Gill, 2023 - Google Patents

A study of viral entry proteins and receptors using deep mutagenesis

Gill, 2023

View PDF
Document ID
18089880528419840378
Author
Gill K
Publication year

External Links

Snippet

Mutagenesis allows for the analysis and manipulation of protein interactions and can be especially insightful when protein complexes are challenging for structure determination. Both targeted and random mutagenesis have their benefits but also have limitations …
Continue reading at www.ideals.illinois.edu (PDF) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses, e.g. hepatitis E virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse Transcribing RNA Viruses
    • C12N2740/00011—Reverse Transcribing RNA Viruses
    • C12N2740/10011—Retroviridae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay
    • G01N33/569—Immunoassay; Biospecific binding assay for micro-organisms, e.g. protozoa, bacteria, viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans

Similar Documents

Publication Publication Date Title
Procko The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2
Nelson et al. Structure and intracellular targeting of the SARS-coronavirus Orf7a accessory protein
Sauter et al. Human tetherin exerts strong selection pressure on the HIV-1 group N Vpu protein
He et al. Targeted isolation of diverse human protective broadly neutralizing antibodies against SARS-like viruses
Serra-Moreno et al. Tetherin/BST-2 antagonism by Nef depends on a direct physical interaction between Nef and tetherin, and on clathrin-mediated endocytosis
Maerz et al. Functional analysis of the disulfide-bonded loop/chain reversal region of human immunodeficiency virus type 1 gp41 reveals a critical role in gp120-gp41 association
WO2016037154A1 (en) Recombinant hiv-1 envelope proteins and their use
Gao et al. Designing a soluble near full-length HIV-1 gp41 trimer
US11267874B2 (en) Sensor systems for target ligands and uses thereof
Heredia et al. Conformational engineering of HIV-1 Env based on mutational tolerance in the CD4 and PG16 bound states
CA2578253A1 (en) Reverse transfection of cell arrays
Guan et al. Residues 28 to 39 of the extracellular loop 1 of chicken Na+/H+ exchanger type I mediate cell binding and entry of subgroup J avian leukosis virus
Ye et al. Oligomerization is required for HIV-1 Nef-induced activation of the Src family protein-tyrosine kinase, Hck
Barnett et al. Structure and mechanism of a coreceptor for infection by a pathogenic feline retrovirus
Groß et al. Mimicking protein–protein interactions through peptide–peptide interactions: HIV-1 gp120 and CXCR4
CA3140064A1 (en) Lockr-mediated recruitment of car t cells
Lu et al. Illuminating the virus life cycle with single-molecule FRET imaging
KR20210097833A (en) Novel mammalian expressed human immunodeficiency virus envelope protein antigens
Masuda et al. Analysis of receptor usage by ecotropic murine retroviruses, using green fluorescent protein-tagged cationic amino acid transporters
KR20210035806A (en) Solubilized apyrase, methods and uses
Gill A study of viral entry proteins and receptors using deep mutagenesis
DK1981904T3 (en) Peptide domain from the enclosure of a HERV-W virus, which is required for reaction with a hASCT receptor
Jin et al. GLUT-1-independent infection of the glioblastoma/astroglioma U87 cells by the human T cell leukemia virus type 1
Raney et al. Structural constraints on human immunodeficiency virus type 1 Nef function
US20230174592A1 (en) Detection reagent for screening blocking agent of coronavirus infections, and detection method