Papers by domenico scionti
Clinical Chemistry, 2005
Bookmarks Related papers MentionsView impact
Cellular Reprogramming, Dec 1, 2017
Neural crest-derived mesenchymal stem cells (MSCs) obtained from dental tissues received consider... more Neural crest-derived mesenchymal stem cells (MSCs) obtained from dental tissues received considerable interest in regenerative medicine, particularly in nerve regeneration owing to their embryonic origin and ease of harvest. Proliferation efficacy and differentiation capacity into diverse cell lineages propose dental MSCs as an in vitro tool for disease modeling. In this study, we investigated the spontaneous differentiation efficiency of dental MSCs obtained from human gingiva tissue (hGMSCs) into neural progenitor cells after extended passaging. At passage 41, the morphology of hGMSCs changed from typical fibroblast-like shape into sphere-shaped cells with extending processes. Next-generation transcriptomics sequencing showed increased expression of neural progenitor markers such as NES, MEIS2, and MEST. In addition, de novo expression of neural precursor genes, such as NRN1, PHOX2B, VANGL2, and NTRK3, was noticed in passage 41. Immunocytochemistry results showed suppression of neurogenesis repressors TP53 and p21, whereas Western blot results revealed the expression of neurotrophic factors BDNF and NT3 at passage 41. Our results showed the spontaneous efficacy of hGMSCs to differentiate into neural precursor cells over prolonged passages and that these cells may assist in producing novel in vitro disease models that are associated with neural development.
Bookmarks Related papers MentionsView impact
Cell Transplantation, Jan 14, 2019
Bookmarks Related papers MentionsView impact
Journal of Biomedical Materials Research Part A, Sep 26, 2017
Bookmarks Related papers MentionsView impact
International Journal of Molecular Sciences, Jan 23, 2018
Bookmarks Related papers MentionsView impact
Cellular Reprogramming, Jun 1, 2017
Bookmarks Related papers MentionsView impact
Supplemental Material, Table_S2(2) for The Role of Hypoxia on the Neuronal Differentiation of Gin... more Supplemental Material, Table_S2(2) for The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study by Agnese Gugliandolo, Francesca Diomede, Domenico Scionti, Placido Bramanti, Oriana Trubiani, and Emanuela Mazzon in Cell Transplantation
Bookmarks Related papers MentionsView impact
Table_S2 for The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cel... more Table_S2 for The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study by Agnese Gugliandolo, Francesca Diomede, Domenico Scionti, Placido Bramanti, Oriana Trubiani and Emanuela Mazzon in Cell Transplantation
Bookmarks Related papers MentionsView impact
Supplemental Material, Table_S2(1) for The Role of Hypoxia on the Neuronal Differentiation of Gin... more Supplemental Material, Table_S2(1) for The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study by Agnese Gugliandolo, Francesca Diomede, Domenico Scionti, Placido Bramanti, Oriana Trubiani, and Emanuela Mazzon in Cell Transplantation
Bookmarks Related papers MentionsView impact
Table_S1_(1) for The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem... more Table_S1_(1) for The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study by Agnese Gugliandolo, Francesca Diomede, Domenico Scionti, Placido Bramanti, Oriana Trubiani and Emanuela Mazzon in Cell Transplantation
Bookmarks Related papers MentionsView impact
Gene analysis. Differential regulation of genes coding for adhesion molecules and ECM proteins in... more Gene analysis. Differential regulation of genes coding for adhesion molecules and ECM proteins in the 3D-PLA + PEI-EVs + hGMSCs group compared with hGMSCs cells. Upregulated transcripts shown in red, downregulated transcripts shown in green (q ≤ 0.05, Benjamini–Hochberg false discovery rate). (JPEG 271 kb)
Bookmarks Related papers MentionsView impact
Gene expression. Expression value of genes activated during osteogenesis and osteoblast different... more Gene expression. Expression value of genes activated during osteogenesis and osteoblast differentiation in 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs and compared with hGMSCs (q ≤ 0.05, Benjamini–Hochberg false discovery rate). (JPEG 319 kb)
Bookmarks Related papers MentionsView impact
Table S1. The differential gene expression between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGM... more Table S1. The differential gene expression between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs compared with hGMSCs is given as expression value and fold change expressed in logarithm with base 2 (FC Log2). Gene ontology (GO) processes indicate the gene classification in the "regulation of ossification" and "ossification". Instead, the statistical significance is indicated by the false discovery rate (FDR), q values ≤ 0.05 were considered statistically significant. Table S2. The differential gene expression between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs compared with hGMSCs is given as expression value and fold change expressed in logarithm with base 2 (FC Log2). Gene ontology (GO) processes indicate the gene classification in the "regulation of osteoblast differentiation" and "osteoblast differentiation". Instead, the statistical significance is indicated by the false discovery rate (FDR), q values ≤ 0.05 were considered stat...
Bookmarks Related papers MentionsView impact
Gene expression. (A) The expression value of genes for "regulation of ossification" and... more Gene expression. (A) The expression value of genes for "regulation of ossification" and "ossification" differentially expressed between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs and compared with hGMSCs. (B) The expression value of genes for "regulation of osteoblast differentiation" and "osteoblast differentiation" differentially expressed between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs and compared with hGMSCs. (JPEG 769 kb)
Bookmarks Related papers MentionsView impact
3D PLA and hGMSCs interactions. SEM micrographs at low (A) and high magnifications (B) showing ce... more 3D PLA and hGMSCs interactions. SEM micrographs at low (A) and high magnifications (B) showing cell adhesion on the scaffold surface. Scaffold surface without hGMSCs are reported in the inset in section A. A, magnification 300×; B, magnification 750×. Scale bar = 10 μm. (JPEG 1188 kb)
Bookmarks Related papers MentionsView impact
Cell Transplantation, 2019
Mesenchymal stem cells (MSCs) are widely used in stem cell therapy for regenerative purposes. Ora... more Mesenchymal stem cells (MSCs) are widely used in stem cell therapy for regenerative purposes. Oral-derived MSCs, such as gingival MSCs (GMSCs), deriving from the neural crest seem more suitable to differentiate toward the neuronal lineage. In addition, the preconditioning of MSCs may improve their beneficial effects. Since it is known that hypoxia may influence stem cell properties, we were interested in evaluating the effects of hypoxia preconditioning on the neuronal differentiation of GMSCs. With this aim, we evaluated the transcriptional profile of GMSCs exposed to basal and neuroinductive medium both in normoxia and in cells preconditioned for 48 h in hypoxia. We compared their transcriptional profile using Next Generation Sequencing. At first we observed that hypoxia did not alter cell morphology compared with the GMSCs cultured in a normoxic condition. In order to understand hypoxia preconditioning effects on neuronal differentiation, we screened genes with Log2 fold change ≥...
Bookmarks Related papers MentionsView impact
International Journal of Molecular Sciences, 2018
Periodontal ligament mesenchymal stem cells (hPDLSCs), as well as all mesenchymal stem cells, sho... more Periodontal ligament mesenchymal stem cells (hPDLSCs), as well as all mesenchymal stem cells, show self-renewal, clonogenicity, and multi-tissue differentiation proprieties and can represent a valid support for regenerative medicine. We treated hPDLSCs with a combination of Moringin (MOR) and Cannabidiol (CBD), in order to understand if treatment could improve their survival and their in vitro differentiation capacity. Stem cells survival is fundamental to achieve a successful therapy outcome in the re-implanted tissue of patients. Through NGS transcriptome analysis, we found that combined treatment increased hPDLSCs survival, by inhibition of apoptosis as demonstrated by enhanced expression of anti-apoptotic genes and reduction of pro-apoptotic ones. Moreover, we investigated the possible involvement of PI3K/Akt/mTOR pathway, emphasizing a differential gene expression between treated and untreated cells. Furthermore, hPDLSCs were cultured for 48 h in the presence or absence of CBD ...
Bookmarks Related papers MentionsView impact
Cellular reprogramming, Dec 1, 2017
Neural crest-derived mesenchymal stem cells (MSCs) obtained from dental tissues received consider... more Neural crest-derived mesenchymal stem cells (MSCs) obtained from dental tissues received considerable interest in regenerative medicine, particularly in nerve regeneration owing to their embryonic origin and ease of harvest. Proliferation efficacy and differentiation capacity into diverse cell lineages propose dental MSCs as an in vitro tool for disease modeling. In this study, we investigated the spontaneous differentiation efficiency of dental MSCs obtained from human gingiva tissue (hGMSCs) into neural progenitor cells after extended passaging. At passage 41, the morphology of hGMSCs changed from typical fibroblast-like shape into sphere-shaped cells with extending processes. Next-generation transcriptomics sequencing showed increased expression of neural progenitor markers such as NES, MEIS2, and MEST. In addition, de novo expression of neural precursor genes, such as NRN1, PHOX2B, VANGL2, and NTRK3, was noticed in passage 41. Immunocytochemistry results showed suppression of ne...
Bookmarks Related papers MentionsView impact
Medicine, 2018
The aromatic L-amino acid decarboxylase (AADC) deficiency (AADCD) is a rare, autosomal recessive ... more The aromatic L-amino acid decarboxylase (AADC) deficiency (AADCD) is a rare, autosomal recessive neurometabolic disorder caused by a deficit of the AADC that is involved in serotonin and dopamine biosynthesis, causing as a consequence, their deficits, but also a lack of norepinephrine and epinephrine, given that dopamine is their precursor. We report the case of a Caucasian 43-year-old woman heterozygous for p.Ser250Phe in DDC, encoding for AADC with a positive family history for behavioral problems. Since adolescence, she manifested behavioral abnormalities. Three months before the admission to our hospital, she presented with a permanent dystonic posture at the 4 limbs with numbness and tingling, diplopia, and low potassium levels. She was treated with muscle relaxants and potassium, but with no results. Olanzapine was administrated, worsening mood problems. Later, after fever, low potassium levels, and increased difficulty to move, she was admitted to the neurology unit where, af...
Bookmarks Related papers MentionsView impact
Scientific reports, Jan 14, 2018
The therapeutic strategies for neurodegenerative diseases still represent a vast research field b... more The therapeutic strategies for neurodegenerative diseases still represent a vast research field because of the lack of targeted, effective and resolutive treatment for neurodegenerative diseases. The use of stem cell-based therapy is an alternative approach that could lead to the replacement of damaged neuronal tissue. For this purpose, adult mesenchymal stem cells (MSC), including periodontal ligament stem cells (PDLSCs), could be very useful for their differentiation capacity, easy isolation and the ability to perform an autologous implant. The aim of this work was to test whether the Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate; GMG-ITC], an isothiocyanate extracted from Moringa oleifera seeds, was able to induce PDLSCs toward neural progenitor differentiation. Next-generation transcriptomics sequencing showed that moringin treatment increased the expression of genes involved in neuron cortical development and in particular in neuron belonging to upper and deep cortical l...
Bookmarks Related papers MentionsView impact
Uploads
Papers by domenico scionti