Papers by Rosa Alonso-Salces
Food Chemistry, Jun 1, 2022
According to the last report from the European Union (EU) Food Fraud Network, olive oil tops the ... more According to the last report from the European Union (EU) Food Fraud Network, olive oil tops the list of the most notified products. Current EU regulation states geographical origin as mandatory for virgin olive oils, even though an official analytical method is still lacking. Verifying the compliance of label-declared EU oils should be addressed with the highest priority level. Hence, the present work tackles this issue by developing a classification model (PLS-DA) based on the sesquiterpene hydrocarbon fingerprint of 400 samples obtained by HS-SPME-GC-MS to discriminate between EU and non-EU olive oils, obtaining an 89.6% of correct classification for the external validation (three iterations), with a sensitivity of 0.81 and a specificity of 0.95. Subsequently, multi-class discrimination models for EU and non-EU countries were developed and externally validated (with three different validation sets) with successful results (average of 92.2% of correct classification for EU and 96.0% for non-EU countries).
Spanish Journal of Agricultural Research, 2019
Chemistry & Biodiversity, Mar 20, 2017
Journal of the International Society of Antioxidants in Nutrition & Health, 2016
Polyphenols may play a role in plant response to biotic-stress and in resistance mechanisms. We a... more Polyphenols may play a role in plant response to biotic-stress and in resistance mechanisms. We aimed at testing the correlation between phenol concentration in apple and cultivar tolerance to rosy apple aphid (RAA), Dysaphis plantaginea, a key pest of apple on a full-sib family produced at SERIDA, derived of a crossing between the local cultivar ‘Meana’ and the resistant ‘Florina’. The analysis of flavan-3 ols, hydroxycinnamic acids, dyhidrochalcones and flavonols by UHPLC-DAD was carried out on the two parents and 154 descendants. Their resistance to RAA was also determined after aphid inoculation in greenhouse. A relationship between the concentration of three caffeoylquinic acid isomers and 4-p-coumaroylquinic acid and the resistance to RAA was found by pattern recognition techniques. This progeny was also genotyped using the Illumina Infinium Apple_20k SNP array. A number of QTLs on linkage group 8 (LG8) associated with hydroxycinnamic acids were found. The RAA resistance locus...
Spanish Journal of Agricultural Research, 2019
Aim of study: To evaluate the potential bactericidal activity of natural molecules against Paenib... more Aim of study: To evaluate the potential bactericidal activity of natural molecules against Paenibacillus larvae. Moreover, we investigated if molecules that exhibit antimicrobial activity were able to inhibit the proteolytic activity of the bacterium.Area of study: Isolates S1 and S2 were from Balcarce, Buenos Aires province, strain S3 from Rio Cuarto, Cordoba province, strain S4 from Concordia, Entre Rios province, strain S5 and S8 from Necochea, Buenos Aires, strain S6 and S7 from Mar del Plata, Buenos Aires, strain S9 from Modena, Italy and strain S10 from Emilia Reggio, Italy.Material and methods: Bacterial isolates identification was carried out by amplification of a specific 16S rRNA gene fragment of P. larvae using primers PL5 and PL4. Screening of the antimicrobial activity of thirteen molecules against four P. larvae isolates was conducted by the agar diffusion technique. The antimicrobial activity of selected molecules was evaluated by broth microdilution method.Main resul...
Journal of Food Protection, 2004
The levels of polycyclic aromatic hydrocarbons (PAHs) were determined by reversed-phase high-perf... more The levels of polycyclic aromatic hydrocarbons (PAHs) were determined by reversed-phase high-performance liquid chromatography with fluorescence detection in different fatty foods from a Spanish market. The average concentration of the sum of total PAHs in edible vegetable oils was below 25 ng/g, whereas the sum of heavy PAHs did not surpass 5 ng/g. Olive pomace oils obtained before the summer of 2001 were an exception because they were highly contaminated. The effects of different technological processes, such as bleaching, deodorization, and hydrogenation, on PAH concentration in edible oils have been studied. The PAH profiles, as well as the influence of cooking procedures, of other fatty foods (margarine, mayonnaise, and oils from canned fishes) have been examined.
Journal of mass spectrometry : JMS, Jan 22, 2017
Lettuce (Lactuca sativa) is one of the most popular leafy vegetables in the world and constitutes... more Lettuce (Lactuca sativa) is one of the most popular leafy vegetables in the world and constitutes a major dietary source of phenolic compounds with health promoting properties. In particular, the demand for green and red oak-leaf lettuces have considerably increased in the last years but few data on their polyphenol composition is available. Moreover, the utilization of analytical edge technology can provide new structural information and allow the identification of unknown polyphenols. In the present study the phenolic profiles of green and red oak-leaf lettuce cultivars were exhaustively characterized by ultrahigh performance liquid chromatography (UHPLC) coupled online to diode array detection (DAD), electrospray ionization (ESI) and quadrupole time-of-flight mass spectrometry (QToF/MS), using the MS(E) instrument acquisition mode for recording simultaneously exact masses of precursor and fragment ions. One hundred fifteen phenolic compounds were identified in the acidified hydro...
European Journal of Lipid Science and Technology, Apr 29, 2015
1H‐NMR spectral data and H and C isotope abundances of virgin olive oils (VOOs) and their unsapon... more 1H‐NMR spectral data and H and C isotope abundances of virgin olive oils (VOOs) and their unsaponifiable fractions were analyzed by pattern recognition techniques, such as principal component analysis (PCA) and partial‐least squares discriminant analysis (PLS‐DA). The aim was to develop chemical tools for the authentication of VOOs according to their geographical origin or protected designation of origin (PDO), as well as to detect the mislabeling of the provenance of VOOs, at the regional or national level, or the mislabeling of non‐PDO oils as PDO VOOs. The relationship between stable isotope abundances of the VOOs and their unsaponifiable fractions and the latitude of the VOO geographical origin was confirmed, but these criteria were not completely discriminant to differentiate VOOs according to their geographical origin. However, δ2H and/or δ13C data provided complementary geographical information to 1H‐NMR data in the PLS‐DA binary classification models afforded for VOOs from Greece, Spain, Italy, Izmir (Turkey), Crete (Greece), and the PDOs Riviera Ligure (Italy) and Huile d'olive d'Aix−en−Provence (France). 2H/1H and 13C/12C ratios of the unsaponifiable fractions of VOOs are reported here for the first time. The present approach for PDO Riviera Ligure VOOs, based on 1H‐NMR data and C isotope abundance of the bulk oil and its unsaponifiable fraction, outperformed the previously reported classification models. Moreover, the PLS‐DA models to authenticate VOOs from Greece and detect non‐Greek VOOs achieved over 93% of correct predictions.Practical applications: The research can be applied in the protection of consumers and honest producers and retailers, and provides potential tools for antifraud authorities and regulatory bodies, which face the challenge of detecting fraudulent practices that do not comply with EU regulations in the trade of VOOs, such as the mislabeling of VOOs produced in a certain geographical origin [Commission Implementing Regulation (EC) no 29/2012 and Commission Implementing Regulation (EC) no 1335/2013] and/or under specific EU quality schemes, named PDO or PGI [Council Regulation (EC) no 510/2006].1H‐NMR spectral data and H and C isotope abundances of virgin olive oils (VOOs) and their unsaponifiable fractions were analyzed by pattern recognition techniques, in order to develop chemical tools for the authentication of VOOs according to their geographical origin or protected designation of origin (PDO), as well as to detect the mislabeling of the provenance of VOOs, at the regional or national level, or the mislabeling of non‐PDO oils as PDO VOOs.
Academia Biology
The characterization of Argentinean honeys from the vegetation units (VUs) of the phytogeographic... more The characterization of Argentinean honeys from the vegetation units (VUs) of the phytogeographical provinces located in Catamarca and La Rioja was performed with the analysis used for honey quality control: sugar profile, free acidity, pH, electrical conductivity (EC), color and contents of moisture, ash, total soluble solids, and hydroxymethylfurfural (HMF). Honeys were authentic and traceable, and complied with the specifications of national and/or international standards, verifying their blossom origin, high quality, good maturity, and freshness. Honeys from VU-9 and VU-23 presented significantly distinctive physicochemical parameters, evidencing the influence of the flora and pedoclimatic conditions of these VUs. Honeys from VU-23 presented significantly higher contents of glucose and total reducing sugars (F + G), and lower EC, ash contents, fructose/glucose ratio, and concentrations of turanose and maltose than those from VU-9. Honeys from VU-23 in La Rioja also exhibited characteristic lighter colors and lower pH and ºBrix values and turanose amounts; and honeys from VU-9 in La Rioja exhibited highest pH values. Catamarca honeys were characterized by high free acidity and high amounts of turanose, regardless of the VUs. The contents of sugars and total soluble solids, moisture, pH, EC, and color of honeys from Catamarca and La Rioja are published here for the first time. This study contributes to the typification of honeys from these provinces, which will provide them an added value and allow them to access newer markets.
Academia Biology, Mar 10, 2023
The characterization of Argentinean honeys from the vegetation units (VUs) of the phytogeographic... more The characterization of Argentinean honeys from the vegetation units (VUs) of the phytogeographical provinces located in Catamarca and La Rioja was performed with the analysis used for honey quality control: sugar profile, free acidity, pH, electrical conductivity (EC), color and contents of moisture, ash, total soluble solids, and hydroxymethylfurfural (HMF). Honeys were authentic and traceable, and complied with the specifications of national and/or international standards, verifying their blossom origin, high quality, good maturity, and freshness. Honeys from VU-9 and VU-23 presented significantly distinctive physicochemical parameters, evidencing the influence of the flora and pedoclimatic conditions of these VUs. Honeys from VU-23 presented significantly higher contents of glucose and total reducing sugars (F + G), and lower EC, ash contents, fructose/glucose ratio, and concentrations of turanose and maltose than those from VU-9. Honeys from VU-23 in La Rioja also exhibited characteristic lighter colors and lower pH and ºBrix values and turanose amounts; and honeys from VU-9 in La Rioja exhibited highest pH values. Catamarca honeys were characterized by high free acidity and high amounts of turanose, regardless of the VUs. The contents of sugars and total soluble solids, moisture, pH, EC, and color of honeys from Catamarca and La Rioja are published here for the first time. This study contributes to the typification of honeys from these provinces, which will provide them an added value and allow them to access newer markets.
High-Resolution Mass Spectroscopy for Phytochemical Analysis, 2021
Journal of Apicultural Science, 2020
Apis mellifera populations are exposed to different biotic and abiotic stressors that affect bee ... more Apis mellifera populations are exposed to different biotic and abiotic stressors that affect bee survival. Paenibacillus larvae, the causative agent of American foulbrood, is one of the most important bacterial pathogens that affect bee health. In some countries, the use of antibiotics is the most common method for the prevention and treatment of P. larvae infected colonies, however this application can also increase the risk of occurrence of resistant strains. An ecological alternative is the use of vegetal extracts containing bioactive compounds, such as polyphenols. The aim of this study is to check the antimicrobial activity of phenolic extracts of apple pomace, from the cider industry, against P. larvae strains by the broth micro dilution method. In addition, the toxicity of the phenolic extracts on A. mellifera was verified using the complete exposure method. All extracts contain from 715.31 to 11348.16 μg gal/g DW, determined by Folin-Ciocalteu show antimicrobial activity aga...
The target of this paper is the study of anthocyanin and tannin extraction from red grape during ... more The target of this paper is the study of anthocyanin and tannin extraction from red grape during maceration, the formation of new pigments, and the evolution profiles of anthocyanin derivatives and tannins during the alcoholic and malolactic fermentations and aging in oak barrels and steel vats. 2 anthocyanins, 38 anthocyanin derivatives and 37 tannins were monitored. Formation of all classes of anthocyanin derivatives starts quickly after anthocyanin extraction from grape. Their maximum levels are reached a few days after apex of anthocyanins during maceration/alcoholic fermentation. Indirect condensation derivatives showed less stable behavior than that of the direct flavanol-anthocyanin products during fermentations and aging. Within pyranoanthocyanins, vitisin B had more instability than vitisin A. Extraction of tannins from grape occurs slower than that of anthocyanins. Major tannins are procyanidin homodimers with B bond (45% of total tannins after fermentations), followed by ...
Antioxidants
Antioxidant compounds with health benefits can be found in food processing residues, such as grap... more Antioxidant compounds with health benefits can be found in food processing residues, such as grape pomace. In this study, antioxidants were identified and quantified in an extract obtained from Graciano red grape pomace via a green process. The antioxidant activity of the extract was assessed by the DPPH and FRAP tests, and the phenolic content by the Folin–Ciocalteu test. Furthermore, nanotechnologies were employed to produce a safe and effective formulation that would exploit the antioxidant potential of the extract for skin applications. Anthocyanins, flavan-3-ols and flavanols were the main constituents of the grape pomace extract. Phospholipid vesicles, namely liposomes, were prepared and characterized. Cryo-TEM images showed that the extract-loaded liposomes were predominantly spherical/elongated, small, unilamellar vesicles. Light scattering results revealed that the liposomes were small (~100 nm), homogeneously dispersed, and stable during storage. The non-toxicity of the li...
International Journal of Science, Technology & Management
The honeys from the Phytogeographic Provinces (PP) of the south of Buenos Aires province (Argenti... more The honeys from the Phytogeographic Provinces (PP) of the south of Buenos Aires province (Argentina) were characterised by the sugar composition and physicochemical parameters used for honey quality control. A large set (n=329) of traceable raw honey samples obtained from beekeepers were analysed and met the specifications of the national and/or international standards for the evaluated parameters, which denoted their blossom origin and confirmed their authenticity, good maturity and freshness. The influence of flora and the pedoclimatic conditions of each phytogeographical region, as well as their beekeeping practices, on the physical and chemical properties of honey allowed its characterization. Thus, the honeys from the southeast of Buenos Aires province were characterized by higher contents of moisture, fructose, maltose, erlose, hydroxymethylfurfural (HMF) and ash, pH and electrical conductivity (EC) and slightly lighter colours; while southwest honeys showed higher free acidit...
Journal of Agricultural and Food Chemistry
Nanomaterials, 2022
Polyphenols are gaining increasing interest due to their beneficial properties to human health. G... more Polyphenols are gaining increasing interest due to their beneficial properties to human health. Grape pomace, the by-product of wine production, is a source of these bioactive compounds. An extract from Tempranillo grape pomace was obtained and characterized qualitatively and quantitatively. The major components found were anthocyanins, flavan-3-ols, and flavonols. To improve the bioavailability of these compounds, the extract was formulated in phospholipid vesicles, namely transfersomes. Spherical unilamellar vesicles around 100 nm each were obtained. The antioxidant activity of both the extract and the transfersomes was evaluated by using colorimetric assays (i.e., DPPH, FRAP, and Folin–Ciocalteu). The cells’ viability and the antioxidant activity were assessed in keratinocytes. The results showed that the extract and the transfersomes had no cytotoxic effects and exerted remarkable antioxidant activity, which was more evident in a vesicle formulation. These findings highlighted t...
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Papers by Rosa Alonso-Salces