Cytoskeleton-associated protein 2 is a protein that in humans is encoded by the CKAP2 gene .[ 5] [ 6]
Human CKAP2 gene, the cDNA of which is known as LB1, is a cytoskeleton -associated protein involved in mitotic progression. Its high transcriptional activity has been observed in the testes , thymus , and diffuse B-cell lymphomas . The gene codes for a protein of 683 residues, which lacks a homology to known amino acid sequences. On evidence of immunofluorescence analysis, the CKAP2 product is a cytoplasmic protein associated with cytoskeletal fibrils.
The CKAP2 gene is in chromosome 13q 14. Rearrangements of this region result in various tumors. Thus deletions have been detected in multiple myeloma , prostate cancer , head-and-neck squamous-cell carcinoma , B-cell prolymphocytic leukemia , non-Hodgkin lymphoma , and in more than half cases of B-cell chronic lymphocytic leukemia .
^ a b c GRCh38: Ensembl release 89: ENSG00000136108 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000037725 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Maouche-Chretien L, Deleu N, Badoual C, Fraissignes P, Berger R, Gaulard P, Romeo PH, Leroy-Viard K (Oct 1998). "Identification of a novel cDNA, encoding a cytoskeletal associated protein, differentially expressed in diffuse large B cell lymphomas". Oncogene . 17 (10): 1245–51. doi :10.1038/sj.onc.1202048 . PMID 9771967 . S2CID 24883661 . ^ "Entrez Gene: CKAP2 cytoskeleton associated protein 2" .
Alternative titles: tumor- and microtubule-associated protein; TMAP, LB1
Eichmuller S, Usener D, Dummer R, et al. (2001). "Serological detection of cutaneous T-cell lymphoma-associated antigens" . Proc. Natl. Acad. Sci. U.S.A . 98 (2): 629–34. doi :10.1073/pnas.021386498 PMC 14639 PMID 11149944 . Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs" . Genome Res . 11 (3): 422–35. doi :10.1101/gr.GR1547R . PMC 311072 PMID 11230166 . Udina IG, Baranova AV, Kompaniĭtsev AA, Sulimova GE (2001). "Evolutionarily-conserved gene CKAP2, located in region 13q14.3 of the human genome, is frequently rearranged in various tumors". Genetika . 37 (1): 120–3. PMID 11234418 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Rakhmanaliev ER, Klimov EA, Kompaniĭtsev AA, Sulimova GE (2003). "[The structure of the human oncogenesis-associated CKAP2 (LB1) gene]". Mol. Biol. (Mosk.) . 36 (6): 985–9. PMID 12500535 . Bae CD, Sung YS, Jeon SM, et al. (2004). "Up-regulation of cytoskeletal-associated protein 2 in primary human gastric adenocarcinomas". J. Cancer Res. Clin. Oncol . 129 (11): 621–30. doi :10.1007/s00432-003-0484-0 . PMID 12942315 . S2CID 22334193 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Dunham A, Matthews LH, Burton J, et al. (2004). "The DNA sequence and analysis of human chromosome 13" . Nature . 428 (6982): 522–8. Bibcode :2004Natur.428..522D . doi :10.1038/nature02379 . PMC 2665288 PMID 15057823 . Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization" . Curr. Biol . 14 (16): 1436–50. Bibcode :2004CBio...14.1436J . doi :10.1016/j.cub.2004.07.051 PMID 15324660 . S2CID 2371325 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle" . Proc. Natl. Acad. Sci. U.S.A . 103 (14): 5391–6. Bibcode :2006PNAS..103.5391N . doi :10.1073/pnas.0507066103 PMC 1459365 PMID 16565220 . Jeon SM, Choi B, Hong KU, et al. (2006). "A cytoskeleton-associated protein, TMAP/CKAP2, is involved in the proliferation of human foreskin fibroblasts". Biochem. Biophys. Res. Commun . 348 (1): 222–8. doi :10.1016/j.bbrc.2006.07.046 . PMID 16876122 . Hong KU, Park YS, Seong YS, et al. (2007). "Functional Importance of the Anaphase-Promoting Complex-Cdh1-Mediated Degradation of TMAP/CKAP2 in Regulation of Spindle Function and Cytokinesis" . Mol. Cell. Biol . 27 (10): 3667–81. doi :10.1128/MCB.01386-06 . PMC 1900002 PMID 17339342 . Seki A, Fang G (2007). "CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit" . J. Biol. Chem . 282 (20): 15103–13. doi :10.1074/jbc.M701688200 PMID 17376772 .
