🔸𝘾𝙍𝙄𝙎𝙋𝙍-𝘾𝙖𝙨9 𝙏𝙚𝙘𝙝𝙣𝙤𝙡𝙤𝙜𝙮:
✳️CRISPR-Cas9 is a revolutionary gene-editing technology that allows precise modification of DNA in various organisms, including humans. CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats, which are specific DNA sequences found in bacteria. Cas9, on the other hand, is an enzyme that acts like molecular scissors.
✳️The process begins by designing a synthetic RNA molecule, often called guide RNA (gRNA), which matches the target DNA sequence. This gRNA is then combined with the Cas9 enzyme to form a CRISPR-Cas9 complex. When introduced into a cell, this complex searches for the matching DNA sequence. Once found, Cas9 cuts the DNA at the targeted location.
✳️This cut triggers the cell's natural DNA repair mechanisms, leading to modifications such as gene insertions, deletions, or replacements. Scientists can harness this process to edit genes for various purposes, including studying gene function, treating genetic disorders, or creating genetically modified organisms.
✳️CRISPR-Cas9 has garnered widespread attention for its simplicity, efficiency, and versatility, making it a powerful tool in genetic research and potential therapeutic applications. However, ethical and safety considerations accompany its use, sparking ongoing discussions about responsible gene editing.
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Senior Bioinformatician | Single Cell Analysis | Machine Learning | Computational Biology | Functional Genomics | Skilled in Python, R, bash
3moCongrats to the Gilbert Lab!