparent decreases in M₄ receptor expression, these morphometric changes could also contribute to decreased regional brain volumes and cortical thickness. Genetic markers (single-nucleotide polymorphisms) in a region on chromosome 11 have been reported to be associated with schizophrenia (135), and this region contains several candidate genes, including CHRM4, the gene encoding the M₄ receptor. Two genomic variants of the M₄ receptor, rs2067482 and rs72910092, have also been reported to be associated with an increased risk of schizophrenia (136). It should be noted, however, that these allelic associations have not been consistently replicated across studies (137), which may be due to differences in the patient populations sampled and diagnostic subtyping.

Importantly, recent clinical results were released from a phase 1b placebo-controlled trial of emraclidine (CVL-231), an M₄ receptor PAM, in patients with schizophrenia (138). In an exploratory analysis, treatment with CVL-231 was associated with reduced psychotic symptoms compared with placebo after 6 weeks of treatment (138). Rates of gastrointestinal adverse events were minimal compared to those in historical mAChR agonist trials (41, 69). These data provide additional evidence that M₄ receptors play key roles in mediating the antipsychotic properties of mAChR agonists (138) and preliminary clinical validation for drugs that target allosteric binding pockets of mAChR receptors (in this case M₄ receptors). Although encouraging, these data have yet to be peer reviewed, and further placebo-controlled trials of CVL-231 will be necessary to establish its efficacy and safety profile in patients with schizophrenia.

Although an important role for M₄ receptors in mediating the antipsychotic effects of xanomeline is quite likely (see above) there is also substantial evidence that M₁ receptors regulate neural circuits underlying psychosis as well as learning and memory (especially working memory). Xanomeline’s “antipsychotic” activity as measured by amphetamine-induced hyperactivity was abolished in mice lacking M₄ receptors but also was partially attenuated in mice lacking M₁ receptors, suggesting that M₄ receptors and, to a certain degree, M₁ receptors may both contribute to xanomeline’s efficacy in treating the positive symptoms of schizophrenia (97). As xanomeline is a “dual” M₁ and M₄ receptor agonist, M₁ receptors may also contribute to its reported antipsychotic and procognitive activity.

Several hypotheses have emerged regarding how M₁ receptors may modulate neural circuits implicated in psychosis, including regulation of top-down circuits that synapse onto VTA DA neurons (Figure 6) (139, 140). For example, activation of M₁ receptors facilitates excitability of cortical GABAergic interneurons that synapse onto pyramidal neurons (139), causing a decrease in excitability of principal cortical output neurons. However, there are additional hypotheses regarding how M₁ receptor activators may exhibit antipsychotic activity, including augmentation of corticostriatal plasticity (141), modulation of MSN excitability (142), and enhanced communication between MSNs (e.g., via nucleus accumbens output neurons) (143). Additional studies have demonstrated a “psychosis-like” phenotype in global M₁ receptor knockout mice (144) as well as antipsychotic drug–like activities in various preclinical models following administration of selective M₁ receptor PAMs (145, 146). M₁ receptor PAMs have also been reported to reverse excessive spontaneous locomotor activity in NMDA receptor NR1-subunit knockdown mice that display an NMDA receptor–mediated hypofunction phenotype (147).

As summarized above, significant effort has been made to develop M₁ receptor agonists to treat the cognitive impairment associated with various neuropsychiatric and neurodegenerative disorders (64, 70). In memory circuits, M₁ receptor activators have been shown to enhance synaptic plasticity (85, 148), increase neuronal excitability (149), and facilitate learning and improve cognition in aged animals (150) and in a variety of NMDA receptor hypofunction models of impaired learning and memory (147, 149, 151, 152). Previous studies have shown that M₁ receptors are physically and functionally coupled to NMDA receptors and that activation of M₁ receptors potentiates NMDA receptor currents in cortical and hippocampal pyramidal neurons (54, 153, 154). Conversely, global M₁ receptor knockout mice have impaired performance in prefrontal cortex–dependent cognitive tasks (155, 156) and reduced hippocampal long-term potentiation (157). Administration of an M₁ receptor potentiator in genetic models of NMDA receptor hypofunction restored plasticity deficits and improved impaired learning and memory in these mice (147). Moreover, M₁ receptor PAMs have been shown to modulate sleep-wake architecture in rodents and nonhuman primates (150), suggesting that activation of M₁ receptors may participate in restorative sleep–mediated plasticity, which has been postulated to be dysregulated in patients with schizophrenia (158). Beyond cognition, M₁ receptor PAMs have been reported to reverse behavioral and electrophysiological deficits in chronic PCP rodent models, which are thought to recapitulate the “deficit state” or negative symptoms of schizophrenia (149). Recently it was shown that M₁ receptor PAMs can reverse PCP-induced disruption of mAChR-stimulated long-term depression (149), a plasticity measure that is important for adapting neural networks to physiological activity. These studies support the idea that M₁ receptor activation may, by itself, contribute to improvements in the cognitive, negative, and even positive symptom domains of schizophrenia, and thus a dual M₁/M₄-preferring mAChR agonist may be particularly effective in treating schizophrenia.

As mentioned earlier, clozapine appears to be unique among the drugs used to treat schizophrenia, as it appears to be more effective in treating positive and negative symptoms in patients with treatment-resistant illness (159). Moreover, some (160) but not all (161) studies have reported that clozapine can improve cognition and especially working memory performance in patients with schizophrenia. Interestingly, although clozapine itself is a potent mAChR antagonist (162), its major metabolite, N-desmethylclozapine (NDMC), is a potent partial M₁ receptor agonist (163). Recently, it has been reported by several groups that lower clozapine-to-NDMC ratios are associated with improvements in working memory and executive function, whereas higher ratios are associated with cognitive deficits (164–167). These findings raise the intriguing possibility that the M₁ receptor activity of NDMC contributes to clozapine’s unique clinical profile, including its reported procognitive benefits (160). Several lines of evidence suggest that M₁ receptors may also play a key role in the ability of clozapine to modulate schizophrenia-related circuity in rodents (168–170). Sub-efficacious doses of clozapine can reverse MK-801-induced deficits in sensorimotor gating, and this is potentiated by coadministration of an M₁ receptor PAM (151). From a circuit-level perspective, local administration of NDMC can alter DA release in brain regions implicated in psychosis, an effect that is opposite to that of clozapine (171).

However, in preclinical models (172), and in marked contrast to clozapine, NDMC did not display antipsychotic activity, and a subsequent phase 2 clinical trial in patients with schizophrenia confirmed its lack of efficacy in treating positive symptoms (173). Although NDMC shares many of clozapine’s pharmacological properties beyond mAChR activity, it does not occupy or block DA D₂ receptors (174); this may account for its lack of efficacy in treating positive symptoms. However, it is quite possible that NDMC accounts for the procognitive effects of clozapine and possibly its beneficial effects on negative symptoms. NDMC could also be responsible for some of the peripheral adverse effects observed with clozapine (175), such as hypersalivation. Moreover, the peripheral gastrointestinal adverse events reported for xanomeline are almost certainly due to stimulation of peripheral mAChRs. Nonetheless, these data fit with the hypothesis that M₁ receptor activation could be a key mechanism through which clozapine exerts its unique clinical profile (175).

Previous studies have also suggested a role for M₁ receptors in the pathophysiology of schizophrenia. For instance, using [¹²³I]quinuclidinyl benzilate, mAChR availability was found to be reduced in unmedicated patients with schizophrenia (21), a finding similar to that in the postmortem studies. Several postmortem studies using the mAChR antagonist [³H]-pirenzepine have demonstrated decreased M₁ receptor expression in cortical and subcortical regions in patients with schizophrenia (62, 176, 177). The authors refer to these patients as having “mAChR deficit” schizophrenia. Interestingly, whereas there is a pronounced loss of M₁ receptors in the mAChR deficit schizophrenia subtype, the residual M₁ population has increased receptor–G protein coupling efficiency, suggesting an adaptive change to compensate for reduced receptor expression (178). Importantly, these changes in M₁ receptor expression appear to be specific to schizophrenia and may represent a distinguishable endophenotype (177). Additional evidence that M₁ receptors may contribute to the pathophysiology of schizophrenia include recent reports that homozygous carriers of CHRM1 C267A nucleotide polymorphisms exhibit pronounced perseveration errors and poor performance on tests of executive functioning (179, 180). More recently, elevated serum titers of anti–M₁ receptor antibodies have been reported in up to one-third of people diagnosed with schizophrenia (181, 182), and their presence was correlated with the severity of negative symptoms (181).

In actual practice, it is likely that many patients with schizophrenia will be treated simultaneously with conventional antipsychotic drugs and novel therapies to improve overall efficacy before they are switched to monotherapy with a novel drug (183). This concept is supported by preclinical data showing that treatment with the M₁ PAM BQCA in combination with atypical antipsychotics (i.e., aripiprazole and clozapine) provided synergistic procognitive activity in deficit states induced by the NMDA receptor antagonist MK-801 (151). Although additional studies are needed to fully understand the underlying neural circuits involved in mediating the procognitive effects of BQCA, they likely involve modulation of hippocampal synaptic plasticity (184, 185).

In the context of psychosis, subeffective doses of the M₄ PAM PGM039678 in combination with subeffective doses of the atypical antipsychotics olanzapine or risperidone significantly augmented conditioned avoidance responding in rats (186). Recently, it was also reported that subeffective doses of xanomeline augmented the activity of aripiprazole and risperidone in the conditioned avoidance response assay in mice (103). In the same study, subthreshold doses of xanomeline and risperidone administered together significantly attenuated MK-801-induced hyperactivity (103). These results raise the intriguing possibility that M₁ receptor, M₄ receptor, or dual M₁/M₄ receptor agonists may represent adjunctive treatments, when used together with currently prescribed first- or second-generation antipsychotics, to improve the core symptoms of schizophrenia, especially in patients with treatment-resistant illness.

Pharmacological studies using M₂ receptor–preferring antagonists have produced contradictory results regarding their potential role in cognition. Whereas some studies suggest that blockade of central M₂ receptors enhances learning and memory in various experimental settings (187), other studies arrived at the opposite conclusion (188). M₂ receptor global knockout mice display deficits in behavioral flexibility, working memory, and passive avoidance learning (189). However, M₂ receptors are apparently not required for stimulus-reward learning. Reduced expression of subcortical (190, 191) but not cortical (192) M₂ receptors has also been found in patients with schizophrenia. In preclinical models, the antipsychotic-like activity of BuTAC, an M₂ receptor–preferring orthosteric agonist and a partial M₁/M₄ receptor agonist, is lost in global M₂/M₄ double-knockout mice (193). From a microcircuit perspective, there appears to be a functional interplay between M₂ and M₄ receptors to regulate DA release in striatal regions (116), suggesting that M₂ receptor activation may play an important role in M₄-mediated suppression of DA release. Additional studies are needed to understand the therapeutic potential of dual M₂-M₄ receptor agonists or whether M₂-M₄ heterodimers play a role in psychosis.

The M₃ receptor is expressed widely in the CNS, including in the hippocampus (194), and may play an important role in learning and memory. M₃ receptor knockout mice have severe deficits in hippocampus-dependent memory, suggesting that selective M₃ receptor activators may be beneficial for cognition (195). Recently, it has been reported that M₃ receptors regulate feed-forward inhibition, which may facilitate memory consolidation by reducing interference signals (196). Detailed biochemical studies support an important role for M₃ receptor signaling through phosphorylation events as contributing to these procognitive effects (195) and raise the possibility that M₃ receptor–biased ligands that increase β-arrestin-dependent (non–G protein) signaling might promote cholinergic-mediated learning and memory (197).

Finally, M₅ receptors may also be a promising drug target for schizophrenia given their expression in and control of midbrain DA neurons (198). M₅ receptor global knockout mice display reduced striatal DA release and blunted responses in preclinical models of psychosis (199). Activation of M₅ receptors increases the activity of midbrain DA neurons (200) to facilitate terminal DA release (201), suggesting that therapeutic agents that inhibit the M₅ receptor will decrease elevated DA levels reported in various forebrain regions in schizophrenia. More recent preclinical evidence suggests that M₅ receptor agonists or PAMs may also be effective for treating negative symptoms (202) and cognitive deficits (203). Continued efforts are needed to develop CNS-penetrant compounds with appreciable M₅ receptor agonist or antagonist selectivity to further elucidate their potential benefits (204, 205). In this regard, xanomeline has been shown to be an M₅ receptor partial agonist (206) and thus is likely to have a predominant M₅ receptor antagonist pharmacological profile (206). The contribution, if any, of xanomeline’s M₅ receptor activity, along with its M₁ and M₄ receptor pharmacology, to its reported clinical (antipsychotic and procognitive) activity in patients with schizophrenia is unclear but cannot be excluded.