Genomic Characterization of Yogue, Kasokero, Issyk-Kul, Keterah, Gossas, and Thiafora Viruses: Nairoviruses Naturally Infecting Bats, Shrews, and Ticks

Am J Trop Med Hyg. 2015 Nov;93(5):1041-51. doi: 10.4269/ajtmh.15-0344. Epub 2015 Aug 31.

Abstract

The genus Nairovirus of arthropod-borne bunyaviruses includes the important emerging human pathogen, Crimean-Congo hemorrhagic fever virus (CCHFV), as well as Nairobi sheep disease virus and many other poorly described viruses isolated from mammals, birds, and ticks. Here, we report genome sequence analysis of six nairoviruses: Thiafora virus (TFAV) that was isolated from a shrew in Senegal; Yogue (YOGV), Kasokero (KKOV), and Gossas (GOSV) viruses isolated from bats in Senegal and Uganda; Issyk-Kul virus (IKV) isolated from bats in Kyrgyzstan; and Keterah virus (KTRV) isolated from ticks infesting a bat in Malaysia. The S, M, and L genome segments of each virus were found to encode proteins corresponding to the nucleoprotein, polyglycoprotein, and polymerase protein of CCHFV. However, as observed in Leopards Hill virus (LPHV) and Erve virus (ERVV), polyglycoproteins encoded in the M segment lack sequences encoding the double-membrane-spanning CCHFV NSm protein. Amino acid sequence identities, complement-fixation tests, and phylogenetic analysis indicated that these viruses cluster into three groups comprising KKOV, YOGV, and LPHV from bats of the suborder Yingochiroptera; KTRV, IKV, and GOSV from bats of the suborder Yangochiroptera; and TFAV and ERVV from shrews (Soricomorpha: Soricidae). This reflects clade-specific host and vector associations that extend across the genus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bunyaviridae Infections / epidemiology
  • Bunyaviridae Infections / virology*
  • Chiroptera / virology*
  • Genome, Viral / genetics*
  • Genomics
  • Humans
  • Kyrgyzstan / epidemiology
  • Malaysia / epidemiology
  • Nairovirus / classification
  • Nairovirus / genetics*
  • Nairovirus / isolation & purification
  • Nucleoproteins / genetics
  • Phylogeny
  • Senegal / epidemiology
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Shrews / virology*
  • Ticks / virology*
  • Uganda / epidemiology
  • Viral Proteins / genetics

Substances

  • Nucleoproteins
  • Viral Proteins