Culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (AAGNC)... more Culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (AAGNC), may constitute a predominant group of organisms in oral sites. This study aimed to characterize phylogenetically 10 AAGNC isolated from endodontic lesions and periodontal pockets. 16S rDNA sequence and G + C content were determined. Strains sharing more than 98% sequence similarities and similar G + C content were considered the same bacterial species. One isolate resembled Dialister pneumosintes (the type species of the genus Dialister) with 35 mol% G + C content and 97% sequence similarity. Of eight isolates having 45-47 mol% G + C content, seven were identified as D. invisus and one resembled Dialister invisus with 97% sequence similarity. However the 16S rDNA sequence similarities with D. pneumosintes were relatively low, indicating the strains may belong to a new genus. The last isolate revealed 35 mol% G + C content, but had higher 16S rDNA sequence similarity with D. invisus than with D. pneumosintes. The group of oral AAGNC isolates need to be reclassified.
Background: As the most frequent infectious disease among children worldwide, dental caries have ... more Background: As the most frequent infectious disease among children worldwide, dental caries have a strong relationship with oral hygiene status, specifically in the development of infection. However, the study regarding the identification and distribution of oral Veillonella are limited. The oral Veillonella community may affected by the differences in geographical location, age, diet, lifestyle, socio-economic status and oral hygiene status. Here, we studied the oral hygiene status by examining the composition and proportion of oral Veillonella species in saliva of Japanese children. Methods: Microbial samples collected from 15 Japanese children divided into three oral hygiene groups were cultured under anaerobic conditions after homogenization and dilution, and inoculated onto brain heart infusion and selective medium Veillonella agar. Genomic DNA was extracted from each isolate. Veillonella species were detected by one-step PCR using rpoB species-specific primers. To analyse the ...
Secondary caries always occurs as a result of the filling not being hermetically. Purposes of thi... more Secondary caries always occurs as a result of the filling not being hermetically. Purposes of this research is to prove whether there is a leak on the border of the tooth enamel and border between the Glass-ionomer filling with the Streptococcus mutans infection with parameter of SMAAPPI ( Simplified S. mutans Approximal Plaque Index by Keeni et al, 1981). The subject of the research were 20 patients who came to the Dental Clinic at University of Indonesia with criteria possessing Glass-ionomer filling at the lower jaws. Collection of the samples were dental plaque gathered using a 1.5 mm excavator to scrape one way direction from the enamel, along the border between the enamel and Glass-ionomer filling and Glass-ionomer filling's surface. Isolation with medium transport sem-synthetic Cariostat and TSY20B and identification by using biochemical test. isolated colony strain local Streptococcus mutans from enamel, the border enamel and Glass-ionomer and the surface of the Glass-io...
National survey of dental health in 1995 indicated a moderate incidence of the disease among all ... more National survey of dental health in 1995 indicated a moderate incidence of the disease among all age groups. In order to increase the dental health status, a comparison of interest both military and civilian prevalence dengal caries was undueted. In 1996, a random sample of 258 officer, active duty, TNI-naval personnel were undertaken for monitoring the prevalence dental decay and severity of dental caries. Results show that the average DMF-T rate was 2.44 and 3.12 for both military and civilian personnel. The prevalence dental decay was 51.94% and had a significant at p < 0.001. The results show that dental health status of military personnel was better than civilian, besides the moderate incidence of dental caries.
The aim of the study was to evaluate the level of distribution of mutans streptococci on the marg... more The aim of the study was to evaluate the level of distribution of mutans streptococci on the margin of amalgam restoration, compared with that on the enamel and on the surface of restoration. It is assumed that the level of distribution of mutans streptococci on the margin will be able to influence the presence of secondary caries. In this study the first molars of 20 patients were treated with amalgam restoration. The mutans streptococci was measured as the percentage of total CFU count in the plaque. The results showed a mean count of 108.8 (SD= 55.2) of mutans streptococci in the margin; 97.7 (SD=63.5) on the enamel; and 61.4 (SD=32.4) on the surface of amalgam restoration. It seems that the level of mutans streptococci on the margin is higher than those on the enamel (p=0.006) as well as higher than those on the surface of (p=0.031). It is suggested that elevated level of mutans streptococci on the margin can indicate risk to secondary caries.
Veillonella spp., Gram-negative obligate anaerobic cocci bacteria, amounts to 3% in the oral cavi... more Veillonella spp., Gram-negative obligate anaerobic cocci bacteria, amounts to 3% in the oral cavity, relies on the fermentation of lactate as a carbon and energy source for growth. The bacteria are considered anti-cariogenic as they metabolize lactic acid into propionic acid which increases oral environment’s pH and reduces demineralization rate of tooth structure. Identification of Veillonella spp. using traditional methods is difficult due to the lack of conventional phenotypic and biochemical tests. Thus, the biomolecular methods are suitable for the specific detection and identification of Veillonella spp. One of the biomolecular methods that can be used is real-time Polymerase Chain Reaction (PCR), which the results can be qualitative and quantitative. This study aimed to identify Veillonella spp. in tongue plaque’s and saliva’s samples using Real -time PCR. The DNA of Veillonella spp. derived from 36 samples, 18 samples of tongue plaque and 18 samples of saliva, were extracted...
Damage on the primary teeth can be used as the best predictor of permanent tooth damage. ECC is a... more Damage on the primary teeth can be used as the best predictor of permanent tooth damage. ECC is a serious societal health problem in developing and industrial countries and can begin in the early stage of life and develop rapidly in children with high caries risk. The aim of this study was to analyze biological factors in 2-3 years old children in determining risk factors for early childhood caries. We conducted a cross-sectional study with samples of children, aged 2–3 years, who lived in selected villages with active Integrated Service Post in the Bandung City Community Health Center working area. Caries examination was performed as baseline data using the WHO standard check form to get information dmfs and oral hygiene was assessed using the Oral Hygiene Index Simplified (OHI-S). Microbiological examination was done to calculate the number of colonies of bacteria Streptococcus mutans on colony forming units (CFU). We performed statistical testing using the Chi-square test, Mann-W...
The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA... more The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA expression and cloning cDNA. This method consists of two parts: cDNA synthesis from mRNA by reverse transcription (RT) and amplification of cDNA by PCR. The RT-PCR has proved useful in amplifying very small amount of specific mRNA to obtain cDNA clones and to detect mRNA transcripts from abnormal cells in a background of normal cells.
Asian Journal of Pharmaceutical and Clinical Research, 2017
Objective: This study aimed to compare the effect of Curcuma xanthrorrhiza ethanol extract to the... more Objective: This study aimed to compare the effect of Curcuma xanthrorrhiza ethanol extract to the viability of Streptococcus mutans and Aggregatibacter actinomycetemcomitans using single- and dual-species biofilm at different phases of formation. Methods: Biofilm models were incubated for 4, 12, and 24 hrs, then exposed to the extract at a concentration of 0.525%. Results: The viability of the single-species S. mutans biofilm was low (p 0.05) was found between singlespecies A. actinomycetemcomitans and dual-species biofilm. Conclusions: Curcuma xanthorrhiza ethanol extract is more effective for decreasing the viability of single-species S. mutans biofilm.
Journal of International Dental and Medical Research, 2017
The presence of oral Veillonela spp., V. atypica, V. denticariosi, V. dispar, V. parvula and V. r... more The presence of oral Veillonela spp., V. atypica, V. denticariosi, V. dispar, V. parvula and V. rogosae, in oral mucosa biofilms and saliva have been associated with dental cary development in young children. However, identifying oral Veillonella spp. is difficult since there is no useful differential phenotypic test. To reliably detect Veillonellarelated early childhood caries (ECC), we used a real-time PCR assay based on SYBR Green I, followed by a melting curve analysis. The q-PCR identified the oral Veillonella spp. directly from the clinical specimen. The oral mucosa biofilm collected from ECC was dominated by V. dispar, while the minor species was V. rogosae. This is the first report indicating the profile of oral Veillonella spp. in the oral mucosa biofilm of high-risk EEC in Indonesian subjects. Experimental article (J Int Dent Med Res 2017; 10(Special Issue): pp. 757-763)
The prevalence of denture stomatitis is high in denture wearers. The objective of this study was ... more The prevalence of denture stomatitis is high in denture wearers. The objective of this study was to measure the effect of the surface roughness of denture base materials on the amount of Streptococcus mutans present. The surface roughness was measured using a surface roughness tester. The specimens were dipped into an Eppendorf tube containing Streptococcus mutans and incubated for 12 and 24 hours. A bivariate Pearson correlation test was performed on the data. There is a strong positive correlation between the surface roughness of the denture base material and the amount of Streptococcus mutans. A decrease in the value of the surface roughness after polishing a metal, acrylic resin, or valplast denture base is followed by a decrease in the amount of Streptococcus mutans. Experimental article (J Int Dent Med Res 2018; 11(3): 899-905)
Culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (AAGNC)... more Culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (AAGNC), may constitute a predominant group of organisms in oral sites. This study aimed to characterize phylogenetically 10 AAGNC isolated from endodontic lesions and periodontal pockets. 16S rDNA sequence and G + C content were determined. Strains sharing more than 98% sequence similarities and similar G + C content were considered the same bacterial species. One isolate resembled Dialister pneumosintes (the type species of the genus Dialister) with 35 mol% G + C content and 97% sequence similarity. Of eight isolates having 45-47 mol% G + C content, seven were identified as D. invisus and one resembled Dialister invisus with 97% sequence similarity. However the 16S rDNA sequence similarities with D. pneumosintes were relatively low, indicating the strains may belong to a new genus. The last isolate revealed 35 mol% G + C content, but had higher 16S rDNA sequence similarity with D. invisus than with D. pneumosintes. The group of oral AAGNC isolates need to be reclassified.
Background: As the most frequent infectious disease among children worldwide, dental caries have ... more Background: As the most frequent infectious disease among children worldwide, dental caries have a strong relationship with oral hygiene status, specifically in the development of infection. However, the study regarding the identification and distribution of oral Veillonella are limited. The oral Veillonella community may affected by the differences in geographical location, age, diet, lifestyle, socio-economic status and oral hygiene status. Here, we studied the oral hygiene status by examining the composition and proportion of oral Veillonella species in saliva of Japanese children. Methods: Microbial samples collected from 15 Japanese children divided into three oral hygiene groups were cultured under anaerobic conditions after homogenization and dilution, and inoculated onto brain heart infusion and selective medium Veillonella agar. Genomic DNA was extracted from each isolate. Veillonella species were detected by one-step PCR using rpoB species-specific primers. To analyse the ...
Secondary caries always occurs as a result of the filling not being hermetically. Purposes of thi... more Secondary caries always occurs as a result of the filling not being hermetically. Purposes of this research is to prove whether there is a leak on the border of the tooth enamel and border between the Glass-ionomer filling with the Streptococcus mutans infection with parameter of SMAAPPI ( Simplified S. mutans Approximal Plaque Index by Keeni et al, 1981). The subject of the research were 20 patients who came to the Dental Clinic at University of Indonesia with criteria possessing Glass-ionomer filling at the lower jaws. Collection of the samples were dental plaque gathered using a 1.5 mm excavator to scrape one way direction from the enamel, along the border between the enamel and Glass-ionomer filling and Glass-ionomer filling's surface. Isolation with medium transport sem-synthetic Cariostat and TSY20B and identification by using biochemical test. isolated colony strain local Streptococcus mutans from enamel, the border enamel and Glass-ionomer and the surface of the Glass-io...
National survey of dental health in 1995 indicated a moderate incidence of the disease among all ... more National survey of dental health in 1995 indicated a moderate incidence of the disease among all age groups. In order to increase the dental health status, a comparison of interest both military and civilian prevalence dengal caries was undueted. In 1996, a random sample of 258 officer, active duty, TNI-naval personnel were undertaken for monitoring the prevalence dental decay and severity of dental caries. Results show that the average DMF-T rate was 2.44 and 3.12 for both military and civilian personnel. The prevalence dental decay was 51.94% and had a significant at p < 0.001. The results show that dental health status of military personnel was better than civilian, besides the moderate incidence of dental caries.
The aim of the study was to evaluate the level of distribution of mutans streptococci on the marg... more The aim of the study was to evaluate the level of distribution of mutans streptococci on the margin of amalgam restoration, compared with that on the enamel and on the surface of restoration. It is assumed that the level of distribution of mutans streptococci on the margin will be able to influence the presence of secondary caries. In this study the first molars of 20 patients were treated with amalgam restoration. The mutans streptococci was measured as the percentage of total CFU count in the plaque. The results showed a mean count of 108.8 (SD= 55.2) of mutans streptococci in the margin; 97.7 (SD=63.5) on the enamel; and 61.4 (SD=32.4) on the surface of amalgam restoration. It seems that the level of mutans streptococci on the margin is higher than those on the enamel (p=0.006) as well as higher than those on the surface of (p=0.031). It is suggested that elevated level of mutans streptococci on the margin can indicate risk to secondary caries.
Veillonella spp., Gram-negative obligate anaerobic cocci bacteria, amounts to 3% in the oral cavi... more Veillonella spp., Gram-negative obligate anaerobic cocci bacteria, amounts to 3% in the oral cavity, relies on the fermentation of lactate as a carbon and energy source for growth. The bacteria are considered anti-cariogenic as they metabolize lactic acid into propionic acid which increases oral environment’s pH and reduces demineralization rate of tooth structure. Identification of Veillonella spp. using traditional methods is difficult due to the lack of conventional phenotypic and biochemical tests. Thus, the biomolecular methods are suitable for the specific detection and identification of Veillonella spp. One of the biomolecular methods that can be used is real-time Polymerase Chain Reaction (PCR), which the results can be qualitative and quantitative. This study aimed to identify Veillonella spp. in tongue plaque’s and saliva’s samples using Real -time PCR. The DNA of Veillonella spp. derived from 36 samples, 18 samples of tongue plaque and 18 samples of saliva, were extracted...
Damage on the primary teeth can be used as the best predictor of permanent tooth damage. ECC is a... more Damage on the primary teeth can be used as the best predictor of permanent tooth damage. ECC is a serious societal health problem in developing and industrial countries and can begin in the early stage of life and develop rapidly in children with high caries risk. The aim of this study was to analyze biological factors in 2-3 years old children in determining risk factors for early childhood caries. We conducted a cross-sectional study with samples of children, aged 2–3 years, who lived in selected villages with active Integrated Service Post in the Bandung City Community Health Center working area. Caries examination was performed as baseline data using the WHO standard check form to get information dmfs and oral hygiene was assessed using the Oral Hygiene Index Simplified (OHI-S). Microbiological examination was done to calculate the number of colonies of bacteria Streptococcus mutans on colony forming units (CFU). We performed statistical testing using the Chi-square test, Mann-W...
The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA... more The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA expression and cloning cDNA. This method consists of two parts: cDNA synthesis from mRNA by reverse transcription (RT) and amplification of cDNA by PCR. The RT-PCR has proved useful in amplifying very small amount of specific mRNA to obtain cDNA clones and to detect mRNA transcripts from abnormal cells in a background of normal cells.
Asian Journal of Pharmaceutical and Clinical Research, 2017
Objective: This study aimed to compare the effect of Curcuma xanthrorrhiza ethanol extract to the... more Objective: This study aimed to compare the effect of Curcuma xanthrorrhiza ethanol extract to the viability of Streptococcus mutans and Aggregatibacter actinomycetemcomitans using single- and dual-species biofilm at different phases of formation. Methods: Biofilm models were incubated for 4, 12, and 24 hrs, then exposed to the extract at a concentration of 0.525%. Results: The viability of the single-species S. mutans biofilm was low (p 0.05) was found between singlespecies A. actinomycetemcomitans and dual-species biofilm. Conclusions: Curcuma xanthorrhiza ethanol extract is more effective for decreasing the viability of single-species S. mutans biofilm.
Journal of International Dental and Medical Research, 2017
The presence of oral Veillonela spp., V. atypica, V. denticariosi, V. dispar, V. parvula and V. r... more The presence of oral Veillonela spp., V. atypica, V. denticariosi, V. dispar, V. parvula and V. rogosae, in oral mucosa biofilms and saliva have been associated with dental cary development in young children. However, identifying oral Veillonella spp. is difficult since there is no useful differential phenotypic test. To reliably detect Veillonellarelated early childhood caries (ECC), we used a real-time PCR assay based on SYBR Green I, followed by a melting curve analysis. The q-PCR identified the oral Veillonella spp. directly from the clinical specimen. The oral mucosa biofilm collected from ECC was dominated by V. dispar, while the minor species was V. rogosae. This is the first report indicating the profile of oral Veillonella spp. in the oral mucosa biofilm of high-risk EEC in Indonesian subjects. Experimental article (J Int Dent Med Res 2017; 10(Special Issue): pp. 757-763)
The prevalence of denture stomatitis is high in denture wearers. The objective of this study was ... more The prevalence of denture stomatitis is high in denture wearers. The objective of this study was to measure the effect of the surface roughness of denture base materials on the amount of Streptococcus mutans present. The surface roughness was measured using a surface roughness tester. The specimens were dipped into an Eppendorf tube containing Streptococcus mutans and incubated for 12 and 24 hours. A bivariate Pearson correlation test was performed on the data. There is a strong positive correlation between the surface roughness of the denture base material and the amount of Streptococcus mutans. A decrease in the value of the surface roughness after polishing a metal, acrylic resin, or valplast denture base is followed by a decrease in the amount of Streptococcus mutans. Experimental article (J Int Dent Med Res 2018; 11(3): 899-905)
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