Histone deacetylase 6 (HDAC6) is a member of class IIb HDAC family. HDAC6 exists predominantly in the cytoplasm and deacetylates mainly non-histone proteins in the cytoplasm. Via its deacetylase and ubiquitin binding domains, HDAC6 regulates microtubules, cytoskeleton, intracellular trafficking, and cellular responses to stress. HDAC6 plays a central role in physiology and pathobiology in various organs and tissues. Herein we describe the methods for analysis of expression and function of HDAC6 in diverse physiological and pathological contexts.
Keywords: Acetylation; Chaperone; Histone deacetylase 6; Stress; Ubiquitin.