Kurtz et al., 1981 - Google Patents

Cloning of α2u globulin cDNA using a high efficiency technique for the cloning of trace messenger RNAs

Kurtz et al., 1981

Document ID
7611516796910945622
Author
Kurtz D
Nicodemus C
Publication year
Publication venue
Gene

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Snippet

An extremely high-efficiency technique is described for cloning double-stranded (ds) cDNAs in Escherichia coli. The method which uses two synthetic oligonucleotide linkers rather than one results in approx. 200–500 recombinant clones per ng of ds cDNA. This technique was …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
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