In this study, viability of an environmentally relevant bacterium, Escherichia coli exposed to 0-... more In this study, viability of an environmentally relevant bacterium, Escherichia coli exposed to 0-100 microg/mL chromium oxide nanoparticles (Cr2O3, Nps) for 120 min in Luria Bertani broth was evaluated by Propidium monoazide (PMA) assisted Q-PCR and standard plate count (SPC) method. Viable count for E. coli grown in Cr2O3, Nps amended medium was more by PMA assisted Q-PCR than SPC. Thus, the observations made in this study suggest that the inclusion of PMA assisted Q-PCR for viability assessment in Nps toxicity studies will provide the real count for the viable cells comprising of both viable and viable but not culturable (VBNC) cells.
Abstract:
Antimicrobials are widely used for the treatment of prevailing and emerging microbial d... more Abstract: Antimicrobials are widely used for the treatment of prevailing and emerging microbial diseases. Due to overuse of antibiotics, pathogens are developing multidrug resistance to them and causing adverse side effects. This has created a selection pressure to search for a new generation of antimicrobial agents. Nontraditional antimicrobial agents have been of tremendous interest in overcoming this problem of multidrug resistance developed by several pathogenic microorganisms against most of the commonly used antibiotics. These antimicrobial agents must be effective, safe and can be used for the cure of multidrug-resistant microbial infections. In recent times a lot many properties have been identified in metallic nanoparticles. They can offer effective solutions for these challenges. Several classes of antimicrobial nanoparticles (NPs) have proven their effectiveness for treating antibiotics resistant infectious diseases. This review summarizes emerging efforts in combating against infectious diseases, particularly using antimicrobial NPs as new tools to tackle the current challenges in treating infectious diseases. Key words: Multi drug resistance, Antibiotic resistance, Metallic Nanoparticles.
In this study, viability of an environmentally relevant bacterium, Escherichia coli exposed to 0-... more In this study, viability of an environmentally relevant bacterium, Escherichia coli exposed to 0-100 microg/mL chromium oxide nanoparticles (Cr2O3, Nps) for 120 min in Luria Bertani broth was evaluated by Propidium monoazide (PMA) assisted Q-PCR and standard plate count (SPC) method. Viable count for E. coli grown in Cr2O3, Nps amended medium was more by PMA assisted Q-PCR than SPC. Thus, the observations made in this study suggest that the inclusion of PMA assisted Q-PCR for viability assessment in Nps toxicity studies will provide the real count for the viable cells comprising of both viable and viable but not culturable (VBNC) cells.
Abstract:
Antimicrobials are widely used for the treatment of prevailing and emerging microbial d... more Abstract: Antimicrobials are widely used for the treatment of prevailing and emerging microbial diseases. Due to overuse of antibiotics, pathogens are developing multidrug resistance to them and causing adverse side effects. This has created a selection pressure to search for a new generation of antimicrobial agents. Nontraditional antimicrobial agents have been of tremendous interest in overcoming this problem of multidrug resistance developed by several pathogenic microorganisms against most of the commonly used antibiotics. These antimicrobial agents must be effective, safe and can be used for the cure of multidrug-resistant microbial infections. In recent times a lot many properties have been identified in metallic nanoparticles. They can offer effective solutions for these challenges. Several classes of antimicrobial nanoparticles (NPs) have proven their effectiveness for treating antibiotics resistant infectious diseases. This review summarizes emerging efforts in combating against infectious diseases, particularly using antimicrobial NPs as new tools to tackle the current challenges in treating infectious diseases. Key words: Multi drug resistance, Antibiotic resistance, Metallic Nanoparticles.
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Papers by Anurag Jyoti
Antimicrobials are widely used for the treatment of prevailing and emerging microbial diseases. Due to
overuse of antibiotics, pathogens are developing multidrug resistance to them and causing adverse side effects. This
has created a selection pressure to search for a new generation of antimicrobial agents. Nontraditional antimicrobial
agents have been of tremendous interest in overcoming this problem of multidrug resistance developed by several
pathogenic microorganisms against most of the commonly used antibiotics. These antimicrobial agents must be
effective, safe and can be used for the cure of multidrug-resistant microbial infections. In recent times a lot many
properties have been identified in metallic nanoparticles. They can offer effective solutions for these challenges.
Several classes of antimicrobial nanoparticles (NPs) have proven their effectiveness for treating antibiotics resistant
infectious diseases. This review summarizes emerging efforts in combating against infectious diseases, particularly
using antimicrobial NPs as new tools to tackle the current challenges in treating infectious diseases.
Key words: Multi drug resistance, Antibiotic resistance, Metallic Nanoparticles.
Antimicrobials are widely used for the treatment of prevailing and emerging microbial diseases. Due to
overuse of antibiotics, pathogens are developing multidrug resistance to them and causing adverse side effects. This
has created a selection pressure to search for a new generation of antimicrobial agents. Nontraditional antimicrobial
agents have been of tremendous interest in overcoming this problem of multidrug resistance developed by several
pathogenic microorganisms against most of the commonly used antibiotics. These antimicrobial agents must be
effective, safe and can be used for the cure of multidrug-resistant microbial infections. In recent times a lot many
properties have been identified in metallic nanoparticles. They can offer effective solutions for these challenges.
Several classes of antimicrobial nanoparticles (NPs) have proven their effectiveness for treating antibiotics resistant
infectious diseases. This review summarizes emerging efforts in combating against infectious diseases, particularly
using antimicrobial NPs as new tools to tackle the current challenges in treating infectious diseases.
Key words: Multi drug resistance, Antibiotic resistance, Metallic Nanoparticles.