Tierra Biosciences’ Post

For those of you interested in proteins and Cell-Free expression, go check out our latest blog, it is a great piece of scientific communication! #CFPS #proteins #biochemistry

Protein Expression Market Size, Share & Trends Analysis Report By Expression system (Bacterial, Yeast, Insect, Mammalian, Cell-free systems), By Application (Therapeutic proteins, Research applications, Industrial enzymes, Biologics production), By End User (Biotechnology and pharmaceutical companies, Academic and research institutions, Contract research organizations (CROs), Others), Covid-19 Impacts, Global Economy Insights, Regional Outlook, Growth Potential, Price Trends, Competitive Market Share & Forecast, 2023 - 2031. 𝐈𝐌𝐈𝐑 𝐌𝐚𝐫𝐤𝐞𝐭 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡. #Note: The above outline provides a general structure for the content. You may modify and expand it based on the specific requirements and available information. 📚𝐆𝐞𝐭 𝐓𝐡𝐢𝐬 𝐑𝐞𝐩𝐨𝐫𝐭 𝐀𝐭 𝐃𝐢𝐬𝐜𝐨𝐮𝐧𝐭𝐞𝐝 𝐏𝐫𝐢𝐜𝐞:📝👇https://lnkd.in/dzcEARyE   📚𝐑𝐞𝐩𝐨𝐫𝐭 𝐅𝐨𝐜𝐮𝐬𝐞𝐝 𝐎𝐧 𝐓𝐨𝐩 𝐂𝐨𝐦𝐩𝐚𝐧𝐢𝐞𝐬:👇 ABclonal Technology Agilent Technologies Ajinomoto Bio-Pharma Services ARTES Biotechnology GmbH Aviva Systems Biology Axxam Becton Dickinson Company (Safety Syringes, Inc. ) BioBetter™ BioChain Institute, Inc. Biognosys BioLinker Bio-Rad Laboratories BPS Bioscience Inc. Combined Therapeutics Correlia Biosystems Expression Systems GenScript JOINN Biologics US Inc. LifeSensors, Inc Lonza Lucigen Corp. Merck KGaA, Darmstadt, Germany New England Biolabs ORF Genetics Orna Therapeutics Oxford Expression Technologies Peak Proteins Pepmic Co., Ltd ProMab Biotechnologies, Inc. Promega Corporation Protein Sciences Corporation ProteinSimple ProteoGenix QIAGEN Replicate Bioscience, Inc. Sigma-Aldrich Sino Biological, Inc. Strand Therapeutics Synbio Technologies

Plant cell culture. 1. Light: The requirements on light conditions of plant cells cultured in vitro are not very strict because the nutrition for cell growth is mainly provided from media. However, light is not only related to photosynthesis but also to cell differentiation, for example, photoperiod can regulate sex cell differentiation and flowering. Thus in the cell culture for the purpose of obtaining plants, the light condition is very important. In the process of obtaining important substances such as drugs by culturing plant cells in vitro, plant cells are mostly cultured in suspension in a reactor. 2. Hormones: The division and growth of plant cells particularly require the regulation of plant hormones. Auxin, which promotes growth, and mitogen, which promotes cell division, are the most basic hormones. The division, growth, differentiation and individual growth cycles of plant cells are regulated by corresponding hormones. Compared with animal cells, the principles of hormone requirements for plant cell culture in vitro are already understood, and its application technology is also quite mature, and there is already a set of culture media that can be used. At the same time, it solves the needs of plant cells for water, nutrients, hormones, osmotic pressure, pH, trace elements, etc. #cellculture #biology #biological #biotechnology #bioengineering https://www.g-biotec.com/

I am excited to share that a part of my PhD research in the field of media development for biologics manufacturing conducted at The Johns Hopkins University was published this month in Metabolic Engineering Journal. https://lnkd.in/dn_Z-XcM In this study, we demonstrate how the use of Evonik's Ala-Cys-Cys-Ala dipeptide dimer enables the formulation of cysteine-free and cystine-free feed medium, improves glucose metabolism in CHO cell cultures, and boosts mAb production. Given the challenges posed in the production and storage of highly concentrated feed media containing cysteine and cystine and the increasing adoption of intensified fed-batch processes to manufacture biotherapeutics, alternatives to cysteine and cystine such as Ala-Cys-Cys-Ala will pave the way for improved media formulations, enhanced storage stability of media, and satisfy demands of critical amino acids while maintaining bioprocess performance. Thanks to Dr. Betenbaugh, Dr. Antoniewicz, and Dr. Martin Schilling for their mentorship, Advanced Mammalian Biomanufacturing Innovation Center and member companies for funding this research, and Pranay Ladiwala and Xiangchen Cai for the great teamwork. #biotechnology #pharmaceuticals #biomanufacturing #healthcare #upstream #processdevelopment #cellculture #fluxanalysis #13Clabeling

In recent advancements within microbial expression systems, PUREcoli strains are revolutionizing the production of therapeutic proteins and peptides. By enhancing product purity and process efficiency, these engineered strains significantly reduce host cell impurities and extend the plasma half-life of proteins through PASylation. This innovation streamlines manufacturing processes and lowers clinical and commercial risks. #Biotech #MicrobialExpressionSystem #TherapeuticProteins

Exploring Metabolic Effects of Dipeptide Feed Media on CHO Cell Cultures by In Silico Model-Guided Flux Analysis There is a growing interest in perfusion or continuous processes to achieve higher productivity of biopharmaceuticals in mammalian cell culture, specifically Chinese hamster ovary (CHO) cells, towards advanced biomanufacturing. These intensified bioprocesses highly require concentrated feed media in order to counteract their dilution effects. However, designing such condensed media formulation poses several challenges, particularly regarding the stability and solubility of specific amino acids. In this study, we combined one of the lead amino acids, L-tyrosine, which is known for its poor solubility in water due to its aromatic ring and hydroxyl group, with glycine as the partner, thus forming glycyl-L-tyrosine (GY) dipeptide. https://lnkd.in/gAmntDVK #aspenalert #biotech #bioprocess

Instability and limited solubility of amino acids can cause issues when developing media and feeds for mammalian bioprocesses - and this challenge can be increased when the process is continuous or perfusion based. One proposed solution to this is the use of dipeptides, which are formed by combining 2 amino acids together (I.e. for solving this problem, the dipeptide would contain one amino acid that is causing an issue with another). Here, this group explore the use of a Tyr-containing dipeptide (glycol-L-tyrosine; GY). #bioprocessing #biotechnology #cellculturemedia #biopharmaceuticals https://lnkd.in/ed9dwFdt

🚀 ⚡ 💧 💡 Rapid #protein #prototyping: Cell-Free Protein Synthesis (CFPS) can shorten the timeline for drug discovery with automated #workflows that exploit reaction #miniaturization and high-throughput #purification 🚀 ⚡ 💧 💡 Two application notes by New England Biolabs describe such workflows using off-the-shelf reagents 👇 📒 https://lnkd.in/d3-8DwM7 📒 https://lnkd.in/d8V-E5Hf Try to implement it and let me know, I would love to collaborate on this 🙂 #CellFree #SynBio

Synthesis of mono Cytochrome P450 in a modified CHO-CPR cell-free protein production platform https://lnkd.in/eMyqqquM

The Cytiva Protein Select resin, has an accepted peer-reviewed paper out since some days. A good read indeed about this enhanced auto-cleaving ligand-tag system, where no tag amino acid residues are left behind. Tag-traceless product. How cool is not that 💪 Clifford R, et al., (2024) Production of Native Recombinant Proteins Using a Novel Split Intein Affinity Technology. J Chrom A