WO2000034442A3 - Application of shear flow stress to smooth muscle cells for the production of implantable structures - Google Patents

Application of shear flow stress to smooth muscle cells for the production of implantable structures Download PDF

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Publication number
WO2000034442A3
WO2000034442A3 PCT/US1999/029257 US9929257W WO0034442A3 WO 2000034442 A3 WO2000034442 A3 WO 2000034442A3 US 9929257 W US9929257 W US 9929257W WO 0034442 A3 WO0034442 A3 WO 0034442A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
smooth muscle
muscle cells
application
shear flow
Prior art date
Application number
PCT/US1999/029257
Other languages
French (fr)
Other versions
WO2000034442A2 (en
Inventor
Ann A Lee
Joan Zeltinger
Stephanie M Kladakis
Original Assignee
Advance Tissue Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advance Tissue Sciences Inc filed Critical Advance Tissue Sciences Inc
Priority to IL14356499A priority Critical patent/IL143564A0/en
Priority to CA002353578A priority patent/CA2353578A1/en
Priority to AU21719/00A priority patent/AU2171900A/en
Priority to JP2000586876A priority patent/JP2002531118A/en
Priority to EP99966089A priority patent/EP1144596A2/en
Publication of WO2000034442A2 publication Critical patent/WO2000034442A2/en
Publication of WO2000034442A3 publication Critical patent/WO2000034442A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Vascular Medicine (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to methods for the growth of smooth muscle cells in culture for the production of tissue-engineered grafts or other implantable replacement structures. More specifically, the invention relates to the application of shear flow stress to smooth muscle cells in culture, wherein the cells align perpendicular to the direction of flow, thus more closely approximating the orientation of the cells in vivo. The resulting cultures and methods are useful for the production of improved vascular grafts, vessels and other implantable structures for the correction of defects or abnormal tissues in the body.
PCT/US1999/029257 1998-12-11 1999-12-10 Application of shear flow stress to smooth muscle cells for the production of implantable structures WO2000034442A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
IL14356499A IL143564A0 (en) 1998-12-11 1999-12-10 Application of shear flow stress to smooth muscle cells for the production of implantable structures
CA002353578A CA2353578A1 (en) 1998-12-11 1999-12-10 Application of shear flow stress to smooth muscle cells for the production of implantable structures
AU21719/00A AU2171900A (en) 1998-12-11 1999-12-10 Application of shear flow stress to smooth muscle cells for the production of implantable structures
JP2000586876A JP2002531118A (en) 1998-12-11 1999-12-10 Applying shear flow stress to smooth muscle cells to produce implantable structures
EP99966089A EP1144596A2 (en) 1998-12-11 1999-12-10 Application of shear flow stress to smooth muscle cells for the production of implantable structures

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11176098P 1998-12-11 1998-12-11
US60/111,760 1998-12-11

Publications (2)

Publication Number Publication Date
WO2000034442A2 WO2000034442A2 (en) 2000-06-15
WO2000034442A3 true WO2000034442A3 (en) 2001-11-01

Family

ID=22340317

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/029257 WO2000034442A2 (en) 1998-12-11 1999-12-10 Application of shear flow stress to smooth muscle cells for the production of implantable structures

Country Status (6)

Country Link
EP (1) EP1144596A2 (en)
JP (1) JP2002531118A (en)
AU (1) AU2171900A (en)
CA (1) CA2353578A1 (en)
IL (1) IL143564A0 (en)
WO (1) WO2000034442A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030007954A1 (en) 1999-04-12 2003-01-09 Gail K. Naughton Methods for using a three-dimensional stromal tissue to promote angiogenesis
EP1782849A3 (en) * 1999-04-12 2007-05-23 Theregen, Inc. Three-dimensional stromal tissue
DE10061704A1 (en) * 2000-12-12 2002-06-20 Hans Joerg Bauer Method and device for producing biological tissue in a growth chamber
JP2002315566A (en) * 2001-04-24 2002-10-29 Takagi Ind Co Ltd Cell and tissue-culturing apparatus
JP2005510300A (en) * 2001-11-28 2005-04-21 ウニベルシテ ラバル How to improve the functionality of an organizational structure
US20050019488A1 (en) * 2001-11-30 2005-01-27 Cambridge Polymer Group, Inc., Boston, Ma Layered aligned polymer structures and methods of making same
US7048963B2 (en) 2001-11-30 2006-05-23 Cambridge Polymers Group, Inc. Layered aligned polymer structures and methods of making same
US7387645B2 (en) * 2003-04-25 2008-06-17 Medtronic Vascular, Inc. Cellular therapy to heal vascular tissue
WO2005010172A2 (en) * 2003-07-16 2005-02-03 Boston Scientific Limited Aligned scaffolds for improved myocardial regeneration
EP1689283B1 (en) * 2003-10-22 2015-09-02 Medtrain Technologies, LLC Tissue engineered construct analytical imaging system and method of obtaining and analyzing images of tissue engineered constructs
US9493735B2 (en) 2005-04-15 2016-11-15 Wake Forest University Health Sciences Bioreactor system and method of enhancing functionality of muscle cultured in vitro
JP4919296B2 (en) * 2005-12-26 2012-04-18 国立大学法人大阪大学 Method for culturing three-dimensional tissue including biological shape
JP4989108B2 (en) 2006-05-25 2012-08-01 Ｊｎｃ株式会社 Vascular smooth muscle cell culture method, culture equipment, and medical material obtained by culture
US8058057B2 (en) 2008-05-30 2011-11-15 Corning Incorporated Cell culture apparatus and method
CA2758208A1 (en) 2009-04-09 2010-10-14 Jordan Lancaster Cellular seeding and co-culture of a three dimensional fibroblast construct
US8507263B2 (en) 2009-08-07 2013-08-13 Maria Adelaide Asnaghi Rotating bioreactor
WO2012021814A2 (en) 2010-08-13 2012-02-16 Wake Forest University Health Sciences Methods for making a tissue engineered muscle repair (temr) construct in vitro for implantation in vivo
WO2014174899A1 (en) * 2013-04-23 2014-10-30 国立大学法人徳島大学 Compressed cell or compressed tissue as cell model of pathological condition and manufacturing method therefor
SG11201912185XA (en) 2017-06-16 2020-01-30 Avery Therapeutics Inc Three dimensional tissue compositions and methods of use

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995025547A1 (en) * 1994-03-24 1995-09-28 University Of Washington Devices and methods for implanting transduced cells
WO1997049799A1 (en) * 1996-06-27 1997-12-31 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995025547A1 (en) * 1994-03-24 1995-09-28 University Of Washington Devices and methods for implanting transduced cells
WO1997049799A1 (en) * 1996-06-27 1997-12-31 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CUCINA, A. ET AL.: "Haemodynamic forces modulate simultaneously the release of growth factors and the organisation of cytoskeleton of aortic smooth muscle-cells", MINERVA CARDIOANGIOLOGICA, vol. 44, no. 12, December 1996 (1996-12-01), pages 637 - 643, XP000864786 *
LEE, A.A.: "Mechanisms of fluid shear stress-induced alignement in cultured vascular smooth muscle cells", FASEB JOURNAL, vol. 13, no. 5, 15 March 1999 (1999-03-15), pages A1020, XP000929487 *
RESNICK, N. ET AL.: "Platelet-derived growth factor B chain promoter contains a cis-acting fluid shear-stress-responsive element", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 90, no. 10, 15 May 1993 (1993-05-15), pages 4591 - 4595, XP002075319 *
ZIEGLER, T. ET AL.: "Co-culture of endothelial cells and smooth muscle cells in a flow environment: an improved culture model of the vascular wall?", CELLS AND MATERIALS, vol. 5, no. 2, 1995, pages 115 - 124, XP000864797 *

Also Published As

Publication number Publication date
CA2353578A1 (en) 2000-06-15
IL143564A0 (en) 2002-04-21
WO2000034442A2 (en) 2000-06-15
JP2002531118A (en) 2002-09-24
EP1144596A2 (en) 2001-10-17
AU2171900A (en) 2000-06-26

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