Leica DM500 User Manual

Leica DM500 User Manual Manufacturer Information Published in January 2015 by: Leica Microsystems (Schweiz) AG Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) In case of questions, please contact the hotline: [email protected] Leica DM500 User Manual 2 Chapter Overview Safety regulations 5 The Leica DM500 16 Get Ready! 19 Get Set! 26 Go! 35 Care of the Microscope 37 Dimensions 40 Leica DM500 User Manual 3 Contents Safety Regulations Safety Concept Symbols Used in This Operating Manual Important Notes Instructions on Use Health Risks and Dangers of Use Information for the Person Responsible for the Instrument Care Instructions Accessories, Maintenance and Repair Electrical Data and Ambient Conditions The Leica DM500 Congratulations! Unpacking Get Ready! Attaching the Viewing Tubes Leica EZ Viewing Tube – Integrated Eyepieces Leica Standard Viewing Tube – Separated Eyepieces Eyeguards Installing Objectives and Substage Condenser Leica DM500 User Manual 6 7 8 9 11 Get Set! Turning on the Microscope Using the Condenser Prepare to View a Specimen Slide Focusing Viewing Tube Adjustment Oil Immersion Technique 27 28 29 30 31 33 12 13 14 15 Go! Ready! Set! Go! 36 Care of the Microscope General Maintenance 38 Dimensions Dimensions 41 17 18 20 21 23 24 25 4 Safety Regulations Leica DM500 User Manual Safety Regulations 5 Safety Concept The individual modules of the Leica DM microscopy series include an interactive CD-ROM with all relevant user manuals several languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our website at www.leica-microsystems.com. Before installing, operating or using the instruments, read the user manuals listed above. In particular, please observe all safety instructions. To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals. This operating manual describes the special functions of the individual modules of the Leica DM microscopy series and contains important instructions for their operational safety, maintenance, and accessories. The "Safety concept" booklet contains additional safety information regarding the service work, requirements and the handling of microscope, accessories and electrical accessories as well as general safety instructions. You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier. Leica DM500 User Manual Safety Regulations 6 Symbols Used in This Operating Manual Warning of a danger This symbol indicates especially important information that is mandatory to read and observe. Failure to comply can cause the following: Hazards to personnel O O Functional disturbances instruments or damaged Warning of hazardous electrical voltage This symbol indicates especially important information that is mandatory to read and observe. Failure to comply can cause the following: O Hazards to personnel O Danger due to hot surface This symbol warns against touching accessible hot surfaces, e.g. those of light bulbs. Important information This symbol indicates additional information or explanations that intend to provide clarity. Explanatory notes f This symbol within the text points to additional information and explanations. Instructions for disposing of the instrument, accessory components, and consumables. Functional disturbances or damaged instruments Leica DM500 User Manual Safety Regulations 7 Important Notes Description The Leica DM500 microscope meets today's state of the art of technology. Nevertheless, hazards may still arise during operation. The potential risks are described below. Before installing, operating or using the instruments, it is required to read this user manual. In particular, please observe all safety instructions. • User manual This user manual includes important instructions related to operating safety, maintenance and accessories. Your Leica DM500 microscope comes with an interactive CD-ROM with all relevant user manuals. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our website at www.leica-microsystems.com. Accessories from third-party suppliers You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier. Original condition To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals. Legal requirements Adhere to general and local regulations relating to accident prevention and environmental protection. EC Declaration of Conformity Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity. Leica DM500 User Manual Safety Regulations 8 Instructions on Use The Leica DM500 microscope may be used only in closed rooms and must be placed on a solid substrate. The Leica DM500 microscope can be used in clean rooms without any problems. Always position the Leica DM500 microscope so that you can disconnect it from the power supply at any time. The power cable must remain accessible at all times, because it is intended as a power disconnect device. Leica DM500 User Manual Place of use Only use the instruments in closed, dust free rooms and between +10°C and +40°C. Protect the devices from oil, chemicals and extreme humidity. If using the devices outdoors, protect them from dust and moisture. Never use electrical devices outdoors. Install electrical devices at least 10 cm from the wall and away from lammable substances. Non-intended use Never install any other plug or unscrew any mechanical components unless expressly instructed to do so in the instructions. The devices and accessories described in this instruction manual have been tested for safety and potential hazards. Avoid large temperature luctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images. The responsible Leica ailiate must be consulted whenever the instrument is altered, modiied or used in conjunction with non-Leica components that are outside of the scope of this manual! In warm and warm-damp climatic zones, the individual components require special care in order to prevent the build-up of fungus. Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims. Safety Regulations 9 Instructions on Use (Continued) Transport If at all possible, use the original packaging for shipping or transporting individual modules. In order to prevent damage from vibrations, disassemble all moving parts that (according to the user manual) can be assembled and disassembled by the customer and pack them separately. Leica DM500 User Manual Disposal Once the product has reached the end of its service life, please contact Leica Service or Sales about disposal. Please observe and ensure compliance with the national laws and regulations that implement, for example, the EC Directive WEEE. Integration in third-party products When installing Leica products into third-party products, the manufacturer of the complete system or its dealer is responsible for following all applicable safety instructions, laws and guidelines. Like all electronic devices, this instrument, its accessory components and consumables must never be disposed of with general household waste. Disposal must comply with locally applicable laws and regulations. Safety Regulations 10 Health Risks and Dangers of Use Health risks Workplaces with microscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal problems may occur. For this reason, appropriate measures for reduction of the workload must be taken: O Optimum workplace layout O Frequent changes of activity O Danger of infection Direct contact with eyepieces is a potential transmission method for viral infections of the eye. • Dangers during use O The Leica DM500 microscope may only be connected to a grounded socket. O The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups. The Leica DM500 microscope may not be operated unless it is in proper functioning condition. The microscope illumination is in the exempt group (risk group 0) according to EN 62471:2008 when used according to its intended use. Never look directly into the LED beam • of the illumination equipment – either with or without optical instruments – as this increases the risk class. Failure to observe this notice poses a risk of eye damage. Thorough training of the personnel, giving consideration to ergonomic and organizational aspects The ergonomic design and construction of the Leica microscopy series are intended to reduce the exertion of the user to a minimum. Leica DM500 User Manual Safety Regulations 11 Information for the Person Responsible for the Instrument Information for the person responsible for the instrument O Ensure that the Leica DM500 microscope is used only by qualiied personnel. O O O O O O Ensure that this user manual is always available at the Leica DM500 microscope. Carry out regular inspections to make certain that the authorized users are adhering to safety requirements. O When instructing new users, do so thoroughly and explain the meanings of the warning signs and messages. Assign individual responsibilities for starting, operating and servicing the instrument and monitor the observance of these responsibilities. Leica DM500 User Manual O Do not use the Leica DM500 microscope unless it is in perfect condition. Inform your Leica representative or Leica Microsystems (Schweiz) AG, 9435 Heerbrugg, Switzerland, immediately of any product defect that could potentially cause injury or harm. If you use accessories made by third-party manufacturers with the Leica DM500 microscope, be sure that each such manufacturer conirms the safety-engineering, harmless usability of the product and observe the product's user manual. Modiications and maintenance of the Leica DM500 microscope may only be performed by professionals expressly authorized by Leica. Safety Regulations O O O O Only original Leica replacement parts may be used in servicing the product. After service work or technical modiications, the unit must be reconigured with observance to our technical requirements. If the unit is modiied or serviced by unauthorized persons, is improperly maintained (as long as maintenance was not carried out by us), or is handled improperly, Leica will not accept any liability. The electric installation in the building must conform to the national standard, e.g. current-operated ground leakage protection (fault-current protection) is suggested. 12 Care Instructions General instructions O Protect the Leica DM500 microscope against damp, vapors, acids, alkalis, and corrosive substances. Do not store chemicals in the vicinity. O O O Protect the Leica DM500 microscope from oil and grease. Never grease or oil mechanical parts or sliding surfaces. Follow the instructions of the disinfectant manufacturer. It is advisable to enter a service agreement with Leica Service. Leica DM500 User Manual Cleaning coated parts and plastic parts Dust and dirt particles should be removed with a soft brush or lint-free cotton cloth. O O O O Remove coarse debris with a moistened disposable cloth. Acetone, xylene or nitro-containing thinners must NOT be used. Cleaning glass surfaces Remove dust using a dry and grease-free brush made from hair, by blowing with bellows, or by using a vacuum. O O Optical surfaces should be cleaned with a lint-free cloth, lens tissue, or cotton swab moistened with a commercially available glass cleaner. Never use chemicals to clean colored surfaces or accessories with rubberized parts. This could damage the surfaces, and specimens could be contaminated by abraded particles. Safety Regulations 13 Accessories, Maintenance and Repair The Leica accessories described in this user manual. Maintenance The Leica DM500 microscope is basically maintenance-free. To ensure that it always operates safely and reliably, we recommend that you take the precaution of contacting the responsible service organization. Other accessories, provided that these have been expressly approved by Leica as being technically safe in this context. You can arrange for periodic inspections or, if appropriate, conclude a maintenance contract with them. Accessories Only the following accessories may be used with the Leica DM500 microscope: O O O O O Leica DM500 User Manual It is advisable to enter a service agreement with Leica Service. For maintenance and repair, only OEM spare parts may be used. Safety Regulations Repairs and service work O Only original Leica Microsystems spare parts may be used. O O Before opening the instruments, switch of the power and unplug the power cable. Avoid contact with powered electrical circuits, which can lead to injury. Service address In case of problems, please contact us as follows: [email protected] 14 Electrical Data and Ambient Conditions Fuse replacement Unplug the instrument before changing any fuses. The Leica DM500 has two fuses, which are located behind the power cord receptacle. • Use only fuse type: 5×20 mm, 1 Amp/250 V, Quick acting (# 13RFAG30003) Electrical data Leica DM500 Input: 100–240 VAC, 50/60 Hz, 5 W (3 W LED) Use only 1.2 Volt NiMH type AA rechargeable batteries with a maximum of 2700 mAh. Do not use non-rechargeable AA batteries. • Leica DM500 User Manual Environment Temperature for use +10 °C … +40 °C Storage temperature -20 °C … +52 °C +50 °F … +104 °F Manipulation shock 25 mm on 50 mm hard wood Transport shock (unpacked) 100 g / 6 ms Transport shock (packed) 800 mm free fall Transport vibrations (unpacked) 5–200 Hz / 1.5 g Atmospheric pressure "in use" and for storage 500–1060 mBar Humidity “in use” and for storage 20–90 % Installation Category II (Overvoltage Category) Pollution degree 2 Safety Regulations 15 The Leica DM500 Leica DM500 User Manual The Leica DM500 16 Congratulations! Thank you for purchasing the Leica DM500 Compound Microscope from Leica Microsystems. This model’s exclusive design features and full range of accessories make it a truly versatile, high-quality instrument. Leica DM500 User Manual The Leica DM500 17 Unpacking O O O O Carefully remove the microscope and any components from the packing carton. Check the components against the planned coniguration. Optional items such as contrast accessories, camera adapters, cameras, and carrying cases are not shipped as part of the standard equipment. These items are delivered in separate packages. Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise. Leica DM500 User Manual The Leica DM500 18 Get Ready! Leica DM500 User Manual Get Ready! 19 Attaching the Viewing Tubes Tools used O Allen key There are two types of viewing tube. Identify which viewing tube you have: Type 1: Leica EZ viewing tube with integrated eyepieces 1. Slightly unscrew the set screw (located on top of the stand) with the Allen key tool provided. 2. Insert the dovetail into the stand support and tighten the set screw irmly without forcing it. This precisely positions the viewing tube onto the optical axis of the microscope, regardless of the selected rotation. Type 2: Standard viewing tube with separate eyepieces Leica DM500 User Manual Get Ready! 20 Leica EZ Viewing Tube – Integrated Eyepieces 1. To use the captive thumbscrew, completely remove the set screw that comes with the stand. 2. Position the Leica EZ viewing tube back in place on the stand. Leica EZ viewing tube with integrated eyepieces To rotate the Leica EZ viewing tube, either loosen the set screw on the stand or replace the set screw with the (optional) captive thumbscrew. Leica DM500 User Manual Be sure the thumbscrew portion of the captive thumbscrew is fully loosened before attaching it to the stand: • Get Ready! 3. Attach the captive thumbscrew using the wrench tool that comes with the captive thumbscrew. 21 Leica EZ Viewing Tube – Integrated Eyepieces (Continued) You can now safely rotate the Leica EZ viewing tube by loosening the captive thumbscrew, rotating the viewing tube, and tightening the captive thumbscrew again. The eyepieces are already integrated with the Leica EZ viewing tube and are pre-adjusted for corrected vision; therefore no eyepiece adjustment or insertion is necessary. Go on to the section "Eyeguards" on page 24. Leica DM500 User Manual Get Ready! 22 Leica Standard Viewing Tube – Separated Eyepieces 1. Insert the eyepieces into the eyetubes. 2. Lock the eyepieces into the eyetubes by tightening the silver screws on the underside of the eyetubes with a standard Cross type (Phillips) screwdriver (not provided). Standard viewing tube; eyetubes do not yet contain the eyepieces The standard viewing tube has a rotatable dovetail. Therefore, you can now rotate the standard viewing tube freely in any orientation. The eyepieces will still rotate, but they will be captive in the eyetubes. Leica DM500 User Manual Get Ready! 23 Eyeguards If you wear eyeglasses for microscope viewing, keep the rubber eyeguards folded down. If you do not wear eyeglasses, you may ind it useful to unfold the rubber eyeguards in order to help block out ambient room light. Leica DM500 User Manual If you have purchased a standard microscope coniguration, you will notice that the objectives are already installed on the nosepiece, and the substage condenser is already installed on the stand. In this case, go to section "Operation" on page 27. If you purchased your Leica DM500 by components and not by standard coniguration, please continue to section "Installing Objectives" on page 25. Get Ready! 24 Installing Objectives and Substage Condenser Installing objectives Always rotate the nosepiece by using the knurled nosepiece ring. • 1. As you rotate the nosepiece clockwise, attach the objectives by screwing them into the nosepiece holes starting with the lowest magniication and advancing to the highest magniication. Leica DM500 User Manual Substage condenser The Leica DM500’s substage condenser is pre-installed, pre-focused, and precentered. No additional installation or alignment is required. Get Ready! 25 Get Set! Leica DM500 User Manual Get Set! 26 Turning on the Microscope As a safety precaution, the power cord has been grounded to the microscope base. Never use an adapter between the power cord and the power source; it will render the grounding feature inefective. USB power connector The Leica DM500 has a 5 V/1.5 A USB power connector in the center of the cord wrap. This can be used to power some Leica cameras or other devices which require 5 V/1.5 A. Setting the illumination intensity Set the illumination control knob (located on the bottom left of the microscope stand) to the lowest setting to start. The illumination control knob allows you to adjust the intensity of light produced by the illumination system. • Work surface Always use your microscope on a hard, stable surface. • Power cord If the power cord is not already attached, attach it securely to the back of the microscop. • Leica DM500 User Manual Get Set! 27 Using the Condenser Plug in and turn on the microscope 1. Plug the microscope power cord into a suitable grounded electrical outlet. Using the condenser The condenser is furnished with an iris diaphragm, which can be adjusted to match the efective numerical aperture of each objective. 2. To start, fully open the iris diaphragm of the aperture condenser by rotating the condenser ring to the extreme right. A grounded 3-wire cord is provided. 2. Turn the microscope on using the switch on the bottom right-hand side of the microscope stand. 1. To open and close the iris diaphragm, simply rotate the knurled condenser ring to the right or to the left so that the line on the rotating ring aligns with the objective magniication in use. On/Off switch is located on the bottom right hand side of the stand Leica DM500 User Manual Get Set! 28 Prepare to View a Specimen Slide Prepare to view a specimen slide 1. Place a specimen slide on the stage by sliding the specimen slide forward into the slide grips. 2. Use the X/Y stage control to position the slide so a portion of the specimen is under the objective in use. Slide grips hold the slide in place. Leica DM500 User Manual Get Set! 29 Focusing Focusing 1. Rotate the nosepiece (using the knurled nosepiece ring) to move the lowest magniication objective into working position. 2. Raise the stage by rotating the coarse adjustment knob to its positive stop at the highest position. 3. Look into the eyepieces and adjust the illumination intensity so that it is comfortable for viewing. The Leica DM500 microscope stand has been factory-adjusted so that the stage focus can be found within 1.5 rotations of the ine focus adjustment from this position. 4. Using the ine adjustment knob, bring the specimen into sharp focus. Coarse adjustment knob Fine adjustment knob Leica DM500 User Manual Get Set! 30 Viewing Tube Adjustment 1. Adjust the eyetubes for your interpupillary distance. Fold or unfold the eyetubes to decrease or increase the distance between the eyepieces until you see one illuminated circle. Leica EZ viewing tube If you are using a Leica EZ viewing tube, which already has the eyepieces integrated with the eyetubes, there are no additional adjustments necessary. Be sure you are wearing your eyeglasses or contact lenses. Go on to section "Oil Immersion" on page 33. Standard tube with one or two focusing eyepieces If you are using a standard viewing tube with one or two focusing eyepieces, you need to make some adjustments: 1. Set the focusing eyepieces to the "0" diopter setting. Standard viewing tube with two fixed eyepieces If you are using a standard viewing tube with two ixed eyepieces (no focusing eyepieces), there are no additional adjustments necessary. Be sure you are wearing your eyeglasses or contact lenses. Go on to section "Oil Immersion" on page 33. The viewing tubes maintain a constant tube length for all interpupillary settings. This means that a change of interpupillary distance does not afect parfocality, magniication, or calibrations that depend on magniication. Leica DM500 User Manual Get Set! 31 Viewing Tube Adjustment (Continued) If you are comfortable wearing your corrective lenses (contact lenses or eyeglasses) for microscope viewing, leave them on and your adjustments will be minimal. 2. Focus on the specimen using the ine adjustment knob of the microscope while looking only through one of the eyepieces (if you have one focusable and one nonfocusable eyepiece, look through the nonfocusable eyepiece at this time). To help focus, cover or close the other eye. Leica DM500 User Manual 3. Now switch to look only through the other eyepiece (focusing eyepiece) with your other eye. This time, focus the specimen by using the focusing capability in the focusing eyepiece. • Do not raise or lower the stage to focus. 5. Now switch to a high magniication objective (not an oil objective) and focus the microscope while viewing with both eyes. The higher magniications have a shallower depth of ield. Therefore, after focusing with a high magniication, you will ind that when you change to lower magniications, you only have to adjust the ine focus slightly, if at all. 4. Grip the knurl on the focusing eyepiece with one hand and rotate the top of the eyepiece with the other hand until the specimen is in focus with this eye and this focusing eyepiece. This corrects for any vision diferences between your right eye and left eye. Get Set! 32 Oil Immersion Technique Oil immersion technique 1. Find the ield of view on the slide that you would like to inspect. 3. Place a drop of Leica Immersion Oil on the specimen slide on the area of the specimen that you were inspecting. 5. Raise the stage slowly using the coarse focus knob until the drop of oil on the specimen slide just comes in contact with the front of the oil immersion objective lens. 2. Lower the stage to its lowest position using the coarse focus knob. 4. Swing the oil immersion objective in place (the objective will be labeled "OIL") Leica DM500 User Manual Get Set! 6. Grip the knurled nosepiece ring and rock the objective back and forth to eliminate any air bubbles, then settle the oil objective into position with the oil between the front lens of the objective and the specimen slide. 33 Oil Immersion Technique (Continued) 7. Look through the microscope and slowly rotate the ine focus knob to raise the stage until the specimen is in focus. 8. When inished using the oil immersion objective, be sure to clean the front of the objective, the specimen slide, and any other surfaces that the oil contacted using the instructions in section "Care of the Microscope" on page 37. Leica DM500 User Manual Get Set! 34 Go! Leica DM500 User Manual Go! 35 Ready! Set! Go! Now all you need to do is change objectives, set the condenser appropriately for the objective magniication in use, and enjoy the view! Leica DM500 User Manual Go! 36 Care of the Microscope Leica DM500 User Manual Care of the Microscope 37 General Maintenance General Always carry the microscope using two hands. There is a handle on the back of the microscope and an undercut in the front for this purpose. O • The cord wrap allows you to wrap the cord in such a way that only the length you need is extended. O O O O O Leica DM500 User Manual Keep all optical components clean. Cleanliness is important for maintaining good optical performance. If any optical surface becomes coated with dust or dirt, clean the surface by blowing it of with a syringe or brushing it of with a camel hair brush before attempting to wipe the surface clean. Optical surfaces should be cleaned with a lint-free cloth, lens tissue, or cotton swab moistened with a commercially available glass cleaner. It is very important to avoid the excessive use of solvents, so use them sparingly. The lint-free cloth, lens tissue or cotton swab should be moistened with solvent, but not be wet enough for the solvent to seep around the lens. The microscope should always be covered with the plastic dust cover (provided with the instrument) when it is not in use. Care of the Microscope 38 General Maintenance (Continued) O O No part of the microscope is quite so vulnerable to collecting dirt, dust, and oil as the front lens of the objective. Whenever you encounter lack of contrast, cloudiness or poor deinition, carefully check the condition of the front lens with a magniier. Cleaning 40× and 100× objectives requires more care. Note: To achieve the high degree of latness obtained with higher magniication objectives, the objective has a small concave front lens of fairly short radius or curvature. The surface of this front lens can be readily cleaned with a toothpick covered with a cotton tip, or with a small cotton swab. Moisten the cotton with commercially available glass cleaner. Wipe the front lens lightly without applying undue force or scrubbing action. Make sure that the cotton tip contacts the concave lens surface. Check the objective with a magniier after cleaning. Leica DM500 User Manual O If you need to remove the microscope’s viewing body, be careful not to accidentally touch the outer lens surface (located on the underside of the body). Fingerprints on this surface will reduce image clarity. This lens can be cleaned in the same manner as objectives and eyepieces. Illumination O The Leica DM500 uses LED illumination. Therefore, no lamp changing is required for the life of the microscope. Care of the Microscope 39 Dimensions Leica DM500 User Manual Dimensions 40 Leica DM500 User Manual Dimensions 41 Leica DM500 User Manual Dimensions 42 Leica DM500 User Manual Dimensions 43 Leica DM500 User Manual Dimensions 44 Leica DM500 User Manual Dimensions 45 The statement by Ernst Leitz in 1907, “With the User, For the User,” describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed ive brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life. INDUSTRY DIVISION The Leica Microsystems Industry Division’s focus is to support customers’ pursuit of the highest quality end result. Leica Microsystems provide the best and most innovative imaging systems to see, measure, and analyze the microstructures in routine and research industrial applications, materials science, quality control, forensic science investigation, and educational applications. Leica Microsystems – an international company with a strong network of worldwide customer services: Active worldwide Tel. 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