The RING-CH Ligase K5 Antagonizes Restriction of KSHV and HIV-1 Particle Release by Mediating Ubiquitin-Dependent Endosomal Degradation of Tetherin
Figure 9
Cell-surface down-regulation of tetherin by K5 is inhibited by dominant-negative VPS4.
(A) HeLa cells were transfected with wild type K5 or K5(del RING) expression vectors in combination with either GFP or a dominant negative mutant of VPS4 fused to GFP (VPS4 E228Q). 24h later cells were stained for cell surface tetherin using anti-BST2 monoclonal antibody and a goat-anti-mouse Alexa633 secondary antibody. Flow cytometry dot plots are shown. (B) The mean florescent intensities (MFI) of surface tetherin expression in GFP positive and GFP negative populations from the samples in panel A are plotted.
doi: https://doi.org/10.1371/journal.ppat.1000843.g009