[Detection of the Bartonella DNA by the method of nested PCR in patients after tick bites in Novosibirsk region]

Mol Gen Mikrobiol Virusol. 2005:(4):14-7.
[Article in Russian]

Abstract

Arthropod-borne bacterial pathogen Bartonella DNA was detected in human blood cells after tick bites in summer 2003 and 2004 in Novosibirsk region by nested PCR with primers specific to groEL gene of HSP60 protein. Comparative assay of 190 p.b. of long PCR fragment revealed that the nucleotide sequences might belong to Bartonella henselae and Bartonella quintana.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Bartonella / genetics*
  • Bartonella / isolation & purification
  • Bartonella Infections / etiology
  • Bartonella Infections / microbiology*
  • Bartonella henselae / genetics
  • Bartonella henselae / pathogenicity
  • Bartonella quintana / genetics
  • Bartonella quintana / pathogenicity
  • Bites and Stings
  • Blood / microbiology
  • DNA, Bacterial / analysis*
  • Humans
  • Polymerase Chain Reaction / methods*
  • Siberia
  • Ticks

Substances

  • DNA, Bacterial