JPS5921813Y2 - Blood granulocyte collection filter - Google Patents

Description

[Detailed Description of the Invention] The present invention relates to a filter for adsorbing and collecting granulocytes from blood.

In recent years, patients with malignant tumors such as acute leukemia have been treated with intensive multidrug therapy, but this often results in temporary devastation of the bone marrow, resulting in a severe decrease in white blood cells and bacteria. The number of deaths due to infectious diseases is increasing.

Even the large-scale use of new antibiotics has little effect on patients with severely reduced granular white blood cells, and infections account for the majority of deaths in leukemia and malignant lymphoma.

It has been clinically confirmed that transfusion of large amounts of granulocytes is highly effective in such cases.

Currently, methods for collecting granulocytes include continuous centrifugation and FL (Filtration Leukophere), which collects granulocytes by attaching them to nylon or polyester fibers.
sis) method, but the former has drawbacks such as poor yield of granulocytes, expensive equipment, complicated operation, and troublesome sterilization of instruments.

In comparison, the FL method has a lower yield of granulocytes than the centrifugation method.
It is much larger, the instruments are cheap, disposable, easy to operate, and safe.

Green wait uses a granulocyte collection filter for the purpose of suppressing side effects during blood transfusions, and has developed a filter filled with nylon fibers (U.S. Patent No. 34623).
The blood was passed through a tube (No. 61) to adsorb and remove granulocytes, and the granulocyte-free blood was used for transfusion.

Djerassi also utilized this filter for the purpose of transfusing granulocytes.

Specifically, a method (U.S. Pat. No. 3,892,236) was performed in which a large amount of granulocytes were collected by placing a filter filled with nylon fibers between the blood collecting pulmonary vein and the blood returning arm vein to continuously circulate blood. .

Large-scale granulocyte transfusion for critically ill patients is said to require 1010 to 1011 granulocytes/transfusion in order to be effective, but the nylon filters mentioned above do not have a very good granulocyte adsorption/recovery rate; It takes a long time to obtain the granulocyte count, which places a heavy burden on the blood donor, and is not good for maintaining the function of the transfused granulocytes.

The present invention was developed in view of the above-mentioned circumstances, and when collecting and collecting a specified number of granulocytes during mass granulocyte transfusion, the circulation time can be significantly shortened compared to conventional methods, reducing the burden on blood donors, and Another object of the present invention is to provide a filter that does not impair the function of collected granulocytes.

A distant relative of the above object is a filter filled with a fibrous material for collecting granulocytes from blood, which has an inlet at one end and an outlet at the other end, and the fibrous material has a wire diameter. 1.0 to 4.0 denier, has a circular or elliptical cross-sectional shape, and has a smooth surface.
The filter is filled in an unheated state to a density of 0.08 to 0.13 g 7 cm3.

Preferably, the fibrous material is either cellulose, nylon-6 or polyester fiber.

When fresh blood granulocytes, which have been anticoagulated with heparin or ion exchange resin, come into contact with foreign substances, they have the ability to phagocytose foreign substances.
A method was used in which granulocytes were adsorbed and removed using cotton wool or the like.

However, there are differences in the adsorption rate depending on the surface charge structure of the object.

The adsorbed granulocytes have a reduced adsorption capacity in the presence of an effluent such as ACD-A solution, and can be recovered by entering the effluent by mechanical stimulation such as hitting the outer surface of the filter. In this case as well, the recovery rate varies depending on the adsorption target.

In addition, when performing the FL method, among blood active components other than granulocytes, platelets have a large influence.

Platelets also have a similar adhesiveness to foreign substances as granulocytes, but
There are also differences in adsorption properties depending on the surface charge, wettability, surface roughness, etc. of the object.

As mentioned above, in the large-volume granulocyte collection method, it is important to set the target object and filter density so that granulocytes can be adsorbed well, can be efficiently collected by washing and draining, and the adsorption loss of platelets is minimal. is an important issue.

As a result of extensive research, the inventor of the present invention found that the most suitable material to be adsorbed is cellulose or nylon-6 fiber, with a wire diameter in the range of 1.0 to 4.0 mm and a circular or elliptical cross-sectional shape. It was concluded that it is necessary to have a smooth surface and to fill the filter with a density of 0.08 to 0.13 g/cm3.

Hereinafter, embodiments of the present invention will be described based on the drawings.

FIG. 1 shows a specific example of a filter for collecting blood granulocytes.

In order to efficiently adsorb and collect granulocytes into a cylinder 1 made of ABS, AS, polycarbonate resin, etc., which has an inlet 2 and an outlet 3, a wire with a diameter of 1.0 to 4.0 mm and a circular or cross-sectional shape is used. Fibrous material 4 with an elliptical and smooth surface
uniformly and with a density of 0.08 to 0.13 g 7c
It is filled to a volume of m3.

Examples of the fibrous material 4 include cellulose, nylon, etc.
6. Thoroughly loosen polyester fibers, etc. before use.

The upper and lower sides of the fibrous material 4 are provided with supporting plates 5a and 5 with holes, respectively.
It is held fixed by b.

FIG. 2 is a schematic diagram of a large-volume granulocyte collection set that constitutes an extracorporeal blood circulation circuit using the granulocyte collection filter of the present invention.

On the blood suction side, there is a suction AVF (Arterial
Venous Fistula) 'e t17 a
For example, a luer connector 7 made of hard vinyl chloride is provided at the tip of the flexible conduit 20 made of vinyl chloride so that it can be connected to the inlet 2 of the above-mentioned granulocyte collection filter via a blood pump. .

Immediately downstream of the suction side luer connector 7, a priming port 8 for injecting physiological saline and a heparin mixture injection port 9 for preventing blood coagulation are provided before the blood is circulated outside the body.

On the blood return side, a cell collection tube 11 made of vinyl chloride is provided which branches downstream of the outlet 3 of the granulocyte collection filter 6 to collect granulocytes, and a blood collection tube 11 containing a blood filtration filter is provided. A drip chamber 12 for creating a drip flow and a bubble detector 13 for electrically detecting bubbles are provided, and are connected to the return AVF set 17b via a Luer connector 16.

Upstream of the return side luer connector 16, a washing liquid recovery tube 15 made of vinyl chloride for recovering physiological saline and a mixed injection port 14 are provided.

Next, the operation of the above configuration will be explained.

The suction and return side AVF sets H7a and 17b are punctured and fixed in the donor's left and right cubital veins, and the donor is injected with heparin (
10,000-15,000 units) are administered.

Prior to blood circulation, the set is filled with physiological saline 21 from the priming port 8, and after degassing the granulocyte filter 6 and other circulation parts, the Luer connector 7.1
6 and AVF sets 1-17a and 17b are connected, and blood circulation is started by the blood pump 10.

At this time, the priming physiological saline 21 is collected from the washing liquid collection tube 15 into a vinyl chloride bag 22 in order to minimize injection into the blood donor.

Extracorporeal blood circulation has a blood flow rate of 40 to 100 ml/sec,
This is done with a circulation time of 2-4 hours.

A necessary amount of heparin (for example, 3000 to 5000 units) per hour is intermittently injected from the heparin mixture injection port 9 on the suction side during circulation, and the blood is anticoagulated.

After the blood circulation is completed, the suction side AVF set l-17a and the luer connector 7 are separated, most of the blood in the set is returned to the donor, and the gap between the drip chamber 12 and the cell collection tube 11 is closed with a Kochel etc., and priming is performed. Port 8 to ACD-Plasma-Saline 23
is poured into the set, the granulocyte collection filter 6 is thoroughly washed, and the adsorbed granulocytes are flushed out from the cell collection tube 11 and the cell collection set 18.
Through Cell Collection PVC Bag 19
to be collected.

At this time, the collection rate of granulocytes can be increased by applying mechanical stimulation such as hitting the outer surface of the granulocyte collection filter 6.

The effluent containing granulocytes collected in the set collection bag 19 is concentrated by centrifugation and infused to a patient in need of granulocytes.

As explained above, the present invention is capable of adsorbing granulocytes in blood.
By providing a filter with a high recovery rate, it is possible to significantly shorten the time required for large-scale granulocyte collection methods, thereby reducing the burden on blood donors and maintaining good granulocyte function. effective, and
All operations can now be performed aseptically using inexpensive instruments, significantly improving safety.

[Brief explanation of the drawing]

FIG. 1 is a diagram showing a specific example of the granulocyte collection filter of the present invention, and FIG. 2 is a schematic diagram of a large-volume granulocyte collection set that configures an extracorporeal blood circulation circuit using the granulocyte collection filter. 1...Cylinder, 2...Inlet, 3...
...Outlet, 4...Fiber material, 5 a, 5 b
... Support plate, 6 ... Granulocyte collection filter, 7, 16 ... Luer connector, 8 ...
... Plumbing Po 1-. 9.14... Mixed injection port, 10... Blood pump, 11...
Cell collection tube, 12...Trip chamber, 13...Bubble detector, 15.
...Washing liquid collection tube, 17a, 17b
...AVF set, 18 ... Cell collection set, 19 ... Cell collection bag, 20 ... Flexible conduit.

Claims (2)

[Scope of utility model registration request] (1) In a filter filled with a fibrous material for collecting granulocytes from blood having an inlet at one end and an outlet at the other end, the fibrous material has a wire diameter of 1.0 to 4.0 mm. , the cross-sectional shape is circular or elliptical, the surface is smooth, and the filter has a 0.080
．． A blood granulocyte collection filter characterized in that it is filled without being heat-treated to a density of 13 g/cm3. (2) The blood granule collection filter according to claim 1, wherein the fibrous material is any of cellulose, nylon-6, or polyester fibers.