ES2128323T3 - REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING IN VITRO AMPLIFICATION OF NUCLEIC ACID USING MODIFIED NUCLEIC ACID BASES. - Google Patents

REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING IN VITRO AMPLIFICATION OF NUCLEIC ACID USING MODIFIED NUCLEIC ACID BASES.

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Publication number
ES2128323T3
ES2128323T3 ES91916353T ES91916353T ES2128323T3 ES 2128323 T3 ES2128323 T3 ES 2128323T3 ES 91916353 T ES91916353 T ES 91916353T ES 91916353 T ES91916353 T ES 91916353T ES 2128323 T3 ES2128323 T3 ES 2128323T3
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ES
Spain
Prior art keywords
nucleic acid
pct
reduction
amplification
specific amplification
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Expired - Lifetime
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ES91916353T
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Spanish (es)
Inventor
John J Sninsky
David H Gelfand
Shirley Y Kwok
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PCT No. PCT/US91/05210 Sec. 371 Date Jan. 5, 1993 Sec. 102(e) Date Jan. 5, 1993 PCT Filed Jul. 23, 1991 PCT Pub. No. WO92/01814 PCT Pub. Date Feb. 6, 1992.Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bags into the amplification reaction products and treating the products by enzymatic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications.
ES91916353T 1990-07-24 1991-07-23 REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING IN VITRO AMPLIFICATION OF NUCLEIC ACID USING MODIFIED NUCLEIC ACID BASES. Expired - Lifetime ES2128323T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55751790A 1990-07-24 1990-07-24
US60915790A 1990-11-02 1990-11-02

Publications (1)

Publication Number Publication Date
ES2128323T3 true ES2128323T3 (en) 1999-05-16

Family

ID=27071456

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91916353T Expired - Lifetime ES2128323T3 (en) 1990-07-24 1991-07-23 REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING IN VITRO AMPLIFICATION OF NUCLEIC ACID USING MODIFIED NUCLEIC ACID BASES.

Country Status (11)

Country Link
US (1) US5418149A (en)
EP (1) EP0540693B1 (en)
JP (1) JP3392863B2 (en)
AT (1) ATE176002T1 (en)
AU (1) AU665338B2 (en)
CA (1) CA2087724C (en)
DE (1) DE69130800T2 (en)
DK (1) DK0540693T3 (en)
ES (1) ES2128323T3 (en)
GR (1) GR3029987T3 (en)
WO (1) WO1992001814A2 (en)

Families Citing this family (179)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
WO1994023069A1 (en) 1993-03-26 1994-10-13 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
DE69428167T2 (en) * 1993-07-01 2002-05-29 F. Hoffmann-La Roche Ag, Basel High temperature reverse transcription reagent and method associated with a polymerase chain reaction
ES2334890T3 (en) * 1993-10-22 2010-03-17 Abbott Laboratories REACTION TUBE AND METHOD OF USE TO MINIMIZE CONTAMINATION.
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6406855B1 (en) * 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
DE4412286A1 (en) * 1994-04-09 1995-10-12 Boehringer Mannheim Gmbh System for contamination-free processing of reaction processes
FR2722799B1 (en) * 1994-07-21 1996-10-04 Parteurop METHOD FOR AMPLIFICATION OF NUCLEIC ACID USING A MODIFIED NUCLEOSIDE, AND DETECTION OF THE AMPLIFICATION PRODUCT USING ANTIBODIES
ZA956776B (en) 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
CA2168712A1 (en) * 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
CA2223050A1 (en) * 1995-06-07 1996-12-19 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
WO1997003210A1 (en) * 1995-07-11 1997-01-30 FORFÁS (trading as BIORESEARCH IRELAND) Glycosylase mediated detection of nucleotide sequences at candidate loci
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US5837442A (en) 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
DE19545320A1 (en) * 1995-12-05 1997-06-12 Boehringer Mannheim Gmbh Thermolabile uracil DNA glycosylase, process for its preparation and use for removing uracil from DNA
US5763184A (en) 1996-01-30 1998-06-09 Roche Molecular Systems, Inc. Nucleotide sequence variation in the ABO glycosyltransferase gene
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) * 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US6436638B1 (en) 1996-05-09 2002-08-20 Metropolitan Water District Of Southern California Cryptosporidium detection method
EP0912761A4 (en) 1996-05-29 2004-06-09 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
DK0866071T3 (en) * 1997-03-20 2005-01-17 Hoffmann La Roche Modified primers
US5958738A (en) 1997-03-24 1999-09-28 Roche Diagnostics Corporation Procedure for subtractive hybridization and difference analysis
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
DE19736062A1 (en) * 1997-08-20 1999-02-25 Boehringer Mannheim Gmbh Reduction of cross-contamination in nucleic acid amplifications
DK2270234T3 (en) 1997-12-08 2013-06-03 California Inst Of Techn Method for Preparation of Polynucleotide and Polypeptide Sequences
ATE469982T1 (en) * 1998-02-04 2010-06-15 Life Technologies Corp DETERMINATION OF THE GENOTYPE OF AN AMPLIFICATION PRODUCT AT MULTIPLE ALLELE SITES
EP1060266A4 (en) * 1998-02-23 2004-09-22 Dana Farber Cancer Inst Inc Method for identifying mismatch repair glycosylase reactive sites, compound and uses thereof
US6174680B1 (en) 1998-12-30 2001-01-16 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof
JP4196236B2 (en) * 1998-03-17 2008-12-17 東洋紡績株式会社 Nucleic acid amplification reagent and sequence-specific nucleic acid amplification method
WO1999050447A1 (en) * 1998-03-27 1999-10-07 Mira Diagnostika Gmbh METHOD AND NUCLEIC ACID COMPOUND FOR BREAKING DOWN NUCLEIC ACID MOLECULES SYNTHESIZED $i(IN VITRO)
CA2266755A1 (en) * 1998-04-24 1999-10-24 James M. Dunn Method for sequencing of nucleic acid polymers
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US7270958B2 (en) * 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
JP2002538415A (en) * 1998-12-30 2002-11-12 デイナ−ファーバー キャンサー インスティチュート，インコーポレイテッド Mutation scanning arrays and methods of using the same
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP1038974A1 (en) * 1999-03-26 2000-09-27 Mira Diagnostica GmbH Method, oligonucleotides suitable for the degradation of in vitro synthezised nucleic acid molecules
EP1041159A3 (en) * 1999-03-26 2000-10-25 MIRA Diagnostika GmbH Method, oligonucleotides suitable for the degradation of in vitro synthesized nucleic acid molecules
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
WO2001036679A2 (en) * 1999-11-15 2001-05-25 Hartwell John G METHODS FOR GENERATING SINGLE STRANDED cDNA FRAGMENTS
US7455965B2 (en) 2000-04-14 2008-11-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
ES2300346T3 (en) * 2000-08-03 2008-06-16 F. Hoffmann-La Roche Ag LINING COMPOUNDS OF NUCLEIC ACIDS CONTAINING ANALOGS OF PIRAZOLO-3,4-D PURINE PYRIMIDINE 2,6-DIAMINE AND ITS USES.
CA2426540A1 (en) * 2000-10-20 2002-07-25 Biocardia, Inc. Leukocyte expression profiling
US20020165673A1 (en) * 2001-01-19 2002-11-07 Allen Morgan Method of self-sampling and analyzing a semen sample
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7222059B2 (en) * 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
PT1499738E (en) 2002-02-21 2008-10-20 Asm Scient Inc Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
US20040005614A1 (en) * 2002-05-17 2004-01-08 Nurith Kurn Methods for fragmentation, labeling and immobilization of nucleic acids
US7273730B2 (en) * 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
EP2390352A1 (en) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20050123956A1 (en) * 2003-09-25 2005-06-09 Affymetrix, Inc. Methods for modifying DNA for microarray analysis
AU2004311882A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
DE602005016221D1 (en) * 2004-02-04 2009-10-08 Qiagen North American Holdings BY NON-FREEZING PROTEIN IMPROVED NUCLEIC ACID AMPLIFICATION
US20050191682A1 (en) * 2004-02-17 2005-09-01 Affymetrix, Inc. Methods for fragmenting DNA
WO2005112544A2 (en) 2004-02-19 2005-12-01 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
US20080311627A1 (en) * 2004-06-17 2008-12-18 Epigenomics Ag Compositions and Methods for Preventing Carry-Over Contamination in Nucleic Acid Amplification Reactions
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
WO2006034009A2 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
WO2006034387A1 (en) 2004-09-21 2006-03-30 Applera Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)
EP1828411B1 (en) * 2004-12-03 2012-11-07 Human Genetic Signatures PTY Ltd Methods for simplifying microbial nucleic acids by chemical modification of cytosines
WO2006122295A2 (en) * 2005-05-11 2006-11-16 Expression Diagnostics, Inc. Methods of monitoring functional status of transplants using gene panels
EP1883707B1 (en) * 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
CA2614444C (en) * 2005-07-07 2015-12-08 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency
JP5027126B2 (en) 2005-07-25 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド Method for multiplexing recombinase polymerase amplification
JP5164845B2 (en) * 2005-09-14 2013-03-21 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド Health status assay
CN103215248B (en) 2005-10-03 2016-05-18 应用生物系统有限责任公司 For composition, method and the kit of amplification of nucleic acid
US20070087059A1 (en) * 2005-10-17 2007-04-19 Frank Everaerts Bioactive delivery matrix compositions and methods
WO2008051290A2 (en) * 2006-04-07 2008-05-02 Xdx, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
BRPI0710889A2 (en) 2006-04-24 2011-08-16 Sigma Alimentos Sa De Cv method for multiple and simultaneous detection and quantification of pathogens by real time polymerase chain reaction
EP2021506B1 (en) 2006-04-28 2016-03-16 Igor Kutyavin Use of products of pcr amplification carrying elements of secondary structure to improve pcr-based nucleic acid detection
WO2007127992A2 (en) 2006-04-28 2007-11-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
EP2038429B1 (en) * 2006-06-30 2013-08-21 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US7993832B2 (en) * 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
WO2008140484A2 (en) * 2006-11-09 2008-11-20 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
FR2914317B1 (en) * 2007-03-30 2012-12-28 Biomerieux Sa METHOD OF DECONTAMINATING A SOLUTION OF UNDESIRABLE NUCLEIC ACIDS
AU2008329549B2 (en) * 2007-11-27 2013-08-22 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
US20100286379A1 (en) * 2007-12-05 2010-11-11 Human Genetic Signatures Pty Ltd. Bisulphite treatment of rna
EP2071034A1 (en) * 2007-12-12 2009-06-17 bioMérieux Method for treating a solution in order to destroy any ribonucleic acid after amplification
EP2222850A4 (en) * 2007-12-20 2011-12-07 Human Genetic Signatures Pty Elimination of contaminants associated with nucleic acid amplification
US8669061B2 (en) * 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies
US8198023B2 (en) 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
WO2010048386A1 (en) * 2008-10-24 2010-04-29 Helicos Biosciences Corporation Methods of sample preparation for nucleic acid analysis for nucleic acids available in limited amounts
US9469867B2 (en) * 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
EP2272976A1 (en) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
US20120052495A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria monocytogenes and use thereof
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
CA3155334A1 (en) 2010-10-22 2012-04-26 T2 Biosystems, Inc. Nmr systems and methods for the rapid detection of analytes
JP5961247B2 (en) 2011-04-07 2016-08-02 アリーア サン ディエゴ， インコーポレイテッド Recombinase polymerase amplification mixture monitoring
JP5830286B2 (en) 2011-07-12 2015-12-09 アークレイ株式会社 Nucleic acid amplification detection method and kit
JP5977000B2 (en) 2011-07-12 2016-08-24 アークレイ株式会社 Nucleic acid amplification detection method and kit
NZ622418A (en) 2011-09-07 2014-12-24 Human Genetic Signatures Pty Molecular detection assay
SG11201401628WA (en) 2011-10-19 2014-05-29 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
GB2513793B (en) 2012-01-26 2016-11-02 Nugen Tech Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN114891871A (en) 2012-08-14 2022-08-12 10X基因组学有限公司 Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
DK2909339T3 (en) 2012-10-18 2018-08-13 Idexx Lab Inc Kits comprehensive controls for nucleic acid amplification
WO2014071315A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Novel compositions, methods and kits for enhancing pcr specificity
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2931919B1 (en) 2012-12-14 2019-02-20 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108753766A (en) 2013-02-08 2018-11-06 10X基因组学有限公司 Polynucleotides bar code generating at
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
GB201312995D0 (en) * 2013-07-19 2013-09-04 Atlas Genetics Ltd Methods and kits for specific nucleic acid amplification and detection
JP6525473B2 (en) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド Compositions and methods for identifying replicate sequencing leads
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
EP3129143B1 (en) 2014-04-10 2022-11-23 10X Genomics, Inc. Method for partitioning microcapsules
JP6423004B2 (en) 2014-05-13 2018-11-14 ラシリック, インコーポレイテッドRASIRC, Inc. Methods and systems for delivering process gases to critical process processing
JP6838969B2 (en) 2014-06-26 2021-03-03 １０エックス ジェノミクス， インコーポレイテッド Method for Analyzing Nucleic Acids Derived from Individual Cells or Cell Populations
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
JP2018505688A (en) 2015-02-24 2018-03-01 １０エックス ゲノミクス，インコーポレイテッド Method for targeted nucleic acid sequence coverage (COVERAGE)
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
CN114250278B (en) 2015-03-13 2024-08-06 生命技术公司 Methods, compositions and kits for capturing, detecting and quantifying small RNAs
EP3283638B1 (en) 2015-04-17 2022-06-08 The Regents of The University of California Methods for detecting agglutination and use of kits in practicing the same
JP6966681B2 (en) 2015-04-24 2021-11-17 アティラ バイオシステムズ インコーポレイテッドAtila Biosystems Incorporated Amplification with primers with limited nucleotide composition
KR101605671B1 (en) 2015-05-12 2016-03-23 주식회사 엔지노믹스 Method for detecting false negative amplification of nucleic acids using polynucleotide comprising deoxyuridine as indicator for UDG activity
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3279337A1 (en) 2016-08-04 2018-02-07 Servizo Galego de Saúde (SERGAS) Use of short probes between 8 and 9 nucleotides in multiplex assays
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP4230746A3 (en) 2017-05-26 2023-11-01 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10155939B1 (en) * 2017-06-15 2018-12-18 New England Biolabs, Inc. Method for performing multiple enzyme reactions in a single tube
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN111051523B (en) 2017-11-15 2024-03-19 10X基因组学有限公司 Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
MX2018008742A (en) 2018-07-16 2020-01-17 Sigma Alimentos Sa De Cv Method and diagnostic kit for simultaneously and multiple detecting a combination of positive gram bacteria and/or negative gram bacteria.
WO2021089643A1 (en) 2019-11-04 2021-05-14 Mirnax Biosens, S.L. Bivalent reverse primer
EP3901286A1 (en) 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
CN113275053A (en) 2020-02-03 2021-08-20 帝肯基因组学公司 Reagent storage system
US20240207805A1 (en) * 2021-04-30 2024-06-27 Nutcracker Therapeutics, Inc. METHODS FOR PERFORMING IN VITRO TRANSCRIPTION USING dUTP
WO2024146937A1 (en) 2023-01-06 2024-07-11 Dna Script Methods for obtaining correctly assembled nucleic acids

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4873192A (en) * 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
EP0451141B1 (en) * 1988-05-20 1998-11-18 F. Hoffmann-La Roche Ag Immobilized sequence-specific probes
ZA899593B (en) * 1988-12-16 1990-09-26 Siska Diagnostics Inc Self-sustained,sequence replication system
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
DK0415755T3 (en) * 1989-09-01 1996-01-22 Life Technologies Inc Method for controlling contamination of oligonucleotide-dependent nucleic acid amplification reactions

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