1. The coenzyme preference of bovine liver glutamate dehydrogenase (GDH) was probed using dual wa... more 1. The coenzyme preference of bovine liver glutamate dehydrogenase (GDH) was probed using dual wavelength spectroscopy and pairing the thionicotinamide analogues, S-NAD or S-NADP (which have absorbance maxima at 400 nm), with the natural coenzymes, NADP or NAD. 2. S-NAD and S-NADP were found to be good alternate substrates for GDH: the apparent Km's for the thioderivatives were similar to those of the corresponding natural coenzymes, the apparent Km's for glutamate were unaltered by the substitution of the thioderivatives, and the effects of inhibitors and activators on S-NAD or S-NADP kinetics were qualitatively the same as those found for NAD or NADP, respectively. 3. Dual wavelength assays paired NAD and S-NADP or S-NAD and NADP to study the simultaneous reduction of the two coenzymes. Conditions of increasing glutamate concentrations produced differential effects on the rates of the NAD vs NADP reactions, the result, with either nucleotide pair, promoting the NADP linked...
Comparative biochemistry and physiology. A, Comparative physiology, 1986
The ability to tolerate extracellular freezing as an adaptation for winter survival was tested in... more The ability to tolerate extracellular freezing as an adaptation for winter survival was tested in seven species of terrestrially-hibernating amphibians found in eastern Canada. All species had only moderate supercooling abilities, with whole animal supercooling points of -1.5 to -3 degrees C. Two salamander species, Plethodon cinereus and Ambystoma laterale, and the toad, Bufo americanus, were freezing intolerant and were killed when frozen for 24 hr at temperatures just below their supercooling points. The major winter strategy of these animals appears to behavioural avoidance of subzero temperatures. Four species of frogs Rana sylvatica, Hyla versicolor, Hyla crucifer and Pseudacris triseriata, survived extracellular freezing at moderate subzero temperatures (-2 to -4 degrees C) for periods of time ranging up to 2 weeks. All four frog species accumulated low molecular weight carbohydrates as cryoprotectants, glycerol being the major cryoprotectant in adult H. versicolor, while imm...
Comparative biochemistry and physiology. B, Comparative biochemistry, 1979
1. The malate-aspartate cycle was demonstrable in subcellular preparations of hearts from Arapaim... more 1. The malate-aspartate cycle was demonstrable in subcellular preparations of hearts from Arapaima, Lepidosiren, and Synbranchus (obligate air breathers), Hoplerythriunus (facultative air breather), and Osteoglossum and Hoplias (obligate water breathers). 2. Although no respiratory evidence for significant alpha-glycerophosphate cycle participation could be shown in the air breathers, this cycle was demonstrable in hearts of water breathers. 3. In agreement with the O2 uptake studies, it was possible to reconstruct the malate-aspartate, but not the alpha-glycerophosphate cycle, in isolated mitochondria from air breathers, while both shuttles could be reconstructed with heart mitochondria in the case of water breathing fishes.
Comparative biochemistry and physiology. B, Comparative biochemistry, 1979
1. Using subcellular preparations of gills from Arapaima, an obligate air breather, and aruana, a... more 1. Using subcellular preparations of gills from Arapaima, an obligate air breather, and aruana, a related osteoglossid that is an obligate water breather, a comparison was made of the relative roles of the malate-aspartate cycle and the alpha-glycerophosphate (alpha-GP) cycle in transferring reducing equivalents from the cytosol to the mitochondria. 2. In aruana gill preparations, the alpha-GP cycle could be most clearly demonstrated by reconstructing it with purified isolated mitochondria, using the oxidation rate of exogenous NADH as a measure of the cycling activity. 3. Subcellular preparations of Arapaima gill, in contrast to the aruana gill, were not responsive to exogenous alpha-glycerophosphate, but a glutamate-malate stimulated O2 uptake was sensitive to aminooxyacetate, an aminotransferase inhibitor, a result that would be expected if the respiration were based on malate-aspartate cycling. 4. It was concluded that, compared to the alpha-glycerophosphate cycle, the malate-as...
MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. I... more MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. In this study, we determined the expression and structural characteristics of 20 miRNAs in brown (BAT) and white adipose tissue (WAT) during torpor in thirteen-lined ground squirrels. Using a modified stem-loop technique, we found that during torpor, expression of six miRNAs including let-7a, let-7b, miR-107, miR-150, miR-222 and miR-31 was significantly downregulated in WAT (P<0.05), which was 16%-54% of euthermic non-torpid control squirrels, whereas expression of three miRNAs including miR-143, miR-200a and miR-519d was found to be upregulated by 1.32-2.34-fold. Similarly, expression of more miRNAs was downregulated in BAT during torpor. We detected reduced expression of 6 miRNAs including miR-103a, miR-107, miR-125b, miR-21, miR-221 and miR-31 (48%-70% of control), while only expression of miR-138 was significantly upregulated (2.91±0.8-fold of the control, P<0.05). Interestingl...
1. The coenzyme preference of bovine liver glutamate dehydrogenase (GDH) was probed using dual wa... more 1. The coenzyme preference of bovine liver glutamate dehydrogenase (GDH) was probed using dual wavelength spectroscopy and pairing the thionicotinamide analogues, S-NAD or S-NADP (which have absorbance maxima at 400 nm), with the natural coenzymes, NADP or NAD. 2. S-NAD and S-NADP were found to be good alternate substrates for GDH: the apparent Km's for the thioderivatives were similar to those of the corresponding natural coenzymes, the apparent Km's for glutamate were unaltered by the substitution of the thioderivatives, and the effects of inhibitors and activators on S-NAD or S-NADP kinetics were qualitatively the same as those found for NAD or NADP, respectively. 3. Dual wavelength assays paired NAD and S-NADP or S-NAD and NADP to study the simultaneous reduction of the two coenzymes. Conditions of increasing glutamate concentrations produced differential effects on the rates of the NAD vs NADP reactions, the result, with either nucleotide pair, promoting the NADP linked...
Comparative biochemistry and physiology. A, Comparative physiology, 1986
The ability to tolerate extracellular freezing as an adaptation for winter survival was tested in... more The ability to tolerate extracellular freezing as an adaptation for winter survival was tested in seven species of terrestrially-hibernating amphibians found in eastern Canada. All species had only moderate supercooling abilities, with whole animal supercooling points of -1.5 to -3 degrees C. Two salamander species, Plethodon cinereus and Ambystoma laterale, and the toad, Bufo americanus, were freezing intolerant and were killed when frozen for 24 hr at temperatures just below their supercooling points. The major winter strategy of these animals appears to behavioural avoidance of subzero temperatures. Four species of frogs Rana sylvatica, Hyla versicolor, Hyla crucifer and Pseudacris triseriata, survived extracellular freezing at moderate subzero temperatures (-2 to -4 degrees C) for periods of time ranging up to 2 weeks. All four frog species accumulated low molecular weight carbohydrates as cryoprotectants, glycerol being the major cryoprotectant in adult H. versicolor, while imm...
Comparative biochemistry and physiology. B, Comparative biochemistry, 1979
1. The malate-aspartate cycle was demonstrable in subcellular preparations of hearts from Arapaim... more 1. The malate-aspartate cycle was demonstrable in subcellular preparations of hearts from Arapaima, Lepidosiren, and Synbranchus (obligate air breathers), Hoplerythriunus (facultative air breather), and Osteoglossum and Hoplias (obligate water breathers). 2. Although no respiratory evidence for significant alpha-glycerophosphate cycle participation could be shown in the air breathers, this cycle was demonstrable in hearts of water breathers. 3. In agreement with the O2 uptake studies, it was possible to reconstruct the malate-aspartate, but not the alpha-glycerophosphate cycle, in isolated mitochondria from air breathers, while both shuttles could be reconstructed with heart mitochondria in the case of water breathing fishes.
Comparative biochemistry and physiology. B, Comparative biochemistry, 1979
1. Using subcellular preparations of gills from Arapaima, an obligate air breather, and aruana, a... more 1. Using subcellular preparations of gills from Arapaima, an obligate air breather, and aruana, a related osteoglossid that is an obligate water breather, a comparison was made of the relative roles of the malate-aspartate cycle and the alpha-glycerophosphate (alpha-GP) cycle in transferring reducing equivalents from the cytosol to the mitochondria. 2. In aruana gill preparations, the alpha-GP cycle could be most clearly demonstrated by reconstructing it with purified isolated mitochondria, using the oxidation rate of exogenous NADH as a measure of the cycling activity. 3. Subcellular preparations of Arapaima gill, in contrast to the aruana gill, were not responsive to exogenous alpha-glycerophosphate, but a glutamate-malate stimulated O2 uptake was sensitive to aminooxyacetate, an aminotransferase inhibitor, a result that would be expected if the respiration were based on malate-aspartate cycling. 4. It was concluded that, compared to the alpha-glycerophosphate cycle, the malate-as...
MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. I... more MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. In this study, we determined the expression and structural characteristics of 20 miRNAs in brown (BAT) and white adipose tissue (WAT) during torpor in thirteen-lined ground squirrels. Using a modified stem-loop technique, we found that during torpor, expression of six miRNAs including let-7a, let-7b, miR-107, miR-150, miR-222 and miR-31 was significantly downregulated in WAT (P<0.05), which was 16%-54% of euthermic non-torpid control squirrels, whereas expression of three miRNAs including miR-143, miR-200a and miR-519d was found to be upregulated by 1.32-2.34-fold. Similarly, expression of more miRNAs was downregulated in BAT during torpor. We detected reduced expression of 6 miRNAs including miR-103a, miR-107, miR-125b, miR-21, miR-221 and miR-31 (48%-70% of control), while only expression of miR-138 was significantly upregulated (2.91±0.8-fold of the control, P<0.05). Interestingl...
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Papers by Kenneth Storey