INTEGRA Biosciences’ Post

🔬🧬 Get ready for the future with a variety of molecular biology Innovations: DNA and RNA isolation, protein isolation, reverse transcription, amplification, cloning, and nucleic acid electrophoresis. #thermofisheremp 📩 Need help? Send me a mail to [email protected] with the topic 'LinkedIn'

First successful electrophoresis chamber was first invented in 1938. Today, electrophoresis can be completed safety in as little as 15 minutes. What are other advancements in molecular biology research? Find out more here: 👇 #iloveMolBio #CelebrateDNA #electrophoresis #MolecularBiology

Explore how automated colorimetric colony selection can streamline your research and enhance precision in genetic screening. 🧬 App Note Highlights: 🌟 Precision Screening: Learn how white light imaging and QPix Chroma Filters differentiate colonies with high accuracy. 🌟 High Throughput: Discover the ability to select over 3,000 colonies per hour, exceeding manual methods. 🌟 Intelligent Software: Understand how QPix Software 2.0 customizes colony selection for precise results. 🌟 DNA Verification: Review the processes that confirm the accuracy of colony selection with DNA sequencing. Gain valuable insights to boost your research efficiency.

Pyro(phospho)mania Understanding the role of pyrophosphorylation requires specific analytical strategies to discriminate it from protein phosphorylation. A custom workflow reveals that nucleolar protein pyrophosphorylation in human cells regulates the transcription of ribosomal DNA. https://lnkd.in/g3pAxRiU

It is commonly known that the #PCR (#PolymeraseChainReaction) is an important technique in #molecularbiology, considering its ability to amplify #DNA sequences. But are you familiar with all the phases involved in this #technique? - Phase I: “Denaturation”, in which high temperatures disrupt #hydrogen bonds between complementary DNA strands, transforming the double-stranded DNA into single strands. - Phase II: “Annealing”, in which #primers bind to their complementary sites on the single-stranded DNA template. In this phase, the reducement in temperature facilitates primer annealing. - Phase III: “Extension”, in which the DNA polymerase extends the primers by adding nucleotides complementary to the template strand, resulting in the #synthesis of two new DNA strands, each complementary to the original template, effectively doubling the DNA content. REFERENCES Bhat, A. I., et al. “Polymerase chain reaction." (2020): 323-345. Delidow, B. C., et al. (1993). “Polymerase Chain Reaction”. In: White, B.A. (eds) PCR Protocols. Methods in Molecular Biology, v. 15. Humana Press, Totowa, NJ. PraxiLabs - Available in: https://lnkd.in/dxDVwq-T

"DreamTaq Green MasterMix" is a product used in molecular biology laboratories. It's a pre-mixed solution containing: 1. DreamTaq DNA polymerase (an enzyme for DNA amplification) 2. dNTPs (building blocks for DNA synthesis) 3. Buffer (a solution for optimal enzyme activity) 4. Magnesium chloride (a cofactor for the enzyme) 5. Green dye (for easy visualization) This master mix is designed for PCR (Polymerase Chain Reaction) experiments, which involve amplifying specific DNA sequences. The green color makes it easy to handle and track the samples.

Southern blotting vs Northern blotting vs PCR Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. ... The major difference is that RNA, rather than DNA, is analyzed in the northern blot. A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. Polymerase chain reaction (PCR) is a method widely used in molecular biology to make multiple copies of a specific DNA segment. Using PCR, a single copy (or more) of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of the particular DNA segment. Youtube video: https://lnkd.in/gJVecD7y #nikolaysgeneticslessons

Week 2 : western blotting Western blotting: it's more than just a fancy name for fingerprinting proteins! This powerful technique is a workhorse in molecular biology, separating and identifying proteins from complex mixtures like cells or tissues. Electrophoresis > transfer > detection

Viscolase: Your Supernatural Solution to Molecular Biology Challenges! Tired of battling DNA/RNA slime in your lab experiments? Viscolase, a genetically engineered nuclease, is here to redefine your molecular biology experience! This Supernatural Slime Buster goes beyond ordinary tools, leaving even renowned options envious. Why choose Viscolase for your molecular biology conundrums? 1. DNA/RNA Removal from Recombinant Protein Parties: Viscolase clears unwanted genetic material, letting your proteins shine like stars. 2. Viscosity Vibe Check in Cell Lysate Parties: Say goodbye to sticky messes! Viscolase reduces viscosity, making cell lysates smoother than a hit song. 3. Preparation of Samples for 2D Gel Electrophoresis: Ready to drop the beat on the 2D gel? Viscolase ensures your protein samples are primed for a molecular masterpiece. Bid farewell to slimy obstacles and embrace Viscolase's supernatural powers. Revolutionize your lab experience and conquer molecular biology challenges like never before! https://lnkd.in/d6dvFFBB #biotechnology #lifescience #viscolase #reduction #viscosity

**XpressDNA Soil Kit:** 🌍 Conquer the complexities of soil DNA extraction with XpressDNA Soil Kit! Our patented Magnetic Nanoparticle technology ensures rapid and high-quality microbial DNA purification. Features include: - Efficient lysis of microorganisms without bead beating 🦠 - Higher yield and purity, ideal for PCR and sequencing 🧬 - Effective removal of PCR inhibitors for metagenomics research 🧪 - Perfect for diverse soil samples, including extreme environments 🏞️ **#SoilDNA #GenomicResearch #MagneticNanoparticles #Metagenomics** #microbiomeresearch #molecularbiology