Kaspaza-7

Kaspaza-7
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	1F1J, 1GQF, 1I4O, 1I51, 1K86, 1K88, 1KMC, 1SHJ, 1SHL, 2QL5, 2QL7, 2QL9, 2QLB, 2QLF, 2QLJ, 3EDR, 3H1P, 3IBC, 3IBF, 3R5K, 4FDL, 4FEA, 4HQ0, 4HQR, 4JB8, 4JJ8, 4JR1, 4JR2, 4LSZ, 4ZVR, 4ZVQ, 4ZVO, 4ZVU, 4ZVT, 4ZVS, 4ZVP, 5IC6
Identifikatori
Aliasi	CASP7
Vanjski ID-jevi	OMIM: 601761 MGI: 109383 HomoloGene: 11168 GeneCards: CASP7
Lokacija gena (čovjek)
Hrom.	Hromosom 10 (čovjek)
Kraj	113,730,907 bp
Lokacija gena (miš)
Hrom.	Hromosom 19 (miš)
Kraj	56,430,776 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• cysteine-type peptidase activity; • cysteine-type endopeptidase activity; • GO:0001948, GO:0016582 vezivanje za proteine; • hydrolase activity; • GO:0070122 peptidase activity; • cysteine-type endopeptidase activity involved in execution phase of apoptosis; • cysteine-type endopeptidase activity involved in apoptotic process;
Ćelijska komponenta	• citoplazma; • citosol; • jedro; • nukleoplazma;
Biološki proces	• Proteoliza; • cellular response to staurosporine; • execution phase of apoptosis; • GO:0097285 apoptoza;
	Izvori:Amigo / QuickGO
Ortolozi
	840
	12369
	ENSG00000165806
	ENSMUSG00000025076
	P55210
	P97864
NM_001227; NM_001267056; NM_001267057; NM_001267058; NM_033338;
	NM_033339; NM_033340; NM_001320911
	NM_007611
NP_001218; NP_001253985; NP_001253986; NP_001253987; NP_001307840;
	NP_203124; NP_203125; NP_203126
	NP_031637
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Kaspaza-7, apoptozno vezana cisteinska peptidaza, znana i kao CASP7, jest protein koji je kod ljudi kodiran genom CASP7 sa hromosoma 10.

Ortolozi CASP7^[5] identificirani su kod gotovo svih sisara za koje su dostupni potpuni podaci o genomu. Jedinstveni ortolozi su također prisutni u ptica, gušterova, Lissamphibia i Teleostea.

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 303 aminokiseline, a molekulska težina 34.277 Da.^[5]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MADDQGCIEE	QGVEDSANED	SVDAKPDRSS	FVPSLFSKKK	KNVTMRSIKT
TRDRVPTYQY	NMNFEKLGKC	IIINNKNFDK	VTGMGVRNGT	DKDAEALFKC
FRSLGFDVIV	YNDCSCAKMQ	DLLKKASEED	HTNAACFACI	LLSHGEENVI
YGKDGVTPIK	DLTAHFRGDR	CKTLLEKPKL	FFIQACRGTE	LDDGIQADSG
PINDTDANPR	YKIPVEADFL	FAYSTVPGYY	SWRSPGRGSW	FVQALCSILE
EHGKDLEIMQ	ILTRVNDRVA	RHFESQSDDP	HFHEKKQIPC	VVSMLTKELY
FSQ

Funkcija

Kaspaza-7 je član porodice proteina kaspaza (cistein [[asparaginska kiselinas|aspartat proteaza) i pokazalo se da je stražarski protein apoptoza. Sekvencijalna aktivacija kaspaza igra centralnu ulogu u fazi izvršenja ćelije apoptoza. Kaspaze postoje kao neaktivni proenzimi koji se podvrgavaju proteolitskoj obradi uzvodnim kaspazama (kapaza-8,9) na konzerviranim asparaginskim ostacima, kako bi se dobile dvije podjedinice, velika i mala, koje se dimeriziraju i formiraju aktivni enzim u obliku heterotetramer. Prekursor ove kaspaze cepaju kapazu-3, kapazu-10 i kapazu-9. Aktivira se na podražaje ćelijske smrti i inducira apoptozu. Alternativna prerada RNK rezultira u četiri varijante transkripta, kodirajući tri različite izoforme.^[6]

Interakcije

Pokazalo se da kaspaza 7 u interakciji sa:

Također pogledajte

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000165806 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000025076 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b "OrthoMaM phylogenetic marker: CASP7 coding sequence". Arhivirano s originala, 4. 3. 2016. Pristupljeno 20. 12. 2009.
^ "Entrez Gene: CASP7 caspase 7, apoptosis-related cysteine peptidase".
^ Guo Y, Srinivasula SM, Druilhe A, Fernandes-Alnemri T, Alnemri ES (Apr 2002). "Caspase-2 induces apoptosis by releasing proapoptotic proteins from mitochondria". J. Biol. Chem. 277 (16): 13430–7. doi:10.1074/jbc.M108029200. PMID 11832478.
^ Srinivasula SM, Ahmad M, Fernandes-Alnemri T, Litwack G, Alnemri ES (Dec 1996). "Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. Natl. Acad. Sci. U.S.A. 93 (25): 14486–91. Bibcode:1996PNAS...9314486S. doi:10.1073/pnas.93.25.14486. PMC 26159. PMID 8962078.
^ Tamm I, Wang Y, Sausville E, Scudiero DA, Vigna N, Oltersdorf T, Reed JC (Dec 1998). "IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs". Cancer Res. 58 (23): 5315–20. PMID 9850056.
^ Shin S, Sung BJ, Cho YS, Kim HJ, Ha NC, Hwang JI, Chung CW, Jung YK, Oh BH (Jan 2001). "An anti-apoptotic protein human survivin is a direct inhibitor of caspase-3 and -7". Biochemistry. 40 (4): 1117–23. doi:10.1021/bi001603q. PMID 11170436.
^ Riedl SJ, Renatus M, Schwarzenbacher R, Zhou Q, Sun C, Fesik SW, Liddington RC, Salvesen GS (Mar 2001). "Structural basis for the inhibition of caspase-3 by XIAP". Cell. 104 (5): 791–800. doi:10.1016/S0092-8674(01)00274-4. PMID 11257232. S2CID 17915093.
^ Roy N, Deveraux QL, Takahashi R, Salvesen GS, Reed JC (Dec 1997). "The c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases". EMBO J. 16 (23): 6914–25. doi:10.1093/emboj/16.23.6914. PMC 1170295. PMID 9384571.
^ Deveraux QL, Takahashi R, Salvesen GS, Reed JC (Jul 1997). "X-linked IAP is a direct inhibitor of cell-death proteases". Nature. 388 (6639): 300–4. Bibcode:1997Natur.388..300D. doi:10.1038/40901. PMID 9230442. S2CID 4395885.
^ Suzuki Y, Nakabayashi Y, Nakata K, Reed JC, Takahashi R (Jul 2001). "X-linked inhibitor of apoptosis protein (XIAP) inhibits caspase-3 and -7 in distinct modes". J. Biol. Chem. 276 (29): 27058–63. doi:10.1074/jbc.M102415200. PMID 11359776.

Dopunska literatura

Cohen GM (1997). "Caspases: the executioners of apoptosis". Biochem. J. 326 (Pt 1): 1–16. doi:10.1042/bj3260001. PMC 1218630. PMID 9337844.

Vanjski linkovi

The MEROPS online database for peptidases and their inhibitors: C14.004^{[mrtav link]}
P55210

Portal Biologija

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000165806 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000025076 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[OrthoMaM-5] "OrthoMaM phylogenetic marker: CASP7 coding sequence". Arhivirano s originala, 4. 3. 2016. Pristupljeno 20. 12. 2009.

[6] "Entrez Gene: CASP7 caspase 7, apoptosis-related cysteine peptidase".

[pmid11832478-7] Guo Y, Srinivasula SM, Druilhe A, Fernandes-Alnemri T, Alnemri ES (Apr 2002). "Caspase-2 induces apoptosis by releasing proapoptotic proteins from mitochondria". J. Biol. Chem. 277 (16): 13430–7. doi:10.1074/jbc.M108029200. PMID 11832478.

[pmid8962078-8] Srinivasula SM, Ahmad M, Fernandes-Alnemri T, Litwack G, Alnemri ES (Dec 1996). "Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. Natl. Acad. Sci. U.S.A. 93 (25): 14486–91. Bibcode:1996PNAS...9314486S. doi:10.1073/pnas.93.25.14486. PMC 26159. PMID 8962078.

[pmid9850056-9] Tamm I, Wang Y, Sausville E, Scudiero DA, Vigna N, Oltersdorf T, Reed JC (Dec 1998). "IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs". Cancer Res. 58 (23): 5315–20. PMID 9850056.

[pmid11170436-10] Shin S, Sung BJ, Cho YS, Kim HJ, Ha NC, Hwang JI, Chung CW, Jung YK, Oh BH (Jan 2001). "An anti-apoptotic protein human survivin is a direct inhibitor of caspase-3 and -7". Biochemistry. 40 (4): 1117–23. doi:10.1021/bi001603q. PMID 11170436.

[pmid11257232-11] Riedl SJ, Renatus M, Schwarzenbacher R, Zhou Q, Sun C, Fesik SW, Liddington RC, Salvesen GS (Mar 2001). "Structural basis for the inhibition of caspase-3 by XIAP". Cell. 104 (5): 791–800. doi:10.1016/S0092-8674(01)00274-4. PMID 11257232. S2CID 17915093.

[pmid9384571-12] Roy N, Deveraux QL, Takahashi R, Salvesen GS, Reed JC (Dec 1997). "The c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases". EMBO J. 16 (23): 6914–25. doi:10.1093/emboj/16.23.6914. PMC 1170295. PMID 9384571.

[pmid9230442-13] Deveraux QL, Takahashi R, Salvesen GS, Reed JC (Jul 1997). "X-linked IAP is a direct inhibitor of cell-death proteases". Nature. 388 (6639): 300–4. Bibcode:1997Natur.388..300D. doi:10.1038/40901. PMID 9230442. S2CID 4395885.

[pmid11359776-14] Suzuki Y, Nakabayashi Y, Nakata K, Reed JC, Takahashi R (Jul 2001). "X-linked inhibitor of apoptosis protein (XIAP) inhibits caspase-3 and -7 in distinct modes". J. Biol. Chem. 276 (29): 27058–63. doi:10.1074/jbc.M102415200. PMID 11359776.

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p r u Proteaza: Cisteinska proteaza (EC 3.4.22)
Kaspaza	Kaspaza-1 Kaspaza-2 Kaspaza-3 Kaspaza-4 Kaspaza-5 Kaspaza-6 Kaspaza-7 Kaspaza-8 Kaspaza-9 Kaspaza-10 Kaspaza-12 Kaspaza-13 Kaspaza-14
Izvedene iz plodova	Papain Fikain Bromelain Aktinidain
Kalpain	CAPN1 CAPN2 CAPN3 CAPN5 CAPN6 CAPN7 CAPN8 CAPN9 CAPN10 CAPN11 CAPN12 CAPN13 CAPN14 CAPNS1 CAPNS2
Katepsin	B C F H K L1/L2 O S W Z
Ostale	Klostripain Kancerski prokoagulant Separaza Autofagin Kruzipain 3C-lika proteaza

p r u Enzimi
Teme	Aktivno mjesto Alosterna regulacija Difuzijski limitirani enzim EC broj Enzimska kataliza Inhibicija enzimskih reakcija Kinetika enzima Koenzim Kooperativnost Lineweaver–Burkov dijagram Michaelis–Mentenova kinetika Mjesto vezanja Spisak enzima
Tipovi	EC1 Oksidoreduktaze/spisak EC2 Transferaze/spisak EC3 Hidrolaze/spisak EC4 Lijaze/spisak EC5 Izomeraze/spisak EC6 Ligaze/spisak