CN109738622B - Lateral flow paper strip rapid detection device based on micro-fluidic chip - Google Patents
Lateral flow paper strip rapid detection device based on micro-fluidic chip Download PDFInfo
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Abstract
The invention discloses a device for rapidly detecting a lateral flow paper slip based on a microfluidic chip. In a miniaturized microfluidic volume quantification device, the volume of a detection sample is regulated and controlled through various structural modules; the potential pollution of redundant samples to the detection environment is overcome through the waste liquid cavity. The sample is smoothly released to the lateral flowing paper strip through the sample releasing module. In the hand-held image detection and processing device, two LED lamps with specific wavelengths are used as excitation light sources to illuminate the lateral flow paper strips, a miniature camera is used for collecting images of a detection area on the lateral flow paper strips, real-time processing of the detection images is realized through a control circuit board mutually integrated with the miniature camera, gray values of a test line and a control line on the lateral flow paper strips are extracted, and qualitative detection results are displayed on an LCD display screen. The detection device realizes simple, convenient and low-cost lateral flow detection.
Description
Technical Field
The invention relates to the field of biomedical detection, in particular to a lateral flow paper strip rapid detection device based on a microfluidic chip.
Background
The micro-fluidic chip can realize the automatic processing of samples through an integrated micro-channel structure, such as sample introduction, volume quantification, sample pretreatment, and even a series of biomedical detection related operations and processing in a reaction process, detection signal reading and the like, and provides a reasonable solution for realizing the simplification, the convenience and the rapidity of biomedical detection and even reducing the detection cost.
As a simple and convenient rapid disease diagnosis method, after the lateral flow paper strip finishes one-time sample adding, the detection result can be rapidly read after waiting for a certain time, so the method is widely applied to a plurality of field rapid detection environments. However, since there is no sample preprocessing capability, a professional medical detection tool and a professional operation flow, such as a pipette gun and a pipette for quantifying and adding a sample, are often required to complete the detection of the lateral flow paper slip, which often limits the applicable environment and scope of the lateral flow paper slip. On the other hand, the traditional lateral flow paper strips, especially, the detection reading values of the colloidal gold paper strips are often read by human eyes, so that the interference and influence of subjective factors on detection results are easily introduced, and the detection accuracy is often reduced especially in weak positive detection.
The device for rapidly detecting the lateral flow paper slip based on the microfluidic chip disclosed by the invention realizes the sample pretreatment of the lateral flow paper slip detection by virtue of the microfluidic device, reduces the dependence of the detection process on specialized medical detection tools and detection processes, and realizes the integrated, rapid, simple and convenient lateral flow paper slip detection. On the other hand, interference of human eye result interpretation can be overcome by the miniaturized lateral flow paper strip signal reading device, and detection accuracy is improved. Therefore, the micro-fluidic sample adding and volume quantifying device, the miniaturized signal reading device and the lateral flow paper strip are combined with each other, so that the environmental adaptability of lateral flow paper strip detection is further improved, and quick detection of diseases of communities, families and even individuals is realized.
Disclosure of Invention
The invention relates to a lateral flow paper strip rapid detection device based on a microfluidic chip, which comprises two parts: one is a miniaturized volume quantifying device, the other is a miniaturized handheld image detection and processing device, and the two devices are matched with each other to realize the rapid detection of the lateral flow paper strips. The whole process is as follows: firstly, the paper slip is clamped into the clamping groove, then a sample is quantitatively collected and reacts on the paper slip, then the image processing button is pressed, an image is collected through the camera, the signal is detected and analyzed, and the detection result is displayed on the screen.
Wherein, miniaturized micro-fluidic volume proportioning device is including anti-overflow groove 4, filtration membrane 5, inflow channel 6, ration chamber 11, ration chamber outflow channel 5, waste liquid chamber 12, valve 8, draw-in groove inflow channel 20, draw-in groove b21, and the outside transparent film that still has simultaneously for whole device can form airtight space.
The chip overflow-preventing groove 4, the filtering membrane 5, the inflow channel 6, the quantitative cavity 11, the quantitative cavity outflow channel 19 and the waste liquid cavity 12 form a fluid channel, a sample is injected into the clamping groove b21 through the fluid channel, and the immunochromatography reaction is completed by the lateral flow paper strips.
The hand-held image detection and processing device is made of a PMMA polymethyl methacrylate plate. The device shell is pure black and opaque and comprises an image processing button 18, a display screen 1, a circuit board 17, a camera 15, a card slot a9, an image acquisition area 10, a main power switch 7, a light source a13, a light source b14, a USB channel cover 2, a USB channel 3, a connecting device 17, an image processing switch 18 and an embedded slot 22.
The slip has a slip display portion 24 and a sample application hole 23.
There is an anti-overflow groove 4 in the chip structure, and it is located micro-fluidic chip top sample entrance, and there is a filtration membrane 5 sample entrance, and filtration membrane 5 is located inflow channel 6, and liquid gets into ration chamber 11 through inflow channel 6 drainage, realizes the injection function of sample through this structure, simultaneously, filters all kinds of impurity in will detecting the sample through filtration membrane.
The micro-fluidic chip realizes the volume quantification of the sample through the quantification cavity 11, and the left side of the quantification cavity is connected with the waste liquid cavity 12 for storing redundant samples. Below the dosing chamber there is a dosing chamber outflow channel 19. According to the flowing process of sample introduction, the quantitative cavity 11 and the waste liquid cavity 12 are matched with each other, so that accurate sample quantification and storage of surplus samples are realized.
The lower part of the quantitative cavity outflow channel 19 is connected with a clamping groove inflow channel 20 and a microfluidic chip clamping groove b21, the microfluidic chip clamping groove b21 is used for placing a sample adding hole 23 of a lateral flow paper slip, such as a fluorescent paper slip, a valve 8 is arranged between the clamping groove inflow channel 20 and the quantitative cavity outflow channel 19, and the valve 8 controls the on-off of the outflow channel through a slide way.
The device shell is made of PMMA (polymethyl methacrylate) plates, black opaque materials are arranged on the device shell, an image processing button 18 and a display screen 1 are arranged at the top of the device, a USB channel cover 2 and a USB channel 3 are arranged on the side edges, a main power switch 7 is arranged at the bottom of the device, and a clamping groove a9 is arranged right below the device; the device is internally provided with a camera 15, a light source a13, a light source b14, an image acquisition area 10, a circuit board 16 and a circuit board screen connecting device 17. The image processing is finished by one key, and the method is simple and practical. The design of the embedded groove 22 facilitates the disassembly and assembly of the whole device.
The light source a13 and the light source b14 are located at the middle position inside the device and provide illumination when taking a picture, so that the picture taking effect is improved. The camera 15 is positioned right above the inside of the apparatus and faces the image acquisition area 10. The circuit board 16 and the connecting device 17 thereof are positioned at the top inside the device, and the structure is used for accurately shooting required pictures, so that the data volume of image processing is reduced, and the detection efficiency is improved.
The image processing button 18 and the display screen 1 are positioned on the top of the outer layer of the device, the USB channel 2 and the USB channel cover 3 are positioned on the outer side face of the device, and the USB channel cover 3 is closed. It is at the same level with the circuit board 16, there is a main switch 7 of power below it, this design guarantees the dark environment when the battery is powered, this switch-key control device is the only power, easy and simple to handle.
The bottom of the device is provided with a clamping groove a9 inlet which is a paper slip inserting hole, the clamping groove a9 and the clamping groove b21 are correspondingly communicated, a paper slip is tightly matched with the device through the clamping groove a9, after the paper slip is inserted, the paper slip detection reaction part 24 is positioned in the image acquisition area 10, the sample adding hole 23 is over against the clamping groove inflow channel 20 of the micro-fluidic chip, the structure is used for ensuring that the sample accurately enters the sample adding hole 23 when the sample is added, the sample accurately enters the sample adding hole 23, the leakage is prevented from polluting the reaction environment, the integrated design of the device simplifies the operation process, and the integrated operation from the reaction process to the detection of image analysis.
In order to meet the integrated rapid detection requirement of the device, after the picture is shot and obtained, the image characteristics can be rapidly analyzed to obtain an accurate detection result, the invention compiles an image processing algorithm, and an image processing algorithm functional module realizes the result interpretation of the lateral flow paper strip and comprises a preprocessing module, a positioning module and a reading value module. The preprocessing module firstly obtains a color image of the lateral flow paper slip, then divides the image into a target detection area so as to achieve the purpose of reducing an image analysis area, grays the image, removes noise points by using high-pass filtering, and smoothes the image. After the image preprocessing is finished, a positioning module is used for finding and positioning the boundaries of the control lines and the test lines; specifically, searching is started from the Left side of a target detection area, a Left border of a control line is searched by using an a (x, y) template with a Left longitudinal middle pixel point M (M,0) as a reference point, absolute values of convolution sums of gray values of all points at a (x, y) position on an image and a Sobel longitudinal operator Gy are sequentially calculated, the absolute values of the convolution sums of all points on the template are summed and recorded as S (i), the position with the maximum value of S (i) is taken as a Left border position, and an abscissa is recorded as C _ Left; then, by using the same method and taking (C _ Left, 0) as a reference point, matching an a (x, y) template with the Soble in a search area (C _ Left, C _ Left +30) to obtain a control line Right boundary C _ Right; similarly, after the left and right boundaries are determined, respectively finding the upper boundary C _ Up and the lower boundary C _ Down upwards and downwards by using a b (x, y) template and a Sobel transverse operator convolution summation method by taking (C _ left, 0) as a reference point, and finishing the retrieval of the controlled boundaries; and sequentially detecting the searched rectangular frames with the length and the width of the control line area as templates rightwards in the search area, and calculating the sum of gray values of all the points on the rectangular frames. In the search area, the abscissa of the Left boundary point of the rectangular frame at the sum maximum value is recorded as T _ Left, the Right boundary is recorded as T _ Right, and the lower boundary on the test line is consistent with the control line boundary, so that the detection of the test line boundary is completed. And the value reading module reads the gray values of the control line area and the test line area according to the obtained boundary positioning result to obtain the detection result of the lateral flow paper strip, and the result is stored and displayed on the display screen 1. The algorithm completes the analysis and result display of the image, has good rapidity, is free from the influence of artificial subjective factors, has accurate detection result, can save the result in a file, and is beneficial to document management.
Compared with the prior art, the invention has the following beneficial effects.
1. The micro-fluidic chip adopts a disposable detection mode, thereby avoiding the pollution and leakage possibly caused by the original sample in the sample quantitative process in the common detection.
2. The device automatically completes a plurality of reaction steps of quantification, paper slip detection, image detection and processing by matching a miniaturized volume quantification device and a handheld image detection and processing device in an automatic and flow-type working mode to obtain a reaction result of a sample; compared with the traditional mode, the device has the advantages of small volume, simple structure, simple operation, high automation degree, high extraction efficiency and the like, and can be combined with the existing method to form an integrated microfluidic detection system.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the embodiments or the drawings used in the description will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive labor.
FIG. 1 is a schematic view of one embodiment of the overall apparatus of the present invention;
FIG. 2 is a reverse side view of the overall apparatus of the present invention;
FIG. 3 a microfluidic chip;
FIG. 4 is a flow chart of image processing;
in the figure: 1. display screen, 2, USB passageway lid, 3, USB passageway, 4, anti-overflow groove, 5, filtration membrane, 6, inflow passageway, 7, power master switch, 8, valve, 9, draw-in groove a, 10, image acquisition district, 11, solution chamber, 12, waste liquid chamber, 13, light source a, 14, light source b, 15, camera, 16, circuit board, 17, circuit board and display screen connecting device, 18, image processing button, 19, ration chamber outflow passageway, 20, draw-in groove inflow passageway, 21, draw-in groove b, 22, embedded groove, 23, application of sample hole, 24, detection reaction portion.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise.
Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description.
Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate.
In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be discussed further in subsequent figures.
As shown in figure 1, firstly, the lateral flow fluorescent paper strip is inserted from the clamping groove a9, at this time, the paper strip is combined with the miniaturized volume quantifying device and the handheld image detection and processing device, the sample adding hole 23 of the paper strip is fixed in the clamping groove b21, urine is injected from the injection channel 6 through the burette, the flow and release speed of the urine is slow under the action of the filtering membrane 5, the urine is temporarily gathered in the anti-overflow groove 4 and slowly sinks to the quantifying cavity 11 along the slope, and the redundant urine flows into the waste liquid cavity 12 along the triangular slope. After stabilization, the valve 8 is pushed to the bottom, at which point the sample will flow into the well 23. The USB cover 2 is opened, a USB cable is inserted along the USB channel 3, the USB supplies power to the device, the power supply main switch 7 is turned on, the image processing button 18 is pressed, the light source a13 and the light source b14 are lighted, a specific excitation light source is generated, and the lateral flow fluorescent paper strip displays the reaction result. At the same time, the camera 15 starts to take a picture, the detection reaction part 24 positioned in the image acquisition area 10 is shot, the camera 15 transmits the content to the circuit board 16, the image processing is carried out in the circuit board 16, and then the result is transmitted to the display screen 1 by the connecting device 17, and the detection result is displayed. The detection device realizes the sample volume quantification in the lateral flow note detection process by means of the microfluidic chip, and realizes signal reading and analysis through the handheld image detection and processing device, so that the simple, convenient and low-cost lateral flow detection is realized.
The invention relates to a device for rapidly detecting a lateral flow paper slip based on a microfluidic chip, which can be used for detecting urine, serum and the like, and adopts an integrated and running-water type operation mode to realize automatic extraction of a sample. Through the mutual cooperation between the microfluidic chip and the driving device, a plurality of reaction steps such as quantitative detection, image processing and the like are automatically completed, and the measurement result can be quickly, automatically and simply obtained.
The drawings and the embodiments are only for purposes of illustrating the invention and are not to be construed as limiting the invention. It is within the scope of the invention to cover such minor variations within the spirit and scope of the invention as defined by the appended claims. Such as the material, shape and size of the microfluidic chip, the shape and size of the waste liquid cavity, the quantitative cavity, the shape and size of the plug, the shape and size of various functional and connective channels, the shape and size of the chip tray, and the like.
It will be understood by those skilled in the art that all or part of the steps for implementing the above embodiments may be implemented by hardware, or may be implemented by a program instructing relevant hardware, where the program may be stored in a computer-readable storage medium, and the storage medium may be a read-only memory, a magnetic disk or an optical disk.
The description of the present invention has been presented for purposes of illustration and description, and is not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.
Claims (9)
1. Based on quick detection device of micro-fluidic chip side direction class note, its characterized in that: the device comprises a miniaturized volume quantifying device and a handheld image detection and processing device; the whole device is made of PMMA plates; the shell of the image processing device is black PMMA, and the microfluidic device is made of transparent PMMA;
the miniaturized microfluidic volume quantifying device comprises an anti-overflow groove (4), a filtering membrane (5), an inflow channel (6), a quantifying cavity (11), an outflow channel (19) of the quantifying cavity, a waste liquid cavity (12), a valve (8), a clamping groove inflow channel (20), a clamping groove b (21) and a transparent film outside, so that a complete closed system is constructed;
the anti-overflow groove (4), the filtering membrane (5), the inflow channel (6), the quantitative cavity (11), the quantitative cavity outflow channel (19) and the waste liquid cavity (12) form a fluid channel, a sample is injected into the clamping groove b (21) through the fluid channel, and the immunochromatography reaction is completed by the lateral flow paper strips;
the handheld image detection and processing device comprises an image processing button (18), a display screen (1), a circuit board (17), a camera (15), a card slot a (9), an image acquisition area (10), a main power switch (7), a light source a (13), a light source b (14), a USB channel cover (2), a USB channel (3), a screen circuit board connecting device (17), an image processing switch (18) and an embedded slot (22);
the paper slip is provided with a paper slip detection reaction part (24) and a sample adding hole (23);
the quantitative cavity outflow channel (19) below is connected with a clamping groove inflow channel (20), a valve (8) and a clamping groove b (21), the clamping groove b (21) is used for placing a sample adding hole (23) of a lateral flow paper strip, the valve (8) in the middle of the clamping groove inflow channel (20) and the quantitative cavity outflow channel (19) can control the on-off of the outflow channel through a slide way, when the valve (8) is pushed to the bottom end, a sample is released into the sample adding hole (23), and the sample releasing module is structurally designed to enable the sample volume to be quantitatively added to the lateral flow paper strip.
2. The microfluidic chip-based lateral flow paper strip rapid detection device of claim 1, wherein: there is an anti-overflow groove (4) in the chip structure, and it is located micro-fluidic chip top sample entrance, and there is a filtration membrane (5) sample entrance, and filtration membrane (5) are located inflow channel (6), and liquid gets into ration chamber (11) through inflow channel (6) drainage, realizes the appearance function of advancing of sample through this structure, simultaneously, filters all kinds of impurity in the sample that will detect through filtration membrane.
3. The microfluidic chip-based lateral flow paper strip rapid detection device of claim 1, wherein: the microfluidic chip realizes the volume quantification of a sample through a quantification cavity (11), and the left side of the quantification cavity is connected with a waste liquid cavity (12) for storing redundant samples; when the sample is excessive, the excessive sample flows into the waste liquid cavity (12) from the quantitative cavity (11) along the triangular slope between the two parts, and the triangular slope ensures that the excessive sample quickly and accurately flows into the waste liquid cavity (12); a quantitative cavity outflow channel (19) is arranged below the quantitative cavity; according to the flowing process of sample introduction, the quantitative cavity (11) and the waste liquid cavity (12) are matched with each other, so that accurate sample quantification and storage of surplus samples are realized.
4. The microfluidic chip-based lateral flow paper strip rapid detection device of claim 1, wherein: the USB channel cover (2) and the USB channel (3) are positioned on the side edges, a main power switch (7) is arranged at the bottom, and a card slot a (9) is arranged right below the device; the device is internally provided with a camera (15), a light source a (13), a light source b (14), an image acquisition area (10) and a circuit board (16); the device has two power supply modes, one mode is that USB power supply is adopted, and the USB is inserted into a circuit board (16) along a USB channel (3); the other is battery powered, with the battery being directly connected to the circuit board (16).
5. The microfluidic chip-based lateral flow paper strip rapid detection device of claim 1, wherein: the light source a (13) and the light source b (14) are positioned in the middle position in the device and provide illumination when in shooting so as to improve the shooting effect; the camera (15) is positioned right above the inside of the device and is opposite to the image acquisition area (10); the circuit board (16) and the connecting device (17) thereof are positioned on the top of the interior of the device, and the reaction detection area of the lateral flow paper strip is accurately shot through the structural design.
6. The microfluidic chip-based lateral flow paper strip rapid detection device of claim 1, wherein: the image processing button (18) and the display screen (1) are positioned at the top of the outer layer of the device, the USB channel (2) and the USB channel cover (3) are positioned on the outer side surface of the device, and the USB channel cover and the circuit board (16) are positioned on the same horizontal plane; when the USB is used for supplying power, the USB is inserted along the USB channel (2), and the USB is just tightly attached to the USB channel (2) at the moment, so that an external light source is prevented from entering, and the design ensures the dark environment in the device; when the battery is used for supplying power, the USB channel cover (3) is directly closed, so that a dark environment is ensured when the battery supplies power; a power main switch (7) is arranged below the outer side surface of the device, and the switch controls the unique power supply of the device.
7. The microfluidic chip-based lateral flow paper strip rapid detection device of claim 1, wherein: the bottom of the device is provided with a clamping groove a (9) inlet which is a paper slip inserting port, and a clamping groove b (21) is positioned at the lower part of the microfluidic chip and is used for placing a sample adding hole of a lateral flow paper slip; the clamping groove a (9) is correspondingly communicated with the clamping groove b (21), the paper slip is tightly matched with the device through the clamping groove a (9), after the paper slip is inserted, the detection reaction part (24) is positioned in the image acquisition area (10), and the sample adding hole (23) is over against the clamping groove inflow channel (20) of the microfluidic chip, so that the structure not only ensures that the sample accurately enters the sample adding hole (23) when the sample is added, prevents leakage from polluting the reaction environment, but also ensures that the detection reaction part (24) is displayed in the image acquisition area (10), and the detection position is accurate; meanwhile, the integrated design of the device simplifies the operation process and realizes the integrated operation from the reaction process to the detection image analysis.
8. The microfluidic chip-based lateral flow paper strip rapid detection device of claim 1, wherein: the image processing algorithm functional module realizes result interpretation of the lateral flow paper strips and comprises a preprocessing module, a positioning module and a reading module; the preprocessing module firstly acquires a lateral flow paper strip color image, then divides the image into a target detection area to achieve the purpose of reducing an image analysis area, grays the image, removes noise points by using high-pass filtering and smoothes the image; after the image preprocessing is finished, a positioning module is used for finding and positioning the boundaries of the control lines and the test lines; specifically, searching is started from the Left side of a target detection area, a Left boundary of a control line is searched by using an a (x, y) template with a Left longitudinal middle pixel point M (M,0) as a reference point, the absolute value of the convolution sum of the gray value of all points at the position of the a (x, y) on an image and a Sobel longitudinal operator Gy is sequentially calculated, the absolute value of the convolution sum of all points on the template is summed, S (i) is recorded, the position with the maximum value of S (i) is taken as the position of the Left boundary, the abscissa is recorded as C _ Left, and then the same method is used, the (C _ Left, 0) is taken as the reference point, and the a (x, y) template and Soble are matched in a search area (C _ Left, C _ Left +30) to obtain the Right boundary C _ Right of the control line; similarly, after the left and right boundaries are determined, respectively finding the upper boundary C _ Up and the lower boundary C _ Down upwards and downwards by using a b (x, y) template and a Sobel transverse operator convolution summation method by taking (C _ left, 0) as a reference point, and then finishing the retrieval of the controlled boundaries; sequentially detecting the searched rectangular frame with the length and the width of the control line area as a template to the right in the search area, and calculating the sum of gray values of all points on the rectangular frame; in the search area, recording the abscissa of the Left boundary point of the rectangular frame at the sum maximum value as T _ Left, recording the Right boundary as T _ Right, and finishing the detection of the boundary of the test line, wherein the upper and lower boundaries of the test line are consistent with the boundaries of the control lines; and the value reading module reads the gray values of the control line area and the test line area according to the obtained boundary positioning result to obtain the detection result of the lateral flow paper strip, and the result is stored and displayed on the display screen (1).
9. The microfluidic chip-based lateral flow paper strip rapid detection device of claim 1, wherein: firstly, a lateral flow paper slip is inserted from a clamping groove a (9), at the moment, the paper slip is combined with a miniaturized volume quantifying device and a handheld image detection and processing device, a sample adding hole (23) of the paper slip is fixed in a clamping groove b (21), a sample is injected from an inflow channel (6) through a burette, the flowing and releasing speed of the sample is slow under the action of a filtering membrane (5), the sample is temporarily gathered in an anti-overflow groove (4) and slowly sinks into a quantifying cavity (11) along a slope, and redundant sample flows into a waste liquid cavity (12) along a triangular slope; after stabilization, the valve (8) is pushed to the bottom, and the sample flows into the sample adding hole (23); opening the USB cover (2), inserting a USB wire along the USB channel (3), wherein the USB supplies power to the device, turning on the power main switch (7), pressing the image processing button (18), wherein the light source a (13) and the light source b (14) are lightened, and the lateral paper flow strip displays the reaction result; simultaneously, the camera (15) starts to take a picture, a detection reaction part (24) positioned in the image acquisition area (10) is shot, the camera (15) transmits the content to the circuit board (16), the image processing is carried out in the circuit board (16), and then the result is transmitted to the display screen (1) by the connecting device (17).
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| CN111458502A (en) * | 2020-03-08 | 2020-07-28 | 北京化工大学 | Microfluidic HIV urine detection device |
| CN111337683B (en) * | 2020-03-24 | 2024-02-09 | 北京万泰生物药业股份有限公司 | Portable HIV detection device |
| CN114376619A (en) * | 2020-10-20 | 2022-04-22 | 中国计量大学 | Multi-index female ovulation detection system |
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