WO2005103230A3 - Soy peptone as a nitrogen source in preparing meningococcal conjugates - Google Patents

Soy peptone as a nitrogen source in preparing meningococcal conjugates Download PDF

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Publication number
WO2005103230A3
WO2005103230A3 PCT/GB2005/001543 GB2005001543W WO2005103230A3 WO 2005103230 A3 WO2005103230 A3 WO 2005103230A3 GB 2005001543 W GB2005001543 W GB 2005001543W WO 2005103230 A3 WO2005103230 A3 WO 2005103230A3
Authority
WO
WIPO (PCT)
Prior art keywords
preparing
nitrogen source
soy peptone
bacterium
meningococcal conjugates
Prior art date
Application number
PCT/GB2005/001543
Other languages
French (fr)
Other versions
WO2005103230A2 (en
Inventor
Cameron John Marshall
Original Assignee
Chiron Srl
Cameron John Marshall
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Srl, Cameron John Marshall filed Critical Chiron Srl
Priority to US11/587,174 priority Critical patent/US20080160044A1/en
Priority to EP05736004.2A priority patent/EP1756265B1/en
Publication of WO2005103230A2 publication Critical patent/WO2005103230A2/en
Publication of WO2005103230A3 publication Critical patent/WO2005103230A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/646—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent the entire peptide or protein drug conjugate elicits an immune response, e.g. conjugate vaccines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method for preparing a protein-saccharide conjugate, comprising the steps of: (a) preparing an aqueous growth medium comprising soy peptone as a nitrogen source; (b) inoculating the medium with a Neisseria meningitidis bacterium; (c) incubating the medium to allow growth of the bacterium; (d) preparing capsular saccharide from the bacterium; and (e) conjugating the capsular saccharide to a carrier protein, to give the a protein-saccharide conjugate. The conjugates are useful in vaccine production.
PCT/GB2005/001543 2004-04-22 2005-04-22 Soy peptone as a nitrogen source in preparing meningococcal conjugates WO2005103230A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/587,174 US20080160044A1 (en) 2004-04-22 2005-04-22 Soy Peptone as a Nitrogen Source in Preparing Meningcoccal Conjugates
EP05736004.2A EP1756265B1 (en) 2004-04-22 2005-04-22 Soy peptone as a nitrogen source in preparing meningococcal conjugates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0408978.5 2004-04-22
GBGB0408978.5A GB0408978D0 (en) 2004-04-22 2004-04-22 Meningococcal fermentation for preparing conjugate vaccines

Publications (2)

Publication Number Publication Date
WO2005103230A2 WO2005103230A2 (en) 2005-11-03
WO2005103230A3 true WO2005103230A3 (en) 2006-01-12

Family

ID=32344211

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/001543 WO2005103230A2 (en) 2004-04-22 2005-04-22 Soy peptone as a nitrogen source in preparing meningococcal conjugates

Country Status (4)

Country Link
US (1) US20080160044A1 (en)
EP (1) EP1756265B1 (en)
GB (1) GB0408978D0 (en)
WO (1) WO2005103230A2 (en)

Families Citing this family (21)

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MX339524B (en) 2001-10-11 2016-05-30 Wyeth Corp Novel immunogenic compositions for the prevention and treatment of meningococcal disease.
GB0413868D0 (en) * 2004-06-21 2004-07-21 Chiron Srl Dimensional anlaysis of saccharide conjugates
GB0605757D0 (en) * 2006-03-22 2006-05-03 Chiron Srl Separation of conjugated and unconjugated components
AR064642A1 (en) 2006-12-22 2009-04-15 Wyeth Corp POLINUCLEOTIDE VECTOR THAT INCLUDES IT RECOMBINATING CELL THAT UNDERSTANDS THE VECTOR POLYPEPTIDE, ANTIBODY, COMPOSITION THAT UNDERSTANDS THE POLINUCLEOTIDE, VECTOR, RECOMBINATING CELL POLYPEPTIDE OR ANTIBODY, USE OF THE COMPOSITION AND A COMPOSITION AND A METHOD
CN101597582B (en) * 2009-07-10 2011-04-06 中国医学科学院医学生物学研究所 Culture medium of meningitis Neisseria
TWI451874B (en) * 2010-05-03 2014-09-11 Dev Center Biotechnology Conjugates of polysaccharide and e. coli heat-labile toxin, their use and preparation method
CA2808975C (en) 2010-08-23 2018-10-30 Wyeth Llc Stable formulations of neisseria meningitidis rlp2086 antigens
MX362802B (en) 2010-09-10 2019-02-13 Wyeth Llc Star Non-lipidated variants of neisseria meningitidis orf2086 antigens.
RU2636350C2 (en) 2011-11-07 2017-11-22 Новартис Аг MOLECULE CONTAINING spr0096 AND spr2021
JP5925342B2 (en) 2012-03-09 2016-05-25 ファイザー・インク Neisseria meningitidis composition and method thereof
SA115360586B1 (en) 2012-03-09 2017-04-12 فايزر انك Neisseria meningitidis compositions and methods thereof
CN104487086B (en) * 2012-07-07 2019-08-30 巴拉特生物技术国际有限公司 Non-animal derived nonalcoholic vaccine composition and preparation method thereof
SG11201503947YA (en) * 2012-11-21 2015-06-29 Serum Inst India Ltd Production of high yields of bacterial polysaccharides
JP6446377B2 (en) 2013-03-08 2018-12-26 ファイザー・インク Immunogenic fusion polypeptide
KR20210002757A (en) 2013-09-08 2021-01-08 화이자 인코포레이티드 Neisseria meningitidis compositions and methods thereof
KR20190049940A (en) 2015-02-19 2019-05-09 화이자 인코포레이티드 Neisseria meningitidis compositions and methods thereof
KR102706358B1 (en) 2017-01-31 2024-09-11 화이자 인코포레이티드 Neisseria meningitidis compositions and methods thereof
CN108690816A (en) * 2017-04-12 2018-10-23 成都生物制品研究所有限责任公司 A kind of the non-animal source culture medium and its cultural method of A groups of neisseria meningitis inflammation coccus
KR20200007903A (en) * 2017-05-17 2020-01-22 엠에스디 웰컴 트러스트 힐레맨 랩스 피브이티. 리미티드 Production of Bacterial Polysaccharides
WO2019138432A1 (en) * 2018-01-15 2019-07-18 Msd Wellcome Trust Hilleman Laboratories Pvt. Ltd. Media composition for production of bacterial polysaccharides
CN117969862A (en) * 2024-04-02 2024-05-03 中国农业科学院农产品加工研究所 Screening method and application of soybean protein as microbial culture nitrogen source

Citations (8)

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Publication number Priority date Publication date Assignee Title
US4351761A (en) * 1978-05-15 1982-09-28 Research Corporation Purified antigen to test for Neisseria gonorrhoeae antibodies
US4814276A (en) * 1986-04-25 1989-03-21 Becton, Dickinson And Company Selective medium for growth of neisseria
EP0528635A1 (en) * 1991-08-16 1993-02-24 Merck & Co. Inc. Process for converting lipid-containing polysaccharide into lipid-free polysaccharide
US5494808A (en) * 1994-09-15 1996-02-27 Merck & Co., Inc. Defined medium OMPC fermentation process
WO1998054296A1 (en) * 1997-05-28 1998-12-03 Chiron S.P.A. Culture medium with yeast or soy bean extract as aminoacid source and no protein complexes of animal origin
WO2002058737A2 (en) * 2001-01-23 2002-08-01 Aventis Pasteur Multivalent meningococcal polysaccharide-protein conjugate vaccine
WO2003007985A2 (en) * 2001-06-20 2003-01-30 Chiron Srl. Capsular polysaccharide solubilisation and combination vaccines
WO2004033623A2 (en) * 2002-05-16 2004-04-22 Aventis Pasteur, Inc. Animal component free meningococcal polysaccharide fermentation and seedbank development

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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US4814276A (en) * 1986-04-25 1989-03-21 Becton, Dickinson And Company Selective medium for growth of neisseria
EP0528635A1 (en) * 1991-08-16 1993-02-24 Merck & Co. Inc. Process for converting lipid-containing polysaccharide into lipid-free polysaccharide
US5494808A (en) * 1994-09-15 1996-02-27 Merck & Co., Inc. Defined medium OMPC fermentation process
WO1998054296A1 (en) * 1997-05-28 1998-12-03 Chiron S.P.A. Culture medium with yeast or soy bean extract as aminoacid source and no protein complexes of animal origin
WO2002058737A2 (en) * 2001-01-23 2002-08-01 Aventis Pasteur Multivalent meningococcal polysaccharide-protein conjugate vaccine
WO2003007985A2 (en) * 2001-06-20 2003-01-30 Chiron Srl. Capsular polysaccharide solubilisation and combination vaccines
WO2004033623A2 (en) * 2002-05-16 2004-04-22 Aventis Pasteur, Inc. Animal component free meningococcal polysaccharide fermentation and seedbank development

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* Cited by examiner, † Cited by third party
Title
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MORIE G ET AL: "Development of a fermentation medium free from animal derived components for the production of polysaccharides from Neisseria meningitidis Group A and Group C", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 97, 4 May 1997 (1997-05-04), pages 183, XP008055404, ISSN: 1060-2011 *
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Also Published As

Publication number Publication date
EP1756265A2 (en) 2007-02-28
US20080160044A1 (en) 2008-07-03
EP1756265B1 (en) 2014-01-22
GB0408978D0 (en) 2004-05-26
WO2005103230A2 (en) 2005-11-03

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