Anna-Nicole Bosco

I had a great time today with excellent neuroscientists from the mice🐭, monkey🐒, zebrafish🐟 as well as drosophila🪰 field. Thanks for giving me the opportunity to talk about my research in the small circuit & behavior meeting at UPenn. Very helpful and interesting topics. Like it! #Dopamine #Locomotion

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Seeking full time opportunities in the Biotechnology landscape Happy Monday everyone! As I near the completion of my Master's in Biotechnology program at The Johns Hopkins University, I've been reflecting on a journey deeply rooted in scientific curiosity and skill acquisition. Throughout my time at Hopkins, especially in the Andrew Lab at the The Johns Hopkins University School of Medicine, I've delved into exciting projects that demanded innovative thinking and rigorous scientific methods. My work has not only sharpened my skills in 𝐦𝐨𝐥𝐞𝐜𝐮𝐥𝐚𝐫 𝐛𝐢𝐨𝐥𝐨𝐠𝐲 𝐭𝐞𝐜𝐡𝐧𝐢𝐪𝐮𝐞𝐬—such as molecular cloning, PCR, qPCR, cDNA library preparation, Western Blots, and ELISA—but also in exploring 𝐜𝐞𝐥𝐥 𝐜𝐮𝐥𝐭𝐮𝐫𝐞 𝐦𝐨𝐝𝐞𝐥 𝐬𝐲𝐬𝐭𝐞𝐦𝐬 by performing transfection, immunohistochemistry, flow cytometry, and co-immunoprecipitation (Co-IP). My experience has taught me that technical expertise alone isn't enough—it's about how you apply that knowledge towards solving real-world problems. This understanding has been reflective as I hope to transition my skills towards more integrative applications, aiming to bridge the gap between experimental research and therapeutic development. The unpredictability of science teaches that adaptability and curiosity are crucial, qualities that I want to carry into every challenge. Looking ahead, I'm eager to apply this mindset in a role where my efforts will have an impactful impact on patient outcomes, particularly in roles that harness my background to propel cutting-edge research forward. 𝐈'𝐦 𝐜𝐮𝐫𝐫𝐞𝐧𝐭𝐥𝐲 𝐬𝐞𝐞𝐤𝐢𝐧𝐠 𝐟𝐮𝐥𝐥-𝐭𝐢𝐦𝐞 𝐨𝐩𝐩𝐨𝐫𝐭𝐮𝐧𝐢𝐭𝐢𝐞𝐬 where I can contribute effectively, continue to learn and grow within the field of biotechnology. Let's connect if you think my 𝐛𝐚𝐜𝐤𝐠𝐫𝐨𝐮𝐧𝐝 𝐦𝐢𝐠𝐡𝐭 𝐛𝐞 𝐚 𝐟𝐢𝐭 𝐟𝐨𝐫 𝐲𝐨𝐮𝐫 𝐭𝐞𝐚𝐦 or if you have any advice on transitioning into impactful biotech roles. Feel free to check out my profile to learn more about my previous work experiences:) Thank you for being part of my journey, and I look forward to where curiosity leads me next in the pharmaceutical and biotechnology landscape. 🔬 #celltherapy #genetherapy #oncology #hiring #scientist #research #moleculartherapeutics #pharmaceutical #therapeutics

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In the spirit of the recent Postdoc Appreciation Week, I would like to give a shoutout to my Postdoc mentor Priyamvada Acharya, and the fantastic team at The Duke Human Vaccine Institute. I also want to share my research papers resulting from the opportunity to work on some amazing projects: 1. Vaccine induction of CD4-mimicking HIV-1 broadly neutralizing antibody precursors in macaques. Cell - https://lnkd.in/g9WsBKJy 2. Engineering immunogens that select for specific mutations in HIV broadly neutralizing antibodies. bioRxiv [Preprint] - https://lnkd.in/gyHqfsvD 3. Stabilized HIV-1 envelope immunization induces neutralizing antibodies to the CD4bs and protects macaques against mucosal infection. Science Translational Medicine - https://lnkd.in/gxBc46Ec 4. Structural diversity of the SARS-CoV-2 Omicron spike. Molecular Cell - https://lnkd.in/gSRan5tb 5. A broadly cross-reactive antibody neutralizes and protects against sarbecovirus challenge in mice. Science Translational Medicine - https://lnkd.in/gJXhDyHx 6. Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies. Cell Reports - https://lnkd.in/gjj3YuzB 7. Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity. Science - https://lnkd.in/g7rVR4zc 8. In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies. Cell - https://lnkd.in/gJim3965 9. Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses. Nature - https://lnkd.in/gqK5e6Da 10. Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies. Cell - https://lnkd.in/gZ8q9ieS 11. Cold sensitivity of the SARS-CoV-2 spike ectodomain. Nature Structural & Molecular Biology - https://lnkd.in/ganQjryb 12. D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. Cell Reports - https://lnkd.in/gmgHTuZn

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Breakthrough: CRISPR helped to restore vision ♥️ 👁️ Imagine Seeing the World with New Eyes! That's exactly what happened to Olivia Cook, a 22-year-old college student who had been living with a severe form of vision impairment. Thanks to a groundbreaking gene-editing procedure using CRISPR technology, Olivia is experiencing the world in ways she never thought possible! 🔬 This life-changing treatment targeted a genetic mutation responsible for her inherited blindness. The results? For the first time, Olivia saw faces in dim light and enjoyed the simple beauty of Christmas lights twinkling at dusk. 🎉 Promising Results Published! The trial’s outcomes, featured in the New England Journal of Medicine, are promising! Patients like Olivia reported significant improvements in their vision, and what’s even better—there were no serious side effects. 🚀 This breakthrough is more than just a medical success; it's a beacon of hope for many who suffer from genetic diseases. It proves that with science and innovation, the possibilities are endless. Excited to see more such innovations ♥️ #geneediting #crispr Image and news credit: CNN (https://lnkd.in/duK_Q2Bc)

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I'm excited to share that I've had the opportunity to make significant contributions to three research papers in the field of peptide and protein chemistry! The first study, where I was a co-author, introduces 4,4-Difluoroproline (Dfp) as a novel probe for examining the conformational biases of proline in peptides, utilizing 19F NMR spectroscopy. Our findings illuminate how Dfp can be a powerful tool for probing cis-trans isomerism and aiding in the understanding of protein folding and structural order in disordered protein states. Check it out here: https://lnkd.in/ecnd43AG In the second study, where I was the first author, we explored how acyl capping groups affect the stability of α-helices in peptides. Our findings highlight the critical role of hydrogen bonding to water, rather than just electronic effects, in stabilizing helical structures. Read more here: https://lnkd.in/eaYDMe3H In the third study, also where I was the first author, we demonstrated how different acyl capping groups can influence the stability of the polyproline II helix, a vital secondary structure in proteins. This paper, currently under review and published at ChemRxiv, underscores the importance of n->π* interactions and provides new insights into peptide structure modulation through acyl group identity. You can check it out here: https://lnkd.in/eAzFAHwG These studies contribute to a deeper understanding of peptide dynamics, with implications for designing better therapeutic peptides and understanding protein behavior in disease. I'm grateful for the collaborative efforts of my co-authors and mentors who provided immense support and insight throughout these projects.

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After over 15 exciting years at the bench conducting transformative research in the fields of synthetic and molecular biology, I’m exploring new options, focusing particularly on the business side of research. I’ve always had an affinity and knack for Laboratory Operations, in both academic and industry settings, large and small. I’d love to chat about how your organization could benefit from a skilled bench scientist in the ops position.  How has operations impacted your organization and your research? How can we better integrate research and operations to allow for more efficient and impactful science?

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