D2R | DNA to RNA’s Post

A paper has been published in which the rat BBB Kit is used! This study demonstrated that the poliovirus capsid protein VP3 can cross the vascular endothelial cells. two independent parts of VP3 contribute to this function and can enter human umbilical cord vascular endothelial cells and peripheral blood mononuclear cells. the VP3-derived peptide crossed the rat-derived blood brain barrier (BBB) model (BBB Kit™, PharmaCo-Cell, Japan) and migration from the blood side to the brain side was observed. These results provide new insights into the molecular mechanisms by which polioviruses invade the central nervous system (CNS). The BBB Kit is a breakthrough in vitro BBB model developed by PharmCo-Cell, which reconstitutes a BBB by co-culturing three types of BBB-related primary culture cells. The BBB Kit incorporates a high-quality cell culture insert, cellQART® (SABEU). #Bloodbrainbarrier #bbb #bbbkit #brain #endothelialcell #poliovirus #polio #virus #infection #invitromodel #MPS #cellqart #cellcultureinsert #SABEU #pharmacocell https://lnkd.in/g4XhaXKe

Torque Teno Virus (TTV) qPCR has been studied for many years and just recently became available to order at Eurofins Viracor. TTV measures the net state of immune suppression in solid organ transplant patients. Please see the link below to register for the webinar and learn more about how this test is changing IS in transplant patients. #SOT #transplant #immunesuppression

Highly recommended this webinar on this new tool to help personalize solid organ transplant immusuppression!

Today, on World Hepatitis Day, we're reminded that "It's time for action" against viral hepatitis. Every 30 seconds, someone loses their life to a hepatitis-related illness. This sobering statistic underscores the urgent need for continued research, improved diagnostics, and innovative treatments in our collective fight against the viruses. At PhoenixBio, we're committed to supporting this vital cause. Our PXB-mouse, a humanized liver mouse model, for example, enables researchers to study the entire viral life cycle of hepatitis B, hepatitis C, and hepatitis D with high translational relevance. By providing tools that bridge the gap between preclinical and clinical research, we aim to accelerate the development of new therapies and bring hope to millions affected by hepatitis. Together, we can work towards the WHO's goal of eliminating viral hepatitis by 2030. #WorldHepatitisDay #Hepatitis #WorldHealthOrganization

📣 WEBINAR ALERT! Register HERE 👉 https://lnkd.in/gtf7ye3v Save the date for ‘Torque Teno Virus for Immunologic Monitoring’ 📅 12 FEB 2025 | 2-3pm CST Presented by Gregor Bond, MD, PhD. Topics covered include: • Torque teno virus (TTV) is being considered as a biomarker for immune function, as its plasma load reflects the host’s immune status. • TTV load is linked to rejection and infection in solid organ transplant recipients. • Proposed TTV load cut-off values could aid in risk stratification for lung and kidney transplant recipients. #TorqueTenoVirus #Anellovirus #TTViWATCH™ #ViracorTTV #TTV

A paper has been published in which the rat BBB Kit is used! This study demonstrated that the poliovirus capsid protein VP3 can cross the vascular endothelial cells. two independent parts of VP3 contribute to this function and can enter human umbilical cord vascular endothelial cells and peripheral blood mononuclear cells. the VP3-derived peptide crossed the rat-derived blood brain barrier (BBB) model (BBB Kit™, PharmaCo-Cell, Japan) and migration from the blood side to the brain side was observed. These results provide new insights into the molecular mechanisms by which polioviruses invade the central nervous system (CNS). The BBB Kit is a breakthrough in vitro BBB model developed by PharmCo-Cell, which reconstitutes a BBB by co-culturing three types of BBB-related primary culture cells. The BBB Kit incorporates a high-quality cell culture insert, cellQART® (SABEU). #Bloodbrainbarrier #bbb #bbbkit #brain #endothelialcell #poliovirus #polio #virus #infection #invitromodel #MPS #cellqart #cellcultureinsert #SABEU

Senior author Dr. Henry VanBrocklin's research on the persistence of T cell activation and viral RNA after COVID-19 has been highlighted as the cover story on #ScienceTranslationalMedicine. The findings reveal significant immune changes up to 2 years post-infection, providing new perspectives on Long COVID. Learn more about the ongoing impact of COVID-19 and the importance of continued research in this area: https://lnkd.in/eNwt43db

Challenges to mapping and defining m6A function in viral RNA The latest article from the #RNASpecialIssue describes challenges to defining the function of m6A in viral RNA molecules, and provides a framework for future studies to understand how m6A regulates viral infection. Access now: bit.ly/3xY1vZ4 #RNAEpitranscriptomics #ViralRNA #m6A

The Life Cycle of HCV-C In the liver’s domain, where cells unite, A tale unfolds of HCV’s silent flight. With CD81 as its guide, it enters the fray, Receptor-mediated, to find its way. Once inside, it sheds its coat, Releasing RNA, a viral note. Ribosomes dance to the viral score, Synthesizing polyproteins, and more. Protease springs forth, a cleaver keen, Slicing proteins, a viral machine. Cleavage of polyproteins, its crucial role, Releasing functional proteins, to take control. Membranes rearranged, a fortress made, Protecting RNA, in their shade. In the ER and Golgi’s embrace, Virions assemble, with stealth and grace. Through the secretory pathway, they roam, Released into the bloodstream, to find a new home. Host factors intertwine, in a silent design, As HCV exploits, the dance of virus and host entwined. But when protease’s cleaver keen is blocked, The virus’s grip, by inhibition, is shocked. Without its slices, polyproteins remain whole, Viral replication halted, under control. Understanding the cycle, where cells unite, Guides the quest, for a cure’s light. Author’s note: HCV stands for Hepatitis C Virus. ER stands for Endoplasmic Reticulum.

Initiator Cell Death Induced by SARS-CoV-2 in Human Airway Epithelium SARS-CoV-2 triggers multiple cell death pathways, contributing to disease severity. Key findings from Liang et al. include: Primary Mechanism: Necroptosis was identified as the primary cell death mechanism, initiated by viral Z-RNA binding to Z-DNA binding protein-1 (ZBP-1) in ACE2-expressing cells and primary human airway epithelial (HAE) cultures. Apoptosis: Both infected and bystander cells underwent apoptosis, though it was primarily a bystander effect. Variant Comparison: The Delta variant induced significantly more Z-RNA/ZBP-1 interactions and necroptosis than Omicron, correlating with greater disease severity. This study highlights the role of necroptosis in SARS-CoV-2 pathogenesis and the differential impact of virus variants. 🧬🔬💀 #COVID19 #CellDeath #Pathogenesis https://lnkd.in/dHAgDxcJ