The present study was aimed to investigate the effect of photon flux density and silver nitrate e... more The present study was aimed to investigate the effect of photon flux density and silver nitrate either alone or in combination on in-vitro micro-propagation of banana cv. Grand Nain (AAA). Five different concentrations of silver nitrate (0 to 68 μM) and PFD (0-55 μmol m s) were assessed. Plantlets were grown in culture room at 24±2°C with 16±8 h photoperiod. Highest number of bud formation was observed in explant cultured in bud establishment medium (MBE) medium supplemented with AgNO3 (34 μM) and placed under dark condition. Additionally maximum bud break was observed in the pre developed buds exposed to PFD of 45 μmolms. Maximum shoot and root elongation was observed in shoot elongation medium supplemented with AgNO3 (34 μM) under 50 μmolm s of photon flux density. Light quality also affected the total chlorophyll and phenolic content. Incorporation of silver nitrate in the culture medium reduced tissue browning and precocious rooting. Darkness and low light intensity significantl...
Background: Setaria italica is an essential crop in the developing world and yield levels are low... more Background: Setaria italica is an essential crop in the developing world and yield levels are low. To improve the yield, the utilization of diverse germplasm in a breeding program is vital. Methods: The present study aimed to characterize 50 genotypes in the first year and out of 50, 10 genotypes in the second year using multivariant traits. Wide variation was observed in both the qualitative and quantitative characteristics. Analysis of variance revealed the presence of significant differences for most symptoms. Higher estimates of GCV were followed for grain yields, followed by panicle lengths and organic outcomes. Higher estimates of PCV were observed for plant height and leaf length, followed by leaf width. Low GCV and PCV were recorded as leaf length, 50% flowering in days. PCV study indicated direct selection based on characters, panicle weight, test weight and strawweight showed a high and positive effect on grain yield per plant in both rainy and summer season indicating the...
The objective of the present study is to evaluate the bioremediation potential of Eicchornia cras... more The objective of the present study is to evaluate the bioremediation potential of Eicchornia crassipes (bio-filters) inhabiting the wastewater bodies situated in the trans-region of the two rivers Ganges and Yamuna of Allahabad region. Amongst the physicochemical parameters measured viz. pH, DO, BOD and COD; pH decreased from 7.7 ± 0.02 to 7.3 ± 0.03 and from 8.8 ± 0.04 to 7.2 ± 0.01 in waste water bodies of trans-regions of the Yamuna and the Ganges, respectively. Similarly, DO values increased by 94% and 65%, BOD values decreased by 54% and 66% and COD values decreased by 53% and 73% respectively for trans- regions of the Yamuna and the Ganges rivers as compared to their respective control. The increase in total chlorophyll content was maximum (20%) in the trans-Yamuna as compared to trans-Ganges regions. Total protein content increased by 15% in shoots and 14% in roots of the plant in the trans-Yamuna regions over plants in the transGanges regions. These results indicate E. crass...
Summary Redox activities were compared in plasma membrane (PM) vesicles isolated by aqueous polym... more Summary Redox activities were compared in plasma membrane (PM) vesicles isolated by aqueous polymer twophase partitioning from the roots of chick pea seedlings (Cicer arietinum L. cv. Radhey) grown in hydroponic nutrient medium under deficiencies of boron or zinc. Seedlings raised under mineral-sufficient conditions served as control. The membrane preparations were highly purified and largely in right-side-out orientation as seen by marker enzyme assays, electron microscopy and latency studies of the PM marker, vanadate-sensitive K+-stimulated Mg2+-ATPase, with non-ionic detergents Triton X-100 and Brij 35. PM vesicles from boron and zinc deficient tissues showed lower ATPase and NAD(P)H-dependent redox activities vis-a-vis sufficient-tissue except under zinc deficiency where NADPH-dependent redox activity was higher. Quantitative inhibition of redox activities was seen by calmodulin antagonists (calmidazolium and W-7) in the presence of Triton X-100. The findings indicate that boron and zinc are essential for optimal function of PM ATPase and redox system(s) in chick pea.
... Gorakh Nath Gupta, Sanjeev Kumar Tiwari, Kapil Lawrence* and Reena S. Lawrence ... Canola oil... more ... Gorakh Nath Gupta, Sanjeev Kumar Tiwari, Kapil Lawrence* and Reena S. Lawrence ... Canola oil, animal fat, palm oil, corn oil, waste cooking oil (Felizardo et al., 2006; Kulkarni and Dalai, 2006) and Jatropha oil (Barnwal and Sharma, 2005) are other sources of commercial ...
The response to Cu 2+ exposure (10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on fifteen days post-em... more The response to Cu 2+ exposure (10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on fifteen days post-emergent Withania somnifera L. Dunal growing under natural environment in pots vis a vis Cu accumulation and antioxidative activity was studied on leaves, stems and roots. The duration of the experiment was for 2 months (Mid August-October, 2013) and interval between successive exposures was 7 days. As compared to control the following results were obtained. Copper (Cu 2+ ) content in plant tissues increased markedly with increasing soil Cu 2+ concentrations, and was highest in roots. Translocation factor (TF) and Bioconcentration Factor (BCF) values calculated were < 1, indicating a limited ability to accumulate and translocate Cu 2+ by the plant. Hydrogen peroxide formation was highest in roots and displayed a progressive increase in response to Cu 2+ exposure. With increasing Cu 2+ proline (non-enzymatic antioxidant) content was highest in leaves however, proline accumulation followe...
The effects of Cu +2 stress (0, 10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on growth and biochemic... more The effects of Cu +2 stress (0, 10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on growth and biochemical parameters of Withania somnifera L.Dunal, grown in a field pot experiment were studied. Increasing Cu +2 concentrations led to decreased stem length, root elongation and leaf area. Copper (Cu +2 ) stress decreased chlorophyll and carotenoids content in leaves and stems as compared to controls; this was more pronounced in leaves than in stems. Lipid peroxidation, in terms of malondialdehyde (MDA) content increased. Lipid peroxidation increased significantly with elevation in Cu +2 concentrations and was highest in roots followed by leaves and stems at 200 mM. Leakage of electrolytes measured in terms of membrane stability index (MSI), which showed a negative correlation with increasing Cu +2 levels. The MSI was lowest in 200 mM Cu +2 treated plants. The phenol content of leaves, stems and roots peaked at 50 mM Cu +2 treatment and thereafter declined. These results indicate that Cu + 2 ...
The present work is designed to evaluate the antioxidant, antibacterial activity and (TPC) total ... more The present work is designed to evaluate the antioxidant, antibacterial activity and (TPC) total phenolic content of Tagetes erecta flowers extracts. Antioxidant potential of flowers extracts was examined by applying three different methods. Antibacterial test was determined against gram positive and gram negative bacterium. The ethyl acetate extract had the greater phenolic concentration of 48.2 mg/g GAE followed by methanol extract which was 42.5 mg/g GAE and hexane extract 36.4 mg/g GAE. The ethyl acetate extract was found to be most effective in all the antioxidant methods in correlation with the TPC value. Moreover, all the flowers extracts are significantly effective in both Gram negative and Gram positive bacterium. Tagetes erecta flowers are hopefully considered as a promising source of natural pigments with a scope towards the alternative therapy for treatment of various health problems.
The present study was aimed to investigate the effect of photon flux density and silver nitrate e... more The present study was aimed to investigate the effect of photon flux density and silver nitrate either alone or in combination on in-vitro micro-propagation of banana cv. Grand Nain (AAA). Five different concentrations of silver nitrate (0 to 68 μM) and PFD (0-55 μmol m s) were assessed. Plantlets were grown in culture room at 24±2°C with 16±8 h photoperiod. Highest number of bud formation was observed in explant cultured in bud establishment medium (MBE) medium supplemented with AgNO3 (34 μM) and placed under dark condition. Additionally maximum bud break was observed in the pre developed buds exposed to PFD of 45 μmolms. Maximum shoot and root elongation was observed in shoot elongation medium supplemented with AgNO3 (34 μM) under 50 μmolm s of photon flux density. Light quality also affected the total chlorophyll and phenolic content. Incorporation of silver nitrate in the culture medium reduced tissue browning and precocious rooting. Darkness and low light intensity significantl...
Background: Setaria italica is an essential crop in the developing world and yield levels are low... more Background: Setaria italica is an essential crop in the developing world and yield levels are low. To improve the yield, the utilization of diverse germplasm in a breeding program is vital. Methods: The present study aimed to characterize 50 genotypes in the first year and out of 50, 10 genotypes in the second year using multivariant traits. Wide variation was observed in both the qualitative and quantitative characteristics. Analysis of variance revealed the presence of significant differences for most symptoms. Higher estimates of GCV were followed for grain yields, followed by panicle lengths and organic outcomes. Higher estimates of PCV were observed for plant height and leaf length, followed by leaf width. Low GCV and PCV were recorded as leaf length, 50% flowering in days. PCV study indicated direct selection based on characters, panicle weight, test weight and strawweight showed a high and positive effect on grain yield per plant in both rainy and summer season indicating the...
The objective of the present study is to evaluate the bioremediation potential of Eicchornia cras... more The objective of the present study is to evaluate the bioremediation potential of Eicchornia crassipes (bio-filters) inhabiting the wastewater bodies situated in the trans-region of the two rivers Ganges and Yamuna of Allahabad region. Amongst the physicochemical parameters measured viz. pH, DO, BOD and COD; pH decreased from 7.7 ± 0.02 to 7.3 ± 0.03 and from 8.8 ± 0.04 to 7.2 ± 0.01 in waste water bodies of trans-regions of the Yamuna and the Ganges, respectively. Similarly, DO values increased by 94% and 65%, BOD values decreased by 54% and 66% and COD values decreased by 53% and 73% respectively for trans- regions of the Yamuna and the Ganges rivers as compared to their respective control. The increase in total chlorophyll content was maximum (20%) in the trans-Yamuna as compared to trans-Ganges regions. Total protein content increased by 15% in shoots and 14% in roots of the plant in the trans-Yamuna regions over plants in the transGanges regions. These results indicate E. crass...
Summary Redox activities were compared in plasma membrane (PM) vesicles isolated by aqueous polym... more Summary Redox activities were compared in plasma membrane (PM) vesicles isolated by aqueous polymer twophase partitioning from the roots of chick pea seedlings (Cicer arietinum L. cv. Radhey) grown in hydroponic nutrient medium under deficiencies of boron or zinc. Seedlings raised under mineral-sufficient conditions served as control. The membrane preparations were highly purified and largely in right-side-out orientation as seen by marker enzyme assays, electron microscopy and latency studies of the PM marker, vanadate-sensitive K+-stimulated Mg2+-ATPase, with non-ionic detergents Triton X-100 and Brij 35. PM vesicles from boron and zinc deficient tissues showed lower ATPase and NAD(P)H-dependent redox activities vis-a-vis sufficient-tissue except under zinc deficiency where NADPH-dependent redox activity was higher. Quantitative inhibition of redox activities was seen by calmodulin antagonists (calmidazolium and W-7) in the presence of Triton X-100. The findings indicate that boron and zinc are essential for optimal function of PM ATPase and redox system(s) in chick pea.
... Gorakh Nath Gupta, Sanjeev Kumar Tiwari, Kapil Lawrence* and Reena S. Lawrence ... Canola oil... more ... Gorakh Nath Gupta, Sanjeev Kumar Tiwari, Kapil Lawrence* and Reena S. Lawrence ... Canola oil, animal fat, palm oil, corn oil, waste cooking oil (Felizardo et al., 2006; Kulkarni and Dalai, 2006) and Jatropha oil (Barnwal and Sharma, 2005) are other sources of commercial ...
The response to Cu 2+ exposure (10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on fifteen days post-em... more The response to Cu 2+ exposure (10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on fifteen days post-emergent Withania somnifera L. Dunal growing under natural environment in pots vis a vis Cu accumulation and antioxidative activity was studied on leaves, stems and roots. The duration of the experiment was for 2 months (Mid August-October, 2013) and interval between successive exposures was 7 days. As compared to control the following results were obtained. Copper (Cu 2+ ) content in plant tissues increased markedly with increasing soil Cu 2+ concentrations, and was highest in roots. Translocation factor (TF) and Bioconcentration Factor (BCF) values calculated were < 1, indicating a limited ability to accumulate and translocate Cu 2+ by the plant. Hydrogen peroxide formation was highest in roots and displayed a progressive increase in response to Cu 2+ exposure. With increasing Cu 2+ proline (non-enzymatic antioxidant) content was highest in leaves however, proline accumulation followe...
The effects of Cu +2 stress (0, 10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on growth and biochemic... more The effects of Cu +2 stress (0, 10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on growth and biochemical parameters of Withania somnifera L.Dunal, grown in a field pot experiment were studied. Increasing Cu +2 concentrations led to decreased stem length, root elongation and leaf area. Copper (Cu +2 ) stress decreased chlorophyll and carotenoids content in leaves and stems as compared to controls; this was more pronounced in leaves than in stems. Lipid peroxidation, in terms of malondialdehyde (MDA) content increased. Lipid peroxidation increased significantly with elevation in Cu +2 concentrations and was highest in roots followed by leaves and stems at 200 mM. Leakage of electrolytes measured in terms of membrane stability index (MSI), which showed a negative correlation with increasing Cu +2 levels. The MSI was lowest in 200 mM Cu +2 treated plants. The phenol content of leaves, stems and roots peaked at 50 mM Cu +2 treatment and thereafter declined. These results indicate that Cu + 2 ...
The present work is designed to evaluate the antioxidant, antibacterial activity and (TPC) total ... more The present work is designed to evaluate the antioxidant, antibacterial activity and (TPC) total phenolic content of Tagetes erecta flowers extracts. Antioxidant potential of flowers extracts was examined by applying three different methods. Antibacterial test was determined against gram positive and gram negative bacterium. The ethyl acetate extract had the greater phenolic concentration of 48.2 mg/g GAE followed by methanol extract which was 42.5 mg/g GAE and hexane extract 36.4 mg/g GAE. The ethyl acetate extract was found to be most effective in all the antioxidant methods in correlation with the TPC value. Moreover, all the flowers extracts are significantly effective in both Gram negative and Gram positive bacterium. Tagetes erecta flowers are hopefully considered as a promising source of natural pigments with a scope towards the alternative therapy for treatment of various health problems.
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