File:A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s009.ogv

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A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s009.ogv (Ogg Theora video file, length 30 s, 512 × 512 pixels, 256 kbps, file size: 936 KB)

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English: Live cell imaging of ricin-FITC in the presence of JJX12 and TGN. Real time imaging of A549 cells visualizing ricin-FITC in green pre-mixed with JJX12 prior to adding to cells and TGN in red labeled with a transient transfection as described in Material and Methods. The total fluorescence for the TGN marker is always overwhelming compared to ricin-FITC and therefore the absolute co-localization between ricin-FITC and the TGN is not apparent in the raw images prior to processing. Real time images were processed prior to quantification as described in Materials and Methods to determine co-localization values, which are reported in Fig 4B. Total length of the time lapse is 120 min.
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Source S3 Movie from Herrera C, Klokk T, Cole R, Sandvig K, Mantis N (2016). "A Bispecific Antibody Promotes Aggregation of Ricin Toxin on Cell Surfaces and Alters Dynamics of Toxin Internalization and Trafficking". PLOS ONE. DOI:10.1371/journal.pone.0156893. PMID 27300140. PMC: 4907443.
Author Herrera C, Klokk T, Cole R, Sandvig K, Mantis N
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current02:17, 13 October 201630 s, 512 × 512 (936 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 83 kbps Completed 15:44, 14 February 2023 4.0 s
VP9 360P 44 kbps Completed 15:44, 14 February 2023 2.0 s
VP9 240P 23 kbps Completed 15:44, 14 February 2023 1.0 s
WebM 360P 154 kbps Completed 15:44, 14 February 2023 1.0 s
QuickTime 144p (MJPEG) 32 kbps Completed 07:38, 14 October 2024 1.0 s

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