Objectives: to evaluate the association between miRNAs and veno-occlusive erectile dysfunction.Re... more Objectives: to evaluate the association between miRNAs and veno-occlusive erectile dysfunction.Recently, this association between miRNAs and erectile dysfunction was extensively studied using animal models. Our aim was to explore the miRNAs expressions and functions in the development of erectile dysfunction, especially veno-occlusive dysfunction using a human tissue. Patients and methods: we prospectively recruited 60 patients with erectile dysfunction and controls between July 2015 and July 2016. The 30 patients were suffering from refractory veno-occlusive erectile dysfunction that was proven by investigations. They were scheduled for penile implant. The 30 controls were scheduled for repair of their fracture. We measured miRNAs (200a & 206) and nitric oxide (NO) in cavernous tissue and serum of both patients with erectile dysfunction and controls. Results: a significant association was found between the two mentioned miRNAs and erectile dysfunction (p < 0.001). Mean level of nitric oxide (NO) in cavernous tissue of the controls was significantly higher than that in the patients (p < 0.001). miRNA 200a showed a cutoff value of 1.135 with 95% sensitivity and 100% specificity while miRNA 206 showed a cutoff value of 1.125 with 100% sensitivity and 100% specificity. Conclusions: to the best of our knowledge, our study is the first report to measure the level of miRNAs in the cavernous tissue using a human tissue. Furthermore, this study can be considered a good step of deploying miRNAs through a blood test to detect early negative changes that lead to erectile dysfunction. Finally, we recommend more studies to be conducted to better understand if these miRNAs are involved in the pathophysiology of veno-occlusive erectile dysfunction.
Objectives: to evaluate the association between miRNAs and veno-occlusive erectile dysfunction.Re... more Objectives: to evaluate the association between miRNAs and veno-occlusive erectile dysfunction.Recently, this association between miRNAs and erectile dysfunction was extensively studied using animal models. Our aim was to explore the miRNAs expressions and functions in the development of erectile dysfunction, especially veno-occlusive dysfunction using a human tissue. Patients and methods: we prospectively recruited 60 patients with erectile dysfunction and controls between July 2015 and July 2016. The 30 patients were suffering from refractory veno-occlusive erectile dysfunction that was proven by investigations. They were scheduled for penile implant. The 30 controls were scheduled for repair of their fracture. We measured miRNAs (200a & 206) and nitric oxide (NO) in cavernous tissue and serum of both patients with erectile dysfunction and controls. Results: a significant association was found between the two mentioned miRNAs and erectile dysfunction (p < 0.001). Mean level of nitric oxide (NO) in cavernous tissue of the controls was significantly higher than that in the patients (p < 0.001). miRNA 200a showed a cutoff value of 1.135 with 95% sensitivity and 100% specificity while miRNA 206 showed a cutoff value of 1.125 with 100% sensitivity and 100% specificity. Conclusions: to the best of our knowledge, our study is the first report to measure the level of miRNAs in the cavernous tissue using a human tissue. Furthermore, this study can be considered a good step of deploying miRNAs through a blood test to detect early negative changes that lead to erectile dysfunction. Finally, we recommend more studies to be conducted to better understand if these miRNAs are involved in the pathophysiology of veno-occlusive erectile dysfunction.
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Papers by Hany Alghobary
Patients and methods: we prospectively recruited 60 patients with erectile dysfunction and controls between July 2015 and July 2016. The 30 patients were suffering from refractory veno-occlusive erectile dysfunction that was proven by investigations. They were scheduled for penile implant. The 30 controls were scheduled for repair of their fracture. We measured miRNAs (200a & 206) and nitric oxide (NO) in cavernous tissue and serum of both patients with erectile dysfunction and controls.
Results: a significant association was found between the two mentioned miRNAs and erectile dysfunction (p < 0.001). Mean level of nitric oxide (NO) in cavernous tissue of the controls was significantly higher than that in the patients (p < 0.001). miRNA 200a showed a cutoff value of 1.135 with 95% sensitivity and 100% specificity while miRNA 206 showed a cutoff value of 1.125 with 100% sensitivity and 100% specificity.
Conclusions: to the best of our knowledge, our study is the first report to measure the level of miRNAs in the cavernous tissue using a human tissue. Furthermore, this study can be considered a good step of deploying miRNAs through a blood test to detect early negative changes that lead to erectile dysfunction. Finally, we recommend more studies to be conducted to better understand if these miRNAs are involved in the pathophysiology of veno-occlusive erectile dysfunction.
Patients and methods: we prospectively recruited 60 patients with erectile dysfunction and controls between July 2015 and July 2016. The 30 patients were suffering from refractory veno-occlusive erectile dysfunction that was proven by investigations. They were scheduled for penile implant. The 30 controls were scheduled for repair of their fracture. We measured miRNAs (200a & 206) and nitric oxide (NO) in cavernous tissue and serum of both patients with erectile dysfunction and controls.
Results: a significant association was found between the two mentioned miRNAs and erectile dysfunction (p < 0.001). Mean level of nitric oxide (NO) in cavernous tissue of the controls was significantly higher than that in the patients (p < 0.001). miRNA 200a showed a cutoff value of 1.135 with 95% sensitivity and 100% specificity while miRNA 206 showed a cutoff value of 1.125 with 100% sensitivity and 100% specificity.
Conclusions: to the best of our knowledge, our study is the first report to measure the level of miRNAs in the cavernous tissue using a human tissue. Furthermore, this study can be considered a good step of deploying miRNAs through a blood test to detect early negative changes that lead to erectile dysfunction. Finally, we recommend more studies to be conducted to better understand if these miRNAs are involved in the pathophysiology of veno-occlusive erectile dysfunction.