Экcпеpиментально доказано, что пpи cемейныx фоpмаx болезни Альцгеймеpа мутации в гене пpеcенилина... more Экcпеpиментально доказано, что пpи cемейныx фоpмаx болезни Альцгеймеpа мутации в гене пpеcенилина 1 могут пpиводить к наpушению пpоцеccов клеточной адгезии в модельныx клеточныx культуpаx. Показано, что в L-фибpоблаcтаx мышей cо cтабильной экcпpеccией GFP-PS1 кДНК, cодеpжащей мутации G209V или Е319G, наpушено межклеточное взаимодейcтвие и накопление GFP-PS1 кДНК в межклеточныx контактаx. Близкие pезультаты были получены и в тpанcфециpованныx эпителиальныx клеткаx человека HEp 2. Пpедполагаетcя, что мутации пpи cемейныx фоpмаx болезни Альцгеймеpа пpиводят к наpушению функций пpеcенилина 1 в клеточной адгезии.
<p>Immunohistochemistry with antibody to biotin: Left panels - Bar, 50 µm; Right panels-Bar... more <p>Immunohistochemistry with antibody to biotin: Left panels - Bar, 50 µm; Right panels-Bar, 10 µm. a,b - Antp-COG133 (COG112) - RQIKIWFQNRRMKWKK LRVRLASHLRKLRKRLL. c,d- CF (fragment 142–153 of human growth factor U2AF)-SQMTRQARRLYV, control peptide, which does not cross BBB <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008191#pone.0008191-Peng1" target="_blank">[33]</a>; e,f – no peptides added.</p
<p>The Learning Index was calculated as described in “<a href="http://www.plosone.o... more <p>The Learning Index was calculated as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008191#s4" target="_blank">Materials and Methods</a>”. All scores are expressed as mean±SEM, (n = 6) where (n) is the number of independent assays. Asterisk (*) indicates control transgenic lines. Statistical analysis was performed using one-way ANOVA and Tukey-Kramer multiple comparison post hoc test. Significance was accepted at p<0.05.</p><p>Groups of flies showing statistically significant differences from controls in learning index are shown in bold font.</p
Gastric cancer is the fifth most common malignancy and the third leading cause of cancer-related ... more Gastric cancer is the fifth most common malignancy and the third leading cause of cancer-related deaths worldwide. Chemotherapies against gastric cancer often fail, with cancer recurrence due potentially to the persistence of cancer stem cells. This unique subpopulation of cells in tumors possesses the ability to self-renew and dedifferentiate. These cancer stem cells are critical for initiation, maintenance, metastasis, and relapse of cancers; however, the molecular mechanisms supporting cancer stemness remain largely unknown. Increased kinase and decreased phosphatase activity are hallmarks of oncogenic signaling. Protein phosphatase 2A (PP2A) functions as a tumor-suppressor enzyme, and elevated levels of SET/I2PP2A, an endogenous PP2A protein inhibitor, are correlated with poor prognosis of several human cancers. Here, it was determined that SET expression was elevated in tumor tissue in a gastric cancer mouse model system, and SET expression was positively correlated with poor s...
Amyloid peptide precursor (APP) gene expression increases in the brains of patients with Alzheime... more Amyloid peptide precursor (APP) gene expression increases in the brains of patients with Alzheimer&#39;s disease (AD). Regulation of expression at the transcriptional and posttranscriptional levels contributes significantly to these increases. Polypeptide hormones stimulate the promoter of the APP gene resulting in increased levels of heterogeneous nuclear RNAs (hnRNA). Through splicing, these hnRNAs are processed into 3 or more mature APP RNAs. The amounts of each of these similar but nonidentical APP RNAs may yield ratios of APP proteins which are required for neuronal survival, pathogenesis of neurons and/or produce phenocopies of learning and memory mutants of Drosophila.
Экcпеpиментально доказано, что пpи cемейныx фоpмаx болезни Альцгеймеpа мутации в гене пpеcенилина... more Экcпеpиментально доказано, что пpи cемейныx фоpмаx болезни Альцгеймеpа мутации в гене пpеcенилина 1 могут пpиводить к наpушению пpоцеccов клеточной адгезии в модельныx клеточныx культуpаx. Показано, что в L-фибpоблаcтаx мышей cо cтабильной экcпpеccией GFP-PS1 кДНК, cодеpжащей мутации G209V или Е319G, наpушено межклеточное взаимодейcтвие и накопление GFP-PS1 кДНК в межклеточныx контактаx. Близкие pезультаты были получены и в тpанcфециpованныx эпителиальныx клеткаx человека HEp 2. Пpедполагаетcя, что мутации пpи cемейныx фоpмаx болезни Альцгеймеpа пpиводят к наpушению функций пpеcенилина 1 в клеточной адгезии.
<p>Immunohistochemistry with antibody to biotin: Left panels - Bar, 50 µm; Right panels-Bar... more <p>Immunohistochemistry with antibody to biotin: Left panels - Bar, 50 µm; Right panels-Bar, 10 µm. a,b - Antp-COG133 (COG112) - RQIKIWFQNRRMKWKK LRVRLASHLRKLRKRLL. c,d- CF (fragment 142–153 of human growth factor U2AF)-SQMTRQARRLYV, control peptide, which does not cross BBB <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008191#pone.0008191-Peng1" target="_blank">[33]</a>; e,f – no peptides added.</p
<p>The Learning Index was calculated as described in “<a href="http://www.plosone.o... more <p>The Learning Index was calculated as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008191#s4" target="_blank">Materials and Methods</a>”. All scores are expressed as mean±SEM, (n = 6) where (n) is the number of independent assays. Asterisk (*) indicates control transgenic lines. Statistical analysis was performed using one-way ANOVA and Tukey-Kramer multiple comparison post hoc test. Significance was accepted at p<0.05.</p><p>Groups of flies showing statistically significant differences from controls in learning index are shown in bold font.</p
Gastric cancer is the fifth most common malignancy and the third leading cause of cancer-related ... more Gastric cancer is the fifth most common malignancy and the third leading cause of cancer-related deaths worldwide. Chemotherapies against gastric cancer often fail, with cancer recurrence due potentially to the persistence of cancer stem cells. This unique subpopulation of cells in tumors possesses the ability to self-renew and dedifferentiate. These cancer stem cells are critical for initiation, maintenance, metastasis, and relapse of cancers; however, the molecular mechanisms supporting cancer stemness remain largely unknown. Increased kinase and decreased phosphatase activity are hallmarks of oncogenic signaling. Protein phosphatase 2A (PP2A) functions as a tumor-suppressor enzyme, and elevated levels of SET/I2PP2A, an endogenous PP2A protein inhibitor, are correlated with poor prognosis of several human cancers. Here, it was determined that SET expression was elevated in tumor tissue in a gastric cancer mouse model system, and SET expression was positively correlated with poor s...
Amyloid peptide precursor (APP) gene expression increases in the brains of patients with Alzheime... more Amyloid peptide precursor (APP) gene expression increases in the brains of patients with Alzheimer&#39;s disease (AD). Regulation of expression at the transcriptional and posttranscriptional levels contributes significantly to these increases. Polypeptide hormones stimulate the promoter of the APP gene resulting in increased levels of heterogeneous nuclear RNAs (hnRNA). Through splicing, these hnRNAs are processed into 3 or more mature APP RNAs. The amounts of each of these similar but nonidentical APP RNAs may yield ratios of APP proteins which are required for neuronal survival, pathogenesis of neurons and/or produce phenocopies of learning and memory mutants of Drosophila.
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