CN216525449U - Multifunctional miniature fluorescent dark field microscopic imaging device - Google Patents
Multifunctional miniature fluorescent dark field microscopic imaging device Download PDFInfo
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- CN216525449U CN216525449U CN202122807307.0U CN202122807307U CN216525449U CN 216525449 U CN216525449 U CN 216525449U CN 202122807307 U CN202122807307 U CN 202122807307U CN 216525449 U CN216525449 U CN 216525449U
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Abstract
A multifunctional miniature fluorescent dark field microscopic imaging device belongs to the technical field of microscopic imaging. The shell body upper end is connected and the light source with the camera adaptor that has image sensor circuit board and image sensor, and it has focusing lens to have in, objective, collimating mirror, arouse the optical filter, dichroic mirror and collection optical filter, be equipped with the awl battery of lens between dichroic mirror and the collimating mirror, the awl battery of lens includes awl lens one and awl lens two, the awl end of awl lens one and awl lens two sets up focusing lens relatively and corresponds with image sensor, there is the light source outside the collimating mirror, light source, image sensor circuit board and outside data acquisition card data connection. The utility model can adapt to different tested objects to make corresponding adjustment in a very short time, expands the application range and application scene of the micro-imaging device, and can effectively reduce the mirror reflection efficiency and inhibit stray light signals by annular light illumination when the tested samples are high-reflectivity samples such as industrial samples.
Description
Technical Field
The utility model relates to a multifunctional miniature fluorescent dark field microscopic imaging device, and belongs to the technical field of microscopic imaging.
Background
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence to study the properties of organic or inorganic substances instead of, or in addition to, scattering, reflection, attenuation, or absorption. Fluorescence microscope refers to any microscope that uses fluorescence to generate an image, whether a simpler setup such as a radiofluorescence microscope or a more complex design such as a confocal microscope.
Understanding of technology by modern technological developments has recognized that miniaturized integration is a key advance in facilitating low cost production, often leading to improved performance and unexpected applications. This impact has been manifested in various areas including communications and computer and like technologies.
Optical microscopy is a largely non-integratable technique and remains a large and expensive bench-top instrument.
Most of the existing wide-field microscopes are scientific research instruments and equipment, are large in size and high in price, are generally only suitable for places such as hospitals, research institutes and colleges, and cannot be widely used. And advanced microscope operation is relatively complicated and tedious, and personnel who have not been trained by professionals cannot operate the microscope. In addition, the prior art is only suitable for experimental mice or other animals with smaller body types after fluorescent protein injection, and the tested object is single. Meanwhile, the prior art cannot realize accurate observation of any sample structure.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems in the background technology, the utility model provides a multifunctional miniature fluorescent dark field microscopic imaging device.
The utility model adopts the following technical scheme: a multifunctional micro fluorescent dark field microscopic imaging device comprises a collimating mirror, an excitation optical filter, a dichroic mirror, an objective lens, a collection optical filter, a focusing lens, an image sensor, a light source, a camera adapter, an image sensor circuit board, an outer shell and a conical lens group; the camera adapter is characterized in that a first optical channel is arranged at the upper end of the outer shell, a second optical channel is arranged at the bottom end of the outer shell, a third optical channel is arranged at the side end of the outer shell, the first optical channel and the second optical channel are coaxially communicated, the third optical channel and the second optical channel are vertically communicated, the upper end of the outer shell is connected with the camera adapter in an adjustable and limiting manner, an image sensor circuit board is arranged at the upper end of the camera adapter, an image sensor is integrated at the lower end of the image sensor circuit board, a focusing lens is arranged at a light outlet of the first optical channel, and the focusing lens is arranged corresponding to the image sensor; an objective lens is arranged in the second optical channel; a collimating lens is arranged at the outer end part of the third optical channel, a light source is arranged at the outer side of the collimating lens and connected with the outer shell, a dichroic mirror is arranged at the communication part of the third optical channel and the second optical channel and used for reflecting light of the third optical channel into the second optical channel, a collecting optical filter is arranged between the dichroic mirror and the focusing lens, an excitation optical filter and a conical lens group are arranged between the dichroic mirror and the light source, the conical lens group is arranged adjacent to the light source and comprises a first conical lens and a second conical lens, and conical ends of the first conical lens and the second conical lens are oppositely arranged; the light source is in data transmission connection with the image sensor circuit board, and the image sensor circuit board is in data transmission connection with the external data acquisition card.
Compared with the prior art, the utility model has the beneficial effects that:
1. the utility model can rapidly assemble, disassemble and focus the micro-imaging device by arranging the lens protective cover and the camera adapter, is convenient for updating and replacing various components and can adapt to corresponding adjustment of different tested objects in a very short time.
2. The microscope adaptor II is arranged, the optical connecting rod is connected through the threads, the portable microscope can be quickly built, imaging detection can be performed on living animals, imaging detection can also be performed on samples of standard glass slide samples, microfluidic chips and industrial chips, and the application range and application scene of the micro-imaging device are expanded.
3. The device comprises a light source, a collimating lens, a conical lens group, a beam splitter, a focusing lens, a transmission lens, a focusing lens, an image sensor, a cone lens group, a transmission lens, a focusing lens, a cone lens group, a beam splitter, a focusing lens and a focusing lens. When the detected sample is a high-reflectivity sample such as an industrial sample, the annular light illumination can effectively reduce the mirror reflection efficiency and inhibit a stray light signal.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view of FIG. 1;
FIG. 3 is an exploded view of FIG. 1;
FIG. 4 is a schematic view of the mounting of the objective lens;
FIG. 5 is a schematic view of the mounting of a first microscope adapter to the outer housing;
FIG. 6 is a schematic view of the mounting of the second microscope adapter to the outer housing;
FIG. 7 is a schematic view of the installation of the first microscope adapter, the second microscope adapter and the outer housing;
FIG. 8 is a use state reference diagram of the present invention;
FIG. 9 is an image of an onion section specimen taken in accordance with the present invention;
FIG. 10 is an image of a kidney section specimen in accordance with the present invention;
FIG. 11 is an image of a wafer structure according to the present invention;
FIG. 12 is an image of the present invention taken with a standard resolution plate.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.
A multifunctional micro fluorescent dark field microscopic imaging device comprises a collimating mirror 1, an excitation optical filter 2, a dichroic mirror 3, an objective lens 4, a collection optical filter 5, a focusing lens 6, an image sensor 7, a light source 8, a camera adapter 14, an image sensor circuit board 17, an outer shell 22 and a cone lens group; the outer shell 22 is a carrier of all optical elements and circuit elements of the present invention, and is made of black resin, or any hard metal or nonmetal material, the upper end of the outer shell 22 is provided with a first optical channel, the bottom end of the outer shell 22 is provided with a second optical channel, and the side end of the outer shell 22 is provided with a third optical channel, the first optical channel and the second optical channel are coaxially communicated to form a reflective optical path; the third light channel is vertically communicated with the second light channel to form an illumination light path; the illumination light path is as follows according to the light propagation direction in sequence: the light source 8-the collimating lens 1-the dichroic mirror 3-the objective lens 4, the reflected light path shines the propagation direction of light and is in proper order: the objective lens 4-the dichroic mirror 3-the focusing lens 6-the image sensor 7; the upper end of the outer shell 22 is connected with the camera adaptor 14 in an adjustable limit manner (the camera adaptor 14 is responsible for connecting the image sensor circuit board 17 with the outer shell 22. in the utility model, the lower half part of the camera adaptor 14 is a male sliding groove which is in sliding clearance fit with a female sliding groove of the upper half part of the outer shell 22, the adjustable limit fixing connection is carried out through a first jackscrew 18 and a second jackscrew 19 which are arranged on the lateral side of the male sliding groove of the camera adaptor 14, the outer shell 22 and the side wall of the camera adaptor 14 are correspondingly provided with two threaded holes M1.4, but not limited to the range, the first jackscrew 18 and the second jackscrew 19 are arranged in the corresponding threaded holes), the upper end of the camera adaptor 14 is provided with the image sensor circuit board 17 (the upper half part of the camera adaptor 14 is fixedly connected with the image sensor circuit board 17 through a fourth fastening screw 15, a fifth fastening screw 16 and a sixth fastening screw 23, keeping the volume to a minimum. Since the objective lens 4 is fixed, the camera adaptor 14 needs to move up and down to find the focusing plane, and besides the sliding adjustment mode, the adjustment mode can also be in the form of screw rotation, sawtooth groove and the like. The camera adaptor 14 is made of resin, and can be made of any hard metal or nonmetal material), the lower end of the image sensor circuit board 17 is integrated with the image sensor 7, and the image sensor can be any type of photosensitive element such as a CMOS, a CCD, a photomultiplier tube, an avalanche diode and the like;
the image sensor circuit board 17 is used for controlling parameters such as exposure gain of the image sensor 7, a focusing lens 6 is arranged at a light outlet of the first optical channel, and the focusing lens 6 is arranged corresponding to the image sensor 7; an objective lens 4 is arranged in the second optical channel; a collimating mirror 1 is arranged at the outer end part of the third optical channel, a light source 8 is arranged on the outer side of the collimating mirror 1 through a third fastening screw 20, the light source 8 is connected with an outer shell 22, a dichroic mirror 3 is arranged at the communication part of the third optical channel and the second optical channel, and the dichroic mirror 3 is used for reflecting light of the third optical channel into the second optical channel; a collecting filter 5 is arranged between the dichroic mirror 3 and the focusing lens 6, an excitation filter 2 and a cone lens group are arranged between the dichroic mirror 3 and the light source 8, the cone lens group is arranged adjacent to the light source, the cone lens group comprises a first cone lens 41 and a second cone lens 42, and the cone ends of the first cone lens 41 and the second cone lens 42 are oppositely arranged;
the duty ratio of the annular light beam can be adjusted by changing the conical surface angle of the conical lens, and the annular light illumination can effectively reduce the mirror reflection efficiency and inhibit noise. The generation method of the annular light is not limited to the cone lens group, and can be a specially designed mask or metamaterial lens.
The objective lens 4 is an infinite objective lens, a long-working-distance gradient refractive index lens is selected in the utility model, more specifically, a gradient refractive index lens with 0.23 pitch is selected, illumination light is modulated and reflected by a dichroic mirror and then vertically enters a first end face of the objective lens 4, and then is transmitted through a second end face of the objective lens 4 to be focused on a sample to be detected to generate excitation fluorescence. The reflected light passes through the objective lens 4, the dichroic mirror 3 and the focusing lens 6 and then is converged to the image sensor 7 for imaging.
The objective lens 4 may be a plano-convex lens, a cemented lens, a spherical lens, or the like, which has a larger aberration than a gradient index lens, or may be a metamaterial lens attached to a glass substrate or other transparent medium. The diameter d2 of the objective 4 is dimensioned such that: d2 is more than 25.4mm and more than or equal to 0.1mm, and the focal length phi satisfies the following conditions: the focal length selection can influence the imaging visual field, the resolution, the magnification factor and other indexes when the diameter is more than 30mm and is more than or equal to 0mm, and the working distance meets 10-40 mm.
The light source 8 is an LED light source or an optical fiber, the LED light source is a white light source, the wavelength is 450-490nm, the light source can be a non-visible near-infrared light source, the light source can also be a narrow-band light source with any bandwidth, and the light source selection can influence the imaging resolution according to the airy disc formula of 0.61 lambda/NA; the light source may be a laser, a halogen lamp, or other types of light sources. The LED patches are packaged on the light source 8, the packages can be 0402, 0603 and the like, the thickness is 0.8mm but not limited to the thickness, and the LED patches are manufactured into a heat dissipation hole process and have any color.
The collimating mirror 1 can be any optical element or non-optical element that collimates light, such as a hemispherical lens, a spherical lens, a drum lens, a gradient index lens, a cylindrical lens, etc., with the dimensions of the lens diameter d1 being such that: the thickness of 25.4mm is more than d1 and is more than or equal to 0.1mm, the thickness of 25.4mm is the common size of the conventional optical element, and the size of more than or equal to the common size is not included in the range of miniaturization or miniaturization equipment. The non-optical element can be a metamaterial lens, and the metamaterial lens can be attached to a smaller and thinner glass substrate or other transparent media, so that the whole size is lighter and smaller, and special light wave transmission requirements can be realized.
The focusing lens 6 is an achromatic cemented lens, in the utility model, the light source wavelength is 460-490nm, and the use of the achromatic lens can improve the imaging quality. The focusing lens 6 may be a plano-convex lens, a spherical lens, or the like. The diameter d3 of the focusing lens 6 has dimensions such that: 25.4mm > d3 is more than or equal to 1mm, and the common focal length is 5mm, 7mm, 10mm, 15mm, 20mm and the like, but is not limited in the range.
The image sensor 7 is a color or black and white image sensor. The pixel number is 752 multiplied by 480(360960 pixels), the pixel size is 6 mu m, the maximum pixel sampling frame rate can reach 60fps, the shutter efficiency is more than 99 percent, the double-line serial interface, the Automatic Exposure Control (AEC) and the Automatic Gain Control (AGC), and the AEC/AGC with variable area and variable weight can meet the imaging requirement under most conditions.
The dichroic mirror 3 is a long-wavelength pass dichroic mirror of 500nm, and similarly, the excessive wavelength may be an arbitrary frequency band of visible light. The dichroic mirror can be replaced by a light splitting film, and the weight and the volume of the miniature microscopic imaging device are further reduced.
The light source 8 is connected with the image sensor circuit board 17 through a single-core lead 40 in a data transmission mode, and the on-off and the brightness of the LED light source circuit board 8 can be controlled. The image sensor circuit board 17 is connected with an external data acquisition card through a flexible shielding wire 39 in a data transmission manner to supply power and transmit data, and the torque of the miniature wide-field microscopic imaging device can be increased by using the flexible wire to prevent the miniature wide-field microscopic imaging device from being damaged;
the vertical plane of the detection light path is an X-Y plane, the parallel is a Z axis, the size in the XY direction is about 1cm multiplied by 1cm, the size in the Z direction is about 2cm, and the volume of the miniature wide-field microscopic imaging device is about 2cm3The weight was about 2 g. Due to different design schemes, the size in XYZ direction can be expanded to 0.1cm to 20cm, and the volume can be 0.1cm3To 100cm3The weight can be 0.1g to 1000g, the sizes are not limited to the range, and the overlarge value is not a micro microscopic imaging device in a macroscopic sense but a traditional large-volume table-type microscope.
An objective lens adapter 11 is arranged between the objective lens 4 and the second optical channel, the objective lens adapter 11 is responsible for connecting the objective lens 4 and the outer shell 22, the diameter of the objective lens 4 can be designed to be consistent with the diameter of the second optical channel, but the wider application of the objective lens is limited, so the diameter of the second optical channel is slightly larger than the diameter of the objective lens 4, and the objective lens adapter 11 needs to be designed to fixedly connect the objective lens 4 and the outer shell 22. The objective lens adapter 11 is tightly fitted on the outside of the objective lens 4, and the objective lens adapter 11 is tightly inserted into the second optical channel. The objective adapter 11 is made of resin and manufactured by using a 3D printing technology, and may be made of metal or nonmetal such as copper, iron, and aluminum alloy.
The outer wall of the objective lens adapter piece 11 is provided with a limiting protrusion along the circumferential direction, the limiting protrusion is arranged on the outer side of the outer shell 22, the limiting protrusion limits the position of the objective lens adapter piece 11 penetrating into the second optical channel, and collision between the objective lens 4 and the dichroic mirror 3 is prevented.
The outer side of the outer shell 22 is detachably and fixedly connected with the lens protection cover 25 through a first fastening screw 13 and a second fastening screw 21 (the size of the fastening screw is M1 multiplied by 3, but not limited to the range), a magnet, a mortise and tenon structure, a groove structure, glue fixation and the like.
The lens protective cover 25 can protect the internal optical elements from being polluted, and can also enable the light path transmission route to be in a completely closed state, so that the influence of stray light is reduced, and the imaging quality is improved. The lens protection cover 25 may be made of resin, and may be made of any hard metal or nonmetal.
The lower end of the outer shell 22 is provided with a first microscope adapter 12 and/or a second microscope adapter 31, and when the lower end of the outer shell 22 is provided with the first microscope adapter 12 and the second microscope adapter 31, the second microscope adapter 31 is arranged at the lower end of the first microscope adapter 12.
The lower end of the outer shell 22 is detachably and fixedly connected with the microscope adaptor I12.
The microscope adaptor 12 and the outer shell 22 are fixed through the magnetic suction mode, so that the microscope adaptor and the outer shell are convenient to install and disassemble, fixing modes including but not limited to light ultraviolet glue and the like can be achieved, and structures such as threads, clamping grooves and mortise and tenon joints can be used for fixing. All the magnets of the present invention have a diameter of 1mm and a thickness of 1mm, but are not limited thereto.
The jackscrew four 26 fixes the objective adapter 11 and the outer housing 22. The back surface of the microscope adapter piece I12 is provided with a U-shaped groove to avoid the jackscrew II 26, so that the microscope adapter piece I12 and the objective lens adapter piece 11 do not conflict when being used simultaneously.
The microscope adaptor two 31 is arranged at one end of the optical extension rod one 32, the other end of the optical extension rod one 32 is arranged on the extension rod adaptor 33, the extension rod adaptor 33 is arranged on the XYZ displacement table 35, and the optical extension rod two 34 is arranged on the extension rod adaptor 33.
The present invention can constitute a bench microscope with an extremely simple structure as shown in fig. 6, and functions as the conventional bench microscope. The optical extension rod one 32, the extension rod adaptor 33, the optical extension rod two 34 and the XYZ displacement table 35 used in fig. 6 are all the most commonly used optical-mechanical devices and displacement platforms in a laboratory, fig. 6 only shows an assembly manner, and in practical applications, there are various alternatives, such as the length of the optical extension rod, the connection manner, and the like, and the displacement table can be a single-axis displacement table, a double-axis displacement table, a three-axis displacement table, a four-axis displacement table, a five-axis displacement table, a six-axis displacement table, and the like.
The microscope adapter piece II 31 has two types which can be selected, the internal threads corresponding to the side surface of the microscope adapter piece II 31 are M3 and M4, and the two types are common adapter types of laboratory optical connecting rods. The bottom end of the microscope adaptor second 31 can be connected with the microscope adaptor first 12 through three magnets at corresponding positions, and can also be connected with the outer shell 22, and using methods are different according to different requirements. The material of the second microscope adaptor 31 is resin, and may be any hard metal or nonmetal material.
A still imaging method of a multifunctional miniature fluorescent dark-field microscopic imaging device, comprising the following steps:
s1: connecting the objective 4 to the outer housing 22 via the objective adapter 11;
s2: the utility model is held by hand, the focal plane of the objective lens 4 is aligned with the sample to be measured;
s3: turning on the light source 8;
s4: light rays emitted by the light source 8 form parallel light beams after passing through the collimating lens 1 for transmission;
s5: after the parallel light beams reach the dichroic mirror 3, the parallel light beams are reflected to change the transmission direction to the objective lens 4;
s6: the objective lens 4 converges the parallel light beams to the surface of the measured sample;
s7: the measured sample generates reflected light;
s8: the reflected light is modulated by the objective lens 4 to form parallel light, and the parallel light reaches the dichroic mirror 3 and then is transmitted to the focusing objective lens 6;
s9: the focusing objective 6 focuses the parallel light to the surface of the image sensor 7 for imaging.
A living animal imaging method of a multifunctional miniature fluorescent dark-field microscopic imaging device is suitable for imaging free-moving living animals, such as in-situ imaging of rodent brains or other in-vivo tissues. The method comprises the following steps:
s1: connecting the objective 4 to the outer housing 22 via the objective adapter 11;
s2: mounting the microscope adaptor one 12 at the lower end of the outer shell 22;
s3: aligning the focal plane of the objective lens 4 to the sample to be measured;
s4: turning on the light source 8;
s5: light rays emitted by the light source 8 form parallel light beams after passing through the collimating lens 1 for transmission;
s6: after the parallel light beams reach the dichroic mirror 3, the parallel light beams are reflected to change the transmission direction to the objective lens 4;
s7: the objective lens 4 converges the parallel light beams to the surface of the measured sample;
s8: the measured sample generates reflected light;
s9: the reflected light is modulated by the objective lens 4 to form parallel light, and the parallel light reaches the dichroic mirror 3 and then is transmitted to the focusing objective lens 6;
s10: the focusing objective 6 focuses the parallel light to the surface of the image sensor 7 for imaging.
A laboratory sample imaging method for a multifunctional miniature fluorescent dark-field microscopic imaging device, the method comprising the steps of:
s1: connecting the objective 4 to the outer housing 22 via the objective adapter 11;
s2: connecting a microscope adaptor II 31, an optical connecting rod I32, a connecting rod adaptor 33, an optical connecting rod II 34 and an XYZ displacement table 35 at one time;
s3: installing the microscope adapter piece I12 at the lower end of the outer shell 22, and then installing the microscope adapter piece II 31 at the lower end of the microscope adapter piece I12;
or directly installing the second microscope adapter 31 at the lower end of the outer shell 22;
s4: aligning the focal plane of the objective lens 4 to the sample to be measured;
s5: turning on the light source 8;
s6: light rays emitted by the light source 8 form parallel light beams after passing through the collimating lens 1 for transmission;
s7: after the parallel light beams reach the dichroic mirror 3, the parallel light beams are reflected to change the transmission direction to the objective lens 4;
s8: the objective lens 4 converges the parallel light beams to the surface of the measured sample;
s9: the measured sample generates reflected light;
s10: the reflected light is modulated by the objective lens 4 to form parallel light, and the parallel light reaches the dichroic mirror 3 and then is transmitted to the focusing objective lens 6;
s11: the focusing objective 6 focuses the parallel light to the surface of the image sensor 7 for imaging.
The object provided by the utility model is really manufactured according to the utility model, can be used, and does not have false enlarged protection range. The eighth group of elements in the fifth row of the standard resolution plate can be resolved in fig. 12, the resolution is about 1 μm, the diameter of the field of view is about 2mm, but the resolution and the field of view are not limited to the sub-resolution and the field of view, and the resolution and the field of view of the optical system are mutually restricted and can be higher or lower, fig. 9 and 10 show the imaging effect of the biological sample, fig. 11 shows the imaging effect of the industrial sample, and the structures of the samples in fig. 9, 10 and 11 are all in the micron level.
The utility model customizes or self-designs suitable optical elements such as an objective lens, a tube lens and the like, a CMOS sensor with a micro size and a data acquisition card, and manufactures a micro fluorescence microscopic imaging device. On the premise that core indexes such as a view field and resolution are not weaker than that of a traditional table microscope, the equipment greatly reduces the volume of the equipment, and can show the advantages of microscopic imaging in many fields which cannot be realized originally, such as in-vivo imaging of freely moving organisms. In addition, the multifunctional multiplexing of a handheld fluorescence microscope, a suspended fluorescence microscope and a desk-top fluorescence microscope can be realized through the adapter tool. Allowing mass production and maintaining high stability comparable to conventional microscopes. The utility model is based on micro-optics and semi-conductor, both of which are easy to manufacture in large quantities and at low cost, and compared with high resolution fiber optic microscopes, our device has significant advantages in terms of optical sensitivity, field of view, resolution, mechanical flexibility, cost and portability.
The top end of the outer shell is provided with a receiving light path, the side of the outer shell is provided with a transmitting light path, a light source capable of controlling light intensity is arranged outside the transmitting light path, and an image sensor 7 for receiving image information by a miniaturized CMOS is arranged above the receiving light path.
The incident parallel optical signals are parallelly emitted through the collimating lens, an annular light source is formed after the incident parallel optical signals pass through the conical lens group, the annular light source is adjusted and reflected to the gradient refractive index lens through the dichroic mirror, the emitted optical signals are focused into a biological or industrial sample to be detected, the annular light illumination can effectively inhibit stray light signals, and therefore efficient detection of a sample structure is achieved. The returned fluorescence is converged into a miniaturized CMOS for imaging through a dichroic mirror and a tube mirror.
The utility model can realize a handheld micro microscope by matching with the objective lens adapter, and the equipment can realize in-vivo imaging of freely moving organisms by matching with the microscope adapter. The function of a conventional fluorescence microscope can be realized by matching the objective lens adapter piece and the microscope adapter piece II.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present specification describes embodiments, not every embodiment includes only a single embodiment, and such description is for clarity purposes only, and it is to be understood that all embodiments may be combined as appropriate by one of ordinary skill in the art to form other embodiments as will be apparent to those of skill in the art from the description herein.
Claims (6)
1. A multifunctional miniature fluorescent dark field microscopic imaging device is characterized in that: the device comprises a collimating lens (1), an excitation optical filter (2), a dichroic mirror (3), an objective lens (4), a collecting optical filter (5), a focusing lens (6), an image sensor (7), a light source (8), a camera adapter (14), an image sensor circuit board (17), an outer shell (22) and a cone lens group; the upper end of the outer shell (22) is provided with a first optical channel, the bottom end of the outer shell (22) is provided with a second optical channel, a third optical channel is arranged at the side end of the outer shell (22), the first optical channel and the second optical channel are coaxially communicated, the third optical channel and the second optical channel are vertically communicated, the upper end of the outer shell (22) is connected with the camera adaptor (14) in an adjustable and limiting manner, the upper end of the camera adaptor (14) is provided with an image sensor circuit board (17), the lower end of the image sensor circuit board (17) is integrated with an image sensor (7), a light outlet of the first optical channel is provided with a focusing lens (6), and the focusing lens (6) and the image sensor (7) are correspondingly arranged; an objective lens (4) is arranged in the second optical channel; a collimating mirror (1) is arranged at the outer end part of the third optical channel, a light source (8) is arranged on the outer side of the collimating mirror (1), the light source (8) is connected with an outer shell (22), a dichroic mirror (3) is arranged at the communication part of the third optical channel and the second optical channel, and the dichroic mirror (3) is used for reflecting light of the third optical channel into the second optical channel; a collecting filter (5) is arranged between the dichroic mirror (3) and the focusing lens (6), an excitation filter (2) and a cone lens group are arranged between the dichroic mirror (3) and the light source (8), the cone lens group is arranged adjacent to the light source (8), the cone lens group comprises a first cone lens (41) and a second cone lens (42), and the cone ends of the first cone lens (41) and the second cone lens (42) are oppositely arranged; the light source (8) is in data transmission connection with the image sensor circuit board (17), and the image sensor circuit board (17) is in data transmission connection with an external data acquisition card.
2. The multifunctional micro fluorescent dark-field microscopic imaging device according to claim 1, characterized in that: an objective lens adapter (11) is arranged between the objective lens (4) and the second optical channel, the objective lens adapter (11) is tightly sleeved on the outer side of the objective lens (4), and the objective lens adapter (11) is tightly inserted into the second optical channel.
3. The multifunctional micro fluorescent dark-field microscopic imaging device according to claim 2, characterized in that: the outer wall of objective lens adaptor (11) is equipped with spacing arch along its circumference, spacing arch sets up the outside in shell (22).
4. The multifunctional micro fluorescent dark-field microscopic imaging apparatus according to claim 1 or 3, wherein: the outer side of the outer shell (22) is detachably and fixedly connected with the lens protection cover (25).
5. The multifunctional micro fluorescent dark-field microscopic imaging device according to claim 4, characterized in that: the microscope adapter is characterized in that a first microscope adapter (12) and/or a second microscope adapter (31) are/is arranged at the lower end of the outer shell (22), and when the first microscope adapter (12) and the second microscope adapter (31) are arranged at the lower end of the outer shell (22), the second microscope adapter (31) is arranged at the lower end of the first microscope adapter (12).
6. The multifunctional micro fluorescent dark-field microscopic imaging device according to claim 5, characterized in that: the microscope adaptor II (31) is arranged at one end of the optical extension rod I (32), the other end of the optical extension rod I (32) is arranged on the extension rod adaptor (33), the extension rod adaptor (33) is arranged on the XYZ displacement table (35), and the optical extension rod II (34) is arranged on the extension rod adaptor (33).
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| CN202122807307.0U CN216525449U (en) | 2021-11-16 | 2021-11-16 | Multifunctional miniature fluorescent dark field microscopic imaging device |
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| CN202122807307.0U CN216525449U (en) | 2021-11-16 | 2021-11-16 | Multifunctional miniature fluorescent dark field microscopic imaging device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115166960A (en) * | 2022-07-19 | 2022-10-11 | 华南师范大学 | LED excitation light source for quantitative FRET microscopic imaging and dimming method |
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2021
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115166960A (en) * | 2022-07-19 | 2022-10-11 | 华南师范大学 | LED excitation light source for quantitative FRET microscopic imaging and dimming method |
| CN115166960B (en) * | 2022-07-19 | 2023-08-04 | 华南师范大学 | LED excitation light source for quantitative FRET microscopic imaging and dimming method |
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