EA201990861A1 - Рнк-направляемые модифицирующие нуклеиновые кислоты ферменты и способы их применения - Google Patents

Рнк-направляемые модифицирующие нуклеиновые кислоты ферменты и способы их применения

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EA201990861A1
EA201990861A1 EA201990861A EA201990861A EA201990861A1 EA 201990861 A1 EA201990861 A1 EA 201990861A1 EA 201990861 A EA201990861 A EA 201990861A EA 201990861 A EA201990861 A EA 201990861A EA 201990861 A1 EA201990861 A1 EA 201990861A1
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nucleic acids
casx
acids encoding
proteins
rna
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EA201990861A
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Дженифер А. Дудна
Джилиан Ф. Бэнфилд
Дэвид Бёрнстайн
Лукас Бенджамин Харрингтон
Стивен С. Струтт
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Те Риджентс Оф Те Юниверсити Оф Калифорния
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Publication of EA201990861A1 publication Critical patent/EA201990861A1/ru

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Abstract

В настоящем изобретении предложены белки CasX, нуклеиновые кислоты, кодирующие белки CasX, и модифицированные клетки-хозяева, содержащие белки CasX и/или кодирующие их нуклеиновые кислоты. Белки CasX можно использовать во множестве применений, которые предложены. В настоящем изобретении предложены CasX-направляющие РНК, которые связываются с белками CasX и обеспечивают белкам CasX специфичность последовательностей, нуклеиновые кислоты, кодирующие CasX-направляющие РНК; и модифицированные клетки-хозяева, содержащие CasX-направляющие РНК и/или кодирующие их нуклеиновые кислоты. CasX-направляющие РНК можно использовать во множестве применений, которые предложены. В настоящем изобретении предложены полипептиды Cas9 архей и кодирующие их нуклеиновые кислоты, а также связанные с ними направляющие РНК Cas9 архей и кодирующие их нуклеиновые кислоты.
EA201990861A 2016-09-30 2017-09-28 Рнк-направляемые модифицирующие нуклеиновые кислоты ферменты и способы их применения EA201990861A1 (ru)

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US201662402846P 2016-09-30 2016-09-30
PCT/US2017/054081 WO2018064371A1 (en) 2016-09-30 2017-09-28 Rna-guided nucleic acid modifying enzymes and methods of use thereof

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US (4) US11873504B2 (ru)
EP (1) EP3523426A4 (ru)
JP (2) JP2019532644A (ru)
KR (2) KR20230170126A (ru)
CN (1) CN110023494A (ru)
AU (1) AU2017335890B2 (ru)
BR (1) BR112019006384A2 (ru)
CA (1) CA3038960A1 (ru)
EA (1) EA201990861A1 (ru)
GB (1) GB2569733B (ru)
IL (1) IL265598A (ru)
MX (1) MX2019003674A (ru)
WO (1) WO2018064371A1 (ru)

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