EP0648239A1 - Biocompatible polymer conjugates - Google Patents
Biocompatible polymer conjugatesInfo
- Publication number
- EP0648239A1 EP0648239A1 EP93916926A EP93916926A EP0648239A1 EP 0648239 A1 EP0648239 A1 EP 0648239A1 EP 93916926 A EP93916926 A EP 93916926A EP 93916926 A EP93916926 A EP 93916926A EP 0648239 A1 EP0648239 A1 EP 0648239A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- collagen
- peg
- conjugate
- polymer
- growth factor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 229920000249 biocompatible polymer Polymers 0.000 title description 5
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 207
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 154
- 239000000203 mixture Substances 0.000 claims abstract description 154
- 229920005615 natural polymer Polymers 0.000 claims abstract description 81
- 102000004127 Cytokines Human genes 0.000 claims abstract description 48
- 108090000695 Cytokines Proteins 0.000 claims abstract description 48
- 239000003102 growth factor Substances 0.000 claims abstract description 46
- 229920001477 hydrophilic polymer Polymers 0.000 claims abstract description 36
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 26
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 26
- 230000003416 augmentation Effects 0.000 claims abstract description 21
- 239000002245 particle Substances 0.000 claims abstract description 20
- 239000007787 solid Substances 0.000 claims abstract description 17
- 230000002163 immunogen Effects 0.000 claims abstract description 14
- 239000012530 fluid Substances 0.000 claims abstract description 11
- 239000003937 drug carrier Substances 0.000 claims abstract description 6
- 241000124008 Mammalia Species 0.000 claims abstract 2
- 102000008186 Collagen Human genes 0.000 claims description 200
- 108010035532 Collagen Proteins 0.000 claims description 200
- 229920001436 collagen Polymers 0.000 claims description 200
- 229920000642 polymer Polymers 0.000 claims description 66
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 60
- 239000007943 implant Substances 0.000 claims description 59
- 238000000034 method Methods 0.000 claims description 32
- 210000000988 bone and bone Anatomy 0.000 claims description 31
- -1 polytetrafluoroethylene Polymers 0.000 claims description 28
- 108060002894 fibrillar collagen Proteins 0.000 claims description 26
- 102000013373 fibrillar collagen Human genes 0.000 claims description 26
- 229920001059 synthetic polymer Polymers 0.000 claims description 25
- 210000001519 tissue Anatomy 0.000 claims description 24
- 150000002148 esters Chemical class 0.000 claims description 23
- 239000011324 bead Substances 0.000 claims description 22
- 238000004132 cross linking Methods 0.000 claims description 22
- 229920002674 hyaluronan Polymers 0.000 claims description 19
- 229960003160 hyaluronic acid Drugs 0.000 claims description 19
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 17
- 230000008439 repair process Effects 0.000 claims description 16
- 239000011541 reaction mixture Substances 0.000 claims description 15
- 235000019731 tricalcium phosphate Nutrition 0.000 claims description 14
- 239000001506 calcium phosphate Substances 0.000 claims description 12
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 12
- AVJBPWGFOQAPRH-FWMKGIEWSA-L dermatan sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@H](OS([O-])(=O)=O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O1 AVJBPWGFOQAPRH-FWMKGIEWSA-L 0.000 claims description 10
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 10
- 239000011236 particulate material Substances 0.000 claims description 10
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 10
- 229940078499 tricalcium phosphate Drugs 0.000 claims description 10
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims description 10
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000011065 in-situ storage Methods 0.000 claims description 7
- 229920001287 Chondroitin sulfate Polymers 0.000 claims description 6
- 230000007547 defect Effects 0.000 claims description 6
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 claims description 6
- 229910010271 silicon carbide Inorganic materials 0.000 claims description 6
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 claims description 5
- 229920000045 Dermatan sulfate Polymers 0.000 claims description 5
- 230000003190 augmentative effect Effects 0.000 claims description 5
- KXKPYJOVDUMHGS-OSRGNVMNSA-N chondroitin sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](OS(O)(=O)=O)[C@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](C(O)=O)O1 KXKPYJOVDUMHGS-OSRGNVMNSA-N 0.000 claims description 5
- 229940059329 chondroitin sulfate Drugs 0.000 claims description 5
- 229940051593 dermatan sulfate Drugs 0.000 claims description 5
- 230000009969 flowable effect Effects 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 150000004676 glycans Chemical class 0.000 claims description 5
- 230000012010 growth Effects 0.000 claims description 5
- 229920001282 polysaccharide Polymers 0.000 claims description 5
- 239000005017 polysaccharide Substances 0.000 claims description 5
- 229920002307 Dextran Polymers 0.000 claims description 4
- 229920002683 Glycosaminoglycan Polymers 0.000 claims description 4
- 102000014150 Interferons Human genes 0.000 claims description 4
- 108010050904 Interferons Proteins 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims description 4
- 239000000017 hydrogel Substances 0.000 claims description 4
- 229940047124 interferons Drugs 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 102000003390 tumor necrosis factor Human genes 0.000 claims description 4
- 102000003951 Erythropoietin Human genes 0.000 claims description 3
- 108090000394 Erythropoietin Proteins 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 3
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims description 3
- 108010025020 Nerve Growth Factor Proteins 0.000 claims description 3
- 102000015336 Nerve Growth Factor Human genes 0.000 claims description 3
- 229920003086 cellulose ether Polymers 0.000 claims description 3
- 108010035886 connective tissue-activating peptide Proteins 0.000 claims description 3
- 229940105423 erythropoietin Drugs 0.000 claims description 3
- 239000008273 gelatin Substances 0.000 claims description 3
- 229920000159 gelatin Polymers 0.000 claims description 3
- 235000019322 gelatine Nutrition 0.000 claims description 3
- 235000011852 gelatine desserts Nutrition 0.000 claims description 3
- 230000000921 morphogenic effect Effects 0.000 claims description 3
- 229940053128 nerve growth factor Drugs 0.000 claims description 3
- 230000002188 osteogenic effect Effects 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 claims description 3
- 229920002379 silicone rubber Polymers 0.000 claims description 3
- 239000004945 silicone rubber Substances 0.000 claims description 3
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical group C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims description 3
- 108010081589 Becaplermin Proteins 0.000 claims description 2
- 229920000858 Cyclodextrin Polymers 0.000 claims description 2
- 102400001368 Epidermal growth factor Human genes 0.000 claims description 2
- 101800003838 Epidermal growth factor Proteins 0.000 claims description 2
- 102000003971 Fibroblast Growth Factor 1 Human genes 0.000 claims description 2
- 108090000386 Fibroblast Growth Factor 1 Proteins 0.000 claims description 2
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims description 2
- 102000015696 Interleukins Human genes 0.000 claims description 2
- 108010063738 Interleukins Proteins 0.000 claims description 2
- 102000013275 Somatomedins Human genes 0.000 claims description 2
- 229940116977 epidermal growth factor Drugs 0.000 claims description 2
- 229940047122 interleukins Drugs 0.000 claims description 2
- 108010000685 platelet-derived growth factor AB Proteins 0.000 claims description 2
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 2
- NMWKYTGJWUAZPZ-WWHBDHEGSA-N (4S)-4-[[(4R,7S,10S,16S,19S,25S,28S,31R)-31-[[(2S)-2-[[(1R,6R,9S,12S,18S,21S,24S,27S,30S,33S,36S,39S,42R,47R,53S,56S,59S,62S,65S,68S,71S,76S,79S,85S)-47-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-methylbutanoyl]amino]-3-methylbutanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-phenylpropanoyl]amino]-4-oxobutanoyl]amino]-3-carboxypropanoyl]amino]-18-(4-aminobutyl)-27,68-bis(3-amino-3-oxopropyl)-36,71,76-tribenzyl-39-(3-carbamimidamidopropyl)-24-(2-carboxyethyl)-21,56-bis(carboxymethyl)-65,85-bis[(1R)-1-hydroxyethyl]-59-(hydroxymethyl)-62,79-bis(1H-imidazol-4-ylmethyl)-9-methyl-33-(2-methylpropyl)-8,11,17,20,23,26,29,32,35,38,41,48,54,57,60,63,66,69,72,74,77,80,83,86-tetracosaoxo-30-propan-2-yl-3,4,44,45-tetrathia-7,10,16,19,22,25,28,31,34,37,40,49,55,58,61,64,67,70,73,75,78,81,84,87-tetracosazatetracyclo[40.31.14.012,16.049,53]heptaoctacontane-6-carbonyl]amino]-3-methylbutanoyl]amino]-7-(3-carbamimidamidopropyl)-25-(hydroxymethyl)-19-[(4-hydroxyphenyl)methyl]-28-(1H-imidazol-4-ylmethyl)-10-methyl-6,9,12,15,18,21,24,27,30-nonaoxo-16-propan-2-yl-1,2-dithia-5,8,11,14,17,20,23,26,29-nonazacyclodotriacontane-4-carbonyl]amino]-5-[[(2S)-1-[[(2S)-1-[[(2S)-3-carboxy-1-[[(2S)-1-[[(2S)-1-[[(1S)-1-carboxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(1H-imidazol-4-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid Chemical compound CC(C)C[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H]1CSSC[C@H](NC(=O)[C@@H](NC(=O)[C@@H]2CSSC[C@@H]3NC(=O)[C@H](Cc4ccccc4)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](Cc4c[nH]cn4)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H]4CCCN4C(=O)[C@H](CSSC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](Cc4c[nH]cn4)NC(=O)[C@H](Cc4ccccc4)NC3=O)[C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc3ccccc3)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N3CCC[C@H]3C(=O)N[C@@H](C)C(=O)N2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](Cc2c[nH]cn2)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)C(C)C)C(=O)N[C@@H](Cc2c[nH]cn2)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](Cc2ccc(O)cc2)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1)C(=O)N[C@@H](C)C(O)=O NMWKYTGJWUAZPZ-WWHBDHEGSA-N 0.000 claims 1
- OFMQLVRLOGHAJI-FGHAYEPSSA-N (4r,7s,10s,13r,16s,19r)-n-[(2s,3r)-1-amino-3-hydroxy-1-oxobutan-2-yl]-19-[[(2r)-2-amino-3-phenylpropanoyl]amino]-10-[3-(diaminomethylideneamino)propyl]-7-[(1r)-1-hydroxyethyl]-16-[(4-hydroxyphenyl)methyl]-13-(1h-indol-3-ylmethyl)-3,3-dimethyl-6,9,12,15,18 Chemical compound C([C@H]1C(=O)N[C@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(=O)N[C@@H](C(SSC[C@@H](C(=O)N1)NC(=O)[C@H](N)CC=1C=CC=CC=1)(C)C)C(=O)N[C@@H]([C@H](O)C)C(N)=O)[C@@H](C)O)C1=CC=C(O)C=C1 OFMQLVRLOGHAJI-FGHAYEPSSA-N 0.000 claims 1
- 101800003265 Beta-thromboglobulin Proteins 0.000 claims 1
- 102400001362 Beta-thromboglobulin Human genes 0.000 claims 1
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims 1
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 1
- 102000006747 Transforming Growth Factor alpha Human genes 0.000 claims 1
- 102000011117 Transforming Growth Factor beta2 Human genes 0.000 claims 1
- 101800004564 Transforming growth factor alpha Proteins 0.000 claims 1
- 101800000304 Transforming growth factor beta-2 Proteins 0.000 claims 1
- 230000002281 colonystimulating effect Effects 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 229940072041 transforming growth factor beta 2 Drugs 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 28
- 238000000576 coating method Methods 0.000 abstract description 17
- 239000011248 coating agent Substances 0.000 abstract description 14
- 210000004872 soft tissue Anatomy 0.000 abstract description 14
- 238000002347 injection Methods 0.000 abstract description 10
- 239000007924 injection Substances 0.000 abstract description 10
- 239000000126 substance Substances 0.000 abstract description 6
- 239000007972 injectable composition Substances 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 80
- 239000000463 material Substances 0.000 description 54
- 210000000845 cartilage Anatomy 0.000 description 37
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 28
- 239000002953 phosphate buffered saline Substances 0.000 description 28
- 238000006243 chemical reaction Methods 0.000 description 27
- 235000018102 proteins Nutrition 0.000 description 24
- 239000000501 collagen implant Substances 0.000 description 23
- 238000009472 formulation Methods 0.000 description 19
- 241001465754 Metazoa Species 0.000 description 18
- 229920001427 mPEG Polymers 0.000 description 18
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 16
- 238000002360 preparation method Methods 0.000 description 16
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 12
- 239000008188 pellet Substances 0.000 description 12
- 230000007704 transition Effects 0.000 description 12
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 11
- 230000035882 stress Effects 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 239000004698 Polyethylene Substances 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 10
- 230000021615 conjugation Effects 0.000 description 10
- 239000000499 gel Substances 0.000 description 10
- 230000007062 hydrolysis Effects 0.000 description 10
- 238000006460 hydrolysis reaction Methods 0.000 description 10
- 230000004962 physiological condition Effects 0.000 description 10
- 229920000573 polyethylene Polymers 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 108090000765 processed proteins & peptides Proteins 0.000 description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 230000008901 benefit Effects 0.000 description 8
- 230000002500 effect on skin Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000001125 extrusion Methods 0.000 description 8
- 238000002513 implantation Methods 0.000 description 8
- 238000011534 incubation Methods 0.000 description 8
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 8
- 229910052697 platinum Inorganic materials 0.000 description 8
- 230000000717 retained effect Effects 0.000 description 8
- 210000000481 breast Anatomy 0.000 description 7
- 238000000113 differential scanning calorimetry Methods 0.000 description 7
- 230000005847 immunogenicity Effects 0.000 description 7
- 239000012528 membrane Substances 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 125000003277 amino group Chemical group 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 238000012377 drug delivery Methods 0.000 description 6
- 102000004196 processed proteins & peptides Human genes 0.000 description 6
- 239000007858 starting material Substances 0.000 description 6
- 230000029087 digestion Effects 0.000 description 5
- 239000002657 fibrous material Substances 0.000 description 5
- JFCQEDHGNNZCLN-UHFFFAOYSA-N glutaric acid Chemical compound OC(=O)CCCC(O)=O JFCQEDHGNNZCLN-UHFFFAOYSA-N 0.000 description 5
- VANNPISTIUFMLH-UHFFFAOYSA-N glutaric anhydride Chemical compound O=C1CCCC(=O)O1 VANNPISTIUFMLH-UHFFFAOYSA-N 0.000 description 5
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 5
- 125000005647 linker group Chemical group 0.000 description 5
- 235000018977 lysine Nutrition 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 206010002329 Aneurysm Diseases 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 4
- 102000004142 Trypsin Human genes 0.000 description 4
- 108090000631 Trypsin Proteins 0.000 description 4
- 206010053613 Type IV hypersensitivity reaction Diseases 0.000 description 4
- 208000024780 Urticaria Diseases 0.000 description 4
- 108010006886 Vitrogen Proteins 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- 210000004204 blood vessel Anatomy 0.000 description 4
- 239000002775 capsule Substances 0.000 description 4
- 239000000512 collagen gel Substances 0.000 description 4
- 210000004207 dermis Anatomy 0.000 description 4
- 238000007598 dipping method Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 230000001976 improved effect Effects 0.000 description 4
- 238000002844 melting Methods 0.000 description 4
- 230000008018 melting Effects 0.000 description 4
- 230000003472 neutralizing effect Effects 0.000 description 4
- 239000003208 petroleum Substances 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 229920001296 polysiloxane Polymers 0.000 description 4
- 230000035484 reaction time Effects 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 210000005070 sphincter Anatomy 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 230000005951 type IV hypersensitivity Effects 0.000 description 4
- 208000027930 type IV hypersensitivity disease Diseases 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- NHJVRSWLHSJWIN-UHFFFAOYSA-N 2,4,6-trinitrobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O NHJVRSWLHSJWIN-UHFFFAOYSA-N 0.000 description 3
- NWAGXLBTAPTCPR-UHFFFAOYSA-N 5-(2,5-dioxopyrrolidin-1-yl)oxy-5-oxopentanoic acid Chemical compound OC(=O)CCCC(=O)ON1C(=O)CCC1=O NWAGXLBTAPTCPR-UHFFFAOYSA-N 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 102000007644 Colony-Stimulating Factors Human genes 0.000 description 3
- 108010071942 Colony-Stimulating Factors Proteins 0.000 description 3
- 239000004971 Cross linker Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 3
- 229920001734 PEG propionaldehyde Polymers 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 108010067787 Proteoglycans Proteins 0.000 description 3
- 102000016611 Proteoglycans Human genes 0.000 description 3
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 3
- 239000003929 acidic solution Substances 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 229940047120 colony stimulating factors Drugs 0.000 description 3
- 210000002808 connective tissue Anatomy 0.000 description 3
- 210000001608 connective tissue cell Anatomy 0.000 description 3
- GYZLOYUZLJXAJU-UHFFFAOYSA-N diglycidyl ether Chemical compound C1OC1COCC1CO1 GYZLOYUZLJXAJU-UHFFFAOYSA-N 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 210000003979 eosinophil Anatomy 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 239000003517 fume Substances 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 210000002216 heart Anatomy 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 229920000669 heparin Polymers 0.000 description 3
- 229960002897 heparin Drugs 0.000 description 3
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 3
- 230000008105 immune reaction Effects 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 210000005036 nerve Anatomy 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 206010033675 panniculitis Diseases 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 238000000518 rheometry Methods 0.000 description 3
- 239000002002 slurry Substances 0.000 description 3
- 238000013268 sustained release Methods 0.000 description 3
- 239000012730 sustained-release form Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 230000008467 tissue growth Effects 0.000 description 3
- 150000003626 triacylglycerols Chemical class 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 230000029663 wound healing Effects 0.000 description 3
- ZJIFDEVVTPEXDL-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) hydrogen carbonate Chemical compound OC(=O)ON1C(=O)CCC1=O ZJIFDEVVTPEXDL-UHFFFAOYSA-N 0.000 description 2
- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical compound ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 description 2
- OCKGFTQIICXDQW-ZEQRLZLVSA-N 5-[(1r)-1-hydroxy-2-[4-[(2r)-2-hydroxy-2-(4-methyl-1-oxo-3h-2-benzofuran-5-yl)ethyl]piperazin-1-yl]ethyl]-4-methyl-3h-2-benzofuran-1-one Chemical compound C1=C2C(=O)OCC2=C(C)C([C@@H](O)CN2CCN(CC2)C[C@H](O)C2=CC=C3C(=O)OCC3=C2C)=C1 OCKGFTQIICXDQW-ZEQRLZLVSA-N 0.000 description 2
- 241001631457 Cannula Species 0.000 description 2
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 2
- 241000700198 Cavia Species 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 2
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 206010015150 Erythema Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 2
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 2
- 208000005422 Foreign-Body reaction Diseases 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 241000906446 Theraps Species 0.000 description 2
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 2
- 108010009583 Transforming Growth Factors Proteins 0.000 description 2
- 102000009618 Transforming Growth Factors Human genes 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 125000002947 alkylene group Chemical group 0.000 description 2
- 238000004873 anchoring Methods 0.000 description 2
- 150000008064 anhydrides Chemical class 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 235000011010 calcium phosphates Nutrition 0.000 description 2
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000001427 coherent effect Effects 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 238000013270 controlled release Methods 0.000 description 2
- MGNCLNQXLYJVJD-UHFFFAOYSA-N cyanuric chloride Chemical compound ClC1=NC(Cl)=NC(Cl)=N1 MGNCLNQXLYJVJD-UHFFFAOYSA-N 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- VILAVOFMIJHSJA-UHFFFAOYSA-N dicarbon monoxide Chemical compound [C]=C=O VILAVOFMIJHSJA-UHFFFAOYSA-N 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 231100000321 erythema Toxicity 0.000 description 2
- 125000001033 ether group Chemical group 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 229940126864 fibroblast growth factor Drugs 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N hydrazine Substances NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 210000003041 ligament Anatomy 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 238000010534 nucleophilic substitution reaction Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 150000003904 phospholipids Chemical class 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 239000002861 polymer material Substances 0.000 description 2
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 231100000241 scar Toxicity 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000012421 spiking Methods 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- KZNICNPSHKQLFF-UHFFFAOYSA-N succinimide Chemical compound O=C1CCC(=O)N1 KZNICNPSHKQLFF-UHFFFAOYSA-N 0.000 description 2
- 210000002435 tendon Anatomy 0.000 description 2
- 239000010936 titanium Substances 0.000 description 2
- 229910052719 titanium Inorganic materials 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 238000003260 vortexing Methods 0.000 description 2
- 239000008215 water for injection Substances 0.000 description 2
- JPSKCQCQZUGWNM-UHFFFAOYSA-N 2,7-Oxepanedione Chemical compound O=C1CCCCC(=O)O1 JPSKCQCQZUGWNM-UHFFFAOYSA-N 0.000 description 1
- FALRKNHUBBKYCC-UHFFFAOYSA-N 2-(chloromethyl)pyridine-3-carbonitrile Chemical compound ClCC1=NC=CC=C1C#N FALRKNHUBBKYCC-UHFFFAOYSA-N 0.000 description 1
- 125000004042 4-aminobutyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])N([H])[H] 0.000 description 1
- 208000010392 Bone Fractures Diseases 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 206010051814 Eschar Diseases 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical class NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 229920002971 Heparan sulfate Polymers 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 206010062575 Muscle contracture Diseases 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108010067035 Pancrelipase Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 208000006735 Periostitis Diseases 0.000 description 1
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 1
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229920002385 Sodium hyaluronate Polymers 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- 241000133063 Trixis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000008065 acid anhydrides Chemical class 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 1
- 230000001195 anabolic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- PFYXSUNOLOJMDX-UHFFFAOYSA-N bis(2,5-dioxopyrrolidin-1-yl) carbonate Chemical compound O=C1CCC(=O)N1OC(=O)ON1C(=O)CCC1=O PFYXSUNOLOJMDX-UHFFFAOYSA-N 0.000 description 1
- OMAHFYGHUQSIEF-UHFFFAOYSA-N bis(2,5-dioxopyrrolidin-1-yl) oxalate Chemical compound O=C1CCC(=O)N1OC(=O)C(=O)ON1C(=O)CCC1=O OMAHFYGHUQSIEF-UHFFFAOYSA-N 0.000 description 1
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 1
- 230000008468 bone growth Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910001576 calcium mineral Inorganic materials 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000004068 calcium phosphate ceramic Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- PFKFTWBEEFSNDU-UHFFFAOYSA-N carbonyldiimidazole Chemical compound C1=CN=CN1C(=O)N1C=CN=C1 PFKFTWBEEFSNDU-UHFFFAOYSA-N 0.000 description 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 229940107200 chondroitin sulfates Drugs 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 208000006111 contracture Diseases 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000011243 crosslinked material Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 231100000333 eschar Toxicity 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009778 extrusion testing Methods 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- ZFKJVJIDPQDDFY-UHFFFAOYSA-N fluorescamine Chemical compound C12=CC=CC=C2C(=O)OC1(C1=O)OC=C1C1=CC=CC=C1 ZFKJVJIDPQDDFY-UHFFFAOYSA-N 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000011597 hartley guinea pig Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 210000003701 histiocyte Anatomy 0.000 description 1
- 239000012456 homogeneous solution Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 125000004356 hydroxy functional group Chemical group O* 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 230000008102 immune modulation Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 229940102223 injectable solution Drugs 0.000 description 1
- 229940102213 injectable suspension Drugs 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 210000000867 larynx Anatomy 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 210000000111 lower esophageal sphincter Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000009607 mammography Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- 108700005457 microfibrillar Proteins 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- YTVNOVQHSGMMOV-UHFFFAOYSA-N naphthalenetetracarboxylic dianhydride Chemical compound C1=CC(C(=O)OC2=O)=C3C2=CC=C2C(=O)OC(=O)C1=C32 YTVNOVQHSGMMOV-UHFFFAOYSA-N 0.000 description 1
- 239000006225 natural substrate Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000000269 nucleophilic effect Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000011164 ossification Effects 0.000 description 1
- 230000002138 osteoinductive effect Effects 0.000 description 1
- 210000003101 oviduct Anatomy 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000003239 periodontal effect Effects 0.000 description 1
- 210000003460 periosteum Anatomy 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 108010017843 platelet-derived growth factor A Proteins 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 239000004626 polylactic acid Substances 0.000 description 1
- 229920005597 polymer membrane Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000009145 protein modification Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 210000003625 skull Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229940010747 sodium hyaluronate Drugs 0.000 description 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
- NCGJACBPALRHNG-UHFFFAOYSA-M sodium;2,4,6-trinitrobenzenesulfonate Chemical compound [Na+].[O-][N+](=O)C1=CC([N+]([O-])=O)=C(S([O-])(=O)=O)C([N+]([O-])=O)=C1 NCGJACBPALRHNG-UHFFFAOYSA-M 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008227 sterile water for injection Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000004003 subcutaneous fat Anatomy 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 229940014800 succinic anhydride Drugs 0.000 description 1
- 229960002317 succinimide Drugs 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 230000009772 tissue formation Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 210000005062 tracheal ring Anatomy 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 210000005166 vasculature Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/12—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L31/125—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/102—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0033—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/04—Macromolecular materials
- A61L29/043—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/04—Macromolecular materials
- A61L29/044—Proteins; Polypeptides; Degradation products thereof
- A61L29/045—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/04—Macromolecular materials
- A61L29/06—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/14—Materials characterised by their function or physical properties, e.g. lubricating compositions
- A61L29/16—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/041—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/043—Proteins; Polypeptides; Degradation products thereof
- A61L31/044—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/08—Materials for coatings
- A61L31/10—Macromolecular materials
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/495—Transforming growth factor [TGF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H1/00—Macromolecular products derived from proteins
- C08H1/06—Macromolecular products derived from proteins derived from horn, hoofs, hair, skin or leather
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J189/00—Adhesives based on proteins; Adhesives based on derivatives thereof
- C09J189/04—Products derived from waste materials, e.g. horn, hoof or hair
- C09J189/06—Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
- A61F2/06—Blood vessels
- A61F2/07—Stent-grafts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2310/00—Prostheses classified in A61F2/28 or A61F2/30 - A61F2/44 being constructed from or coated with a particular material
- A61F2310/00005—The prosthesis being constructed from a particular material
- A61F2310/00365—Proteins; Polypeptides; Degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/236—Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/426—Immunomodulating agents, i.e. cytokines, interleukins, interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2666/00—Composition of polymers characterized by a further compound in the blend, being organic macromolecular compounds, natural resins, waxes or and bituminous materials, non-macromolecular organic substances, inorganic substances or characterized by their function in the composition
- C08L2666/02—Organic macromolecular compounds, natural resins, waxes or and bituminous materials
Definitions
- This invention relates to biocompatible conjugates formed by covalently binding two or more polymers together and, specifically, to conjugates formed by binding a naturally occurring polymer or derivative thereof to a synthetic hydrophilic polymer, such as polyethylene glycol (PEG) , and to compositions, components, and implants comprised of such conjugates.
- a synthetic hydrophilic polymer such as polyethylene glycol (PEG)
- a number of naturally occurring but biologically inert polymers are known. Examples of such include collagen and various glycosaminoglycans such as hyaluronic acid, chondroitin sulfates, chitin, and heparin. Derivatives of these polymers have also been produced and certain derivatives have been formulated for medical use.
- a number of synthetic biologically inert polymers are also known, e.g., polyethylene glycol (PEG).
- PEG polyethylene glycol
- Conjugates are formed by covalently binding insoluble, naturally occurring, biologically inert polymers and derivatives thereof to synthetic, hydrophilic polymers such as polyethylene glycol (PEG) .
- the naturally-occurring polymers and derivatives thereof include polysaccharides such as hyaluronic acid, proteoglycans such as chondroitin sulfate A (4-sulfate) , chondroitin sulfate C (6-sulfate) , and dermatan sulfate (chondroitin sulfate B) ; chitin; heparin and heparin sulfate; dextrans such as cyclodextran, hydroxylethyl cellulose, cellulose ether, and starch; lipids (esters of fatty acids with trihydroxyl alcohol glycerol) such as triglycerides, phospholipids, and the like.
- the synthetic hydrophilic polymer is preferably polyethylene glycol and derivatives thereof having a weight average molecular weight in the range of from about 100 to about 100,000 preferably about 1,500 to 20,000.
- Compositions and components may be formulated using the conjugates and other components such as pharmaceutically acceptable fluid carriers to form injectable formulations, and/or biologically active proteins such as cytokines and growth factors.
- the biocompatible conjugates of the invention generally contain large amounts of water when formed and can be dehydrated to form a relatively solid object which will expand in size five-fold or more upon rehydration. Implants can be coated with conjugate formulation, and articles such as tubes and strings can be constructed using specific conjugates and formulations in order to obtain the particular desired characteristics.
- the biocompatible conjugates of the invention are applied and used in a variety of medical and pharmaceutical applications.
- the most basic embodiment includes the biocompatible conjugates and pharmaceutical compositions formulated using these conjugates, which compositions include pharmaceutically acceptable fluid carriers of varying types and amounts.
- various natural polymers are covalently bound to synthetic polymers such as polyethylene glycol.
- the specific polymers are chosen based on the end use and characteristics desired.
- different types of covalent bonds can be used, including ester, ether and urethane linkages.
- One of the most important uses for certain conjugates and compositions of the invention is in methods of augmenting soft tissue.
- compositions are formulated in a flowable form and injected into patients, such as into facial areas, to provide for soft tissue augmentation.
- the method can be varied so that the reaction between the naturally occurring polymer and the synthetic polymer occurs in situ.
- the conjugates can be dehydrated and then ground into particles, suspended in an inert nonaqueous carrier, and injected into a patient. After injection, the carrier will be removed by natural physiological conditions and the particles will rehydrate and swell to their original size. Strings formed from conjugates can also be injected to obtain soft tissue augmentation.
- conjugates and conjugate compositions of the invention can be combined with cytokines or growth factors to promote tissue growth, and/or further combined with particles, fibers or other materials to increase the structural integrity of the compositions so that they can also be used in the augmentation of hard tissue, such as bone and cartilage.
- Other uses for conjugates include coatings for various medical devices to be incorporated within the body, including catheters, bone implants, and platinum wires to treat aneurysms.
- the conjugates may also be formulated into various ophthalmic devices, such as lenticules or corneal shields. Conjugate formulations may also be extruded, molded, and/or formed into shapes such as strings and tubes which have medical uses such as in sutures and blood vessel or nerve repair.
- a primary object of the invention is to provide biocompatible conjugates formed by covalently binding polymers such as polyethylene glycol to non-immunogenic forms of naturally occurring, insoluble, biologically inert polymers and derivatives thereof.
- Another object of the invention is to provide the conjugates with different types of bonds and to provide compositions containing conjugates in pharmaceutically acceptable fluid carriers suitable for injection.
- Another object of the invention is to provide a composition for tissue augmentation produced by forming the conjugates, dehydrating the conjugates to form a solid, grinding the solid into particles, and suspending the particles in a non-aqueous fluid carrier for injection to the site of augmentation, at which time the particles will rehydrate and expand in size about five ⁇ fold.
- Still another object of the invention is to provide tubes made of the conjugate materials.
- biocompatible conjugates comprising particular types of covalent bonds, such as ether linkages, can be used to provide a high degree of stability over long periods of time under physiological conditions.
- conjugates can be formed using a variety of natural and synthetic polymers, each occuring in a range of molecular weights in order to adjust the physical and chemical characteristics of the resulting composition.
- biocompatible conjugates have superior handling characteristics as compared with conventional collagen compositions.
- biocompatible conjugate compositions generate a decreased immune reaction as compared with conventional compositions.
- Another feature of the present invention is that the- biocompatible conjugate compositions have improved moldability, malleability, and elasticity as compared with conventional compositions.
- compositions and conjugates in combination with pharmaceutically active proteins such as cytokines and growth factors in order to improve the activity and available half-life of such cytokines or growth factors under physiological conditions.
- Another advantage of the present invention is that an ether linkage may be used to connect the natural and synthetic polymers which bond is resistant to breakage due to hydrolysis.
- Figure 1 is a bar graph showing the results of swellability testing for different strings
- Figures 2, 3, 4 and 5 are each bar graphs showing the results of testing physical characteristics of different strings
- Figures 6 and 7 each show comparisons of the DSC measurements of collagen containing compositions
- Figure 8 shows the reaction scheme for reacting a carboxyl group of hyaluronic acid with PEG-hydrazine
- Figure 9 shows a reaction scheme wherein an acetyl group of hyaluronic acid is reacted with succinimydyl- PEG;
- Figure 10 shows the structures of chondroitin sulfate A, chondroitin sulfate C and dermatan sulfate (chondroitin sulfate B) ;
- Figure 11 is a reaction scheme showing a nucleophilic substitution reaction of an ester with PEG
- Figure 12 shows the reaction scheme of reacting polyethylene with a multifunctional activated form of E- PEG
- Figure 13 shows a reaction scheme reacting polyethylene with a multifunctional activated form of S- PEG
- Figure 14 is a table showing the results of measurements of strings produced in accordance with Example 13;
- Figure 15 is a table showing physical data relating to strings comprised of collagen and collagen conjugates
- Figure 16 is a table showing data obtained from measurements of materials produced in accordance with Example 15;
- Figure 17 is a table showing the results of measurements obtained on materials produced in accordance with Example 18. Detailed Description of Preferred Embodiments of the Invention
- collagen refers to all forms of collagen, including telopeptide-containing collagen, and atelopeptide collagen which has been processed or modified.
- the collagen may be of human or animal origin and may be produced by recombinant techniques. Forms included are the various types, i.e., Type I, II, III, fibrillar, non-fibrillar, etc.
- Collagen is a material which is the major protein component of bone, cartilage, skin, and connective tissue in animals.
- Collagen in its native form is typically a rigid, rod- shaped molecule approximately 300 nm long and 1.5 nm in diameter. It is composed of three collagen polypeptides which form a tight triple helix.
- the collagen polypeptides are characterized by a long midsection having the repeating sequence -Gly-X-Y-, where X and Y are often proline or hydroxyproline, bounded at each end by the "telopeptide" regions, which constitute less than about 5% of the molecule.
- the telopeptide regions of the collagen chains are typically responsible for the naturally occuring crosslinking between chains, as well as for the immunogenicity of the protein.
- Collagen occurs in several "types" having differing physical properties. The most abundant types are Types I-III.
- the present disclosure includes these and other known types of collagen including natural collagen and collagen which is processed or modified, i.e., various collagen derivatives.
- Collagen is typically isolated from natural sources, such as bovine hide, cartilage, or bones.
- Bones are usually dried, defatted, crushed, and demineralized to extract collagen, while hide and cartilage are usually minced and digested with proteolytic enzymes (other than collagenase) .
- proteolytic enzymes other than collagenase
- dehydrated refers to compositions that have been air dried or lyophilized to remove substantially all unbound water.
- natural polymers and “natural polymer” as used herein refer to biologically inert, insoluble, naturally occurring, biocompatible polymers and derivatives thereof.
- natural polymers include polysaccharides such as hyaluronic acid; proteoglycans such as chondroitin sulfate A (4-sulfate) , chondroitin sulfate C (6-sulfate) , and dermatan sulfate (chondroitin sulfate B) ; dextrans such as cyclodextrin, hydroxylethyl cellulose, cellulose ether, and starch; lipids (esters of fatty acids with trihydroxyl alcohol glycerol) such as triglycerides, phospholipids, and mixtures and derivatives thereof.
- the term is intended to specifically exclude biologically active natural polymers such as DNA, RNA, and proteins.
- biologically inert conjugates such as DNA, RNA, and proteins.
- biocompatible conjugates and “biologically inert biocompatible conjugates” are used interchangeably herein.
- the terms refer to biologically inert, insoluble, biocompatible conjugates of the present invention wherein a natural polymer is covalently bound to the synthetic hydrophilic polymer.
- the conjugates have some characteristics similar to the natural polymers in that they are biologically inert, insoluble, nontoxic and do not generate a significant immune reaction when incorporated into a living being.
- synthetic hydrophilic polymer refers to a synthetic polymer having an average molecular weight and composition which renders the polymer essentially hydrophilic, but not completely water-soluble. Preferred polymers are highly purified such that the polymer is pharmaceutically pure. Most hydrophilic polymers can be rendered water-soluble by incorporating a sufficient number of oxygen (or less frequently, nitrogen) atoms available for forming hydrogen bonds in aqueous solutions. Preferred polymers are hydrophilic, but not necessarily soluble. Hydrophilic polymers used herein include polyethylene glycol (PEG), polyoxyethylene, poly ethylene glycol, polytrimethylene glycols, polyvinylpyrrolidones, and derivatives thereof, with PEG being particularly preferred.
- PEG polyethylene glycol
- PEG polyoxyethylene
- poly ethylene glycol polytrimethylene glycols
- polyvinylpyrrolidones polyvinylpyrrolidones
- the polymers can be linear or multiply branched and will not be substantially crosslinked.
- Other suitable polymers include polyoxyethylene- polyoxypropylene block polymers and copolymers. Polyoxyethylene-polyoxypropylene block polymers having an ethylene diamine nucleus (and thus having four ends) are also available and may be used in the practice of the invention. Naturally occurring polymers such as proteins, starch, cellulose, heparin, and the like are expressly excluded from the scope of this definition. All suitable polymers will be non-toxic, non ⁇ inflammatory, and non-i munogenic when administered subcutaneously, and will preferably be essentially nondegradable in vivo over a period of at least several months.
- the hydrophilic polymer may increase the hydrophilicity of the natural polymer, but does not render it water-soluble.
- Presently preferred hydrophilic polymers are mono-, di-, and multifunctionally activated polyethylene glycols (PEG) .
- Monofunctional PEG has only one reactive hydroxy group, while difunctional PEG has reactive groups at each end of the molecule.
- Monofunctional PEG preferably has a weight average molecular weight between about 100 and about 15,000, more preferably between about 200 and about 8,000, and most preferably about 4,000.
- Difunctional PEG preferably has a weight average molecular weight of about 400 to about 100,000, more preferably about 3,000 to about 20,000.
- Multifunctional PEG preferably has an average molecular weight between about 3,000 and 100,000.
- the term "multifunctional” is used herein to refer to synthetic polymers having two or more reactive groups per molecule and, as such, encompasses the term "difunctional.
- PEG can be rendered monofunctional by forming an alkylene ether group at one end.
- the alkylene ether group may be any suitable alkoxy radical having 1-6 carbon atoms, for example, methoxy, ethoxy, propoxy, 2-propoxy, butoxy, hexyloxy, and the like. Methoxy is presently preferred.
- Difunctionally activated PEG is provided by allowing a reactive hydroxy group at each end of the linear molecule.
- the reactive groups are preferably at the ends of the polymer, but may be provided along the length thereof.
- Multifunctionally activated molecules are capable of crosslinking the compositions of the invention, and may be used to attach cytokines •or growth factors to natural polymers. In connection with the synthetic polymers, certain abbreviations are used as follows: monomethoxypolyethylene glycol (mPEG) ; difunctional PEG Succinimydyl Glutarate (SG-PEG) ; difunctional PEG
- Succinimydyl S-PEG
- difunctional PEG Succinimydyl carbonate SC-PEG
- difunctional PEG propion aldehyde A-PEG
- difunctional PEG glycidyl ether E-PEG
- dPEG is used to encompass difunctionally activated polyethylene glycols of a variety of types.
- chemically conjugated means attached through a covalent chemical bond.
- a synthetic hydrophilic polymer and natural polymer or derivative thereof are preferably directly bound to each other via a covalent bond(s) but may be chemically conjugated by using a linking radical, such that the synthetic polymer and natural polymer are each bound to the radical, but not directly to each other.
- biocompatible conjugate refers to a natural polymer chemically conjugated to a synthetic hydrophilic polymer, within the meaning of this invention.
- natural polymer/PEG (or “PEG/natural polymer”) denotes a composition of the invention wherein a natural polymer is chemically conjugated to PEG.
- Natural polymer/PEG refers to a natural polymer of the invention chemically conjugated to difunctionally activated PEG, wherein the polymer molecules may be crosslinked.
- the synthetic polymer may be "chemically conjugated" to the natural polymer by means of a number of different types of chemical linkages.
- the conjugation can be via an ester or a urethane linkage, but is more preferably by means of an ether linkage.
- An ether linkage is preferred in that it can be formed without the use of toxic chemicals and is not readily susceptible to hydrolysis in vjLvfi.
- molecular weight refers to the weight average molecular weight of a number of molecules in any given sample, as commonly used in the art.
- a sample of PEG 2,000 might contain a statistical mixture of polymer molecules ranging in weight from, for example, 1,500 to 2,500 daltons, with one molecule differing slightly from the next over a range.
- Specification of a range of molecular weight indicates that the average molecular weight may be any value between the limits specified, and may include molecules slightly outside those limits.
- a molecular weight range of about 800 to about 20,000 indicates an average molecular weight of at least about 800, ranging up to about 20 kDa.
- available lysine residue refers to lysine side chains exposed on the outer surface of natural polymer molecules, which are positioned in a manner to allow reaction with activated PEG.
- the number - of available lysine residues may be determined by reaction with sodium 2,4,6-trinitrobenzenesulfonate (TNBS) .
- treat and “treatment” as used herein refer to augmentation, repair, prevention, or alleviation of defects, particularly defects due to loss or absence of soft tissue or soft tissue support, or to loss or absence of bone or cartilage.
- the term “treat” includes the use of strings of the invention to suture wounds, and the use of tubes of the invention to repair, replace, or augment a channel in the body of a living being, in particular a human being.
- “treat” and “treatment” also refer to the prevention, maintenance, or alleviation of disorders or diseases using a biologically active protein coupled to and/or mixed with a conjugate-containing composition of the invention.
- treatment of soft tissue includes augmentation of soft tissue, for example, implantation of conjugates of the invention to restore normal or desirable dermal contours, as in the removal of dermal creases or furrows, or as in the replacement of subcutaneous fat in maxillary areas where the fat is lost due to aging or in the augmentation of submucosal tissue, such as the urinary or lower esophageal sphincters.
- Treatment of bone and cartilage includes the use of conjugates, and particularly natural polymer/PEG in combination with suitable particulate materials, to replace or repair bone tissue, for example, in the treatment of bone nonunions or fractures.
- Treatment of bone also includes use of conjugate-containing compositions with or without additional bone growth factors.
- Compositions comprising conjugates with ceramic particles, preferably calcium phosphate ceramics such as hydroxyapatite and/or tricalcium phosphate, are particularly useful for the repair of stress-bearing bone- due to its high tensile strength.
- cytokine and growth factor are used to describe biologically active molecules and active peptides (which may be either naturally occurring or synthetic) which aid in healing or regrowth of normal tissue.
- the function of cytokines and growth factors is two-fold: 1) they can incite local cells to produce new collagen or tissue, or 2) they can attract cells to the site in need of correction. As such, cytokines and growth factors serve to encourage "biological anchoring" of the implant within the host tissue.
- the cytokines or growth factors can either be admixed with the conjugate or chemically coupled to the conjugate.
- cytokines such as interferons (IFN) , tumor necrosis factors (TNF) , interleukins, colony stimulating factors (CSFs) , or growth factors such as osteogenic factor extract (OFE) , epidermal growth factor (EGF) , transforming growth factor (TGF) alpha, TGF-0 (including any combination of TGF-/?s) , TGF-01, TGF-02, platelet derived growth factor (PDGF-AA, PDGF-AB, PDGF-BB) , acidic fibroblast growth factor (FGF) , basic FGF, connective tissue activating peptides (CTAP) , /3-thromboglobulin, insulin-like growth factors, erythropoietin (EPO) , nerve growth factor (NGF) , bone morphogenic protein (BMP) , osteogenic factors, and the like.
- IFN interferons
- TNF tumor necrosis factors
- CSFs colony stimulating factors
- OFF osteo
- cytokines or growth factors can facilitate the regrowth and remodeling of an implant into normal tissue, or may be used in the treatment of wounds.
- one may chemically link the cytokines or growth factors to the biocompatible conjugate by employing a suitable amount of multifunctional polymer molecules during synthesis.
- the cytokine or growth factors may then be attached to the free polymer ends by the same method used to attach PEG to any natural polymer or derivative thereof or by any other suitable method.
- Conjugates incorporated with cytokines or growth factors may serve as effective controlled release drug delivery systems.
- the chemical linkage between the cytokine and the conjugate it is possible to vary the effect with respect to the release of the cytokine or growth factor. For example, when an "ester” linkage is used, the linkage is more easily broken under physiological conditions, allowing for sustained release of the growth factor or cytokine from the matrix. However, when an "ether” linkage is used, the bonds are not easily broken and the cytokine or growth factor will remain in place for longer periods of time with its active sites exposed, providing a biological effect on the natural substrate for the active site of the protein. It is possible to include a mixture of conjugates with different linkages so as to obtain variations in the effect with respect to the release of the cytokine or growth factor, i.e., the sustained release effect can be modified to obtain the desired rate of release.
- tissue growth-promoting amount refers to the amount of cytokine or growth factor needed in order to stimulate tissue growth to a detectable degree.
- Tissue in this context, includes connective tissue, bone, cartilage, epidermis and dermis, blood, and other tissues. The actual amount which is determined to be an effective amount will vary depending on factors such as the size, condition, sex and age of the patient and can be more readily determined by the caregiver.
- sufficient amount is that amount which, when mixed with the conjugate, renders it in the physical form desired, for example, injectable solution, injectable suspension, plastic or malleable implant, rigid stress-bearing implant, strings, tubes and so forth.
- injectable formulations generally include an amount of fluid carrier sufficient to render the composition smoothly injectable, whereas malleable implants have substantially less carrier and have a clay ⁇ like consistency.
- Rigid stress-bearing implants may include no carrier at all and have a high degree of structural integrity.
- the amount of the carrier can be varied and adjusted depending on the particular conjugate used and the end result desired. Such adjustments will be apparent to those skilled in the art upon reading this disclosure.
- suitable particulate material refers to a particulate material which is substantially insoluble in water, non-immunogenic, biocompatible, and immiscible with the biocompatible conjugates of the invention.
- the particles of material may be fibrillar, or may range in size from about 20 to 250 ⁇ m in diameter and be bead-like or irregular in shape.
- Exemplary particulate materials include, without limitation, fibrillar crosslinked collagen, gelatin beads, crosslinked collagen-dPEG particles, polytetrafluoroethylene beads, silicone rubber beads, hydrogel beads, silicon carbide beads, and glass beads.
- Preferred particulate materials are calcium phosphates, most preferably hydroxyapatite and/or tricalcium phosphate.
- solid implant refers to any solid object which is designed for insertion and use within the body, and includes bone and cartilage implants (e.g., artificial joints, retaining pins, cranial plates, and the like, of metal, plastic and/or other materials) , strings which can be used as sutures or injected for soft tissue augmentation, breast implants (e.g., silicone gel envelopes, foam forms, and the like) , catheters and cannulas intended for long-term use (beyond about three days) , artificial organs and vessels formed from tubes of the invention (e.g., artificial hearts, pancreases, kidneys, blood vessels, and the like) , drug delivery devices (including monolithic implants, pumps and controlled release devices such as Alzet ® minipumps, steroid pellets for anabolic growth or contraception, and the like) , sutures for dermal or internal use, periodontal membranes, lenticules, corneal shields, platinum wires for aneurysm treatment, and the like.
- suitable fibrous material refers to a fibrous material which is substantially insoluble in water, non-immunogenic, biocompatible, and immiscible with the biocompatible conjugates of the invention.
- the fibrous material may comprise a variety of materials having these characteristics and is combined with compositions of the conjugate in order to form and/or provide structural integrity to various implants or devices such as tubes comprised of conjugates used in connection with medical and pharmaceutical uses.
- the conjugate compositions of the invention can be coated on the "suitable fibrous material", which can then be wrapped around a bone to provide structural integrity to the bone.
- the "suitable fibrous material” is useful in forming the "solid implants" of the invention.
- the injectable reaction mixture compositions are injected or otherwise applied to a site in need of augmentation and allowed to crosslink at the site of injection.
- Suitable sites will generally be intradermal or subcutaneous regions for augmenting dermal support, at the site of bone fractures for wound healing and bone repair, and within sphincter tissue for sphincter augmentation (e.g., for restoration of continence) .
- aqueous mixture includes liquid solutions, suspensions, dispersions, colloids, and the like containing a natural polymer and water.
- NFC cartilage refers to a composition of the invention which resembles cartilage in physical consistency.
- NFC cartilage is prepared from nonfibrillar collagen (e.g., collagen in solution) and is crosslinked with a hydrophilic polymer, especially using dPEG.
- dPEG nonfibrillar collagen
- NFC cartilage may contain about 0-20% fibrillar collagen.
- NFC cartilage is generally prepared by adding dPEG in acidic solution to an acidic solution of collagen, and allowing conjugation to occur prior to neutralization.
- NFC-FC cartilage refers to a composition similar to NFC cartilage, wherein the percentage of fibrillar collagen is about 20-80%.
- NFC-FC cartilage is generally prepared by adding dPEG in a neutralizing buffer to an acidic solution of collagen.
- the neutralizing buffer causes collagen fibril formation during the conjugation process.
- FC cartilage refers to a composition of the invention which is prepared from fibrillar collagen and a difunctional hydrophilic polymer. FC cartilage may generally be prepared using dPEG and fibrillar collagen in neutral solutions/suspensions.
- a natural polymer or derivative thereof must be chemically conjugated to a synthetic hydrophilic polymer.
- the synthetic hydrophilic polymer is activated and then reacted directly with the natural polymer.
- hydroxyl or amino groups present on the natural polymer can be activated and the activated groups will react with the polymer to form the conjugate.
- a linking group with activated hydroxyl or amino groups thereon can be combined with the synthetic polymer and natural polymer in a manner so as to concurrently react the natural and synthetic polymers to form the biocompatible conjugate.
- conjugates of the invention are to be used in the human body, it is important that all of the components, including both polymers and linking group, if used, form a conjugate that is unlikely to be reacted to or rejected by the patient. Accordingly, toxic and/or immunoreactive components are not preferred as starting materials. Some preferred starting materials and methods of forming conjugates are described further below.
- the polymer must be biocompatible, relatively insoluble, yet hydrophilic, and is preferably one or more forms of polyethylene glycol (PEG) due to its known biocompatibility.
- PEG polyethylene glycol
- Various forms of PEG are extensively used in the modification of biologically active molecules because PEG can be formulated to have a wide range of solubilities and because it lacks toxicity, antigenicity, immunogenicity, and does not typically interfere with the enzymatic activities and/or conformations of peptides. Further, PEG is generally non-biodegradable and is easily excreted from most living organisms including humans.
- the first step in forming the conjugates of the invention generally involves the functionalization of the PEG molecule.
- Various functionalized polyethylene glycols have been used effectively in fields such as protein modification (see Abuchowski et al., Enzymes as Drugs. John Wiley & Sons: New York, NY (1981) pp. 367-
- polyethylene glycol which has been found to be particularly useful is monomethoxy-polyethylene glycol (mPEG) , which can be activated by the addition of a compound such as cyanuric chloride, then coupled to a protein (see Abuchowski et al., J. Biol. Chem. (1977) 252:3578. which is incorporated herein by reference) .
- mPEG monomethoxy-polyethylene glycol
- Activated forms of PEG can be made from reactants which can be purchased commercially.
- One form of activated PEG which has been found to be particularly useful in connection with the present invention is mPEG-succinate-N-hydroxysuccinimide ester (SS-PEG) (see Abuchowski et al., Cancer Biochem. Biphys. (1984) 2:175, which is incorporated herein by reference) .
- SS-PEG mPEG-succinate-N-hydroxysuccinimide ester
- ester linkages can be used in connection with the present invention, they are not particularly preferred in that they undergo hydrolysis when subjected to physiological conditions over extended periods of time (see Dreborg et al., Crit. Rev. Therap. Drug Carrier Syst. (1990) .6:315; and Ulbrich et al., J. Makromol. Chem. (1986) 187:1131. both of which are incorporated herein by reference) .
- Another means of attaching the PEG to a protein can be by means of a carbamate linkage (see Beauchamp et al., Anal. Biochem. (1983) 131:25; and
- activated PEG compounds can be used in connection with the formation of a wide range of inert, biocompatible conjugates held together by a variety of different types of bonds. Such conjugates provide a range of improved, unexpected characteristics and as such can be used to form the various compositions and articles of the present invention.
- the conjugates of the present invention can be prepared by covalently binding a variety of different types of synthetic hydrophilic polymers to a natural polymer or derivatives thereof.
- the final product or conjugate obtained must have a number of characteristics, such as being biocompatible and non-immunogenic, it has been found that it is useful to use polyethylene glycol as the synthetic hydrophilic polymer.
- the polyethylene glycol must be modified in order to provide activated groups on one or preferably both ends of the molecule so that covalent binding can occur between the PEG and the natural polymer.
- Some specific functionalized forms of PEG are shown structurally below, as are the reaction products obtained by reacting these functionalized forms of PEG with a natural polymer or derivative thereof.
- the first functionalized PEG is difunctionalized PEG succinimidyl glutarate, referred to herein as (SG-PEG) .
- the structural formula of this molecule and the reaction product obtained by reacting it with a natural polymer (represented by NTL-PLYM) with an amine group NH 2 thereon is shown below in Formula 1:
- PEG succinimidyl Another difunctionally activated form of PEG is referred to as PEG succinimidyl (S-PEG) .
- S-PEG PEG succinimidyl
- the structure of Formula 2 includes an "ether" linkage between the difunctionalized PEG and the natural polymers on each end which ether linkage is not subject to hydrolysis. This is distinct from the conjugate of Formula 1, wherein an ester linkage is provided. The ester linkage is subject to hydrolysis under physiological conditions.
- PEG succinimidyl carbonate SC-PEG
- the structural formula of this compound and the conjugate formed by reacting SC-PEG with collagen is shown in Formula 5. Although this conjugate includes a urethane linkage, the conjugate has been found not to have a high degree of stability under physiological conditions.
- the PEG can be derivatized to form difunctional PEG propion aldehyde (A-PEG) , which is shown in Formula 6, as is the conjugate formed by the reaction of A-PEG with a natural polymer or derivative thereof.
- A-PEG difunctional PEG propion aldehyde
- A-PEG Difunctional PEG Propion Aldehyde
- E-PEG difunctional PEG glycidyl ether
- the conjugates formed using the functionalized forms of PEG vary depending on the functionalized form of PEG used in the reaction. Further, the final product can be varied with respect to its characteristics by changing the molecular weight of the PEG. In general, the stability of the conjugate is improved by eliminating any ester linkages between the PEG and the natural polymer and including ether and/or urethane linkages. In certain situations, it is desirable to include the weaker ester linkages so that the linkages are gradually broken by hydrolysis under physiological conditions, breaking apart the matrix and releasing a component held therein, such as a growth factor or cytokine. By varying the chemical structure of the linkages, the rate of sustained release can be varied. 2. PEG Conjugation of Polysaccharides:
- Hyaluronic acid comprises a polymer of the following repeating monomeric units, as shown in Formula 8.
- Hyaluronic acid can be conjugated to PEG using a number of different methods. Preferred methods include modification of the carboxyl group and modification of the acetyl group.
- Preferred methods include modification of the carboxyl group and modification of the acetyl group.
- a reaction scheme showing a modification of a carboxyl group of hyaluronic acid using PEG-hydrazine is shown in figure 8.
- a reaction scheme showing a modification of an acetyl group with succinimydyl-PEG (S-PEG) is shown in figure 9.
- chondroitin sulfate A, chondroitin sulfate C, and dermatan sulfate are of similar structure to hyaluronic acid and can be conjugated to PEG by creating a derivative using much the same methods used to derivatize hyaluronic acid.
- PEG Conjugation of Polyethylene A number of different activated PEGs, both difunctional and multifunctional, can be used to crosslink polyethylene. Difunctional or multifunctional E-PEG can be used for crosslinking polyethylene due to the ease of opening the highly strained three-member ring. The bond angles of the ring, which average 60 degrees, are considerably smaller than the normal tetrahedral carbon angles of 109 degrees. Because polyethylene is not readily denatured at elevated temperatures (as are many natural polymers, such as collagen) , E-PEG may be used to crosslink polyethylene at elevated temperatures.
- the crosslinking reaction between the natural polymer and synthetic polymer may be performed in vitro. or a reaction mixture may be injected for crosslinking in situ.
- crosslinked biocompatible conjugates resemble cartilage, and are useful as substitutes therefor (e.g., cranial onlay, ear and nose reconstruction, and the like) .
- multifunctional polymers may also be used to covalently bind natural polymers to other proteins, particularly cytokines or growth factors, for compositions particularly suited for wound healing, osteogenesis, and immune modulation.
- Such tethering of growth factors or cytokines to biocompatible polymers such as collagen provides an effective slow- release drug delivery system.
- Ether linkages might be used to bond the natural and synthetic polymers whereas ester linkages might be used to attach a cytokine or growth factor and thereby obtain slow release of the cytokine or growth factor.
- Suitable collagens for use in the present invention include all types of collagen, including natural telopeptide-containing collagen which may be used in situations where immune response sensitivity is not significant. However, for most applications, non- immunogenic atelopeptide collagen, in particular types I, II and III, is preferred.
- Collagens may be soluble (for example, commercially available Vitrogen ® 100 collagen- in-solution) and may or may not have the telopeptide regions.
- the collagen will be reconstituted fibrillar atelopeptide collagen, for example, Zyderm ® Collagen Implant (ZCI) or atelopeptide collagen in solution (CIS) .
- ZCI Zyderm ® Collagen Implant
- CIS atelopeptide collagen in solution
- Various forms of collagen are available commercially, or may be prepared by the processes described in, for example, U.S. Pat. Nos. 3,949,073; 4,488,911; 4,424,208; 4,582,640; 4,642,117;
- Non-fibrillar, atelopeptide, reconstituted collagen is preferred in order to form certain products.
- Methods for conjugating collagen to synthetic hydrophilic polymers such as PEG are described in detail in U.S. Patent No. 5,162,430.
- compositions of the invention comprise natural polymers or derivatives thereof chemically conjugated to a selected synthetic hydrophilic polymer or polymers.
- Natural polymers such as collagen derivatives contain a number of available amino and hydroxy groups which may be used to bind the synthetic hydrophilic polymer.
- the polymer may be bound using a "linking group", as the native hydroxy or amino groups on the natural polymer and synthetic polymer frequently require activation before they can be linked.
- dicarboxylic anhydrides e.g., glutaric or succinic anhydride
- a polymer derivative e.g., succinate
- a convenient leaving group for example, N-hydroxysuccinimide, N,N' -disuccinimidyl oxalate, N,N' -disuccinimidyl carbonate, and the like. See also Davis, U.S. Pat. No
- Presently preferred dicarboxylic anhydrides that are used to form polymer-glutarate compositions include glutaric anhydride, adipic anhydride, l,8-naphthalene dicarboxylic anhydride, and 1,4,5,8-naphthalenetetracarboxylic dianhydride.
- the polymer thus activated is then allowed to react with the natural polymer to form a biocompatible conjugate of the invention.
- a pharmaceutically pure form of monomethylpolyethylene glycol (mPEG) (mw 5,000) is reacted with glutaric anhydride (pure form) to create mPEG glutarate.
- the glutarate derivative is then reacted with N-hydroxysuccinimide to form a succinimidyl monomethylpolyethylene glycol glutarate.
- the succinimidyl ester (mPEG*, denoting the activated PEG intermediate) is then capable of reacting with free amino groups (lysine residues) present on certain natural polymers.
- the reaction results in a natural polymer-PEG conjugate of the invention wherein one end of the PEG molecule is free or nonbound.
- the coupling reaction may be carried out using any known method for derivatizing proteins and synthetic polymers.
- the number of available lysines conjugated may vary from a single residue to 100% of the lysines, preferably 10%-50%, and more preferably 20-30%.
- the number of reactive lysine residues may be determined by standard methods, for example by reaction with TNBS.
- the bonds connecting the PEG to any natural polymer and/or other molecules may be ester, ether, and/or urethane bonds and are preferably ether bonds.
- Formulations suitable for repair of bone defects or nonunions may be prepared by providing high concentration compositions of biocompatible conjugates, optionally in admixture with suitable particulate materials.
- the linkage between the collagen and polymer is preferably an ether linkage in order to avoid deterioration due to the hydrolysis of the ester linkages.
- Such conjugate/particulate compositions may be malleable or rigid, depending on the.amount of liquid incorporated.
- Formulations for treatment of stress-bearing bone are preferably dried and rigid, and will generally comprise between about 45% and 85% particulate calcium phosphate mineral, for example hydroxyapatite or tricalcium phosphate, or mixtures thereof.
- the tensile strength and rigidity may be further increased by heating the composition under vacuum at about 60-90°C, preferably about 75°C, for about 5 to 15 hours, preferably about 10 hours.
- Malleable compositions may be used for repair of non-stressed bone or cartilage.
- the activated mPEG* may be replaced, in whole or in part, by difunctionally activated PEG (dPEG*, e.g., non- methylated PEG which is then activated at each end) , thus providing a crosslinked or partially crosslinked composition.
- dPEG* difunctionally activated PEG
- Such compositions are, however, quite distinct from conventionally crosslinked collagen compositions (e.g., using heat, radiation, glutaraldehyde, and the like) , as the long-chain synthetic hydrophilic polymer imparts a substantial hydrophilic character to the composition.
- approximately 1-20% of the PEG is difunctionally activated PEG.
- the character of the composition may be adjusted as desired, by varying the amount of difunctionally activated PEG included.
- difunctionally activated PEG* (substantially 100% at pH 7) is used to crosslink a natural polymer or derivative thereof.
- CIS about 3-100 mg/mL, preferably about 10-40 mg/mL
- dPEG* difunctional PEG activated at each end by addition of an acid anhydride having a leaving group such as succinimide
- an acid anhydride having a leaving group such as succinimide
- a cartilaginoid collagen-polymer conjugate may also be prepared by mixing a dPEG* solution (pH 3) with collagen-in-solution between two syringes to homogeneity, then casting into a suitable container (e.g., a Petri dish).
- a suitable container e.g., a Petri dish.
- NFC-FC conjugate cartilage contains approximately 1-40% fibrillar collagen.
- the characteristics of the final product may be adjusted by varying the initial reactants and reaction conditions.
- natural polymers other than collagen can be used.
- increased concentrations of the natural polymer or the PEG provide a denser, less porous product.
- the pH of the collagen solution and the dPEG* solution compositions may be producing over a wide range of fibrillar collagen content.
- the denser formulations may be cast or molded into any shape desired, for example into sheets, membranes, tubes, cylinders, strings, cords, ropes, and the like. Certain shapes may be produced by extrusion.
- Biocompatible polymer conjugates can also be used as coatings for breast implants.
- the surface of a standard silicone-shell implant can be chemically derivatized to provide active binding sites for di- or multifunctional PEG bound to a natural polymer resulting in a three-part conjugate as follows: (natural polymer-PEG-silicone) .
- the presence of the conjugate coating bound directly to the silicone via PEG will serve to reduce scar tissue formation and capsular contracture. Unlike typical coated breast implants, scar tissue will not be able to grow between the conjugate coating and the surface of the implant itself.
- the conjugate can be formed into a hollow sphere for use as a breast implant shell.
- the shell can then be filled with a radiolucent material, such as triglycerides, to facilitate mammography.
- the injectable conjugate formulations may be used to coat implants, catheters, tubes (e.g., for blood vessel replacement), meshes (e.g., for tissue reinforcement) , strings, and the like.
- Biocompatible conjugate formulations can also be used to coat platinum wires, which can then be administered to the site of an aneurysm via catheter.
- Gels may be prepared with various polymer concentrations and different reaction times.
- CIS is the preferred starting material when the desired properties are high density, rigidity, viscosity, and. translucence.
- fibrillar collagen preferably atelopeptide fibrillar collagen such as ZCI
- ZCI atelopeptide fibrillar collagen
- CIS-based materials are presently preferred for coating articles designed for implantation, such as catheters and stress-bearing bone implants.
- the CIS material is linked to the PEG by an ether bond.
- compositions of the invention are also useful for coating articles for implantation or relatively long-term residence within the body.
- Such surface treatment renders the object non-immunogenic and, as such, reduces the incidence of foreign body reactions.
- compositions of the invention to catheters, cannulas, bone prostheses, cartilage replacements, breast implants, minipumps, and other drug delivery devices, artificial organs, and the like.
- Application may be accomplished by dipping the object into the reaction mixture while crosslinking is occurring and allowing the adherent viscous coating to dry.
- One may pour, brush, or otherwise apply the reaction mixture if dipping is not feasible.
- the object may be dipped in a viscous collagen-in-solution bath, or in a fibrillar collagen solution until the object is completely coated.
- the collagen solution is fixed to the object by dipping the collagen-coated object into a dPEG* (pH 7) solution bath, then allowing the collagen-polymer coated object to dry.
- viscous collagen-in-solution is mixed with a dPEG* (pH 3) solution and polymerized rapidly, as described above.
- the object is dipped in the acidic collagen-polymer solution and cured by dipping the coated object into a neutralizing buffer containing about 20% by weight dPEG* (pH 7) , resulting in a collagen- polymer coated object.
- the biocompatible conjugates of the present invention can be used to produce a variety of different types of coated implants.
- the conjugates can be formed such as by binding a synthetic polymer such as PEG to a natural polymer such as hyaluronic acid.
- the biocompatible conjugate formed can be coated onto the surface of any type of implant device and will be useful in improving the biocompatibility of the implant. It is also possible to use multifunctional PEG. When multifunctional PEG is used, another active site on the PEG can be used to bind a biologically active compound such as a cytokine. When the three-part conjugate is formed, it can be coated onto the surface of the implant.
- the inclusion of a conjugate comprised of cytokine-PEG-hyaluronic acid on the surface of the implant promotes integration of growth of the surrounding cells into the implant.
- a multifunctional synthetic polymer such as multifunctional PEG is used.
- One of the active sites of the multifunctional PEG is connected to a natural polymer such as hyaluronic acid or collagen while another active site of the multifunctional PEG is allowed to react directly with an activated site on the surface of the implant.
- the conjugate is covalently bound directly to the surface of the implant.
- many implants are used to augment or repair bone and require a tight fit within the space where the implant is to be placed. When such is the case, it is desirable to create a coated implant wherein the conjugate used for the coating is initially comprised of a large amount of water, e.g., a collagen-PEG conjugate.
- Coated implants are preferably coated with a conjugate formed with ether linkages in that it is desirable to maintain the conjugate when used in a living body, and the ether linkages are less susceptible to hydrolysis than the ester linkage.
- compositions of the invention containing biologically active cytokines or growth factors such as EGF and TGF-0 are prepared by mixing an appropriate amount of the cytokine or growth factor into the composition, or by combining the cytokine or growth factor with a natural polymer prior to treatment with activated PEG.
- cytokine or growth factor a biologically active cytokine or growth factor
- a degree of crosslinking may be established, along with conjugates consisting of a natural polymer bound to a cytokine or growth factor by a synthetic hydrophilic polymer.
- the cytokine or growth factor is first reacted with a molar excess of dPEG* in a dilute solution over a 3 to 4 hour period.
- the cytokine or growth factor is preferably provided at a concentration of about l ⁇ g/mL to about 5 mg/mL, while the dPEG* is preferably added to a final concentration to provide a 30- to 50- fold molar excess.
- the resulting conjugated cytokine is then added to an aqueous collagen mixture (about 1 to about 60 mg/mL) at pH 7-8 and allowed to react further.
- the resulting composition is allowed to stand overnight at ambient temperature.
- the pellet is collected by centrifugation and is washed with PBS by vigorous vortexing in order to remove any non-bound cytokine or growth factor molecules.
- the biocompatible conjugates are used to form elongated cylinders or strings.
- the strings have a diameter and range of about 0.10mm to about 20mm and more preferably a diameter of about 0.25mm to about 2.5mm.
- the strings may be any length, but preferably have a length in the range of about 0.25cm to about 25cm. The length and diameter of the string will depend, to a large extent, upon the desired use.
- the strings may be produced by molding or extrusion of the conjugate material. Strings which dissolve in tissue could be used as surgical sutures and would be comprised of conjugates using ester linkages which are broken by hydrolysis.
- the strings can be cut into small pieces, dehydrated and injected for soft tissue augmentation.
- the strings can be modified to include other biologically active components such as cytokines or growth factors to encourage further tissue deposition for soft tissue augmentation.
- Membranous Forms Flexible sheets or membranous forms may be prepared by methods known in the art, for example, U.S. Patent Nos. 4,600,533; 4,412,947; and 4,242,291. These methods can be used to produce membranes using the biocompatible conjugates of the present invention.
- a natural polymer such as a high concentration (10-100 mg/mL) CIS or fibrillar collagen (preferably atelopeptide fibrillar collagen such as ZCI) is cast into a flat sheet container.
- a solution of mPEG* (having a molecular weight of approximately 5,000) is added to the cast collagen solution and allowed to react overnight at room temperature.
- the resulting collagen- polymer conjugate is removed from the reaction solution using a sterile spatula or the like and washed with PBS to remove excess unreacted mPEG*.
- the resulting conjugate may then be compressed under constant pressure to form a uniform flat sheet or mat, which is then dried to form a membranous implant of the invention. More flexible membranous forms are achieved by using lower concentrations of the natural polymer (e.g., collagen) and high synthetic polymer concentrations.
- CIS at room temperature, is mixed with a buffer solution and incubated at 37°C overnight. The resulting gel is compressed under constant pressure, dried, and desalted by washing. The resulting membrane is then crosslinked by treating with dPEG*, washed, and then dried at low temperature.
- CIS or fibrillar collagen (10-100 mg/mL) is cast into a flat sheet container. A solution of dPEG* (22-50% w/v) is added to the cast collagen. The mixture is allowed to react over several hours at room temperature. Shorter reaction times result in more flexible membranes.
- the resulting collagen-polymer membrane may be optionally dehydrated under a vacuum oven or by lyophilization or air-drying.
- Biocompatible conjugates may also be prepared in the form of sponges by lyophilizing an aqueous slurry of the composition following conjugation.
- the biocompatible conjugates of the present invention can be used to form tubes by molding or extrusion.
- the tubes have an outer diameter in the range of 0.25mm to about 5.0cm and inner diameter in the range of 0.05mm to about 4.9cm.
- the tubes have a generally circular cross section with respect to their inner and outer diameters.
- the tubes may be of any length, they generally have a length of more than 10mm and, more preferably, greater than 10cm.
- the tubes may be produced with the conjugates having any type of linkages, including ester, ether, or urethane linkages, it is preferable to produce the tubes using ether linkages.
- the tubes can be used to repair various types of channel in a living being such as veins, arteries, and fallopian tubes. However, ' the use of the tubes is not limited as such.
- compositions of the invention have a variety of uses.
- Malleable, plastic compositions may be prepared as injectable formulations which are suitable for dermal augmentation, for example, for filling in dermal creases and providing support for skin surfaces.
- Such compositions are also useful for augmenting sphincter tissue, (e.g., for restoration of continence).
- the formulation may be injected directly into the sphincter tissue to increase bulk and permit the occluding tissues to meet more easily and efficiently.
- These compositions may be homogeneous or may be prepared as suspensions of small microgel conjugate particles or beads which are delivered in a nonaqueous fluid carrier. The beads/particles rehydrate and swell in situ. This has the advantage over commercial preparations in that less volume of product needs to be injected to achieve the desired connection.
- aqueous collagen mixture is combined with a low-concentration dPEG* solution, mixed, and the combination injected or applied before the viscosity increases sufficiently to render injection difficult (usually about 20 minutes) .
- Mixing may be accomplished by passing the mixture between two syringes equipped with Luer lock hubs, or through a single syringe having dual compartments (e.g., double barrel).
- the composition crosslinks in situ, and may additionally crosslink to the endogenous tissue, anchoring the implant in place.
- collagen preferably fibrillar collagen
- the dPEG* concentration is preferably about 0.1 to about 3%, although concentrations as high as 30% may be used if desired.
- the mixture is injected directly into the site in need of augmentation, and causes essentially no inflammation or foreign body reaction.
- One may additionally include particulate materials in the collagen reaction mixture, for example, hydrogel or collagen-dPEG beads, or hydroxyapatite/ tricalcium phosphate particles, to provide a bulkier or more rigid implant after crosslinking.
- compositions of the invention may be prepared in a form that is dense and rigid enough to substitute for cartilage. These compositions are useful for repairing and supporting tissue which require some degree of structure, for example, in reconstruction of the nose, ear, knee, larynx, tracheal rings, and joint surfaces.
- a reinforcing mesh (e.g., nylon or the like) may optionally be incorporated to increase structural integrity.
- compositions of the invention containing cytokines and growth factors are particularly suited for sustained administration, as in the case of wound healing promotion.
- Osteoinductive factors and cofactors including TGF-S
- bone morphogenic protein BMP
- Compositions provided in the form of a membrane may be used to wrap or coat transplanted organs ⁇ in order to suppress rejection and induce improved tissue growth.
- organs may be coated for transplantation using a crosslinking reaction mixture of growth factor-polymer conjugates and collagen.
- antiviral and antitumor factors such as TNF, interferons, CSFs, TGF-J, and the like for their pharmaceutical activities.
- composition used will depend upon the severity of the condition being treated, the amount of factor incorporated in the composition, the rate of delivery desired, and the like. However, these parameters may easily be determined by routine experimentation, for example, by preparing a model composition following the examples below, and assaying the release rate of active compound in a suitable experimental model.
- the PEG-glutarate is then dissolved in dimethylformamide (DMF, 200 mL) at 37°C, and N- hydroxysuccinimide (10% molar xs) added.
- DMF dimethylformamide
- N- hydroxysuccinimide 10% molar xs
- the solution is cooled to 0°C, and an equivalent amount of dicyclohexylcarbodiimide added in DMF solution (10 mL) .
- the mixture is left at room temperature for 24 hours, and then filtered.
- Cold benzene (100 mL) is then added, and the PEG-succinimidyl glutarate (PEG-SG) precipitated by adding petroleum ether (200 mL) at 0°C.
- the precipitate is collected on a sintered glass filter. Dissolution in benzene, followed by precipitation with petroleum ether is repeated three times to provide "activated" PEG (PEG- SG) .
- Vitrogen 100 ® collagen in solution 400 mL, 1.2 g collagen, 0.004 mmol
- 0.2 M phosphate buffer 44 mL
- a three ⁇ fold molar excess of SG-PEG (6.00 g, 1.2 mmol) was dissolved in water for injection (40 mL) and sterile- filtered.
- the SG-PEG solution was then added to the collagen solution, and the mixture allowed to stand at 17-22°C for about 15 hours.
- the solution was then centrifuged, and the resulting pellet (25 g) of reconstituted fibrils collected and washed with phosphate-buffered saline (PBS, 3X 400 mL) to remove residual PEG.
- PBS phosphate-buffered saline
- the resulting material may be diluted with PBS to provide a dispersion having a concentration of 20.5 mg/mL collagen-PEG.
- B Similarly, proceeding as in part (A) above but substituting polypropylene glycol and POE-POP block polymers for polyethylene glycol, the corresponding collagen-PPG and collagen-POE-POP compositions are prepared.
- the PEG-diglutarate is then dissolved in DMF (200 mL) at 37°C, and N-hydroxysuccinimide (10% molar xs) added. The solution is cooled to 0°C, and an equivalent amount of dicyclohexylcarbodiimide added in DMF solution (10 mL) . The mixture is left at room temperature for 24 hours, and then filtered. Cold benzene (100 mL) is then added, and the PEG-di (succinimidyl glutarate) (dPEG-SG) precipitated by adding petroleum ether (200 mL) at 0°C. The precipitate is collected on a sintered glass filter. Dissolution in benzene, followed by precipitation with petroleum ether is repeated three times to provide "activated" dPEG (dPEG*) .
- Vitrogen 100 ® collagen in solution 400 mL, 1.2 g collagen, 0.004 mmol
- 0.2 M phosphate buffer 44 mL
- a three- fold molar excess of dPEG* (6.00 g, 1.2 mmol) was dissolved in water for injection (40 mL) and sterile- filtered.
- the dPEG* solution was then added to the collagen solution, agitated, and the mixture allowed to stand at 17-22°C for about 15 hours.
- the solution was then centrifuged, and the resulting pellet of reconstituted fibrils collected and washed with PBS (3X 400 mL) to remove residual dPEG*.
- the pellet was then placed in a syringe fitted with a Luer lock hub connected to a second syringe, and was passed between the syringes until homogeneous.
- the resulting material is a microgel or a particulate suspension of random size fibrils in solution (microgel conjugate) .
- the material is a smooth, pliable, rubbery mass, with a shiny appearance.
- NFC cartilage composition was prepared by mixing dPEG* solution (0.6 g, pH 3) with collagen in solution (33.8 mg/mL, pH 2). The mixture was passed between two syringes joined by a Luer lock connector to form a homogenous solution. A solution of dPEG* (20% w/v) in a neutralizing buffer was then added to result in a substantially non-fibrillar collagen (NFC) cartilage material. The resulting product contained approximately 1-40% fibrillar collagen.
- FC cartilage cartilaginoid fibrillar collagen-polymer conjugate
- ZCI Zyderm ® Collagen Implant
- GAX glutaraldehyde-crosslinked fibrillar collagen
- This assay measured the force required to extrude the test composition through a 30 gauge needle. The results can be graphed to show that force required (in Newtons) versus plunger travel allowed for a smooth extrusion of ZCI, requiring a force of about 20-30 Newtons. However, if they are graphed as regards GAX, it will show GAX was not extruded smoothly, as shown by the "spiking" exhibited in the force trace. During certain parts of the extrusion, GAX required about 10-15 N for extrusion. In contrast, collagen-mPEG demonstrated a very low extrusion force (8-10 N) , with little or no spiking.
- Intrusion is a measure of the tendency of a composition to "finger" or channel into a porous bed, rather than remaining in a compact mass. Low intrusion is preferred in augmentation of soft tissue, so that the injected implant does not diffuse through the dermis and remains in place.
- a 1 mL syringe fitted with a 30 gauge needle was half-filled with silicon carbide particles (60 mesh) , simulating human dermis.
- the upper half of the syringe was filled with 0.5 mL test composition (GAX, ZCI, or collagen-mPEG) at 35 mg/mL.
- the plunger was then fitted, and depressed. On depression, ZCI appeared at the needle, demonstrating intrusion through the silicon carbide bed.
- Syringes filled with GAX or collagen-mPEG of the invention did not pass collagen, instead releasing only buffer, demonstrating no intrudability.
- each composition exhibiting nonhelical character was measured using sensitivity to digestion with trypsin.
- Samples were treated with the protease trypsin, which is capable of attacking only fragmented portions of the collagen protein.
- the extent of hydrolysis is measured by fluorescamine assay for solubilized peptides, and the results are expressed as percentage non-helical collagen.
- the percentage of non ⁇ helical collagen was measured 30 minutes after the beginning of the digestion period. The results indicated that ZCI was 3-10% sensitive, GAX was 1-2% sensitive, and collagen-mPEG was about 1% sensitive. Sensitivity to trypsin may also correlate to sensitivity to endogenous proteases following implantation.
- the number of free lysines per mole was determined for each composition using TNBS to quantify reactive epsilon amino groups.
- ZCI exhibited about 30 lysines per (single helix) molecule (K/m)
- GAX exhibited 26- 27 K/m
- collagen-mPEG 21-26 K/m was determined for each composition using TNBS to quantify reactive epsilon amino groups.
- a collagen-dPEG conjugate prepared as described in Example IC was characterized using differential scanning calorimetry (DSC) . This test is a measure of the transition temperature during fragmentation of the collagen molecule at a microscopic level. A lowering of the transition temperature indicates an increase in fragmentation in a manner similar to that measured by trypsin sensitivity.
- DSC differential scanning calorimetry
- the collagen-dPEG conjugate showed a single denaturational transition at 56°C by DSC, which is similar to the typical melting point of the collagen-PEG conjugate prepared in Example 1A.
- ZCI has a melting temperature of 45-53°C, with multiple denaturational transitions
- GAX has a melting temperature of 67-70°C, with a single denaturational transition.
- Example 2A The extrusion test described in Example 2A could not be used to characterize the collagen-dPEG conjugate because the material was not extrudable through a 30 gauge needle.
- Example 3 (Immunogenicity)
- A Non-crosslinked PEG-Collagen: This experiment was conducted to demonstrate the relative immunogenicity of a collagen-mPEG preparation of the invention versus a commercially available bovine collagen formulation prepared from essentially the same source material, and having a similar consistency. As both collagen preparations were prepared using atelopeptide collagen (which is only weakly immunogenic) , the preparations were formulated with either complete Freund's adjuvant (CFA) or incomplete Freund's adjuvant (IFA) , to enhance the immune response.
- CFA complete Freund's adjuvant
- IFA incomplete Freund's adjuvant
- Collagen-mPEG was prepared as in Example 1A above.
- Male Hartley guinea pigs (11) were anesthetized and bled by heart puncture for pre-immunization serologic evaluation.
- Five animals were treated with two 0.1 mL intramuscular injections of Zyderm ® Collagen Implant (ZCI) emulsified in CFA (1:9) in the left and right thighs.
- ZCI Zyderm ® Collagen Implant
- Another five animals were treated in the same fashion, using collagen-PEG (35 mg/mL) emulsified in CFA.
- One animal was treated with collagen-PEG in IFA.
- At day 14 following immunization all animals were again bled by heart puncture, and serum obtained for antibody titer determination (using ELISA) . Serology was again performed at day 30.
- each animal was challenged intradermally with both ZCI and collagen-PEG (0.1 mL of each, one on each flank) .
- DTH Delayed-type hypersensitivity
- Erythema was essentially the same for all animals. Histological studies showed that both materials exhibited comparable intrusion, fingering into the dermis and subcutaneous space. Sites of intradermal challenge with ZCI in ZCI-immunized animals exhibited the most extensive inflammatory response, including a cellular infiltrate of lymphohistiocytic elements with eosinophils and occasional giant cells. Two of the implant sites demonstrated an erosive inflammation of the overlying epidermis and eschar formation. Sites of intradermal challenge with collagen-mPEG in ZCI-immunized animals exhibited only a moderate associated inflammatory infiltrate, with a marked reduction in acute cells and lymphoid elements.
- Example ID Collagen-dPEG conjugates were prepared as in Example ID. The samples were implanted in the dorsal subcutis and as cranial onlays in rats. After implantation for 30 days in the subcutis, NFC cartilage and NFC-FC cartilage materials had a homogeneous microfibrillar structure. Mild colonization by connective tissue cells occurred at the periphery of the NFC-FC cartilage samples, and mild capsule formation was present. No colonization had occurred with the NFC cartilage material and mild capsule formation was present. FC cartilage had a very fibrous structure with mild but frequently deep colonization by connective tissue cells and sparse numbers of adipocytes. Trace amounts of capsule were present in limited areas of the FC cartilage samples. NFC cartilage materials tended to retain their pre-implantation shape, with sharply defined edges, while the NFC-FC cartilage samples tended to flatten over time and develop rounded profiles.
- each of the materials When implanted as cranial onlays, the appearance of each of the materials was similar to that in the subcutis except that the samples tended to become anchored to the skull via integration of the capsule or surrounding loose connective tissue with the periosteum.
- Example 4 (In situ Crosslinking) A dPEG solution was prepared as described in Example IC above. The following samples were then prepared: (1) 5 mg dPEG in 80 ⁇ L water, mixed with 0.5 mL fibrillar collagen (35 mg/mL) , to a final dPEG concentration of 1% by volume; (2) 15 mg dPEG in 80 ⁇ L water, mixed with 0.5 mL fibrillar collagen (35 mg/mL), to a final dPEG concentration of 3% by volume;
- the dPEG solutions of Samples 1, 2, 4, and 5 were placed in a 1 mL syringe equipped with a Luer lock fitting and connector, and joined to another syringe containing the collagen material. The solutions were mixed by passing the liquids back and forth between the syringes several times to form the homogeneous reaction mixture.
- the syringe connector was then removed and replaced with a 27 gauge needle, and approximately 50 ⁇ L of the reaction mixture was injected intradermally into each of 20 guinea pigs. Samples 3, 6, and 7 were similarly administered through a 27 gauge needle. At intervals up to 30 days following injection, the treatment sites were harvested and studied histologically.
- Samples 1 and 2 displayed wide dispersion with an intermediate degree of interdigitation with dermal collagen fibers. Colonization by connective tissue cells was moderate, and a trace of round cell infiltrate with eosinophils was seen. Samples 3, 4, and 5 were highly dispersed and finely interdigitated with dermal collagen fibers. Colonization was mild to moderate, and trace levels of round cell infiltration were seen.
- Example 5 Coating of Implants
- a collagen-dPEG reaction mixture was prepared as described in Example IC above.
- a titanium implant was dipped into the reaction mixture approximately 20 minutes after crosslinking was initiated. The implant was then allowed to finish crosslinking and dried overnight.
- composition based on fibrillar atelopeptide collagen was prepared as in part A above, but limiting TGF-Jl/dPEG* reaction time to 2 minutes, and substituting 7 mg of fibrillar collagen (precipitated from collagen in solution within 2 minutes prior to use) for collagen in solution.
- a composition containing dPEG-crosslinked collagen and free TGF-01 was prepared as follows: A solution of dPEG* (4 mg) in CH 2 C1 2 (100 ⁇ L) was added to 2.5 mL of CIS (3 mg/mL atelopeptide nonfibrillar collagen) , and the resulting mixture allowed to incubate overnight at ambient temperature. The pellet which formed was washed to remove unreacted dPEG*, and 25 ⁇ g of TGF-jSl mixed in to provide collagen-dPEG + TGF-01.
- D The degree of TGF-01 binding was determined as follows:
- composition prepared in parts A-C above was washed six times with 0.5 mL of buffer (0.02 M phosphate buffer, 0.1% BSA) by vigorous, vortexing followed by centrifugation in order to remove non-bound TGF- ⁇ l. The pellet and supernatants were collected at each time of washing, and were counted. The results can be graphed to demonstrate that the TGF-J1 in the simple mixture is quantitatively released within about 6. washings, while approximately 40% of the TGF-01 is retained in the compositions of part B and 50% is retained in the compositions of part A.
- compositions prepared according to part A (CIS-dPEG- TGF-jSl) (TGF-jSl/dPEG* reaction time of 12 minutes) and part C (CIS-dPEG + TGF-3D were prepared, as well as a control prepared according to part C without TGF-/S1 (CIS- dPEG) .
- the samples were washed in PBS/BSA eight times as described in part D, then washed an additional three times in fetal bovine serum (Gibco) at 37°C. This washing protocol resulted in visually detectable material loss, so remaining TGF-S1 content was determined by
- TGF- ⁇ l activity was then assayed by ELISA. The results are shown in Table 2 below.
- a formulation suitable for implantation by injection was prepared by suspending collagen-PEG in sterile water for injection, at 35 mg/mL. The characteristics of the resulting formulation are described in Example 2 above.
- a formulation useful for repair of stress- bearing bone defects may . be prepared by mixing collagen-PEG of the invention with a suitable particulate, insoluble component.
- the insoluble component may be fibrillar crosslinked collagen, gelatin beads, polytetrafluoroethylene beads, silicone rubber beads, hydrogel beads, silicon carbide beads, mineral beads, or glass beads, and is preferably a calcium mineral, for example hydroxyapatite and/or tricalcium phosphate.
- Solid formulations were prepared by mixing Zyderm ® II Collagen (65 mg/mL collagen) or collagen-mPEG (63 mg/mL) with particulate hydroxyapatite and tricalcium phosphate (HA+TCP) and air drying to form a solid block containing 65% HA by weight.
- blocks were heat-treated by heating at 75°C for 10 hours. The resulting blocks were hydrated in 0.13 M saline for 12 hours prior to testing.
- Z- HA Zyderm ® -HA+TCP
- PC-HA PEG-collagen-HA+TCP
- implant compositions with collagen-polymer which are substantially stronger than compositions employing the same amount of non- conjugated collagen, or may reduce the amount of collagen-polymer employed to form a composition of equal strength.
- Example 8 Crosslinking of Hyaluronic Acid With Difunctional SC-PEG
- sodium hyaluronate obtained from LifeCore Biomedical
- PBS sodium hyaluronate
- 5 gram of sodium hyaluronate/PBS solution was mixed with 50 mg of difunctional SC-PEG in 0.5 ml of PBS using syringe-to-syringe mixing.
- the resulting material was extruded from the syringe into a petri dish and incubated at 37°C for 16 hours. The material was then allowed to cool at room temperature for 8 hours. After 24 hours, the material had formed a crosslinked gel.
- Hyaluronic acid without S-PEG was used as a control in this experiment. After the same incubation period, the control was still liquid and runny.
- the needle end was snipped off of a standard 4.5 mm inner diameter syringe containing Zyderm ® I Collagen (35 mg/ml, available from Collagen Corporation, Palo Alto, California) .
- Zyderm ® I Collagen 35 mg/ml, available from Collagen Corporation, Palo Alto, California
- the collagen cylinder was placed in a petri dish and immersed in a 10% solution of difunctional S-PEG (1.0 g of difunctional S-PEG in 10 ml of PBS) .
- the collagen cylinder was allowed to incubate in the 10% S-PEG solution at room temperature.
- the crosslinking reaction occurs as the PEG diffuses from the outside towards the inside of the collagen cylinder.
- After 20 - 30 minutes of incubation in the S-PEG solution the outside of the collagen cylinder had been crosslinked, while the inside remained non-crosslinked.
- the collagen cylinder was removed from the crosslinker solution.
- the inner, non-crosslinked collagen could easily be squeezed out from the outer crosslinked shell using manual pressure, leaving a hollow tube of PEG-crosslinked collagen.
- the hollow tube was then returned to the 10% S-PEG solution and incubated overnight at 37°C in order to complete the crosslinking process.
- the outer diameter of the hollow PEG-collagen tube can be varied by varying the size of the collagen cylinder starting material.
- the inner diameter of the tube can be increased by decreasing the length of time for the initial incubation of the collagen cylinder in the PEG solution. Conversely, the inner diameter of the tube can be made smaller by increasing the initial incubation period.
- Example 10 (Preparation of Pleated Collagen-Polymer Tube) A smooth collagen-polymer tube was prepared according to the method described in Example 9. While still wet, the tube was slipped over the plunger of the same syringe that had originally contained the Zyderm ® I Collagen starting material. The tube fit snugly over the syringe plunger. The PEG-collagen tube was then pushed down along the axis of the syringe plunger, forming pleats or ribs in the wet tubing, so that the pleated tube was now approximately half the length of the original smooth tube. While still on the syringe plunger, the pleated PEG- collagen tube was dried under the fume hood at room temperature. After 24 hours, the dried pleated tube was pushed off the syringe plunger. The tube retained its pleated shape after removal from the syringe plunger.
- the pleated PEG-collagen tube was then placed in a petri dish containing water.
- the tube retained its pleated shape following rehydration.
- the PEG-collagen material was extruded using an 18-gauge needle into TFE tubing (1.5 mm outer diameter, 1.3 mm inner diameter) . (It was necessary to add a certain amount of PEG to provide a starting material with greater structural integrity than straight Zyderm ® I Collagen in order to maintain the shape of the small-diameter cylinder.)
- the tubing was sliced open and the solid cylinder of PEG-collagen was peeled out of the tubing.
- the PEG-collagen cylinder was then placed in a petri dish containing 5 cc of a 10% solution of difunctional S-PEG.
- the crosslinking reaction occurs as the PEG diffuses from the outside towards the inside of the collagen cylinder.
- the inside of the cylinder was pushed out using a 1 mm diameter mandrel, resulting in a hollow, smooth PEG-collagen tube.
- the PEG-collagen tube was then pushed down along the axis of the mandrel, forming pleats or ribs in the wet tubing, so that the pleated tube was now approximately half the length of the original smooth tube.
- the pleated PEG-collagen tube was dried under the fume hood at room temperature. After 24 hours, the dried pleated tube was pushed off the mandrel. The tube retained its pleated shape after removal from the mandrel.
- the pleated PEG-collagen tube was then placed in a petri dish containing water.
- the tube retained its pleated shape following rehydration.
- PEG-collagen tubes of different diameters can be prepared by using different sizes of TFE tubing and varying the time for the crosslinking reaction to occur.
- the outer tubing was pulled off and the inner tubing with the PEG-collagen shell around it was incubated at 37 ⁇ C for an additional 2 hours.
- the thin PEG-collagen shell was then carefully pushed off of the inner TFE tubing.
- the resulting PEG- collagen tube was clear and cellophane-like in consistency.
- the PEG-collagen tube was then placed in water to rehydrate. Although the tube was very thin and had a small diameter, water could be injected through it.
- the thickness of the tube wall and the inner diameter of the collagen-polymer tube can be varied by varying the size of the inner and outer TFE tubes used to mold the collagen-polymer material. Thin-walled tubes produced according to the method described above may be especially suited for use as nerve guide tubes to facilitate nerve regeneration.
- Non-crosslinked collagen strings were produced as a control by mixing 5 ml of Zyderm ® I Collagen with 0.5 ml of PBS. The method described above was used to produce strings of two different diameters. Diameter, length, and weight of the strings were measured in the fresh (wet) , dehydrated, and rehydrated states. Results of these measurements are presented graphically in Figure 1 and in the table shown in figure 14. Because the non-crosslinked strings do not contain the hydrophilic PEG, they were not able to take up water and rehydrate. Therefore, no measurements were obtained for these strings in the rehydrated state. The strings retained all of their original length and nearly all of their original diameter and weight upon rehydration.
- the bar graphs in Figures 2-5 illustrate the large standard deviation and variability of the rheological measurements obtained for the non-crosslinked strings, showing the non-homogeneity of the non-crosslinked materials.
- the consistent results obtained with the PEG- crosslinked strings show that PEG crosslinking imparts homogeneity, as well as greater mechanical strength and elasticity, to the collagen material.
- Example 14 (Preparation of Coiled Strings) A small-diameter crosslinked collagen string was produced as described in Example 13 by injecting collagen in a 1% solution of difunctional S-PEG through an 18- gauge needle into TFE tubing (0.9 mm outer diameter, 0.6 mm inner diameter) .
- the wet string was coiled around a second piece of TFE tubing having an outer diameter of 1.5 mm.
- the coiled string was dried on the tubing for 2 days at room temperature under the fume hood.
- the dehydrated PEG-collagen coil was pushed off the tubing.
- the coiled string in its dried state was manually pulled straight.
- the now-straight string was immersed in water and quickly returned to its coiled shape upon rehydration.
- the coiled wet string was removed from the water bath, pulled and dried straight under tension.
- the straight dried string was again immersed in water and again returned to its original coiled shape upon rehydration.
- the collagen-polymer coils can be pulled straight to facilitate delivery through a needle or catheter. They are especially useful in the treatment of aneurysms because of their ability to rehydrate to the coil shape and expand to fill the void.
- the above example illustrates the "memory" of the collagen-polymer material. Upon rehydration, the material returns to the original shape in which it was first dried.
- a 10% solution of activated difunctional SG-PEG was prepared by diluting 100 mg of powdered difunctional SG- PEG (3400 dalton MW) in 1 ml of phosphate buffered saline (PBS) .
- PBS phosphate buffered saline
- One (1) ml of the 10% difunctional SG-PEG solution was mixed with 9 ml of Zyderm ® I Collagen (Z-I, 35 mg/ml) to achieve a final PEG concentration of 1%.
- the collagen and crosslinker solution were placed in 10- ml syringes and mixed using syringe-to-syringe mixing.
- Zyderm ® II Collagen (Z-II, 65 mg/ml) was crosslinked with difunctional SG-PEG using the same method described above.
- the syringes containing the Z-I-PEG and Z-II-PEG composites were incubated at 37°C for 16 hours and formed polymerized gels.
- each of the two syringes was cut off and the gels pushed out of the barrels of the syringes using the respective syringe plunger.
- the solid gels were then sliced into disks of 2 mm thickness, dehydrated, and then rehydrated. Diameter, thickness and weight of the disks were measured in the fresh (wet) , dehydrated, and rehydrated states. Results of these measurements are presented in the table shown in figure 16.
- crosslinked collagen disks (at both collagen concentrations) regained nearly all of their original dimensions upon rehydration.
- Lidocaine-free Zyderm ® II Collagen (65 mg/ml, available from Collagen Corporation, Palo Alto, CA) was diluted to 32.5 mg/ml using sterile-filtered phosphate buffered saline (PBS) .
- PBS phosphate buffered saline
- a 10% solution of activated difunctional S-PEG was prepared by diluting powdered difunctional S-PEG in sterile-filtered PBS.
- One hundred (100) ⁇ l of the S-PEG was added to 900 ⁇ l of the collagen to achieve a final S-PEG concentration of 1%.
- the S-PEG solution and collagen were placed in 3-ml syringes and mixed using syringe-to-syringe mixing.
- Platinum wires (#1, available from Target Therapeutics, Santa Clara, CA) coiled to a diameter of 0.25 mm on mandrels (inner wires to keep coils straight) were placed inside ultra micro pipet tips (0.5 - 10 ⁇ l) , one coil per tip, so that the mandrel passed through both the narrow and wide openings of the pipet tip.
- the S-PEG - collagen mixture in the syringe was injected into each of the pipet tips using a 22-gauge needle.
- the pipet tips containing the coils were placed in a petri dish and allowed to incubate at 37°C for twenty- four hours.
- the tips containing the coils were placed under the laminar flow hood at room temperature for six days.
- the coated coils were then removed from the pipet tips. The coatings will not adhere well to the coils if drying time is not adequate.
- the coated coils can be delivered using a Tracker ® 325i catheter (Target Therapeutics) or other similar type catheter.
- Example 17 (Coating of Platinum Wires) Lidocaine-free Zyderm ® II Collagen was diluted to 32.5 mg/ml using sterile-filtered PBS. A 10% solution of activated difunctional S-PEG was prepared by diluting powdered difunctional S-PEG in sterile-filtered PBS. One hundred (100) ⁇ l of the S-PEG solution was added to 900 ⁇ l of the collagen to achieve a final S-PEG concentration of 1%. The S-PEG solution and collagen were placed in 3-ml syringes and mixed using syringe-to- syringe mixing.
- the coated coils can be delivered via catheter.
- a 3-ml sample of the pellet was acidified by adding 0.5 ml of 0.1 M hydrochloric acid (HCI). The resulting material was very opaque and fibrillar at acidic pH (4 - 5) .
- One-half (0.5) ml of the acidified telopeptide- containing collagen was placed in a mold and 0.25 ml of a 35.7% (wt.%/vol.%) concentration difunctionally activated SG-PEG solution was added.
- the mold containing the collagen and crosslinker solution was incubated overnight at 37°C. Crosslinking occurred as the PEG solution diffused into the collagen gel. Five strips (30 mm x 10 mm x 2 mm thickness) of tightly crosslinked telopeptide- containing collagen gel were obtained.
- Zyderm ® II Collagen (65 mg/ml concentration; available from Collagen Corporation, Palo Alto, CA) was diluted to a concentration of 40 mg/ml using phosphate buffered saline (PBS) .
- PBS phosphate buffered saline
- Three (3) ml of the resulting 40 mg/ml atelopeptide collagen was acidified and crosslinked with difunctionally activated SG-PEG according to the method described above. Five strips (30 mm x 10 mm x 2 mm thickness) of tightly crosslinked atelopeptide collagen were obtained.
- telopeptide-containing collagen pellet Three (3) ml of telopeptide-containing collagen pellet and 3 ml of Zyderm II Collagen were acidified as described.above. One-half (0.5) ml of each of the two acidified collagen materials were placed in molds. The molds containing the acidified telopeptide-containing and atelopeptide collagen were incubated at 37°C overnight. After incubation at 37°C overnight, a moderately crosslinked telopeptide-containing collagen gel was obtained. The atelopeptide collagen failed to produce any crosslinked gel.
- DSC Differential Scanning Calorimetry
- DSC Differential scanning calorimetry
- Non-crosslinked atelopeptide collagen shows two temperature transition peaks, one at approximately 46°C and a second at approximately 54°C.
- Non-crosslinked telopeptide-containing collagen also has two temperature transitions, one occurring at 49°C and the second occurring at 57°C.
- the peaks for the telopeptide- containing collagen are broader than those for the atelopeptide collagen because the telopeptide-containing collagen contains indigenous crosslinks that are removed during enzyme treatment and are not present in the resulting purified atelopeptide collagen.
- telopeptide collagen When atelopeptide collagen is crosslinked with difunctionally activated S-PEG, "the transition temperature is increased to approximately 58°C and appears as a single peak.
- the transition temperature When telopeptide-containing collagen is crosslinked with difunctionally activated S- PEG, the transition temperature is increased to approximately 66°C and consists of a single transition peak which is somewhat broader than the comparable transition peak for the PEG-crosslinked atelopeptide collagen.
- the broad peak obtained for the crosslinked telopeptide-containing collagen is likely due to increased heterogeneity as a result of the indigenous crosslinks, as compared with the crosslinked atelopeptide collagen, which is more homogeneous as a res ⁇ lt of enzyme treatment and purification.
- Non-crosslinked and PEG-crosslinked telopeptide- containing collagen and atelopeptide collagen were tested for tensile strength (N) , tensile stress (N/mm 2 ) , strain ( ⁇ L/L) , and Young's Modulus (N/mm 2 ). Results of the rheological testing are presented in the table shown in figure 17.
- Tensile stress (N/mm 2 ) is a measure of the force at failure (breakage) of a material as a function of its cross-sectional area.
- Strain ( ⁇ length/length) is a measure of the elasticity of a material (how much it will stretch under tension) .
- Young's Modulus (N/mm 2 ) is calculated by dividing stress by strain and is known as the rheological "fingerprint" of a particular material.
- the crosslinked telopeptide-containing collagen is significantly stronger than the crosslinked atelopeptide material.
- non-crosslinked atelopeptide collagen is not a gel, it fell apart when immersed in water and, therefore, no rehydrated weight value could be obtained.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Organic Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Surgery (AREA)
- Vascular Medicine (AREA)
- Heart & Thoracic Surgery (AREA)
- Polymers & Plastics (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Composite Materials (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
Description
Claims
Applications Claiming Priority (13)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US907518 | 1992-07-02 | ||
US07/907,518 US5324775A (en) | 1988-11-21 | 1992-07-02 | Biologically inert, biocompatible-polymer conjugates |
US922541 | 1992-07-30 | ||
US07/922,541 US5328955A (en) | 1988-11-21 | 1992-07-30 | Collagen-polymer conjugates |
US98493392A | 1992-12-02 | 1992-12-02 | |
US07/985,680 US5292802A (en) | 1988-11-21 | 1992-12-02 | Collagen-polymer tubes for use in vascular surgery |
US07/984,197 US5308889A (en) | 1988-11-21 | 1992-12-02 | Dehydrated collagen-polymer strings |
US984933 | 1992-12-02 | ||
US984197 | 1992-12-02 | ||
US2503293A | 1993-03-02 | 1993-03-02 | |
US25032 | 1993-03-02 | ||
PCT/US1993/006292 WO1994001483A1 (en) | 1992-07-02 | 1993-07-01 | Biocompatible polymer conjugates |
US985680 | 2001-11-05 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0648239A1 true EP0648239A1 (en) | 1995-04-19 |
EP0648239A4 EP0648239A4 (en) | 1995-09-27 |
Family
ID=27556046
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP93916926A Withdrawn EP0648239A4 (en) | 1992-07-02 | 1993-07-01 | Biocompatible polymer conjugates. |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0648239A4 (en) |
JP (1) | JPH08502082A (en) |
AU (1) | AU677789B2 (en) |
WO (1) | WO1994001483A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0713707A1 (en) | 1994-11-23 | 1996-05-29 | Collagen Corporation | In situ crosslinkable, injectable collagen composition for tissue augmention |
Families Citing this family (59)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5936035A (en) * | 1988-11-21 | 1999-08-10 | Cohesion Technologies, Inc. | Biocompatible adhesive compositions |
US5565519A (en) * | 1988-11-21 | 1996-10-15 | Collagen Corporation | Clear, chemically modified collagen-synthetic polymer conjugates for ophthalmic applications |
US5643464A (en) * | 1988-11-21 | 1997-07-01 | Collagen Corporation | Process for preparing a sterile, dry crosslinking agent |
US5475052A (en) * | 1988-11-21 | 1995-12-12 | Collagen Corporation | Collagen-synthetic polymer matrices prepared using a multiple step reaction |
US5510418A (en) * | 1988-11-21 | 1996-04-23 | Collagen Corporation | Glycosaminoglycan-synthetic polymer conjugates |
EP0661045B1 (en) | 1992-09-18 | 2002-07-03 | Yamanouchi Pharmaceutical Co. Ltd. | Sustained-release hydrogel preparation |
US5548064A (en) * | 1993-05-24 | 1996-08-20 | Biotech Australia Pty Limited | Vitamin B12 conjugates with EPO, analogues thereof and pharmaceutical compositions |
CA2140053C (en) * | 1994-02-09 | 2000-04-04 | Joel S. Rosenblatt | Collagen-based injectable drug delivery system and its use |
AU1014695A (en) * | 1994-02-17 | 1995-08-24 | Collagen Corporation | Collagen-synthetic polymer conjugates having controlled fiber size distributions |
WO1995026761A1 (en) * | 1994-04-04 | 1995-10-12 | Collagen Corporation | Cell-gels |
CA2151547C (en) * | 1994-06-10 | 2010-10-12 | Elliott A. Gruskin | Recombinant chimeric proteins and methods of use thereof |
US6962979B1 (en) | 1995-03-14 | 2005-11-08 | Cohesion Technologies, Inc. | Crosslinkable biomaterial compositions containing hydrophobic and hydrophilic crosslinking agents |
CA2165728A1 (en) * | 1995-03-14 | 1996-09-15 | Woonza M. Rhee | Use of hydrophobic crosslinking agents to prepare crosslinked biomaterial compositions |
DE19514087A1 (en) * | 1995-04-13 | 1996-10-17 | Deutsches Krebsforsch | Conjugate of an active ingredient, a polyether and possibly a native protein that is not considered foreign to the body |
AU722322B2 (en) * | 1995-06-07 | 2000-07-27 | Organogenesis Inc. | Reconstituted collagen fiber segment compositions and methods of preparation thereof |
US5874500A (en) * | 1995-12-18 | 1999-02-23 | Cohesion Technologies, Inc. | Crosslinked polymer compositions and methods for their use |
US5776193A (en) | 1995-10-16 | 1998-07-07 | Orquest, Inc. | Bone grafting matrix |
JPH09143093A (en) | 1995-11-17 | 1997-06-03 | Hoechst Japan Ltd | Cartilage/bone-inductive restoring material |
US5752974A (en) * | 1995-12-18 | 1998-05-19 | Collagen Corporation | Injectable or implantable biomaterials for filling or blocking lumens and voids of the body |
WO1997032591A1 (en) * | 1996-03-05 | 1997-09-12 | Orquest, Inc. | Method of promoting bone growth with hyaluronic acid and growth factors |
US5855615A (en) * | 1996-06-07 | 1999-01-05 | Menlo Care, Inc. | Controller expansion sphincter augmentation media |
TW555765B (en) | 1996-07-09 | 2003-10-01 | Amgen Inc | Low molecular weight soluble tumor necrosis factor type-I and type-II proteins |
AU6019898A (en) | 1997-01-09 | 1998-08-03 | Cohesion Technologies, Inc. | Devices for tissue repair and methods for preparation and use thereof |
GB9722604D0 (en) * | 1997-10-28 | 1997-12-24 | Cancer Res Campaign Tech | Heparin-binding growth factor derivatives |
JP2002534165A (en) * | 1998-12-31 | 2002-10-15 | アンジオテック ファーマシューティカルズ,インコーポレイテッド | Stent graft with bioactive coating |
EP1316323A1 (en) * | 1998-12-31 | 2003-06-04 | Angiotech Pharmaceuticals, Inc. | Stent grafts with bioactive coatings |
FR2790475B1 (en) | 1999-03-02 | 2003-01-24 | Flamel Tech Sa | COLLAGENIC PEPTIDES MODIFIED BY GRAFTING OF MERCAPTO FUNCTIONS, ONE OF THEIR PROCESSES AND THEIR APPLICATIONS AS BIOMATERIALS |
AU6357900A (en) * | 1999-07-20 | 2001-02-05 | Amgen, Inc. | Hyaluronic acid-protein conjugates, pharmaceutical compositions and related methods |
US6592794B1 (en) | 1999-09-28 | 2003-07-15 | Organogenesis Inc. | Process of making bioengineered collagen fibrils |
WO2001032850A1 (en) * | 1999-10-29 | 2001-05-10 | Min Hu | Surface treatment with polypeptides to improve fibroblast adhesion to hyaluronan |
WO2001045595A2 (en) | 1999-12-17 | 2001-06-28 | Cartificial A/S | A prosthetic device |
SG98393A1 (en) | 2000-05-19 | 2003-09-19 | Inst Materials Research & Eng | Injectable drug delivery systems with cyclodextrin-polymer based hydrogels |
WO2002016557A2 (en) * | 2000-08-21 | 2002-02-28 | Rice University | Tissue engineering scaffolds promoting matrix protein production |
WO2003072156A1 (en) * | 2002-02-28 | 2003-09-04 | Eidgenössische Technische Hochschule Zürich Oberflächentechnik | Biomedical implant surfaces with osteogenic properties |
DE10209821A1 (en) | 2002-03-06 | 2003-09-25 | Biotechnologie Ges Mittelhesse | Coupling of proteins to a modified polysaccharide |
JP2004173941A (en) * | 2002-11-27 | 2004-06-24 | Olympus Corp | Calcium gradient material and its manufacturing method |
CA2511486A1 (en) * | 2002-12-30 | 2004-07-22 | Angiotech International Ag | Tissue reactive compounds and compositions and uses thereof |
WO2004060346A2 (en) | 2002-12-30 | 2004-07-22 | Angiotech International Ag | Drug delivery from rapid gelling polymer composition |
FR2850282B1 (en) | 2003-01-27 | 2007-04-06 | Jerome Asius | INJECTABLE IMPLANT BASED ON CERAMIC FOR THE FILLING OF WRINKLES, CUTANEOUS DEPRESSIONS AND SCARS, AND ITS PREPARATION |
DE10303584A1 (en) * | 2003-01-30 | 2004-08-12 | Stauss, Robert, Dr. | Erythropoietin is applied as a coating material to medical implants to improve the acceptance behavior of relevant body tissues |
JP4669919B2 (en) * | 2003-06-06 | 2011-04-13 | コスモテック株式会社 | Medical composition |
WO2005014655A2 (en) | 2003-08-08 | 2005-02-17 | Fresenius Kabi Deutschland Gmbh | Conjugates of hydroxyalkyl starch and a protein |
AU2005212339B2 (en) | 2004-02-06 | 2010-11-25 | Georgia Tech Research Corporation | Load bearing biocompatible device |
WO2005077013A2 (en) | 2004-02-06 | 2005-08-25 | Georgia Tech Research Corporation | Surface directed cellular attachment |
JP4210231B2 (en) * | 2004-03-25 | 2009-01-14 | 株式会社資生堂 | Cosmetic method for improving skin wrinkles and wrinkle improving tool |
US8303973B2 (en) | 2004-09-17 | 2012-11-06 | Angiotech Pharmaceuticals (Us), Inc. | Multifunctional compounds for forming crosslinked biomaterials and methods of preparation and use |
KR100642220B1 (en) * | 2005-07-12 | 2006-11-03 | 광주과학기술원 | Conjugates of cyclodextrin and poly(oxyethylene) and process for preparation thereof |
JPWO2007083522A1 (en) * | 2006-01-18 | 2009-06-11 | 株式会社ネクスト21 | Medical gel-forming composition, administration device for the composition, and drug release control carrier |
US8795709B2 (en) * | 2006-03-29 | 2014-08-05 | Incept Llc | Superabsorbent, freeze dried hydrogels for medical applications |
BRPI0811784A2 (en) * | 2007-05-23 | 2011-05-10 | Allergan Inc | cross-linked collagen and use thereof |
JP5570988B2 (en) | 2007-08-27 | 2014-08-13 | ラティオファーム ゲーエムベーハー | Liquid preparation of G-CSF conjugate |
US20120122949A1 (en) * | 2008-12-19 | 2012-05-17 | The University Of Tokyo | Ultra-high strength injectable hydrogel and process for producing the same |
CA3048437C (en) | 2011-05-26 | 2022-06-21 | Cartiva, Inc. | Tapered joint implant and related tools |
JP5944902B2 (en) * | 2011-08-05 | 2016-07-05 | 公立大学法人大阪市立大学 | Functional hydrogel |
CA2981061A1 (en) | 2015-03-31 | 2016-10-06 | Cartiva, Inc. | Hydrogel implants with porous materials and methods |
US10758374B2 (en) | 2015-03-31 | 2020-09-01 | Cartiva, Inc. | Carpometacarpal (CMC) implants and methods |
CA2981074C (en) | 2015-04-14 | 2023-03-28 | Cartiva, Inc. | Tooling for creating tapered opening in tissue and related methods |
US20160303281A1 (en) * | 2015-04-17 | 2016-10-20 | Rochal Industries, Llc | Composition and kits for pseudoplastic microgel matrices |
US20220202996A1 (en) * | 2019-05-08 | 2022-06-30 | Seoul National University Hospital | Medical implant surface-modified with functional polypeptide |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3091542A (en) * | 1960-09-20 | 1963-05-28 | Dow Chemical Co | Insolubilization of water-soluble cellulose ethers |
US4487865A (en) * | 1983-12-15 | 1984-12-11 | Biomatrix, Inc. | Polymeric articles modified with hyaluronate |
JPS643116A (en) * | 1987-06-25 | 1989-01-06 | Nippon Oil & Fats Co Ltd | Plaster for patch |
WO1990005755A1 (en) * | 1988-11-21 | 1990-05-31 | Collagen Corporation | Collagen-polymer conjugates |
WO1992004048A2 (en) * | 1990-08-30 | 1992-03-19 | The Biomembrane Institute | Inhibition of metastasis potential and invasiveness by oligosaccharides or oligosaccharide antigens or antibodies |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3949073A (en) * | 1974-11-18 | 1976-04-06 | The Board Of Trustees Of Leland Stanford Junior University | Process for augmenting connective mammalian tissue with in situ polymerizable native collagen solution |
JPS5896026A (en) * | 1981-10-30 | 1983-06-07 | Nippon Chemiphar Co Ltd | Novel urokinase derivative, its preparation and thrombolytic agent containing the same |
US4424208A (en) * | 1982-01-11 | 1984-01-03 | Collagen Corporation | Collagen implant material and method for augmenting soft tissue |
US4496689A (en) * | 1983-12-27 | 1985-01-29 | Miles Laboratories, Inc. | Covalently attached complex of alpha-1-proteinase inhibitor with a water soluble polymer |
US4847325A (en) * | 1988-01-20 | 1989-07-11 | Cetus Corporation | Conjugation of polymer to colony stimulating factor-1 |
-
1993
- 1993-07-01 JP JP6503427A patent/JPH08502082A/en active Pending
- 1993-07-01 WO PCT/US1993/006292 patent/WO1994001483A1/en not_active Application Discontinuation
- 1993-07-01 AU AU46620/93A patent/AU677789B2/en not_active Ceased
- 1993-07-01 EP EP93916926A patent/EP0648239A4/en not_active Withdrawn
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3091542A (en) * | 1960-09-20 | 1963-05-28 | Dow Chemical Co | Insolubilization of water-soluble cellulose ethers |
US4487865A (en) * | 1983-12-15 | 1984-12-11 | Biomatrix, Inc. | Polymeric articles modified with hyaluronate |
JPS643116A (en) * | 1987-06-25 | 1989-01-06 | Nippon Oil & Fats Co Ltd | Plaster for patch |
WO1990005755A1 (en) * | 1988-11-21 | 1990-05-31 | Collagen Corporation | Collagen-polymer conjugates |
WO1992004048A2 (en) * | 1990-08-30 | 1992-03-19 | The Biomembrane Institute | Inhibition of metastasis potential and invasiveness by oligosaccharides or oligosaccharide antigens or antibodies |
Non-Patent Citations (2)
Title |
---|
DATABASE WPI Week 8907 Derwent Publications Ltd., London, GB; AN 51979 & JP-A-64 003 116 (NIPPON OILS & FATS KK) , 6 January 1989 * |
See also references of WO9401483A1 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0713707A1 (en) | 1994-11-23 | 1996-05-29 | Collagen Corporation | In situ crosslinkable, injectable collagen composition for tissue augmention |
Also Published As
Publication number | Publication date |
---|---|
AU4662093A (en) | 1994-01-31 |
AU677789B2 (en) | 1997-05-08 |
JPH08502082A (en) | 1996-03-05 |
EP0648239A4 (en) | 1995-09-27 |
WO1994001483A1 (en) | 1994-01-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU677789B2 (en) | Biocompatible polymer conjugates | |
US5324775A (en) | Biologically inert, biocompatible-polymer conjugates | |
US5264214A (en) | Composition for bone repair | |
US5523348A (en) | Method of preparing collagen-polymer conjugates | |
US5304595A (en) | Collagen-polymer conjugates | |
US5510121A (en) | Glycosaminoglycan-synthetic polymer conjugates | |
JP4283719B2 (en) | Crosslinked polymer composition and method of use thereof | |
AU698233B2 (en) | Collagen-synthetic polymer matrices prepared using multiple step reaction | |
US20040121951A1 (en) | Use of hydrophobic crosslinking agents to prepare crosslinked biomaterial compositions | |
EP0732109A1 (en) | Use of hydrophobic crosslinking agents to prepare crosslinked biomaterial compositions | |
EP0668081A2 (en) | Collagen-synthetic polymer conjugates having controlled fiber size distributions | |
JP2006181389A (en) | Use of hydrophobic cross-linking agent for preparing cross-linked biological material composition | |
JP2008132338A (en) | Use of hydrophobic crosslinking agent to prepare crosslinked biological material composition |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 19950109 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE |
|
A4 | Supplementary search report drawn up and despatched | ||
AK | Designated contracting states |
Kind code of ref document: A4 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE |
|
DA4 | Supplementary search report drawn up and despatched (deleted) | ||
RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: MCCULLOUGH, KIMBERLY Inventor name: BENTZ, HANNE Inventor name: DELUSTRO, FRANK Inventor name: FRIES, LOUIS Inventor name: BURNS, RAMON A. JR. Inventor name: MICHAELS, ALAN, S. Inventor name: WALLACE, DONALD, G. Inventor name: RHEE, WOONZA |
|
RA4 | Supplementary search report drawn up and despatched (corrected) |
Effective date: 19960119 |
|
17Q | First examination report despatched |
Effective date: 19970707 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 19980120 |