Immunoglobulin functional motions and their effects on the complementarity determining regions
Authors: 
Michael T. Zimmermann, Aris Skliros, Saras Saraswathi, Andrzej Kloczkowski, Robert L. JerniganAuthors Info & Claims
Michael T. Zimmermann, Aris Skliros, Saras Saraswathi, Andrzej Kloczkowski, Robert L. JerniganAuthors Info & ClaimsPages 621 - 626
Abstract
Motions of the IgG structure are evaluated using normal mode analysis and a new time dependent form of the elastic network model, to detect hinges, the dominance of low frequency modes, and the most important internal motions. We also evaluate the protein crystal and its packing effects on the experimental temperature factors and disorder prediction. We find that the effects of the protein environment on the crystallographic temperature factors may be misleading for evaluating specific functional motions of IgG. The extent of motion of the antigen binding domains is computed to show their large spatial sampling. We conclude that the IgG structure is specifically designed to facilitate large excursions of the antigen binding domains. Normal modes are shown as capable of computationally evaluating the hinge motions and the spatial sampling of domains of the structure. The antigen binding loops and the major hinge appear to behave similarly to the rest of the structure when we consider the dominance of the low frequency modes and the extent of internal motion.
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Index Terms
- Immunoglobulin functional motions and their effects on the complementarity determining regions
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Published In
August 2010
705 pages
ISBN:9781450304382
DOI:10.1145/1854776
- General Chairs:
- Aidong Zhang,
- Mark Borodovsky,
- Program Chairs:
- Gultekin Ozsoyoglu,
- Armin Mikler
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Published: 02 August 2010
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