IBA Lifesciences

📅 We are looking forward to the annual PEGS Europe Summit next week in Barcelona and will be happy to see you during our talk and poster session, as well as welcome you at our booth (1507) at the exhibitor hall. As part of the Expression Stream, Day 1, Fabian Mohr, CSO, will present, how magnetic beads distinguished by high binding capacity and specificity can be flexibly applied in screenings, small- and large-scale purifications. During Poster Session A (Tuesday-Wednesday morning), Philipp Henning will introduce the topic of purification of in vivo biotinylated proteins backed up by our latest data. If you would like to schedule a meeting at our booth in advance, please reach out to Dr. Alexandra Nordlohne and Benedikt Ni. #PEGSummit #PEGS2024 #PEGSEurope

💡Surface plasmon resonance (SPR) is a powerful method to study biomolecular interactions. Due to its high accuracy and ability to measure samples in high throughput, it plays a major role in research and development of drugs. A crucial step in SPR analysis is the immobilization of the ligand to the surface of the biosensor chip. The immobilization of the ligand can take place via direct covalent coupling to the surface of the chip or by capture with the help of a pre-coated capture molecule. However, the direct covalent immobilization of the ligand can change its biological activity or result in an undirected immobilization with reduced accessibility of the binding sites. The transient immobilization of tagged ligands via affinity capturing molecules overcomes these drawbacks. ➡️ Click here for the comparison of the applicability of the two widely-used affinity tags for measuring binding kinetics via SPR: https://lnkd.in/dQEDe-Y7 #biotech #biotechnology #proteininteraction #proteinstudies #drugdiscovery

📝White Paper of the month Choosing an appropriate affinity chromatography system for simple and efficient protein purification is a common question. There are differences between the systems which rarely are clearly represented, making it hard for scientist to reach a decision. ➡️This comprehensive comparison of the most frequently used systems, His-tag and Strep-tag®, explains these differences as well as recommends one system depending on the properties of the target protein, expression host and purification conditions: https://lnkd.in/dB9AS-e6 #molecularbiology #proteinstudies #proteinpurification #affinitychromatography #biotechnology

Understanding protein-protein interactions (PPIs) is fundamental to the field of molecular biology and has broad impact in many areas. From the optimization of drug discovery leads to the identification of biomarkers for disease diagnosis and monitoring, PPIs play a critical role in advancing medical research and therapeutic development. ➡️ Read our detailed description and illustration of the key steps to identify and characterize the interaction between two specific proteins and learn how each of these steps can benefit from using Strep-tag® technology here: https://lnkd.in/dXURFcCA #biotech #biotechnology #proteinpurification #proteininteraction #proteinstudies #streptag #drugdiscovery

🔔📣 #wearehiring #lifesciencejobs #biotechjobs #Naturwissenschaft

Thanks to its high affinity, Strep-tag® technology is ideal for BLI kinetic analysis and SPR measurements. ➡️ Read more about these methods for measuring binding kinetics in the great summary below and on our homepage https://lnkd.in/dtJGdc9E

🧲 Magnetic beads are a versatile purification and protein-interaction analysis tool. Depending on the experimental conditions, it is possible to shorten the purification protocol and increase protein yield by adjusting specific parameters, such as - bead volume - incubation time - and protein concentration. ➡️ Click here for the corresponding tips, FAQs and data: https://lnkd.in/dAZkPR7Z #biotech #biotechnology #proteinpurification #affinitychromatography #streptag

📅 Eventful week ahead! Next week our team is attending BioTalk Conference in Berlin, as well as annual Horizons in Molecular Biology Symposium in our home city of Göttingen. We are looking forward to meeting you at our booth!

❓What are the differences between Strep-Tactin® and Strep-Tactin®XT? The two engineered Streptavidin variants, Strep-Tactin® and its high-affinity counterpart Strep-Tactin®XT, differ in their binding affinities towards Strep-tag® and Twin-Strep-tag® peptides, as well as usability in different analytical applications, protein purification efficiency, yield, costs per mg of protein, and resin stability. ➡️ Learn more about the differences between the two ligands here: https://lnkd.in/dRjAptMW #biotech #biotechnology #proteinpurification #affinitychromatography #streptag

❓ What are the different types of magnetic beads offered by IBA and what is their intended use? 🧲Magnetic beads are one of the most versatile protein purification tools. The magnetic core allows them to be separated from the supernatant with a magnet. This eliminates centrifugation steps and enables quick and easy handling during batch purification. Besides batch protein purification, magnetic beads can be used for interaction studies via immunoprecipitation, BioID or Pull-Down assays, in high-throughput applications, or even for cell isolation. We currently have 3 different types of magnetic beads in our product portfolio: 🔹MagStrep® Strep-Tactin®XT beads – Purification of Strep-tag®II and Twin-Strep-tag® fusion proteins, Ø 30 µm, magnetic core coated with 6 % agarose 🔹MagStrep® Strep-Tactin® beads - Purification of biotinylated, Strep-tag®II and Twin-Strep-tag® fusion proteins, Ø 30 µm, magnetic core coated with 6 % agarose 🔹Strep-Tactin® Magnetic Microbeads - Multimerization of Strep-tag®II and Twin-Strep-tag® fusion proteins for cell isolation, Ø 1-3 µm, magnetic microspheres #biotech #biotechnology #proteinpurification #affinitychromatography