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14 July 1993 FEL microscopy for the investigation of transient local heating in single living cells
Bruce J. Tromberg, K. A. Peterson, Tatiana B. Krasieva, Satoshi Shimizu, Chris W. Rella, Andrew Gee Jeung, Curtis F. Chapman, Dana D. Dlott, Michael D. Fayer, H. Alan Schwettman, Michael W. Berns
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Proceedings Volume 1854, Free-Electron Laser Spectroscopy in Biology, Medicine, and Materials Science; (1993) https://doi.org/10.1117/12.148038
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
Abstract
A free-electron laser (FEL) microscope has been constructed to perform spatially and spectrally resolved pump/probe experiments in single living cells. Picosecond infrared FEL pulses are absorbed by the sample and rapidly converted to heat. Excitation of localized fluorescent reporter molecules using a UV/VIS probe beam leads to thermally induced alterations in the radiative signal. Fluorescence-detected infrared (FDIR) spectra are generated from regions proximal to reporter molecules by varying the FEL pump wavelength. Sub- wavelength spatial resolution is a composite function of media thermal properties and probe selectivity.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Bruce J. Tromberg, K. A. Peterson, Tatiana B. Krasieva, Satoshi Shimizu, Chris W. Rella, Andrew Gee Jeung, Curtis F. Chapman, Dana D. Dlott, Michael D. Fayer, H. Alan Schwettman, and Michael W. Berns "FEL microscopy for the investigation of transient local heating in single living cells", Proc. SPIE 1854, Free-Electron Laser Spectroscopy in Biology, Medicine, and Materials Science, (14 July 1993); https://doi.org/10.1117/12.148038
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KEYWORDS
Free electron lasers
Luminescence
Microscopes
Infrared radiation
FT-IR spectroscopy
Absorption
Microscopy
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