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Imaging a typical cell by soft X-ray tomography requires collection of projection images at 1-2° increments through 180° rotation. During this procedure, the specimen receives a dose of radiation large enough to cause a significant degree of structural damage.
Abstract. Cellular tomography using X-rays is a novel technique that allows acquisition of structural information of complete cells, in their native ...
Image processing for Cellular tomography using soft X-rays
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Oct 22, 2024 · Cellular tomography using X-rays is a novel technique that allows acquisition of structural information of complete cells, in their native ...
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Soft X-ray tomography (SXT) is a non-invasive, 3D imaging technique that can measure volumes, surfaces, interfaces, membranes, and organelle connectivity within ...
Nov 22, 2021 · We employ full-rotation soft X-ray tomography (SXT) to examine organelle remodeling induced by SARS-CoV-2 at the whole-cell level with high spatial resolution ...
Soft X-ray tomography (SXT) generates 3D organelle maps of intact cells. · 3D maps reveal the positions of mitochondria and their molecular densities. · SXT can ...
Cryo-soft X-ray tomography (cryo-SXT) is a powerful microscopy technique, which resolves the three-dimensional ultrastructure of cells in their near-native ...
Feb 10, 2022 · The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography.
Nov 29, 2024 · Soft X-ray tomography (SXT) allows for three-dimensional imaging of cellular structures while they remain in their natural, hydrated state, ...
Dec 15, 2022 · 2.1 Image formation using soft X-rays. Soft X-rays are most effective for imaging hydrated cells when used in an absorption contrast microscope.