THE JOURNAL
OF
BIOLOGICAL CHEMISTRY
Vol. 279, No. 30, Issue of July 23, pp. 31613–31621, 2004
Printed in U.S.A.
A Novel Fermentation/Respiration Switch Protein Regulated by
Enzyme IIAGlc in Escherichia coli*
Received for publication, May 6, 2004
Published, JBC Papers in Press, May 28, 2004, DOI 10.1074/jbc.M405048200
Byoung-Mo Koo‡, Mi-Jeong Yoon‡, Chang-Ro Lee‡, Tae-Wook Nam‡, Young-Jun Choe‡,
Howard Jaffe§, Alan Peterkofsky¶, and Yeong-Jae Seok‡储
From the ‡Laboratory of Macromolecular Interactions, School of Biological Sciences and Institute of Microbiology, Seoul
National University, Seoul 151-742, Korea, the §NINDS, National Institutes of Health, Bethesda, Maryland 20892, and
the ¶Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892
The bacterial phosphoenolpyruvate(PEP)1:sugar phosphotransferase system (PTS) plays an important role in the transport of a variety of sugar substrates. This system catalyzes
phosphorylation coupled to translocation of numerous simple
sugars across the cytoplasmic membrane. The PTS is composed
of two general cytoplasmic proteins, enzyme I (EI) and histidine phosphocarrier protein, HPr, which are used for all sugars, and, in addition, some sugar-specific components collec* This work was supported by a Korea Research Foundation Grant
(KRF-2001-015-DS0057), in part by the 21C Frontier Microbial Genomics and Applications Center Program, Ministry of Science & Technology
(Grant MG02-0201-005-2-1-0), Republic of Korea, and by BK21 Research Fellowships from the Korean Ministry of Education and Human
Resources Development (to B.-M. K., M.-J. Y., Y.-J. C., T.-W. N., and
C.-R. L.). The costs of publication of this article were defrayed in part by
the payment of page charges. This article must therefore be hereby
marked “advertisement” in accordance with 18 U.S.C. Section 1734
solely to indicate this fact.
储 To whom correspondence should be addressed. Tel.: 82-2-880-8827;
Fax: 82-2-888-4911; E-mail:
[email protected].
1
The abbreviations used are: PEP, phosphoenolpyruvate; PTS, phosphoenolpyruvate:sugar phosphotransferase system; EI, enzyme I of the
PTS; IIAGlc, the glucose-specific enzyme IIA of the PTS; HPr, histidine
phosphocarrier protein; FrsA, a novel fermentation/respiration switch
protein interacting with IIAGlc described in this work; SPR, surface
plasmon resonance; LC, liquid chromatography; MS, mass spectrometry; BSA, bovine serum albumin; PM, phenotype microarray.
This paper is available on line at http://www.jbc.org
tively known as enzymes II (1). The glucose-specific enzyme II
of Escherichia coli consists of two components: soluble enzyme
IIAGlc (IIAGlc) and membrane-bound enzyme IICBGlc (IICBGlc).
Thus, glucose transport in E. coli involves three soluble PTS
components (EI, HPr, and IIAGlc, encoded by the ptsHIcrr
operon) and one membrane-bound protein, enzyme IICBGlc (encoded by the ptsG gene). Glucose uptake entails sequential
phosphoryl transfer via the PTS, as follows: phosphoenolpyruvate (PEP) 3 EI 3 HPr 3 IIAGlc 3 IICBGlc 3 glucose.
Components of the PTS also participate in several regulatory
mechanisms (1). Catabolite repression allows for the preferential utilization of sugars transported by the PTS. Consequently,
when E. coli are cultured in a medium containing both glucose
and a non-PTS sugar, the glucose is consumed first. The currently accepted mechanism for this effect is that, when PTS
sugars are transported, the steady-state condition of IIAGlc is
mainly in the dephospho-form. The unphosphorylated form of
IIAGlc inhibits transport of non-PTS sugars such as lactose,
maltose, melibiose, and raffinose by interacting with transporters for these sugars (a process termed inducer exclusion) (1,
2– 4). Other allosteric regulatory functions of IIAGlc include
inhibition of the phosphorylation of glycerol by binding to glycerol kinase (5) and either inhibition or activation of adenylyl
cyclase (6).
E. coli IIAGlc is a protein with a Mr, predicted from its amino
acid sequence, of 18,250 and which is phosphorylated by P-HPr
at the N-3 position of His-90. In E. coli, the level of phosphorylated IIAGlc reflects not only the availability of extracellular
glucose but also the intracellular ratio of [PEP] to [pyruvate]
(7). In addition to the regulatory roles of IIAGlc listed above, we
thought that there might be regulation of other physiological
activities by IIAGlc in E. coli. This perspective stimulated us to
embark on ligand-fishing experiments aimed at detecting other
soluble protein(s) exhibiting high affinity binding to IIAGlc. In
our previous study, employing ligand-fishing in E. coli extracts
with surface plasmon resonance spectroscopy and immobilized
HPr as the bait, we discovered a high affinity binding of glycogen phosphorylase to HPr; thus, HPr mediates cross-talk between sugar uptake through the PTS and glycogen breakdown
(8 –10). In this study, ligand-fishing, using surface plasmon
resonance spectroscopy, was also employed to search for a high
affinity binding of IIAGlc to a protein in E. coli extracts. Consequently, we discovered a new IIAGlc-binding protein in E. coli
extracts. The protein was purified and established to be the
previously uncharacterized yafA gene product, which we now
refer to as a fermentation/respiration switch protein (FrsA).
This report also describes experiments aimed at deducing the
locus of action of FrsA.
31613
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The bacterial phosphoenolpyruvate:sugar phosphotransferase system regulates a variety of physiological
processes as well as effecting sugar transport. The crr
gene product (enzyme IIAGlc (IIAGlc)) mediates some of
these regulatory phenomena. In this report, we characterize a novel IIAGlc-binding protein from Escherichia
coli extracts, discovered using ligand-fishing with surface plasmon resonance spectroscopy. This protein,
which we named FrsA (fermentation/respiration switch
protein), is the 47-kDa product of the yafA gene, previously denoted as “function unknown.” FrsA forms a 1:1
complex specifically with the unphosphorylated form
of IIAGlc, with the highest affinity of any protein thus
far shown to interact with IIAGlc. Orthologs of FrsA
have been found to exist only in facultative anaerobes
belonging to the ␥-proteobacterial group. Disruption
of frsA increased cellular respiration on several sugars
including glucose, while increased FrsA expression resulted in an increased fermentation rate on these sugars with the concomitant accumulation of mixed-acid
fermentation products. These results suggest that IIAGlc
regulates the flux between respiration and fermentation
pathways by sensing the available sugar species via a
phosphorylation state-dependent interaction with FrsA.
Interaction of Enzyme IIAGlc with FrsA
31614
TABLE I
Bacterial strains and plasmids used
Strains and plasmids
Strains
MG1655
DY330
GI698
GI698⌬pts
SR702⌬ptsG
TP2811
BM100
BM101
BM201
Plasmids
pRE1
pR3
pKY103
pKY103H
pBR322
pKY513
Relevant genotype or phenotype
Source or Ref.
Wild type E. coli
W3110 ⌬lacU169 gal490 CI857 ⌬(cro-bioA)
F⫺ ⫺ lacIq lacPL8 ampC::Ptrp cI
GI698 ⌬(ptsH, ptsI, crr), Kmr
araD139 ⌬argF-lacU169rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR suhX1
⌬ptsG::cat Cmr
F⫺, xyl, argH1, ⌬lacX74, aroB, ilvA, ⌬(ptsH, ptsI, crr), Kmr
DY330 ⌬frsA::cat, Cmr
MG1655 ⌬frsA::cat, Cmr
GI698 ⌬frsA::cat, Cmr
32
17
33
34
Gift from S. R. Ryu
Expression vector under control of PL promoter, Ampr
crr (NdeI/BamHI) in pRE1, Ampr, overexpression vector for IIAGlc
frsA (NdeI/BamHI) in pRE1, Ampr, overexpression vector for FrsA
frsA (NdeI/BamHI) with N-terminal 6 hisditines in pRE1, Ampr, overexpression
vector for His-FrsA
11
16
This work
This work
frsA in pBR322, Ampr
This work
EXPERIMENTAL PROCEDURES
FrsA were grown in 200 ml of M9 salts-based rich medium to an A600 of
1.5, harvested, and washed with 30 ml of 20 mM HEPES buffer, pH 8.0,
containing 100 mM NaCl. The cell pellets were resuspended in 3 ml of
the buffer in the presence of 500 M phenylmethylsulfonyl fluoride. The
cell suspension was disrupted in a French pressure cell and centrifuged
at 100,000 ⫻ g for 30 min at 4 °C, and the supernatant was used as the
crude extract. The crude extract was incubated with 200 l of BD
TALONTM resin. After the mixture was loaded onto a Poly-Prep chromatography column (8 ⫻ 40 mm) (Bio-Rad), the column was washed
twice with the wash buffer (20 mM HEPES, pH 7.0, with 300 mM NaCl)
containing 10 mM imidazole. The proteins bound to the column were
eluted with the wash buffer containing 200 mM imidazole and analyzed
by SDS-PAGE followed by staining with Coomassie Blue and Western
blotting using anti-IIAGlc serum raised in mice.
Identification of Coomassie Blue-stained Bands—Coomassie Bluestained gel bands were excised and subjected to in-gel proteolytic digestion with trypsin according to the method of Moritz et al. (13).
Digests were subjected to LC-MS analysis on a previously described (14)
Michrom Bioresources Magic 2002 model microbore high performance
liquid chromatography coupled to a model LCQ ion trap mass spectrometer (Finnigan) equipped with an electrospray interface utilizing a 0.3 ⫻
150 mm Magic MS C18 column (Michrom Bioresources) eluted at 8
l/min and 40 °C with a linear gradient of 2– 65% Solvent B over 30
min. Solvent A was 10/10/980/1/0.005 CH3CN/1-PrOH/H2O/acetic acid/
hexafluorobutyric acid (v/v/v/v/v) and Solvent B was 700/200/100/0.9/
0.005 CH3CN/1-PrOH/H2O/acetic acid/hexafluorobutyric acid (v/v/v/v/
v). Column effluent was monitored at 215 nm. The mass spectrometer
was operated in the “Top Five” mode in which the instrument was set
up to automatically acquire (a) a full scan between m/z 300 and m/z
1300 and (b) tandem MS/MS spectra (relative collision energy ⫽ 35%) of
the five most intense ions in the full scan. MS/MS spectra were analyzed using the Bioworks software package (Finnigan). Individual uninterpreted MS/MS spectra were searched against the non-redundant
data base utilizing the SEQUEST program (by J. Eng and J. Yates,
University of Washington, Department of Molecular Biotechnology,
Seattle, WA). Search parameters were set to a static modification of
⫹71 atomic mass units to allow for acrylamide alkylation of cysteine
and enzyme specificity was set to “no enzyme.” Fragment ions were
labeled using the Bioworks software.
Purification of Overexpressed Proteins—The steps of purification
were followed by SDS-PAGE. Protein concentration was determined by
the bicinchoninic acid protein assay (Pierce). E. coli GI698 harboring
pKY103 was used for overexpression of FrsA. Cell culture and induction
of protein overexpression was done as described previously (15). The
cell pellet obtained from 500 ml of culture containing overexpressed
FrsA was resuspended in buffer A (10 mM Tris䡠HCl, pH 7.5, containing
50 mM NaCl) and then passed three times through a French pressure
cell at 10,000 p.s.i. The lysate was cleared of cell debris by centrifugation at 100,000 ⫻ g for 90 min. The soluble fraction was chromatographed through a DEAE-Sepharose (Sigma) column (2.5 ⫻ 10 cm)
using a gradient of 50 –500 mM NaCl (220 ml). The fractions containing
substantial amounts of FrsA were pooled and concentrated in a 3 K
Macrosep centrifugal concentrator (Pall Gelman Laboratory, Ann
Arbor, MI). The concentrated pool was further purified on a hydroxy-
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Strains, Plasmids, and Growth—The bacterial strains and plasmids
used in this study are listed in Table I. Luria-Bertani (LB) medium was
used for routine bacterial growth except with strain GI698 and its
derivatives. M9 salts-based rich medium (supplemented with 2%
casamino acids and 1% glycerol) was used for routine culture of GI698
and its derivatives. DY330, GI698, and their derivatives were grown at
30 °C, while other strains listed in Table I were grown at 37 °C. For the
overproduction of proteins using GI698 and the pRE1-based vector
system, cells were grown in M9 salts-based induction medium (0.2%
casamino acids, 1% glycerol, and M9 salts) at 30 °C. Tryptophan (final
concentration: 100 g/ml) was added to the culture medium when the
culture reached an A600 ⫽ 0.4, and cells were harvested 18 –20 h after
induction. Antibiotics were used at the following concentrations: ampicillin, 100 g/ml; kanamycin, 20 g/ml; chloramphenicol, 30 g/ml.
To construct pKY103, the DNA sequence from nucleotide 3227 to
4541 of the E. coli frsA gene (GI:2367098) was amplified by PCR, using
mutagenic primers to create an NdeI site (underlined) at the ATG start
codon (5⬘-TCTGGAGGCTGCACATATGCACAGGCAAA-3⬘) and a
BamHI site (underlined) 39 nucleotides downstream from the TAA stop
codon (5⬘-TATCTCCTGTTGGGATCCAACTGTTTTACC-3⬘). The internal NdeI site located at codons 186 and 187 was removed by mutagenesis (CATATG to CACATG) so that this did not result in any amino acid
changes. The PCR product, digested with NdeI and BamHI, was cloned
into the vector pRE1 (11), resulting in the recombinant plasmid pKY103
for FrsA overproduction.
To construct pKY513, in which FrsA expression is under the control
of its own promoter, the sequence covering its own promoter and coding
regions was amplified by PCR using mutagenic primers to create an
EagI site (underlined) 432 nucleotides upstream of the frsA start codon
(5⬘-TGCCGTAAGCCGTGGCGGCCGGGTACCGGG-3⬘) and a BamHI
site (underlined) 39 nucleotides downstream from the TAA stop codon
(5⬘-TATCTCCTGTTGGGATCCAACTGTTTTACC-3⬘). The PCR product, digested with EagI and BamHI, was cloned into the vector pBR322.
Measurement of Protein-Protein Interaction—The interaction of IIAGlc
and its binding factor was monitored by surface plasmon resonance
(SPR) detection using a BIAcore 3000 (BIAcore AB) as described previously (8, 12). IIAGlc and IIBGlc were separately immobilized onto the
carboxymethylated dextran surface of a CM5 sensor chip. IIAGlc and
IIBGlc (80 l, 100 g/ml) in coupling buffer (10 mM sodium acetate, pH
4.0) were allowed to flow over a sensor chip at 10 l/min to couple the
proteins to the matrix by a N-hydroxysuccinimide/N-ethyl-N⬘(3-diethylaminopropyl)-carbodiimide reaction (80 l of mix). Unreacted N-hydroxysuccinimide was inactivated by injecting 80 l of 1 M ethanolamine HCl, pH 8.0. Assuming that 1000 resonance units correspond to
a surface concentration of 1 ng/mm2, the proteins IIAGlc and IIBGlc were
immobilized to a surface concentration of 1.5 and 1.8 ng/mm2, respectively. The standard running buffer was 10 mM HEPES, pH 7.4, 150 mM
NaCl, 10 mM KCl, 1 mM MgCl2, and 1 mM dithiothreitol, and all
reagents were introduced at a flow rate of 10 l/min. The sensor surface
was regenerated between assays by injecting 10 l of 2 M NaCl to
remove bound analyte.
To examine the in vivo interaction between IIAGlc and FrsA, GI698
cells harboring pKY103 expressing FrsA or pKY103H expressing His-
19
This work
This work
This work
Interaction of Enzyme IIAGlc with FrsA
RESULTS
Immobilized IIAGlc Complexes with a Factor in E. coli Extracts—The technique of ligand-fishing by SPR (8) was used to
search for a protein that might interact with and be regulated
by IIAGlc. SPR detects a change in refractive index resulting
from the interaction of a soluble protein with another protein
covalently linked to a surface. IIAGlc, immobilized on a CM-5
sensor chip (see “Experimental Procedures”), was used as the
bait. To minimize nonspecific interactions between the crude
extract and IIAGlc, a cytoplasmic crude extract from a stationary phase culture of an E. coli strain (TP2811) carrying a
deletion of the pts operon (see “Experimental Procedures”) (19)
was chromatographed through a Mono Q 10/10 column (Amersham Biosciences) using a linear gradient of 50 –500 mM NaCl
(80 ml total volume). Each fraction was allowed to flow over
the immobilized IIAGlc for 10 min; we found that fractions
eluting near 100 mM NaCl showed a detectable increase in SPR
response (Fig. 1A).
Since IIAGlc interacts with glycerol kinase, it seemed possible that the detected interaction might be due to formation of
the glycerol kinase-IIAGlc complex. However, when purified
glycerol kinase (120 l of 10 g/ml protein) was allowed to flow
over the immobilized IIAGlc in the absence of Zn2⫹ (20) for 10
min, no interaction was detectable (data not shown). Furthermore, because the ptsHIcrr operon is deleted in strain TP2811
(19), the possibility of interaction of HPr and IIAGlc was also
excluded. This result prompted us to purify this new IIAGlcbinding protein.
Purification of the Factor Interacting with IIAGlc—The approach to purification of the factor that interacts with IIAGlc
was based on SPR analysis; fractions from each purification
step were examined for binding to immobilized IIAGlc using the
BIAcore (Fig. 1). E. coli strain TP2811 was grown to stationary
phase in 6 liters of Luria broth at 37 °C. The harvested cell
pellet was washed and resuspended in buffer A (10 mM
Tris䡠HCl, pH 7.5, containing 50 mM NaCl); this suspension was
passed three times through a French pressure cell at 10,000
p.s.i. The lysate was centrifuged at 100,000 ⫻ g for 90 min to
obtain the soluble cytoplasmic fraction. This fraction was chromatographed through a DEAE-Sepharose (Sigma) column (8 ⫻
30 cm) using a linear gradient of 50 –500 mM NaCl (2 liters total
volume). Fractions eluting around 100 mM NaCl demonstrated
interaction with immobilized IIAGlc; these fractions were
pooled and concentrated in a 3 K Macrosep centrifugal concentrator (Pall Gelman Laboratory). The concentrated pool was
further purified on a hydroxyapatite (Bio-Rad) column (1.5 ⫻
10 cm) using a linear gradient of 0 –500 mM potassium phosphate buffer, pH 7.5 (80 ml total volume). Fractions eluting
around 100 mM potassium phosphate demonstrated interaction
with immobilized IIAGlc, and these fractions were pooled and
concentrated as described above. The concentrated pool was
chromatographed on a Superdex 75 column (Amersham Biosciences) equilibrated with buffer A. Once again, the eluted
fractions were analyzed using SPR, and fractions showing affinity toward immobilized IIAGlc were pooled and concentrated
as described for previous steps. As a final purification step, a
Mono Q 5/5 column (Amersham Biosciences) was used with a
15-volume NaCl gradient (50 –300 mM) in buffer A. The fractions eluting near 100 mM NaCl, showing interaction with
IIAGlc, were pooled, and the protein was precipitated by adding
acetone to a final concentration of 80% after BSA (1 mg/ml) was
added to assist in recovery.
The IIAGlc-binding Protein Is the yafA Gene Product—The precipitated proteins were solubilized in SDS-loading buffer and
subjected to SDS-PAGE. Staining of the gel with Coomassie Blue
revealed a protein band migrating with an apparent molecular
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apatite (Bio-Rad) column (1.5 ⫻ 10 cm) using a gradient of 0 –500 mM
potassium phosphate buffer, pH 7.5 (80 ml). Fractions containing FrsA
were pooled and concentrated. The concentrated fraction was chromatographed on a HiLoad 16/60 Superdex 75 prepgrade column (Amersham
Biosciences) equilibrated with buffer A. Finally, a Mono Q 5/5 (Amersham Biosciences) column was used with 15 volumes of NaCl gradient
(50 –300 mM) to obtain homogeneous FrsA (⬎98% pure). The yield of
pure FrsA from 500 ml of culture was ⬃2 mg. EI, HPr, IIAGlc, and
EIIBGlc were purified as described previously (12, 15, 16).
Gel Filtration Chromatography of IIAGlc-FrsA Complex—Gel filtration chromatography was performed in a AKTA-FPLC system (Amersham Biosciences). One-ml samples containing either 4 mg of IIAGlc or
4 mg of FrsA or both proteins in 20 mM Tris-HCl, pH 8.0, containing 50
mM NaCl were incubated for 10 min on ice and injected through a
Superose 12 column (25 ⫻ 500 mm; Amersham Biosciences) equilibrated with the same buffer. Filtration was performed at room temperature at a flow rate of 1 ml/min. Fractions of 3 ml were collected.
Native Polyacrylamide Gel Electrophoresis—Mobility shifts of FrsA
due to its interaction with IIAGlc were demonstrated in a nondenaturing
4 –20% gradient gel (Novex). Tris䡠glycine, pH 8.3, was used as the
running buffer. A binding mixture (20 l) of 100 mM Tris䡠HCl, pH 7.5,
2 mM MgCl2, 10 mM KCl, 1 mM dithiothreitol, 200 g/ml FrsA, and 50
g/ml IIAGlc was allowed to incubate at room temperature for 30 min.
This mixture was combined with 5 l of 5⫻ loading buffer (0.01%
bromphenol blue, 0.5 M Tris䡠HCl, pH 6.8, 50% glycerol) and electrophoresed in the gel for 2 h at 100 V. After electrophoresis, the gel was
stained with Coomassie Brilliant Blue.
Measurement of Glucose Concentration—Glucose concentrations
were measured using a glucose assay kit (Sigma) following the supplier’s instruction with some modifications as described previously (9).
Cells were withdrawn every 2 h and centrifuged at 10,000 ⫻ g for 5 min.
Supernatant solutions were used to determine glucose remaining in the
culture medium. To determine glucose in the medium, each aliquot of
culture broth was diluted 100-fold in water, and 20 l of diluted solution
was mixed with 200 l of combined enzyme-color reagent solution and
incubated at 37 °C for 30 min. After color development, absorbance was
measured at 450 nm. To prepare the combined enzyme-color reagent
solution, 200 mg of enzyme mixture containing 100 units of Aspergillus
niger glucose oxidase, 20 Purpurogalin units of horseradish peroxidase,
and buffer salts was dissolved in 20 ml of distilled water, and mixed
with 320 l O-dianisidine dihydrochloride solution (2.5 mg/ml in distilled water).
Disruption of frsA—The frsA gene was disrupted using electroporated linear DNA amplified by PCR and E. coli DY330 as described
previously (17). The frsA gene (nucleotides 7–1164 from the start codon)
was replaced by a chloramphenicol acetyltransferase gene (cat). cat was
amplified from SR702 (ptsG::cat) with the following primers; forward
primer, 5⬘-GCGGGTTACA ATAGTTTCCA GTAAGTATTC TGGAGGCTGC ATCCATGACA GAGAAAAAAA TCACTGGATA-3⬘ and reverse
primer, 5⬘-GATTTCCTGA AGACCTTTGT CAAAATTCCG ATACACCGGG TTAAATGGGA CGCCCCGCCC TGCCACTCAT-3⬘.
MG1655 ⌬frsA (BM101) and GI698 ⌬frsA (BM201) were constructed
by P1 transduction of the Cmr region of BM100. Disruption of the frsA
gene was confirmed by PCR.
Analysis of Metabolic Products by NMR Spectroscopy and Measurement of Acetate Concentration—Cells were grown in 200 ml of M9 salts
medium containing 0.5% glucose or other indicated sugars and 0.2%
casamino acids supplemented with 1 mM MgCl2, 0.1 mM CaCl2 and 100
g/ml ampicillin. Aliquots of culture were taken at the indicated time
and were centrifuged at 3000 ⫻ g for 10 min at 4 °C. Supernatant
solutions were used to analyze the extracellular metabolic products.
The cell-free culture solutions (0.9 ml each) taken at 12 h after inoculation were mixed with D2O (0.1 ml) and subjected to the NMR experiments. The spectra were recorded at a 1H resonance frequency of 500
MHz by use of a Bruker DRX500 spectrometer.
The concentration of acetate in culture supernatants was measured
as described previously (18) with slight modifications. The assay mixture contained in a final volume of 3 ml (final concentration in parentheses): potassium phosphate, pH 7.6 (150 mM), MgCl2 (20 mM), PEP
(0.5 mM), NADH (0.5 mM), ATP (5 mM), pyruvate kinase/lactate dehydrogenase (4.4 and 6.0 units/ml, respectively), and samples or 10 –150
M sodium acetate to make a standard curve. The reagents were mixed
and allowed to equilibrate for 10 min at 37 °C. After noting the absorbance at 365 nm (A1), 20 l of acetate kinase was added to a final
concentration of 1.5 units/ml. The reagents were mixed again and
incubated at 37 °C for 60 min before noting the final absorbance at 365
nm (A2). The change in absorbance (⌬A ⫽ A1 ⫺ A2) was calculated and
related to concentration by reading from a standard plot.
31615
31616
Interaction of Enzyme IIAGlc with FrsA
mass of ⬃47 kDa in addition to a band corresponding to BSA. The
bands in the vicinity of the 47-kDa protein were cut out of the gel
and subjected to in-gel proteolytic digestion. Digests were analyzed by LC/MS/MS (see “Experimental Procedures”) to identify
proteins. Protein in the slower migrating (presumptive BSA)
band was identified as BSA on the basis of the following tryptic
peptides: (K)SEIAHR, (K)DLGEEHFK, (R)FKDLGEEHFK,
(K)HLVDEPQNLIK, (R)KVPQVSTPTLVEVSR, and (K)TSESGELHGLTTEDK. Protein in the faster migrating band was identified as a mixture of BSA on the basis of the following tryptic
peptides: (K)HLVDEPQNLIK, (R)KVPQVSTPTLVEVSR, (R)RPCFSALTPDETYVPK, (K)LVNELTEFAK, (R)RHPEYAVAVLLR, (K)LGEYGFQNAILVR, and (K)TSESGELHGTLTTEDK
and YAFA_ECOLI (P04335) on the basis of the following tryptic
peptides: (R)LGMHDASDEALR, (K)GDDLAEQAQALSNR,
(R)VAAFGFR, (R)TDDDLYDTVIGYR, and (M)TQANLSETLFKPR (see Fig. 1B).
On the basis of the sequence analyses, we concluded that the
BSA sequence was a carryover from the added protein (see
above) and that the YAFA_ECOLI sequence was likely to correspond to the IIAGlc-binding factor. The correspondence of the
apparent molecular mass by SDS-PAGE of the IIAGlc-binding
protein with the calculated molecular mass of YAFA_ECOLI
(47,008 Da) was consistent with this prediction.
Overexpression and Purification of the frsA Gene Product—To further explore the possibility that the IIAGlc-binding
protein corresponded to the product (YAFA_ECOLI) of the yafA
gene (we refer to yafA as frsA, since we report here that its gene
product (FrsA) acts as a fermentation/respiration switch), the
gene was cloned into an expression vector so that sizeable
amounts of the pure protein could be produced. For overexpression of FrsA, we constructed the pRE1-based recombinant plasmid, pKY103. In this plasmid, genes are under the control of
the strong PL promoter-cII ribosome binding site combination
(11, 15). E. coli GI698, transformed with the pKY103 recombinant plasmid, was used for overexpression of the gene product.
This strain was grown as described under “Experimental Procedures,” and the overexpressed protein with molecular mass
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FIG. 1. Purification of the factor interacting with IIAGlc. A, SPR analysis of fractions showing interaction with immobilized IIAGlc. E. coli
TP2811 cell extract was fractionated by Mono Q 10/10 chromatography. An aliquot of each fraction was allowed to flow over the IIAGlc surface to
test for binding as described under “Experimental Procedures.” Solid bars indicate the signal from the IIAGlc immobilized surface. The solid line
shows the salt gradient. Fraction 13 corresponds to the portion of the salt gradient containing about 100 mM NaCl. B, the scheme of purification
and identification of IIAGlc-binding protein. Full-scale MS/MS spectrum of the doubly charged ion was obtained at m/z 753.3. The spectrum was
analyzed by the SEQUEST program to give the N-terminal tryptic peptide TQANLSETLFKPR. Observed y and b ions, including a continuous
y2–y11 series and b2, b4 – b7, b10, and b11 ions, consistent with those predicted for the peptide, are labeled.
Interaction of Enzyme IIAGlc with FrsA
about 47 kDa corresponded to about 30% of the total cell protein after induction. After sequential purification steps, as described under “Experimental Procedures,” ⬃98% pure protein
was obtained as judged by SDS-PAGE (data not shown). The
purified protein bound to immobilized IIAGlc with high affinity,
indicating that the product of the frsA gene is the IIAGlcbinding protein. The dissociation constant (KD) for the interaction between IIAGlc and its binding protein, using BIAevaluation 2.1 software, was determined to be ⬃1.8 ⫻ 10⫺7 M,
assuming interaction of the monomeric forms of both IIAGlc and
its binding protein (data not shown). From the comparison of
SPR response units of the IIAGlc-binding protein toward immobilized IIAGlc in Fig. 1 with that of the purified recombinant
FrsA, the cellular concentration of the IIAGlc-binding protein
was calculated to be about 0.27 M in wild type cells.
FrsA Binds Specifically to Unphosphorylated IIAGlc to Form
a Heterodimer—Generally, the regulatory functions of the PTS
depend on the phosphorylation state of the involved protein (1).
Accordingly, the effect of phosphorylation of IIAGlc on the affinity for its binding protein was checked by SPR and native gel
electrophoresis (Fig. 2). To phosphorylate immobilized IIAGlc,
PEP, EI, and HPr were mixed and incubated for 20 min, and
then this mixture was allowed to flow over the immobilized
IIAGlc surface. When IIAGlc was phosphorylated, binding of the
IIAGlc-binding factor to the immobilized IIAGlc surface was
almost completely abolished (Fig. 2A, sensorgram b) while
there was significant binding to the unphosphorylated form of
IIAGlc (Fig. 2A, sensorgram a). The specificity of the binding
was supported by the observation that FrsA did not bind to
immobilized cloned IIBGlc (12). When EI, HPr, or the soluble
IIBGlc domain (4 g/ml, respectively) was allowed to flow over
a surface immobilized with FrsA, no signal was detected (data
not shown).
The biomolecular interaction studies by SPR indicated a high
affinity interaction of IIAGlc and its binding protein. It was
therefore anticipated that such a stable complex might survive
under electrophoretic conditions. Consequently, gel mobility
shift experiments using nondenaturing polyacrylamide gel
electrophoresis were carried out (Fig. 2B). The band corresponding to FrsA smeared just below the loading wells (lanes
3– 6 in Fig. 2B) unless IIAGlc was co-loaded (lane 7). While FrsA
significantly retarded the mobility of IIAGlc in the gel (lane 7 in
Fig. 2B), it did not lead to a mobility shift of EI, HPr, or IIBGlc,
consistent with the SPR data. When FrsA was run with IIAGlc
under phosphorylating conditions (incubating the reaction mixture in the presence of EI, HPr, and PEP before loading), the
band corresponding to the complex between IIAGlc and FrsA
was hardly visible (compare lanes 7 and 8 in Fig. 2B). These
data confirm the previous observation that FrsA interacts specifically with IIAGlc and that this interaction depends on the
phosphorylation state of IIAGlc.
The tight interaction between IIAGlc and FrsA was also confirmed by gel filtration analysis. The elution profile from a
Superose 12 gel filtration column (25 ⫻ 500 mm) of the complex
was compared with those of individual proteins. FrsA alone
eluted as a symmetrical peak at 59 ml, corresponding to the
monomeric form (47 kDa), while isolated IIAGlc eluted at 61 ml
(Fig. 3A). When IIAGlc and FrsA (4 mg each) were mixed in a
buffer (20 mM Tris䡠HCl buffer, pH 7.5, with 50 mM NaCl) and
loaded onto the column, a unique peak eluted at 55 ml (Fig. 3A).
When fractions (18 –20) corresponding to this peak were run on
a SDS-PAGE gel, they resolved into two bands migrating at the
positions expected for IIAGlc and its binding protein (Fig. 3B).
Fraction 21 corresponds to unbound IIAGlc. When the fractions
(19 and 20) corresponding to the complex peak were reloaded
onto the same column, they eluted again as a single peak at the
same elution volume, suggesting that the complex was stable
even after its dilution resulting from the two successive gel
filtration chromatography runs (data not shown). Based on the
elution profile of the complex through the gel filtration column
and the band intensity on SDS-PAGE of the corresponding
fractions, it appears that IIAGlc interacts with FrsA in a oneto-one ratio to form a heterodimer.
Evidence for the in Vivo Interaction between IIAGlc and
FrsA—The in vitro experiments described above demonstrated
a phosphorylation state-dependent high affinity interaction of
IIAGlc with FrsA. To examine whether formation of the direct
complex occurs in vivo, FrsA with N-terminal 6 histidine residues (His-FrsA) was expressed in cells lacking the chromosomal frsA gene. The crude extract prepared from these cells
was subjected to a pull-down assay using BD TALONTM resin
(Clontech). As shown in Fig. 4, IIAGlc, but not its phospho-form,
was co-eluted with His-FrsA bound to the beads, although
about 50% of total IIAGlc existed in its phosphorylated form as
judged from Western blot analysis, while no IIAGlc was recovered from the bound fraction when the crude extract was prepared from cells expressing unmodified FrsA. These results
provide confirmation that the phosphorylation state-dependent
interaction between FrsA and IIAGlc also occurs in vivo.
Phenotype Microarray Analysis Indicates That Deletion of
frsA Increases Cell Respiration Rate on Several Sugars—To
assay the effects of frsA disruption on cell respiration, phenotype analysis was carried out using phenotype microarrays
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FIG. 2. Specific interaction between FrsA and unphosphorylated IIAGlc. A, interaction of purified FrsA with immobilized unphosphorylated or phosphorylated IIAGlc. SPR analysis was carried out as
described under “Experimental Procedures.” Phosphorylated IIAGlc was
formed by passing a solution containing PEP, EI, and HPr over a
surface of immobilized IIAGlc. Significant interaction signal was obtained when FrsA was allowed to flow over the dephospho-form of IIAGlc
(sensorgram a), but not with the phosphorylated form of IIAGlc (sensorgram b). The arrows indicate the starting points of injections. B, electrophoretic mobility shift of IIAGlc complexed with FrsA. The specificity
of interaction between FrsA and the unphosphorylated form of IIAGlc
was tested on a nondenaturing PAGE gel. Lane 1, EI; lane 2, HPr; lane
3, FrsA; lane 4, EI ⫹ FrsA; lane 5, HPr ⫹ FrsA; lane 6, IIBGlc ⫹ FrsA;
lane 7, IIAGlc ⫹ FrsA; lane 8, EI ⫹ HPr ⫹ IIAGlc ⫹ FrsA ⫹ PEP
(phosphorylated form of IIAGlc); lane 9, IIAGlc; lane 10, IIBGlc.
31617
31618
Interaction of Enzyme IIAGlc with FrsA
FIG. 3. Gel filtration chromatography of IIAGlc, FrsA, and the
IIAGlc-FrsA complex. Samples were preincubated and injected on a
Superose 12 column as described under “Experimental Procedures.” A,
the three chromatograms were obtained separately and two were superimposed to compare the elution profiles. The dashed line corresponds to injection of FrsA alone (4 mg), and the solid line corresponds
to the mixture of IIAGlc and FrsA (4 mg each). The chromatogram for
IIAGlc was not included because it showed little absorbance at 280 nm.
Arrows indicate elution volumes of molecular weight markers used to
calibrate the column: arrow 1, RNase (14.7 kDa); arrow 2, chymotrypsinogen (20.2 kDa); arrow 3, ovalbumin (47.2 kDa); and arrow 4,
albumin (61.6 kDa). B, Coomassie Brilliant Blue-stained SDS-PAGE
gel loaded with gel filtration chromatography fractions (3 ml for each
fraction) of the IIAGlc-FrsA mixtures. Lane M indicates Mark 12TM
protein molecular mass standards (Invitrogen), and numbered lanes
indicate fractions from gel filtration chromatography of the mixture.
Molecular masses (in kDa) of some standards are presented on the
left side.
(PMs) (Biolog, Inc.). This technique involves the deduction of
cellular phenotypes using cell respiration as the reporter system. The assay chemistry uses a tetrazolium dye to colorimetrically detect the respiration of cells. Reduction of this dye due to
cell respiration during growth results in formation of a purple
color, and it accumulates in the well over the incubation period;
the data (see Fig. 5) are presented as a plot of the respiration
over time (21, 22). Therefore, the more respiratory activity
growing cells have, the more purple color should accumulate.
Total loss of function will result in no growth and no purple
color. Therefore, the colorimetric assay of respiration can provide a virtually universal reporter system for phenomic testing.
PM tests were performed on BM101, compared with the control
strain MG1655, in a set of 20 96-well microplates containing
FIG. 5. Phenotype microarray comparison of MG1655 and
BM101. Significant changes (more than 60% difference compared with
MG1655) are enclosed in boxes and indicated by arrows. Yellow, red,
and green colors indicate similar growth of the wild type and mutant,
faster growth of the wild type, and faster growth of the mutant, respectively. Growth was analyzed by Biolog, Inc. Detailed information of PM
experiments is available at www.biolog.com.
different nutrients or inhibitors; this allowed measurement of
cell respiration to examine nearly 2000 cellular phenotypes in
a sensitive, highly controlled, reproducible format (further extensive information concerning PM technology can be obtained
at www.biolog.com). The results indicated that BM101 showed
faster purple color development using carbon sources such as
fructose, arabinose, glucose, and lactose than its parental
strain (MG1655), while there was no significant change in
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FIG. 4. In vivo interaction between dephospho-IIAGlc and FrsA.
GI698⌬frsA cells harboring pKY103 expressing FrsA or pKY103H expressing His-FrsA were grown as described under “Experimental Procedures.” The cell suspension disrupted in a French pressure cell was
centrifuged, and the supernatant was mixed with BD TALONTM resin.
The mixture was loaded onto a column (Bio-Rad) and the proteins
bound to the resin were eluted with 200 mM imidazole and analyzed by
SDS-PAGE followed by staining with Coomassie Brilliant Blue (A) and
Western blotting using anti-IIAGlc serum rasied in a mouse (B). Lane 1,
crude extract of GI698⌬frsA/pKY103; lane 2, crude extract of
GI698⌬frsA/pKY103H; lane 3, eluate of crude extract of GI698⌬frsA/
pKY103 from the resin; and lane 4, eluate of crude extract of
GI698⌬frsA/pKY103H from the resin. Molecular masses (in kDa) of
some standards are presented on the left side.
Interaction of Enzyme IIAGlc with FrsA
DISCUSSION
Each protein component of the E. coli PTS involved in glucose uptake has been shown to regulate the activity of a partner
protein by a direct protein-protein interaction. These regula-
FIG. 6. Growth rate-dependent glucose consumption of
MG1655 and its derivatives. Growth rates (A) and the amount of
glucose remaining in the medium (B) are recorded as a function of
incubation time. Cells were grown aerobically with shaking at 200 rpm
in M9 minimal medium supplemented with 0.2% casamino acids and
0.5% glucose at 37 °C. This experiment was repeated several times and
the growth profiles were reproducible. Representative data are shown
here. Squares, MG1655/pBR322; circles, BM101/pBR322; triangles,
BM101/pKY513.
tory functions of the PTS depend on the phosphorylation state
of the involved component; the phosphorylation state of PTS
proteins has been shown to increase in the absence and decrease in the presence of a PTS sugar substrate. The ratio of
phosphorylated to dephosphorylated proteins in turn serves as
signal input for the control of these physiological processes.
Unphosphorylated EI interacts with and regulates the autophosphorylation activity of CheA to trigger chemotaxis toward
PTS carbohydrates (23). Unphosphorylated IIAGlc inhibits glycerol kinase and several sugar permeases by a mechanism
termed inducer exclusion (1, 5). Phosphorylated IIAGlc stimulates cAMP synthesis (6). Unphosphorylated HPr interacts
with and stimulates the activity of glycogen phosphorylase in
vitro (8, 10) and in vivo (9). More recently, it was also discovered that the membrane-bound glucose transporter enzyme
IICBGlc interacts with Mlc to modulate the cellular localization
and activity of the global repressor protein Mlc in a phosphorylation state-dependent manner (12, 24, 25).
We have been interested in searching for other physiological
activities exerted by the PTS in E. coli. Consequently, we
performed ligand-fishing experiments aimed at detecting soluble protein(s) exhibiting binding to IIAGlc with a higher affinity
than to glycerol kinase (26). The same methodology, employing
SPR, as used before to demonstrate the high affinity interaction of HPr with glycogen phosphorylase (8), was followed. Our
preliminary data showed that the interaction between glycerol
kinase and IIAGlc was detectable by SPR only in the presence of
Zn2⫹ (data not shown). This result is supported by the previous
report that Zn2⫹ promoted the association between glycerol
kinase and IIAGlc (20). Furthermore, it is worth noting that no
interaction was detectable when purified EI was exposed to
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other phenotypes. From these studies, it was apparent that, out
of about 2000 tests, an increase in respiration rate on several
sugars is the only phenotype detected due to the deletion of frsA
(Fig. 5).
To check whether episomal frsA expression can complement
the deletion phenotype, an expression plasmid (pKY513) in
which frsA expression is under the control of its own promoter
was constructed and transformed into BM101. To construct
pKY513, the sequence from 432 bp upstream of the frsA start
codon to 39 bp downstream of the stop codon was amplified by
PCR and cloned into pBR322. The expression level of FrsA from
this plasmid was determined to be about 5-fold greater than
wild type but still much less than that of IIAGlc by Western blot
analysis (data not shown). To confirm the phenotype microarray data, we carried out phenotype tests of MG1655, BM101,
and BM101/pKY513 using GN2 microplates (Biolog, Inc.) containing 96 different carbon sources. Although carbon catabolic
activities of the three tested strains showed no significant
differences, the rate of color development was different from
strain to strain. BM101 showed faster purple color development using the carbon sources fructose, arabinose, glucose,
lactose, and mannitol as expected from the PM test described
above. It is worth noting that introduction of pKY513 into the
BM101 strain resulted in the same phenotype as the wild type
(MG1655), indicating that the increased respiration rate of
BM101 on several sugars was correlated with deletion of the
frsA gene from MG1655 (data not shown).
FrsA Increases Accumulation of Fermentation Products—
From repeated measurements of growth and glucose consumption, we found that the frsA strain (BM101) harboring pKY513
consumed glucose slightly faster than the wild type (MG1655)
or BM101 harboring pBR322, while MG1655 showed a slightly
faster growth rate than the other strains in M9 medium containing glucose as the sole carbon source under aerobic culture
conditions. Fig. 6 shows representative data of several independent reproducible experiments. Since either disruption or
higher expression of FrsA does not affect the expression level of
IIAGlc or IICBGlc (data not shown), these results suggest that
FrsA might affect metabolic flux through the glycolytic pathway. It should also be noted that color changes in PM analyses
do not always correlate with growth rate, because cell respiration is not necessarily coupled to cell growth (21).
To elucidate the reason for the discrepancy between growth
and glucose utilization rates in these strains, we carried out 1H
NMR spectroscopy of the growth medium after 12 h (Figs. 7, A
and B). More acetate, ethanol, lactate, pyruvate, and succinate
were accumulated in the glucose medium for BM101 harboring
pKY513 than for MG1655 (Fig. 7A). BM101, in which the frsA
gene is deleted, produced lower amounts of these fermentation
products than did the wild type. In the growth medium containing fructose as the sole carbon source, production of acetate, ethanol, formate, and lactate increased in strain BM101
harboring pKY513 (Fig. 7B).
Enzymatic analyses of the concentration of acetate in 20 h
growth medium also confirmed these data (Fig. 7C). The concentration of acetate from BM101 harboring pKY513 was significantly higher than MG1655 or BM101 harboring pBR322 in
synthetic medium containing all the indicated carbon sources
except for glycerol. These results suggest that FrsA stimulates
mixed-acid fermentation in E. coli by acting as a fermentation/
respiration switch that down-regulates respiration and up-regulates fermentation rates.
31619
31620
Interaction of Enzyme IIAGlc with FrsA
immobilized HPr in the BIAcore in our previous study (8) or
when purified HPr or IIBGlc were exposed to the IIAGlc surface
in this study (data not shown), although the association con-
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FIG. 7. Analysis of fermentation products in culture medium.
In A and B, cells of BM101/pBR322 (a), MG1655/pBR322 (b), and
BM101/pKY513 (c) were grown aerobically in M9 minimal medium
supplemented with 0.2% casamino acids and 0.5% glucose (A) as in Fig.
6 or 0.5% fructose (B). After incubation for 12 h at 37 °C, cell-free
culture medium was mixed with 0.10 volume of D2O to obtain 1H NMR
spectra of fermentation products. Metabolic products are abbreviated:
A, acetate; E, ethanol; F, formate; L, lactate; P, pyruvate; and S,
succinate. C, concentration of acetate accumulated in medium containing indicated carbon sources (0.5%) was determined 20 h after cells
were inoculated as described under “Experimental Procedures.” Blank
bar, BM101/pBR322; hatched bar, MG1655/pBR322; and filled
bar, BM101/pKY513.
stants were reported to be ⬃1.5 ⫻ 105 M⫺1 between EI and HPr
(27) and ⬃105 M⫺1 between HPr and IIAGlc (28) and between
IIAGlc and IIBGlc (29). We concluded that it was unlikely that
an interaction with a KA in this range would be detected by
SPR under our experimental conditions.
The search for IIAGlc-interacting protein(s) employing ligand-fishing by SPR in the absence of Zn2⫹ ion turned up a
protein with the expected tight binding; after purification to
near homogeneity, sequencing experiments identified the novel
IIAGlc-interacting protein as the product specified by the yafA
gene, which we now refer to as frsA (Fig. 1B). This gene was
previously only characterized as encoding a hypothetical protein of unknown function. Sequence comparison of the protein
with other proteins (HPr, IIBGlc, glycerol kinase, lactose permease, and melibiose permease) interacting with IIAGlc revealed no homology.
We cloned the frsA gene under the control of the PL promoter to produce the expression vector, pKY103. Consequently,
FrsA could be overexpressed to about 30% of the total cell
protein. Purification of the overexpressed protein, using a combination of several chromatographic procedures, resulted in
more than 98% pure protein (data not shown). The interaction
between FrsA and IIAGlc was dependent on the phosphorylation state of IIAGlc, and only the dephospho-form of IIAGlc
showed an interaction with FrsA, both in vitro and in vivo
(Figs. 2– 4).
A BLAST search revealed that orthologs of FrsA exist only in
some Gram-negative bacteria such as E. coli, Salmonella typhimurium, Shigella flexneri, Yersinia pestis, Vibrio cholerae,
Vibrio vulnificus, Vibrio parahemeolyticus, and Photorhabdus
luminescens (amino acid sequences at www.ncbi.nlm.nih.gov).
It is worthy of note that all of these species are facultative
anaerobes belonging to the ␥-proteobacterial group, and most of
them are highly pathogenic. FrsA has been referred to as a
hypothetical protein and studies related to this protein have
not previously been reported. Thus, elucidation of the function
of FrsA might help us understand the physiology of facultative
anaerobiosis and pathogenesis in these strains. A BLAST conserved domain search suggested that FrsA might be an enzyme
of the ␣/ hydrolase family, which includes dienlactone hydrolase, dipeptidyl aminopeptidase, and acetyl esterase. With purified FrsA, however, we could not show any of these enzymatic
activities nor find the consensus sequence patterns for these
enzymes from the FrsA amino acid sequence.
It is noteworthy that the cellular concentration of FrsA is
about 0.27 M while that of IIAGlc is about 40 M (30). Since
there is a tight interaction of the two proteins, it is likely that
all of the cellular FrsA protein exists in a complex with IIAGlc
when PTS sugars are being transported (the condition under
which the PTS proteins are mainly in their dephospho-forms).
Thus we assumed that it probably interfaces with the status of
phosphorylation of the PTS.
For facultative anaerobes like E. coli, fine tuning between
respiratory and fermentation pathways may be important for
more efficient utilization of sugars available in the environment. To test the physiological importance of the interaction
between FrsA and IIAGlc, we prepared E. coli strains with
different genotypes. Since the precise function of FrsA has not
been elucidated and its orthologs are found only in facultative
anaerobes, we carried out PM experiments. This technology
can be used to find new functions of genes by testing mutants
for a large number of phenotypes simultaneously using cell
respiration as the reporter system (22). Based on phenotype
microarray experiments (Fig. 5), it was found that disruption of
frsA resulted in an increase of cellular respiration in medium
containing glucose or some other sugars as the sole carbon
Interaction of Enzyme IIAGlc with FrsA
Acknowledgment—We thank Dr. Susan Gottesman (National Institutes of Health, Bethesda, MD) for the kind gift of E. coli DY330.
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source compared with wild type; introduction of pKY513 into
the BM101 strain resulted in the same phenotype as the wild
type MG1655, indicating that increased respiration rate of
BM101 on several sugars was correlated with deletion of the
frsA gene from MG1655 (data not shown). NMR spectroscopic
and enzymatic analyses showed that more fermentation products are accumulated in the growth medium of the pKY513harboring strain than the BM101 and MG1655 strains despite
thorough aeration with atmospheric air (Fig. 7). Since all PTS
and several non-PTS sugars can also lower the phosphorylated
forms of the PTS proteins (7), the specific interaction between
dephosphorylated IIAGlc and FrsA may regulate FrsA activity
to balance utilization of sugars through fermentative or respiratory pathways in Gram-negative bacteria.
Data in this study suggest that FrsA promotes fermentation
and that IIAGlc complexes with it to negate the effect. A previous study reported that the modulation in the synthesis of
specific proteins during aerobic glucose starvation is similar to
the response of cells shifted to anaerobiosis in that the glucosestarved cell increases the flow of metabolites through glycolysis
to fermentative pathways although the trigger mechanisms
could not be elucidated (31). The present studies may provide
the molecular mechanism for this phenomenon. When cells are
starved of glucose or other energy-rich carbon sources, IIAGlc
will exist in its phospho-form, and the FrsA protein would be
depleted of IIAGlc and thus be fully active. FrsA as a downregulator of respiration or up-regulator of fermentation should
make cells increase the flow of metabolites into fermentative
pathways. Increased excretion of acetate, lactate, pyruvate,
succinate, ethanol, and formate in cells harboring pKY513 supports this model. Conservation of amino acid sequences of
IIAGlc and FrsA in facultative anaerobes suggests that regulation of the metabolic switch from respiration to fermentative
pathways during aerobic glucose starvation by IIAGlc might be
a general mechanism in facultative anaerobes. Further experiments will be necessary to define the precise metabolic reaction(s) influenced by FrsA, subject to regulation by IIAGlc.
31621
A Novel Fermentation/Respiration Switch Protein Regulated by Enzyme IIAGlc in
Escherichia coli
Byoung-Mo Koo, Mi-Jeong Yoon, Chang-Ro Lee, Tae-Wook Nam, Young-Jun Choe,
Howard Jaffe, Alan Peterkofsky and Yeong-Jae Seok
J. Biol. Chem. 2004, 279:31613-31621.
doi: 10.1074/jbc.M405048200 originally published online May 28, 2004
Access the most updated version of this article at doi: 10.1074/jbc.M405048200
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