In the present work we carried out a phytochemical and biological investigation on three endemic ... more In the present work we carried out a phytochemical and biological investigation on three endemic Hypericum species, i.e.Hypericum thymbrifolium (H. thymbrifolium), Hypericum spectabile (H. spectabile) and Hypericum pseudolaeve (H. pseudolaeve) from Anatolia in order to discover new sources of natural compounds for the treatment of inflammatory and neurodegenerative disorders. HPLC-DAD analysis indicated that two naphthodianthrones (pseudohypericin and hypericin) together with chlorogenic acid, rutin, hyperoside, isoquercitrin, kaempferol, quercitrin, quercetin, amentoflavone, and hyperforin are the main compounds present in the methanol extracts. After chemical characterization, all extracts were in-vitro biologically assayed for antioxidant potential by lipid peroxidation inhibitory activity, DPPH, FRAP assays, and superoxide radical scavenging activity, for AChE inhibitory activity by Ellman's method, for COX inhibitory activity by using enzyme immunoassay (EIA) kit, for cytotoxic activity on HeLa and NRK-52E cell lines by MTT assay. The superoxide radical scavenging activity and lipid peroxidation inhibitory activity of H. spectabile (EC50 = 0.430 mg/mL) were more remarkable than that of H. thymbrifolium and H. pseudolaeve. The extracts showed moderate inhibitory activity on AChE (from 49.37% to 63.41%). The best inhibitory activity against COX-1 (71.77% and 77.04%, respectively) and COX-2 ( 64.14% and 72.23%, respectively) were shown by H. thymbrifolium and H. spectabile, which may be due to their richest chlorogenic acid content (0.29576% and 0.23567%, respectively). Cytotoxicity screening results showed that the extracts did not demonstrate significant cytotoxic activity. It was concluded that the most promising extract with antioxidant, anti-inflammatory, and AChE inhibition potential is H. spectabile.
Nearly 350 Hypericum species exist on the earth, widely spreading in Europe, Asia and North Afric... more Nearly 350 Hypericum species exist on the earth, widely spreading in Europe, Asia and North Africa. 32 of 80 species growing in Turkey are endemic. The chemical composition of the essential oils obtained from the aerial parts of the H. uniglandulosum Hausskn. ex Bornm., H.scabroides Robson and Poulter, H.kotschyanum Boiss., H.salsugineum Robson and Hub.-Mor. and H.thymopsis Boiss. By using the hydro-distillation method is identified by GC and GC/ MS. Finally the results are compared with each other. The differences between the results of the H.thymopsis and H.scabroides obtained in this study and the previous studies show that the chemical compositions of the essential oils are different for the same species obtained at different locations. The essential oil compositions of these species, except for the H. thymopsis and H.scabroides are identified for the first time.
Abstract Zika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. In... more Abstract Zika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. Investigations to find more natural but highly effective pesticidal agents against Ae. aegypti have increased in the last few years. Plant-based compounds have been the source of several current pesticides and have provided models for creation of synthetic derivatives like the pyrethroids. Plants continue to be a rich source for potential new active ingredients. The aim of this study is to increase knowledge on V. dioscoridis and to present the results of the cytotoxic and insecticidal activities of various extracts of its roots and volatile compound compositions of roots. The hexane and chloroform extracts, HM1 and CM1 respectively, exhibited cytotoxicity with IC50 values of 128.4 and 86.93 µg/mL against the HepG2 cell line. Because CM1 had higher cytotoxicity across the studied concentration range, it was also tested against the HUVEC cell line. Extracts HM1 and CM1 were also tested for their insecticidal activities. CM1 was found to be the more active (mortality (%) = 96.7% ± 5.8 at 5 μg/mosquito), consistent with its cytotoxic activity. Since these active extracts were nonpolar, the chemical composition of the volatile compounds of the roots were analyzed by GC-MS analysis. About 87.7% of the essential oil were characterized. The major compound was s-eudesmol (9.2%), following by camphene (8.9%), bornyl acetate (8.2%) and maaliol (7.6%). This is the first study on the HepG2 and HUVEC cytotoxicity and insecticidal activity of V. dioscoridis. Further studies on the potential of this plant to define the spectrum of activity and examine potential therapeutic agents is warranted.
ABSTRACT The chemical composition of methanolic extract of Hypericum pamphylicum (HPM) was analyz... more ABSTRACT The chemical composition of methanolic extract of Hypericum pamphylicum (HPM) was analyzed by usinghigh performance liquid chromatography–diode array detector (HPLC-DAD). The plant contained hyper-icin (0.00002 ± 0.000001%), hyperforin (trace), chlorogenic acid (5.58 ± 1.59%), hyperoside (0.46 ± 0.05%),isoquercitrin (0.134 ± 0.02%) and quercetin (0.087 ± 0.008%). The antioxidant activity of HPM was eval-uated by using several antioxidant tests. HPM exhibits inhibitory capacity against phosphatidylcholineliposome peroxidation, induced with iron and ascorbic acid, scavenge DPPH and superoxide radicals andact as reductants. The cytotoxic activity of HPM was also determined by using MTT cell viability assay onHeLa and NRK-52E cell lines. HPM demonstrated no cytotoxicity against HeLa and NRK-52E cell lines withIC50values of 1.89 mg/ml and 1.05 mg/ml, respectively. The different crude extracts of H. pamphylicumwere analyzed using a microdilution assay for antimicrobial activity against several microorganisms. Themost active extract was HPM which showed activity against Staphylococcus aureus. It is observed thatnearly all extracts showed activity against Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumo-niae, Pseudomonas aeruginosa, Salmonella thypi, Shigella flexneri, Proteus mirabilis and Candida albicans.
Journal of Faculty Pharmacy of Istanbul University, 2007
Ege Bolgesinin 27 ilinde geleneksel olarak kullanilan tibbi bitkiler arastirilmistir. Halk ile ya... more Ege Bolgesinin 27 ilinde geleneksel olarak kullanilan tibbi bitkiler arastirilmistir. Halk ile yapilan gorusmeler sonucunda bilgiler derlenmis ve ornekler toplanmistir. Toplanan 106 adet tibbi bitki ornegi tayin edilerek yerel isimleri, kullanilan kisimlari, hazirlanma sekilleri ve tedavideki kullanilisi bildirilmistir.
In the present work we carried out a phytochemical and biological investigation on three endemic ... more In the present work we carried out a phytochemical and biological investigation on three endemic Hypericum species, i.e.Hypericum thymbrifolium (H. thymbrifolium), Hypericum spectabile (H. spectabile) and Hypericum pseudolaeve (H. pseudolaeve) from Anatolia in order to discover new sources of natural compounds for the treatment of inflammatory and neurodegenerative disorders. HPLC-DAD analysis indicated that two naphthodianthrones (pseudohypericin and hypericin) together with chlorogenic acid, rutin, hyperoside, isoquercitrin, kaempferol, quercitrin, quercetin, amentoflavone, and hyperforin are the main compounds present in the methanol extracts. After chemical characterization, all extracts were in-vitro biologically assayed for antioxidant potential by lipid peroxidation inhibitory activity, DPPH, FRAP assays, and superoxide radical scavenging activity, for AChE inhibitory activity by Ellman's method, for COX inhibitory activity by using enzyme immunoassay (EIA) kit, for cytotoxic activity on HeLa and NRK-52E cell lines by MTT assay. The superoxide radical scavenging activity and lipid peroxidation inhibitory activity of H. spectabile (EC50 = 0.430 mg/mL) were more remarkable than that of H. thymbrifolium and H. pseudolaeve. The extracts showed moderate inhibitory activity on AChE (from 49.37% to 63.41%). The best inhibitory activity against COX-1 (71.77% and 77.04%, respectively) and COX-2 ( 64.14% and 72.23%, respectively) were shown by H. thymbrifolium and H. spectabile, which may be due to their richest chlorogenic acid content (0.29576% and 0.23567%, respectively). Cytotoxicity screening results showed that the extracts did not demonstrate significant cytotoxic activity. It was concluded that the most promising extract with antioxidant, anti-inflammatory, and AChE inhibition potential is H. spectabile.
Nearly 350 Hypericum species exist on the earth, widely spreading in Europe, Asia and North Afric... more Nearly 350 Hypericum species exist on the earth, widely spreading in Europe, Asia and North Africa. 32 of 80 species growing in Turkey are endemic. The chemical composition of the essential oils obtained from the aerial parts of the H. uniglandulosum Hausskn. ex Bornm., H.scabroides Robson and Poulter, H.kotschyanum Boiss., H.salsugineum Robson and Hub.-Mor. and H.thymopsis Boiss. By using the hydro-distillation method is identified by GC and GC/ MS. Finally the results are compared with each other. The differences between the results of the H.thymopsis and H.scabroides obtained in this study and the previous studies show that the chemical compositions of the essential oils are different for the same species obtained at different locations. The essential oil compositions of these species, except for the H. thymopsis and H.scabroides are identified for the first time.
Abstract Zika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. In... more Abstract Zika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. Investigations to find more natural but highly effective pesticidal agents against Ae. aegypti have increased in the last few years. Plant-based compounds have been the source of several current pesticides and have provided models for creation of synthetic derivatives like the pyrethroids. Plants continue to be a rich source for potential new active ingredients. The aim of this study is to increase knowledge on V. dioscoridis and to present the results of the cytotoxic and insecticidal activities of various extracts of its roots and volatile compound compositions of roots. The hexane and chloroform extracts, HM1 and CM1 respectively, exhibited cytotoxicity with IC50 values of 128.4 and 86.93 µg/mL against the HepG2 cell line. Because CM1 had higher cytotoxicity across the studied concentration range, it was also tested against the HUVEC cell line. Extracts HM1 and CM1 were also tested for their insecticidal activities. CM1 was found to be the more active (mortality (%) = 96.7% ± 5.8 at 5 μg/mosquito), consistent with its cytotoxic activity. Since these active extracts were nonpolar, the chemical composition of the volatile compounds of the roots were analyzed by GC-MS analysis. About 87.7% of the essential oil were characterized. The major compound was s-eudesmol (9.2%), following by camphene (8.9%), bornyl acetate (8.2%) and maaliol (7.6%). This is the first study on the HepG2 and HUVEC cytotoxicity and insecticidal activity of V. dioscoridis. Further studies on the potential of this plant to define the spectrum of activity and examine potential therapeutic agents is warranted.
ABSTRACT The chemical composition of methanolic extract of Hypericum pamphylicum (HPM) was analyz... more ABSTRACT The chemical composition of methanolic extract of Hypericum pamphylicum (HPM) was analyzed by usinghigh performance liquid chromatography–diode array detector (HPLC-DAD). The plant contained hyper-icin (0.00002 ± 0.000001%), hyperforin (trace), chlorogenic acid (5.58 ± 1.59%), hyperoside (0.46 ± 0.05%),isoquercitrin (0.134 ± 0.02%) and quercetin (0.087 ± 0.008%). The antioxidant activity of HPM was eval-uated by using several antioxidant tests. HPM exhibits inhibitory capacity against phosphatidylcholineliposome peroxidation, induced with iron and ascorbic acid, scavenge DPPH and superoxide radicals andact as reductants. The cytotoxic activity of HPM was also determined by using MTT cell viability assay onHeLa and NRK-52E cell lines. HPM demonstrated no cytotoxicity against HeLa and NRK-52E cell lines withIC50values of 1.89 mg/ml and 1.05 mg/ml, respectively. The different crude extracts of H. pamphylicumwere analyzed using a microdilution assay for antimicrobial activity against several microorganisms. Themost active extract was HPM which showed activity against Staphylococcus aureus. It is observed thatnearly all extracts showed activity against Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumo-niae, Pseudomonas aeruginosa, Salmonella thypi, Shigella flexneri, Proteus mirabilis and Candida albicans.
Journal of Faculty Pharmacy of Istanbul University, 2007
Ege Bolgesinin 27 ilinde geleneksel olarak kullanilan tibbi bitkiler arastirilmistir. Halk ile ya... more Ege Bolgesinin 27 ilinde geleneksel olarak kullanilan tibbi bitkiler arastirilmistir. Halk ile yapilan gorusmeler sonucunda bilgiler derlenmis ve ornekler toplanmistir. Toplanan 106 adet tibbi bitki ornegi tayin edilerek yerel isimleri, kullanilan kisimlari, hazirlanma sekilleri ve tedavideki kullanilisi bildirilmistir.
Uploads
Papers