La presente invención se refiere a un método para la detección de la bacteria Tropheryma whipplei... more La presente invención se refiere a un método para la detección de la bacteria Tropheryma whipplei en una muestra biológica aislada mediante la amplificación con pares de cebadores específicos de uno o más fragmentos de las secuencias nucleotídicas SEQ ID NO: 1, SEQ ID NO: 2 y SEQ ID NO: 3, donde dichas secuencias corresponden, respectivamente, a secuencias parciales de la secuencia nucleotídica del gen que codifica para la proteína HSP (Heat shock protein) de 65 kDa, de la secuencia nucleotídica de NCDRC (Non coding degenerated repeat cluster) y de la secuencia nucleotídica del gen que codifica para una proteína de la familia WISP (WNT1 inducible signaling pathway) de Tropheryma whipplei. Preferiblemente la amplificación se lleva a cabo por medio de PCR multiplex.; La detección de los productos de la amplificación se lleva a cabo por medio de sondas específicas complementarias a dichos productos. Asimismo, la presente invención se refiere a un kit que comprende los cebadores y sonda...
Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Fr... more Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella -like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella -like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida -like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents.
In order to study which Bartonella genotypes are circulating among small mammals in Spain, we ana... more In order to study which Bartonella genotypes are circulating among small mammals in Spain, we analyzed the spleens of 395 animals from three different areas—247 animals from the Basque Country (northern Spain), 121 animals from Catalonia (northeastern Spain), and 27 animals from Madrid (central Spain)—by a triplex PCR combined with a reverse line blot previously described by our group. The prevalence of Bartonella was 26.8% (106/395), and in 4.8% (19/395) of the animals more than one Bartonella genotype was detected. The study of gltA and the intergenic transcribed spacer in the positive samples demonstrated a large diversity, allowing the assignation of them into 22 genotypes. The most prevalent genotypes were 2 and 3, which are closely related to Bartonella taylorii . In addition, nine genotypes were associated with specific mammal species. Genotypes close to the zoonotic Bartonella grahamii , Bartonella elizabethae , and Bartonella rochalimae were also detected. Ten genotypes sho...
The development of high-Z (high atomic number) radiation shielding materials is vital in order to... more The development of high-Z (high atomic number) radiation shielding materials is vital in order to protect personnel who work with harmful gamma radiation sources. At the same time, the emission of external bremsstrahlung (EB) radiation in those shielding materials when the radiation source emits beta particles as well as gamma radiation is also of prime concern.The production of EB in films of lead monoxide (PbO) loaded polycarbonate (PC) composite at eleven different filler levels (FLs) varying, in terms of weight fraction, from 0.0 % up to 10.0 % were investigated experimentally by using beta particles from strontium-90/yttrium-90 (90Sr/90Y) radioactive source. A nonlinear relation is observed between EB intensity and target thickness. The effective atomic numbers of the prepared PbO-filled PC composite films (at different FLs) were determined via EB measurements, followed by calculations, and the values obtained were compared with the modified atomic numbers which were determined...
Atherosclerotic plaques and aneurysms are injuries associated with areas of low and oscillatory w... more Atherosclerotic plaques and aneurysms are injuries associated with areas of low and oscillatory wall shear stress at vascular bifurcations1. Experimental animal models have been used extensively in the development of new therapeutic approaches to this pathophysiological process.2 In ...
Although the defining feature of the vascular tree is its repeated bifurcation, only specific art... more Although the defining feature of the vascular tree is its repeated bifurcation, only specific arterial bifurcations are frequent sites for the development of obstructive atherosclerotic and aneurysmatic lesions.1 Despite the fact that several experimental approaches are available in which ...
The activities of Memphis hospitals to meet National Patient Safety Goals (NPSGs) for warfarin th... more The activities of Memphis hospitals to meet National Patient Safety Goals (NPSGs) for warfarin therapy are described. In March 2008, leadership from the Mid-South College of Clinical Pharmacy (MSCCP), a local chapter of the American College of Clinical Pharmacy, commissioned a task force on anticoagulation, comprising pharmacy administrators, clinical pharmacy practitioners, and pharmacy faculty from local hospitals within the greater Memphis area. The charge of the task force was to (1) identify practice variations in regard to NPSG.03.05.01, (2) develop professional collaboration among both academic and nonacademic institutions to share policy and protocol development, and (3) facilitate all institutions in meeting the deadlines set forth by the Joint Commission. The MSCCP Task Force on Anticoagulation project was successful in promoting collaboration among multiple institutions and clinical practitioners in the Memphis area. There was no one-size-fits-all approach; however, meetings and discussions were beneficial and led to idea generation. Having input from multiple institutions in different clinical settings with varying levels of experience created a rich environment from which all institutions benefited. For example, smaller institutions felt that they drew support for physician acceptance with protocol approval based on the knowledge of the policies approved or lessons learned at larger institutions. In addition, the larger institutions felt that the working group was helpful in validating their interpretation of the NPSG elements. The MSCCP Task Force on Anticoagulation project was successful in promoting collaboration among multiple institutions and clinical practitioners to offer solutions to meet NPSG.03.05.01 as it related to the needs of each institution.
La presente invención se refiere a un método para la detección de la bacteria Tropheryma whipplei... more La presente invención se refiere a un método para la detección de la bacteria Tropheryma whipplei en una muestra biológica aislada mediante la amplificación con pares de cebadores específicos de uno o más fragmentos de las secuencias nucleotídicas SEQ ID NO: 1, SEQ ID NO: 2 y SEQ ID NO: 3, donde dichas secuencias corresponden, respectivamente, a secuencias parciales de la secuencia nucleotídica del gen que codifica para la proteína HSP (Heat shock protein) de 65 kDa, de la secuencia nucleotídica de NCDRC (Non coding degenerated repeat cluster) y de la secuencia nucleotídica del gen que codifica para una proteína de la familia WISP (WNT1 inducible signaling pathway) de Tropheryma whipplei. Preferiblemente la amplificación se lleva a cabo por medio de PCR multiplex.; La detección de los productos de la amplificación se lleva a cabo por medio de sondas específicas complementarias a dichos productos. Asimismo, la presente invención se refiere a un kit que comprende los cebadores y sonda...
Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Fr... more Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella -like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella -like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida -like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents.
In order to study which Bartonella genotypes are circulating among small mammals in Spain, we ana... more In order to study which Bartonella genotypes are circulating among small mammals in Spain, we analyzed the spleens of 395 animals from three different areas—247 animals from the Basque Country (northern Spain), 121 animals from Catalonia (northeastern Spain), and 27 animals from Madrid (central Spain)—by a triplex PCR combined with a reverse line blot previously described by our group. The prevalence of Bartonella was 26.8% (106/395), and in 4.8% (19/395) of the animals more than one Bartonella genotype was detected. The study of gltA and the intergenic transcribed spacer in the positive samples demonstrated a large diversity, allowing the assignation of them into 22 genotypes. The most prevalent genotypes were 2 and 3, which are closely related to Bartonella taylorii . In addition, nine genotypes were associated with specific mammal species. Genotypes close to the zoonotic Bartonella grahamii , Bartonella elizabethae , and Bartonella rochalimae were also detected. Ten genotypes sho...
The development of high-Z (high atomic number) radiation shielding materials is vital in order to... more The development of high-Z (high atomic number) radiation shielding materials is vital in order to protect personnel who work with harmful gamma radiation sources. At the same time, the emission of external bremsstrahlung (EB) radiation in those shielding materials when the radiation source emits beta particles as well as gamma radiation is also of prime concern.The production of EB in films of lead monoxide (PbO) loaded polycarbonate (PC) composite at eleven different filler levels (FLs) varying, in terms of weight fraction, from 0.0 % up to 10.0 % were investigated experimentally by using beta particles from strontium-90/yttrium-90 (90Sr/90Y) radioactive source. A nonlinear relation is observed between EB intensity and target thickness. The effective atomic numbers of the prepared PbO-filled PC composite films (at different FLs) were determined via EB measurements, followed by calculations, and the values obtained were compared with the modified atomic numbers which were determined...
Atherosclerotic plaques and aneurysms are injuries associated with areas of low and oscillatory w... more Atherosclerotic plaques and aneurysms are injuries associated with areas of low and oscillatory wall shear stress at vascular bifurcations1. Experimental animal models have been used extensively in the development of new therapeutic approaches to this pathophysiological process.2 In ...
Although the defining feature of the vascular tree is its repeated bifurcation, only specific art... more Although the defining feature of the vascular tree is its repeated bifurcation, only specific arterial bifurcations are frequent sites for the development of obstructive atherosclerotic and aneurysmatic lesions.1 Despite the fact that several experimental approaches are available in which ...
The activities of Memphis hospitals to meet National Patient Safety Goals (NPSGs) for warfarin th... more The activities of Memphis hospitals to meet National Patient Safety Goals (NPSGs) for warfarin therapy are described. In March 2008, leadership from the Mid-South College of Clinical Pharmacy (MSCCP), a local chapter of the American College of Clinical Pharmacy, commissioned a task force on anticoagulation, comprising pharmacy administrators, clinical pharmacy practitioners, and pharmacy faculty from local hospitals within the greater Memphis area. The charge of the task force was to (1) identify practice variations in regard to NPSG.03.05.01, (2) develop professional collaboration among both academic and nonacademic institutions to share policy and protocol development, and (3) facilitate all institutions in meeting the deadlines set forth by the Joint Commission. The MSCCP Task Force on Anticoagulation project was successful in promoting collaboration among multiple institutions and clinical practitioners in the Memphis area. There was no one-size-fits-all approach; however, meetings and discussions were beneficial and led to idea generation. Having input from multiple institutions in different clinical settings with varying levels of experience created a rich environment from which all institutions benefited. For example, smaller institutions felt that they drew support for physician acceptance with protocol approval based on the knowledge of the policies approved or lessons learned at larger institutions. In addition, the larger institutions felt that the working group was helpful in validating their interpretation of the NPSG elements. The MSCCP Task Force on Anticoagulation project was successful in promoting collaboration among multiple institutions and clinical practitioners to offer solutions to meet NPSG.03.05.01 as it related to the needs of each institution.
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