EPRS

EPRS1
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1FYJ, 4HVC, 4K86, 4K87, 4K88, 5A34, 5BMU, 5A1N, 5A5H
Identifiers
Aliases	EPRS1, EARS, GLUPRORS, PARS, QARS, QPRS, PIG32, glutamyl-prolyl-tRNA synthetase, HLD15, glutamyl-prolyl-tRNA synthetase 1, EPRS
External IDs	OMIM: 138295; MGI: 97838; HomoloGene: 5870; GeneCards: EPRS1; OMA:EPRS1 - orthologs
Gene location (Human)
Chr.	Chromosome 1 (human)
End	220,046,530 bp
Gene location (Mouse)
Chr.	Chromosome 1 (mouse)
End	185,160,557 bp
RNA expression pattern
	Top expressed in
	parotid gland; ; optic nerve; ; secondary oocyte; ; Achilles tendon; ; lateral nuclear group of thalamus; ; ventricular zone; ; tibia; ; islet of Langerhans; ; external globus pallidus; ; Brodmann area 23;
	Top expressed in
	otic placode; ; Rostral migratory stream; ; parotid gland; ; saccule; ; maxillary prominence; ; human fetus; ; mandibular prominence; ; tail of embryo; ; genital tubercle; ; otic vesicle;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	nucleotide binding; protein binding; RNA stem-loop binding; ATP binding; catalytic activity; ligase activity; aminoacyl-tRNA ligase activity; GTPase binding; RNA binding; glutamate-tRNA ligase activity; proline-tRNA ligase activity; zinc ion binding; protein homodimerization activity; metal ion binding; identical protein binding;
Cellular component	membrane; GAIT complex; cytoplasm; aminoacyl-tRNA synthetase multienzyme complex; cytosol; plasma membrane; ribonucleoprotein complex;
Biological process	regulation of translation; tRNA aminoacylation; metabolism; protein biosynthesis; tRNA aminoacylation for protein translation; negative regulation of translation; cellular response to interferon-gamma; glutamyl-tRNA aminoacylation; prolyl-tRNA aminoacylation; cellular response to insulin stimulus; long-chain fatty acid import into cell; protein-containing complex assembly; regulation of long-chain fatty acid import into cell;
	Sources:Amigo / QuickGO
Orthologs
	2058
	107508
	ENSG00000136628
	ENSMUSG00000026615
	P07814
	Q8CGC7
	NM_004446
	NM_029735; NM_001357474
	NP_004437
	NP_084011; NP_001344403
	Wikidata
View/Edit Human	View/Edit Mouse

Bifunctional aminoacyl-tRNA synthetase is an enzyme that in humans is encoded by the EPRS gene.^[5]^[6]

Gene

Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.^[6]

Function

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.^[6]

Phosphorylation of EPRS is reported to be essential for the formation of GAIT (Gamma-interferon Activated Inhibitor of Translation) complex that regulates the translation of multiple genes in monocytes and macrophages.^[7]

Interactions

EPRS has been shown to interact with POU2F1,^[8] Heat shock protein 90kDa alpha (cytosolic), member A1^[9] and IARS.^[10]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000136628 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000026615 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Fett R, Knippers R (February 1991). "The primary structure of human glutaminyl-tRNA synthetase. A highly conserved core, amino acid repeat regions, and homologies with translation elongation factors". J Biol Chem. 266 (3): 1448–55. doi:10.1016/S0021-9258(18)52315-2. PMID 1988429.
^ ^a ^b ^c "Entrez Gene: EPRS glutamyl-prolyl-tRNA synthetase".
^ Arif A, Jia J, Mukhopadhyay R, Willard B, Kinter M, Fox PL (July 2009). "Two-site phosphorylation of EPRS coordinates multimodal regulation of noncanonical translational control activity". Mol. Cell. 35 (2): 164–80. doi:10.1016/j.molcel.2009.05.028. PMC 2752289. PMID 19647514.
^ Nie J, Sakamoto S, Song D, Qu Z, Ota K, Taniguchi T (March 1998). "Interaction of Oct–1 and automodification domain of poly(ADP-ribose) synthetase". FEBS Lett. 424 (1–2): 27–32. doi:10.1016/S0014-5793(98)00131-8. PMID 9537509. S2CID 872132.
^ Kang J, Kim T, Ko Y G, Rho S B, Park S G, Kim M J, Kwon H J, Kim S (October 2000). "Heat shock protein 90 mediates protein-protein interactions between human aminoacyl-tRNA synthetases". J. Biol. Chem. 275 (41): 31682–8. doi:10.1074/jbc.M909965199. ISSN 0021-9258. PMID 10913161.
^ Rho SB, Lee J S, Jeong E J, Kim K S, Kim Y G, Kim S (May 1998). "A multifunctional repeated motif is present in human bifunctional tRNA synthetase". J. Biol. Chem. 273 (18): 11267–73. doi:10.1074/jbc.273.18.11267. ISSN 0021-9258. PMID 9556618.

Kaiser E, Eberhard D, Knippers R (1992). "Exons encoding the highly conserved part of human glutaminyl-tRNA synthetase". J. Mol. Evol. 34 (1): 45–53. Bibcode:1992JMolE..34...45K. doi:10.1007/BF00163851. PMID 1556743. S2CID 6021527.
Norcum MT (1991). "Structural analysis of the high molecular mass aminoacyl-tRNA synthetase complex. Effects of neutral salts and detergents". J. Biol. Chem. 266 (23): 15398–405. doi:10.1016/S0021-9258(18)98629-1. PMID 1651330.
Cerini C, Kerjan P, Astier M, et al. (1992). "A component of the multisynthetase complex is a multifunctional aminoacyl-tRNA synthetase". EMBO J. 10 (13): 4267–77. doi:10.1002/j.1460-2075.1991.tb05005.x. PMC 453179. PMID 1756734.
Kunze N, Bittler E, Fett R, et al. (1990). "The human QARS locus: assignment of the human gene for glutaminyl-tRNA synthetase to chromosome 1q32-42". Hum. Genet. 85 (5): 527–30. doi:10.1007/BF00194231. PMID 2227938. S2CID 27487450.
Thömmes P, Fett R, Schray B, et al. (1988). "The core region of human glutaminyl-tRNA synthetase homologies with the Escherichia coli and yeast enzymes". Nucleic Acids Res. 16 (12): 5391–406. doi:10.1093/nar/16.12.5391. PMC 336774. PMID 3290852.
Kaiser E, Hu B, Becher S, et al. (1994). "The human EPRS locus (formerly the QARS locus): a gene encoding a class I and a class II aminoacyl-tRNA synthetase". Genomics. 19 (2): 280–90. doi:10.1006/geno.1994.1059. PMID 8188258.
Hillier LD, Lennon G, Becker M, et al. (1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi:10.1101/gr.6.9.807. PMID 8889549.
Rho SB, Lee JS, Jeong EJ, et al. (1998). "A multifunctional repeated motif is present in human bifunctional tRNA synthetase". J. Biol. Chem. 273 (18): 11267–73. doi:10.1074/jbc.273.18.11267. PMID 9556618.
Quevillon S, Robinson JC, Berthonneau E, et al. (1999). "Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein". J. Mol. Biol. 285 (1): 183–95. doi:10.1006/jmbi.1998.2316. PMID 9878398.
Kang J, Kim T, Ko YG, et al. (2000). "Heat shock protein 90 mediates protein-protein interactions between human aminoacyl-tRNA synthetases". J. Biol. Chem. 275 (41): 31682–8. doi:10.1074/jbc.M909965199. PMID 10913161.
Jeong EJ, Hwang GS, Kim KH, et al. (2001). "Structural analysis of multifunctional peptide motifs in human bifunctional tRNA synthetase: identification of RNA-binding residues and functional implications for tandem repeats". Biochemistry. 39 (51): 15775–82. doi:10.1021/bi001393h. PMID 11123902. S2CID 25514243.
Sang Lee J, Gyu Park S, Park H, et al. (2002). "Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex". Biochem. Biophys. Res. Commun. 291 (1): 158–64. doi:10.1006/bbrc.2002.6398. PMID 11829477.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Sampath P, Mazumder B, Seshadri V, et al. (2004). "Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation". Cell. 119 (2): 195–208. doi:10.1016/j.cell.2004.09.030. PMID 15479637. S2CID 18944876.
Kato T, Daigo Y, Hayama S, et al. (2005). "A novel human tRNA-dihydrouridine synthase involved in pulmonary carcinogenesis". Cancer Res. 65 (13): 5638–46. doi:10.1158/0008-5472.CAN-05-0600. PMID 15994936.
Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

This article on a gene on human chromosome 1 is a stub. You can help Wikipedia by expanding it.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000136628 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000026615 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid1988429-5] Fett R, Knippers R (February 1991). "The primary structure of human glutaminyl-tRNA synthetase. A highly conserved core, amino acid repeat regions, and homologies with translation elongation factors". J Biol Chem. 266 (3): 1448–55. doi:10.1016/S0021-9258(18)52315-2. PMID 1988429.

[entrez-6] "Entrez Gene: EPRS glutamyl-prolyl-tRNA synthetase".

[pmid19647514-7] Arif A, Jia J, Mukhopadhyay R, Willard B, Kinter M, Fox PL (July 2009). "Two-site phosphorylation of EPRS coordinates multimodal regulation of noncanonical translational control activity". Mol. Cell. 35 (2): 164–80. doi:10.1016/j.molcel.2009.05.028. PMC 2752289. PMID 19647514.

[pmid9537509-8] Nie J, Sakamoto S, Song D, Qu Z, Ota K, Taniguchi T (March 1998). "Interaction of Oct–1 and automodification domain of poly(ADP-ribose) synthetase". FEBS Lett. 424 (1–2): 27–32. doi:10.1016/S0014-5793(98)00131-8. PMID 9537509. S2CID 872132.

[pmid10913161-9] Kang J, Kim T, Ko Y G, Rho S B, Park S G, Kim M J, Kwon H J, Kim S (October 2000). "Heat shock protein 90 mediates protein-protein interactions between human aminoacyl-tRNA synthetases". J. Biol. Chem. 275 (41): 31682–8. doi:10.1074/jbc.M909965199. ISSN 0021-9258. PMID 10913161.

[pmid9556618-10] Rho SB, Lee J S, Jeong E J, Kim K S, Kim Y G, Kim S (May 1998). "A multifunctional repeated motif is present in human bifunctional tRNA synthetase". J. Biol. Chem. 273 (18): 11267–73. doi:10.1074/jbc.273.18.11267. ISSN 0021-9258. PMID 9556618.

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Gene

Function

Interactions

References

Further reading