Complete Genome Sequence of Enterovirus D68 Detected in Classroom Air and on Environmental Surfaces

John A Lednicky; Tania S Bonny; J Glenn Morris; Julia C Loeb

doi:10.1128/genomeA.00579-16

Complete Genome Sequence of Enterovirus D68 Detected in Classroom Air and on Environmental Surfaces

Genome Announc. 2016 Jun 16;4(3):e00579-16. doi: 10.1128/genomeA.00579-16.

Authors

John A Lednicky¹, Tania S Bonny², J Glenn Morris³, Julia C Loeb²

Affiliations

¹ Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA [email protected].
² Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA.
³ Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA Department of Medicine, College of Medicine, University of Florida, Gainesville, Florida, USA.

Abstract

We amplified and sequenced the complete genome of enterovirus D68 (EV-D68) that had been collected from classroom air using a filter-based air sampling method and by swab sampling of environmental surfaces. Relatively high levels of EV-D68 genome equivalents were found per cubic meter of air by quantitative real-time reverse transcription-PCR (RT-PCR).

Grants and funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.