File:A-Beta-Herpesvirus-with-Fluorescent-Capsids-to-Study-Transport-in-Living-Cells-pone.0040585.s005.ogv
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
No higher resolution available.
A-Beta-Herpesvirus-with-Fluorescent-Capsids-to-Study-Transport-in-Living-Cells-pone.0040585.s005.ogv (Ogg Theora video file, length 9.9 s, 220 × 220 pixels, 2.8 Mbps, file size: 3.31 MB)
File information
Structured data
Captions
Summary
[edit]DescriptionA-Beta-Herpesvirus-with-Fluorescent-Capsids-to-Study-Transport-in-Living-Cells-pone.0040585.s005.ogv |
English: (in support of Fig. 7 and Figure S3) Tracing of extracellular MCMV trafficking. M2-10B4 cells were seeded at low density on glass-bottomed culture dishes and infected at a MOI of 100 with gradient purified S-GFP-SCP virus stock. Directly after infection fluorescence live cell imaging with 488 nm laser excitation in parallel with differential interference contrast (DIC) microscopy was started in an environmentally controlled chamber. Virus particles attached to cell protrusions were identified by their fluorescence and recorded with 1.8 frames per second. The video is an overlay of the DIC and GFP channels. |
||
Date | |||
Source | Video S1 from Bosse J, Bauerfeind R, Popilka L, Marcinowski L, Taeglich M, Jung C, Striebinger H, von Einem J, Gaul U, Walther P, Koszinowski U, Ruzsics Z (2012). "A Beta-Herpesvirus with Fluorescent Capsids to Study Transport in Living Cells". PLOS ONE. DOI:10.1371/journal.pone.0040585. PMID 22792376. PMC: 3394720. | ||
Author | Bosse J, Bauerfeind R, Popilka L, Marcinowski L, Taeglich M, Jung C, Striebinger H, von Einem J, Gaul U, Walther P, Koszinowski U, Ruzsics Z | ||
Permission (Reusing this file) |
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 05:05, 18 November 2012 | 9.9 s, 220 × 220 (3.31 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Bosse J, Bauerfeind R, Popilka L, Marcinowski L, Taeglich M, Jung C, Striebinger H, von Einem J, Gaul U, Walther P, Koszinowski U, Ruzsics Z |
---|---|
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | (in support of Fig. 7 and Figure S3) Tracing of extracellular MCMV trafficking. M2-10B4 cells were seeded at low density on glass-bottomed culture dishes and infected at a MOI of 100 with gradient purified S-GFP-SCP virus stock. Directly after infection fluorescence live cell imaging with 488 nm laser excitation in parallel with differential interference contrast (DIC) microscopy was started in an environmentally controlled chamber. Virus particles attached to cell protrusions were identified by their fluorescence and recorded with 1.8 frames per second. The video is an overlay of the DIC and GFP channels. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |