File:Selective-Detection-of-NADPH-Oxidase-in-Polymorphonuclear-Cells-by-Means-of-NAD(P)H-Based-602639.f1.ogv

FITC stained conidia (green, small, round) and hyphae (green, ribbon-shaped). The movie is a time series of 3D fluorescence images recorded every 60 seconds Note that the PMNs interact with hyphae both along their length (center) as well as with their conidiophores (right corner), that is, the part of hypha responsible for the asexual reproduction. Movie 2: The movie is a time series of fluorescence lifetime images of enzyme-bound NAD(P)H of PMNs interacting with an aspergillus hypha recorded every 60 seconds Note that only the PMNs, which contact the hypha shows an increased fluorescence lifetime specific for the NADPH bound to NADPH oxidase. Moreover, this specific fluorescence lifetime appears only during the contact between cell and fungus, meaning that it appears and disappears a