CN112680382A - Bacillus belgii and application thereof - Google Patents
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Abstract
The invention discloses a Bacillus velezensis strain and application thereof, wherein the strain is preserved in China general microbiological culture Collection center in 12 months and 07 days in 2020, the preservation number is CGMCC No.21318, and the preservation name is Bacillus velezensis TYGF10-2F 9. The biological membrane can be applied to the field of agricultural biological control, can form a biological membrane to stably colonize at plant rhizosphere, can generate strong and broad-spectrum inhibition effect on 12 plant pathogenic fungi and oomycetes, and can generate IAA, siderophore and other substances to promote plant growth. The Bacillus beilesiensis fermentation liquor has the control effect on rape sclerotinia rot potted plants as high as 80.99 percent; the Bacillus belgii suspension and the fermentation liquor have the prevention effect on cucumber wilt potted plants as high as 66.67 percent, have a certain growth promotion effect on cucumbers, are biocontrol strains with excellent performance, and have good development and application prospects.
Description
Technical Field
The invention belongs to the technical field of agricultural biological control, and particularly relates to a Bacillus subtilis and application thereof.
Background
Plant diseases pose serious threats to world food production and safety. For a long time, the use of chemical pesticides and fertilizers makes the problems of environmental pollution, bacterial strain drug resistance, soil micro-ecological imbalance and the like increasingly worse. There is a need to develop microbial products that can replace chemical pesticides and fertilizers. Although a large number of microbial products exist, due to the phenomena of low colonization ability in the field, short duration and the like, the mining of broad-spectrum and efficient microbes from plant-related microbial communities such as rhizosphere and root endophytic microorganisms becomes a research hotspot of scholars at home and abroad.
The bacillus is a kind of spore-producing gram-positive bacteria, and most of the bacillus has the advantages of wide antibacterial spectrum, rapid growth, easy separation and culture, strong stress resistance, high biological safety and the like, so that the bacillus is widely researched as a probiotic in the aspects of agriculture, food, industry, medicine, metallurgy, forestry, environmental protection, military and the like. Bacillus velezensis as a newly discovered species in Bacillus is widely distributed in nature, can be separated from marine and river sediments, soil, plant rhizosphere and plant tissues, is harmless to human and livestock, has no pollution to environment, and can generate secondary metabolites with antagonistic action on various pathogenic microorganisms. In recent years, research on bacillus belgii has been mainly focused in the fields of feed, medicine, textile, aquatic products, sewage treatment, plant protection, and the like. Bacillus belgii has attracted attention as a novel biocontrol microbial agent in the fields of plant disease control, plant growth promotion, and the like.
Disclosure of Invention
The invention aims to provide a Bacillus velezensis TYGF10-2F9 strain and application thereof. The Bacillus velezensis TYGF10-2F9 provided by the invention has been preserved in China general microbiological culture Collection center (CGMCC for short, address: No. 3 of No. 1 Hospital of West Lu of North West of the Korean area in Beijing city) in 12 months and 07 days in 2020, and CGMCC No.21318
The preservation number of the Bacillus belgii strain, Bacillus velezensis, is CGMCC No. 21318.
The bacillus beilesensis is used for preparing plant bacteriostatic agents or medicines for preventing and treating plant diseases caused by phytopathogens.
Optionally, the plant pathogenic bacteria are selected from sclerotium napellus (sclerotium sclerotiorum), Rhizoctonia solani (Rhizoctonia solani), gibberella zeae (Fusarium graminearum), verticillium pomorum (botryococcus berengiana), alternaria nicotianae (alternaria alternata), Phytophthora capsici (Phytophthora capsici), Fusarium oxysporum f.sp.niveum, Fusarium oxysporum (cucumber oxysporum sp.sp.wen), strawberry Fusarium oxysporum f.sp.fragrans, Botrytis cinerea (Botrytis cinerea), Botrytis cinerea (Valsa maylia yama mayana) and Fusarium oxysporum (wheat germ).
The Bacillus belgii is applied to preparation of plant colonizers, plant biocontrol agents, bio-organic fertilizers or plant growth promoters.
The preparation is characterized by being bacterial suspension, fermentation liquor, fermentation product or secondary metabolite of the bacillus beilesensis.
The preparation is used for preparing plant bacteriostat or medicines for preventing and treating plant diseases caused by plant pathogenic bacteria.
Optionally, the plant pathogenic bacteria are selected from sclerotium napellus (sclerotium sclerotiorum), Rhizoctonia solani (Rhizoctonia solani), gibberella zeae (Fusarium graminearum), verticillium pomorum (botryococcus berengiana), alternaria nicotianae (alternaria alternata), Phytophthora capsici (Phytophthora capsici), Fusarium oxysporum f.sp.niveum, Fusarium oxysporum (cucumber oxysporum sp.sp.wen), strawberry Fusarium oxysporum f.sp.fragrans, Botrytis cinerea (Botrytis cinerea), Botrytis cinerea (Valsa maylia yama mayana) and Fusarium oxysporum (wheat germ).
The preparation method of IAA adopts the Bacillus beiLeisi to prepare IAA.
A preparation method of a siderophore adopts the Bacillus belgii to prepare the siderophore.
The Bacillus belgii is applied to the field of agricultural biological control, can form a biological film to stably colonize at plant rhizosphere, can generate strong and broad-spectrum inhibition effect on 12 plant pathogenic fungi and oomycetes, and can generate IAA, siderophore and other substances to promote plant growth. The Bacillus beilesiensis fermentation liquor has the control effect on rape sclerotinia rot potted plants as high as 80.99 percent; the Bacillus belgii suspension and the fermentation liquor have the prevention effect on cucumber wilt potted plants as high as 66.67 percent, have a certain growth promotion effect on cucumbers, are biocontrol strains with excellent performance, and have good development and application prospects.
Drawings
The accompanying drawings, which are included to provide a further understanding of the disclosure and are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description serve to explain the disclosure without limiting the disclosure. In the drawings:
FIG. 1 is a developing tree of Bacillus velezensis TYGF10-2F9 provided in example 1 of the present invention;
FIG. 2 shows the bacteriostatic activity of Bacillus velezensis TYGF10-2F9 on 12 strains of pathogenic fungi, which is provided in example 2 of the present invention; a and G represent rice sheath blight; b and H represent gibberella tritici; c and I represent sclerotium of colza; d and J represent apple ring lines; e, K represents tobacco gibberellin; f and L represent phytophthora capsici; m and S represent watermelon wilt; n, T represents cucumber wilt; o, U represents strawberry withering; p and V represent Botrytis cinerea; o, W represents apple rot; r and X represent the wheat full-etch;
FIG. 3 is a standard curve of IAA production by Bacillus velezensis TYGF10-2F9 according to example 3 of the present invention;
FIG. 4 shows the formation of biofilm in various media by Bacillus velezensis TYGF10-2F9 according to example 4 of the present invention; a, B, C, D represent the biofilms of the strain TYGF10-2F9 in the media LBG, LBM, LBGM and LB, respectively;
FIG. 5 shows the potted control effect of Bacillus velezensis TYGF10-2F9 on sclerotinia rot of rape as provided in example 5 of the present invention;
FIG. 6 shows the control effect of Bacillus velezensis TYGF10-2F9 on sclerotium rolfsii according to example 5 of the present invention;
FIG. 7 shows the potting control effect of Bacillus velezensis TYGF10-2F9 on cucumber fusarium wilt, provided in example 6 of the present invention.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The plant colonization agent, the plant biocontrol agent, the bio-organic fertilizer, the plant growth promoter and the like are all common formulations such as fertilizers or nutrient solutions and the like commonly used in agriculture. The Bacillus belgii is applied to the field of agricultural biological control, can form a biological film to stably colonize at plant rhizosphere, can generate strong and broad-spectrum inhibition effect on 12 plant pathogenic fungi and oomycetes, and can generate IAA, siderophore and other substances to promote plant growth. The Bacillus beilesiensis fermentation liquor has the control effect on rape sclerotinia rot potted plants as high as 80.99 percent; the Bacillus belgii suspension and the fermentation liquor have the prevention effect on cucumber wilt potted plants as high as 66.67 percent, have a certain growth promotion effect on cucumbers, are biocontrol strains with excellent performance, and have good development and application prospects.
Example 1 isolation and purification of the Strain TYGF10-2F9 and molecular biological characterization
Weighing 10g of wheat rhizosphere soil, adding 200mL of 0.01M sterile PBS buffer solution, shaking for 30min at room temperature under the condition of 180r/min to obtain rhizosphere soil suspension, and performing gradient dilution to 10 by using sterile water-1、10-2And 10-3And uniformly coating the mixture on a TYG (tyrosine Yeast extract Glucose Medium, TYG) culture Medium by using a coating rod, wherein the composition of the TYG culture Medium is as follows: tryptone 1g, yeast extract 1g, glucose 0.5g, KCl 6.34g, NaCl 1.2g, MgSO4·7H2O 0.25g、K2HPO4 0.13g、CaCl2·2H2O 0.22g、K2SO4 0.17g、Na2SO4 2.4g、NaHCO3 0.5g、Na2CO30.09g, 0.07g of FeEDTA, 15g of agar, 1000mL of distilled water, and 7.2-7.4 of PH; culturing at 28 deg.C for 2d, and selecting single colony for purification culture.
DNA extraction was performed according to the procedure of the column type bacterial DNA extraction kit of Shanghai Biotechnology engineering (Shanghai) Ltd. Selecting a bacterial universal primer pair: f275 '-AGA GTT TGA TCC TGG CTC AGG-3', P15415 '-AAG GAG GTG GTG ATC CAG CCG CA-3'. The reaction conditions are as follows: denaturation at 94 ℃ for 45s, annealing at 50 ℃ for 45s, extension at 72 ℃ for 75s, and 50. mu.L of the reaction system for 30 cycles. And detecting the PCR product by agarose gel electrophoresis, and obtaining a sequence result by cloning and sequencing. The obtained 16S rDNA complete sequence is compared with the 16S rDNA sequence obtained from databases such as Genbank and the like, and the phylogenetic tree is constructed through a Mega 6.0 software package.
The strain TYGF10-2F9 was identified by molecular biology methods. The determination result of the 16S rDNA base sequence of the strain TYGF10-2F9 is shown in the sequence table, and 1444 bases exist in the 16S rDNA of the co-determination strain TYGF10-2F 9.
The 16S rDNA full sequence obtained by amplifying the fragment size from the genome DNA of the strain is compared with the 16S rDNA sequence obtained from the Genbank database to obtain the accession number: TYGF10-2F9(MW 314755). Construction of phylogenetic trees: 10 model strains are selected, phylogenetic analysis is carried out by adopting a Neighbor-Joining method in MEGA 6 software, and a constructed phylogenetic tree is shown in figure 1.
Example 2 bacterial Strain TYGF10-2F9 bacteriostatic assay
The inhibition of the fungal action of sclerotium rolfsii (Sclerotinia sclerotiorum), rice sheath blight (Rhizoctonia solani), gibberella zeae (Fusarium graminearum), apple ring rot (botryosphaera berangeiana), tobacco red star (alternaria alternata), Phytophthora capsici (Phytophthora capsici), watermelon wilt (Fusarium oxysporum f.sp.niveum), cucumber wilt (Fusarium oxysporum.sp.niveum), strawberry wilt (Fusarium oxysporum f.sp.agararia), tomato gray mold (Botrytis cinerea), apple rot (Valsa mali Miyabe et yama) and wheat holo-mangostis (wheat germ) plant pathogen (germ) 12. co-occurrence by the plate confronting method. Firstly, taking the center of a culture dish as a central point, cross-scribing at the bottom of the culture dish, and inoculating a strain which is activated on an LB culture medium at a position 2.5cm away from the central point; then, the activated plant pathogenic fungi are punched by a 5mm puncher and inoculated in the center of a PDA culture medium flat plate, the culture dish is sealed, and the culture is carried out at the temperature of 28 ℃ until the radius of the pathogenic bacteria and the width of a formed transparent ring are counted when the contrast of the pathogenic bacteria grows to two thirds of the culture dish.
The bacterial strain TYGF10-2F9 bacteriostasis spectrum is evaluated by adopting a plate confronting method and taking 12 pathogenic fungi as targets. The results show that the strain TYGF10-2F9 has broad-spectrum inhibitory activity against 12 pathogenic fungi (FIG. 2). Wherein the strain TYGF10-2F9 has the highest inhibitory activity on apple rot pathogenic bacteria, and the inhibition rate is 94.06%; the inhibition activity to sclerotinia rot of colza pathogen, botrytis cinerea and wheat take all pathogenic bacteria is obvious, and the inhibition rates are respectively 88.18%, 85.24% and 82.03%; the inhibition rate of pathogenic bacteria of rice sheath blight, apple ring lines, tobacco red star, phytophthora capsici, watermelon wilt, cucumber wilt and strawberry wilt is between 60.00 and 70.00 percent; the inhibition activity on wheat scab zymogen is minimum, and the inhibition rate is 58.31% (table 1 and figure 2).
TABLE 1 inhibitory Effect of TYGF10-2F9 on 12 pathogenic fungi
Example 3 determination of growth promotion indicators for Strain TYGF10-2F9
Firstly, streaking the strain preserved at-80 ℃ on an LB culture medium, and culturing for 24h and activating in a thermostat at 28 ℃. Then, the activated strain was inoculated with a needle into a 250mL Erlenmeyer flask containing 100mL of LB liquid medium, while 1mL of L-tryptophan was added to the Erlenmeyer flask to a final concentration of 100. mu.g/mL, and cultured at 28 ℃ and 180r/min for 5 days, with 2 biological repetitions per strain. 8mL of strain fermentation liquor is taken, centrifuged at 8000r/min and 4 ℃, and the supernatant is sucked for quantitative determination of the IAA content of the strain. For the quantitative determination of IAA content, 60. mu.L of the supernatant was aspirated into a 2mL centrifuge tube, and then 120. mu.L of Salkowski reagent was added to develop color in the dark, and the absorbance of each treatment was measured at 530nm with an ultraviolet spectrophotometer. Meanwhile, a standard curve of IAA content and absorbance value was prepared using IAA solutions (50, 10, 5, 2.5, 1.25, 0.625 and 0. mu.g/mL) diluted in a gradient, and the ability of the strain to produce IAA was evaluated based on the standard curve.
The activated strain is placed in an inorganic phosphorus medium ((NH) by adopting a point inoculation method4)2SO4 0.5g，NaCl 0.3g，KCl 0.3g，MgSO4·7H2O 0.3g，Ca3(PO4)2 25g，FeSO4·7H2O 0.03g，MnSO4·H20.03g of O, 10g of glucose and 7.4-7.6 of PH), culturing for 5 days at the constant temperature of 28 ℃, and evaluating the inorganic phosphorus decomposing capacity of the strain according to whether a transparent ring is generated around the strain and the size of the transparent ring.
Inoculating the activated strain to DF medium (glucose 2.0g, gluconic acid 2.0g, citric acid 2.0g, and trace element solution (CaCl) with 3mM ACC as only nitrogen source by dot inoculation method2 200mg、FeSO4·7H2O 200mg、H3BO315mg、ZnSO4·7H2O 20mg、Na2MoO4 10mg、KI 10mg、NaBr 10mg、MnCl2 10mg、COCl2 5mg、CuCl25mg、AlCl3 2mg、NiSO42mg, 1,000mL) of deionized water 10mL, 990mL) of deionized water at 0.2% (w/v) (NH)4)2SO4DF culture as the sole nitrogen source is used as a positive control, and DF culture medium is used as a negative control. And (3) culturing the inoculated plate at the constant temperature of 28 ℃ for 5d, and comprehensively evaluating the ACC production capacity of the strains according to the growth conditions of the strains on the three culture media.
Inoculating the activated strain on a chromium azure culture medium (CAS) by a dot inoculation method, culturing the inoculated plate at 28 ℃ for 7 days, and evaluating the capability of the strain to produce the siderophore according to the appearance and the size of yellow halos around colonies.
And (3) measuring growth promoting indexes such as IAA production capability, phosphate dissolving capability, ACC production capability and siderophore production capability of the separated strain TYGF10-2F 9. Strain TYGF10-2F9 was determined to produce 5.97 μ g/mL IAA at 100 μ g/mL by constructing an IAA standard curve (fig. 3); has the capability of producing siderophores (Table 2), but does not have the capability of dissolving phosphorus, potassium, fixing nitrogen and producing ACC (Table 2).
TABLE 2 evaluation of growth promoting Properties of the strain TYGF10-2F9
Example 4 Strain TYGF10-2F9 biofilm formation assay
A single colony was inoculated into a 250mL Erlenmeyer flask containing 100mL of LB liquid medium and cultured overnight at 30 ℃ at 160 rpm/min. mu.L of LB, LBG (containing 1% glycerol (v/v)), LBM (containing 0.1mM MnSO) and the like were added to 16-well plates, respectively4) And LBGM (containing 1% glycerol (v/v) and 0.1mM MnSO4) And (3) taking 2 mu L of overnight culture liquid, slightly dripping the liquid onto the liquid surfaces of different culture media, carrying out static culture on a 16-hole plate at the temperature of 30 ℃ for 3d, observing the biofilm formation condition, and recording.
The result of the biomembrane measurement of the strain TYGF10-2F9 shows that: the strain can form obvious biomembranes in LBGM culture medium, the culture medium LBG is next to the strain, and no obvious biomembrane is formed in LBG and LB culture medium (figure 4).
Example 5 potted-plant control of Sclerotinia sclerotiorum by Strain TYGF10-2F9
Firstly, the strain stored at-80 ℃ is streaked on an LB solid medium (tryptone 10g, yeast extract 5g, NaCl 10g, agar 20g, distilled water 1000mL, pH 7.2-7.4), and cultured in a thermostat at 28 ℃ for 24 hours for activation. Then, the activated strain was inoculated with one needle into a 250mL conical flask containing 100mL of LB liquid medium (tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000mL, pH 7.2-7.4)). Performing shake culture at 160rpm/min and 28 deg.C for 48h, centrifuging the fermentation broth for 5min at 10000g, collecting supernatant as thallus-removed fermentation broth, and resuspending thallus with equal amount of sterile water to obtain bacterial suspension. The bacterial suspension and the fermentation liquor are stored in a refrigerator at 4 ℃ for later use.
Respectively using small watering can to make strain fermentation liquor and strain suspension (10)8CFU/mL) is uniformly sprayed on the surfaces of rape seedling leaves, and the hypha blocks of sclerotinia sclerotiorum with the diameter of 3mm are inoculated after the seedlings are slightly dried, and the treatment of spraying 500 mu g/mL carbendazim is used as a medicament control. Repeating 2 pots of seedlings for each treatment, selecting 2 leaves with similar sizes for each seedling, stabbing the center of each leaf by a sterilized syringe needle, and then inoculating. After inoculation, the culture was incubated at 20 ℃ for keeping moistureAnd measuring the diameter of the lesion after 3d, and calculating the inhibition rate.
Inhibition ═ control lesion diameter-treated lesion diameter)/control lesion diameter × 100%
The pot culture control effect evaluation of the sclerotinia rot of colza is carried out on the strain TYGF10-2F9 with broad-spectrum activity. The results show that the strain TYGF10-2F9 has no significant activity (IR is 2.69%) on sclerotium rapae, and the control effect (IR is 80.99%) of the fermentation broth on sclerotium rapae is significantly higher than that (IR is 64.91%) of the medicament control carbendazim (FIG. 5 and FIG. 6).
Example 6 potted-plant control of cucumber fusarium wilt with the strain TYGF10-2F9
Firstly, the strain stored at-80 ℃ is streaked on an LB solid medium (tryptone 10g, yeast extract 5g, NaCl 10g, agar 20g, distilled water 1000mL, pH 7.2-7.4), and cultured in a thermostat at 28 ℃ for 24 hours for activation. Then, the activated strain was inoculated with one needle into a 250mL conical flask containing 100mL of LB liquid medium (tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000mL, pH 7.2-7.4)). Performing shake culture at 160rpm/min and 28 deg.C for 48h, centrifuging the fermentation broth for 5min at 10000g, collecting supernatant as thallus-removed fermentation broth, and resuspending thallus with equal amount of sterile water to obtain bacterial suspension. The bacterial suspension and the fermentation liquor are stored in a refrigerator at 4 ℃ for later use.
Sowing the cucumber seeds in a seedling tray for seedling cultivation, transplanting the cucumber seeds into seedling pots one seedling after one week of seedling cultivation. After transplanting, when the cucumber grows to 2 leaves and one heart stage, a root irrigation method is adopted for carrying out a control effect determination test. The test treatment comprises the following steps: 1) only 25mL cucumber wilts and an equal amount of sterile water (FOC) were inoculated; 2) simultaneously inoculating 25mL cucumber wilt and an equal amount of bacterial suspension (10)8CFU/mL) (F9-C-FOC); 3) simultaneously inoculating 25mL of cucumber withered and equal amount of sterile fermentation liquid (F9-F-FOC); 4) inoculation of bacterial suspension only (10)8CFU/mL) (F9-C); 5) inoculating only the sterile fermentation liquor (F9-F); 6) 25mL of cucumber wilt and an equivalent amount of carbendazim agent (500. mu.g/mL) (Car.) were inoculated simultaneously with 25mL of sterile water (CK). Each treatment was repeated 4 times. And (4) counting disease indexes, plant heights and fresh weights after inoculation for one month, and calculating prevention effects according to the disease indexes.
Cucumber wilting disease index:
level 0: no disease symptoms;
level 1: leaf surfaces below plant 1/4 appeared withered, with no symptoms at the base of the stem, and the plants grew normally;
and 2, stage: the leaves of the plants 1/4-1/2 are withered, the stem base part 1/2 is browned, and the plants are dwarfed;
and 3, level: the leaf surface above 1/2 of the plant shows withering symptom, the stem base part 1/2 of the plant shows brown stain, and the plant is obviously dwarfed;
4, level: the whole plant withers and dies.
TABLE 3 disease-preventing and growth-promoting effects of strain TYGF10-2F9 on cucumber fusarium wilt
The strain TYGF10-2F9 thallus and the fermentation liquor have obvious control effect on cucumber fusarium wilt, the control effect is respectively 66.67 percent and is higher than the control effect (50.00 percent) of medicament contrast carbendazim (Table 3, figure 7). In addition, compared with a blank control, the chlorophyll content (28.68) of the fermentation liquor treated by the strain TYGF10-2F9 is obviously higher than that of a control chlorophyll (26.60), and the thallus treatment is lower than that of the control; the plant height (67.25cm) of cucumber plants treated by the strain TYGF10-2F9 fermentation liquor is obviously higher than that (64.45cm) of a control plant, and the strain treatment is not obviously different from that (64.13cm) of the control plant; the stem thicknesses of the strain TYGF10-2F9 thallus and the cucumber plant treated by the fermentation liquor are both higher than that of a blank control cucumber plant (4.35cm), and are respectively 4.94cm and 4.63 cm; the fresh weight of the cucumber plant treated by the strain TYGF10-2F9 thallus and the fermentation liquor is obviously higher than that of a blank control cucumber plant (17.62g), and is respectively 21.67g and 21.27 g; the weight average of the dry weight of the strain TYGF10-2F9 thallus and the fermentation liquor treated cucumber plant is higher than that of the blank control cucumber plant (2.12g), and the weight average is 2.49g and 2.37g respectively. It can be seen that the strain TYGF10-2F9 thallus and fermentation liquor have a certain growth promoting effect on cucumber plants (Table 3).
The preferred embodiments of the present disclosure are described in detail with reference to the accompanying drawings, however, the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present disclosure within the technical idea of the present disclosure, and these simple modifications all belong to the protection scope of the present disclosure.
It should be noted that, in the foregoing embodiments, various features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various combinations that are possible in the present disclosure are not described again.
In addition, any combination of various embodiments of the present disclosure may be made, and the same should be considered as the disclosure of the present disclosure, as long as it does not depart from the spirit of the present disclosure.
Claims (9)
1. A strain of Bacillus belgii is characterized in that the preservation number of the Bacillus belgii (Bacillus velezensis) is CGMCC No. 21318.
2. The use of bacillus beijerinckii according to claim 1 for the preparation of a plant bacteriostatic agent or a medicament for the control of plant diseases caused by phytopathogens.
3. The use according to claim 2, wherein the phytopathogen is selected from the group consisting of Sclerotinia sclerotiorum (sclerotiorum), Rhizoctonia solani (Rhizoctonia solani), gibberella zeae (Fusarium graminearum), verticillium pomorum (botryospora bereniana), gibberella tabacum (alternaria alternata), Phytophthora capsici (Phytophthora capsici), Fusarium oxysporum f.
4. Use of the Bacillus belgii of claim 1 for the preparation of a plant colonizer, a plant biocontrol agent, a bio-organic fertilizer or a plant growth promoter.
5. A preparation, wherein the preparation is a bacterial suspension, fermentation broth, fermentation product or secondary metabolite of Bacillus belgii of claim 1.
6. The use of a formulation according to claim 5 for the preparation of a plant bacteriostatic agent or a medicament for the control of plant diseases caused by phytopathogens.
7. The use according to claim 6, wherein the phytopathogen is selected from the group consisting of Sclerotinia sclerotiorum (sclerotiorum), Rhizoctonia solani (Rhizoctonia solani), gibberella zeae (Fusarium graminearum), verticillium pomorum (botryospora bereniana), gibberella tabacum (alternaria alternata), Phytophthora capsici (Phytophthora capsici), Fusarium oxysporum f.
8. A method for producing IAA, which comprises using the Bacillus belgii of claim 1 to produce IAA.
9. A process for producing a siderophore characterized by comprising using the Bacillus belgii of claim 1.
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