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Search Results (151)

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22 pages, 9464 KiB  
Article
Elucidating the Electrochemical Corrosion of a Water Pump Impeller in an Industrial Cooling System with Zero Liquid Discharge
by Mina Mousavi Jarrahi, Ehsan Khajavian, Amir Hossein Noorbakhsh Nezhad, Ehsan Mohammadi Zahrani and Akram Alfantazi
Water 2025, 17(2), 173; https://doi.org/10.3390/w17020173 - 10 Jan 2025
Viewed by 596
Abstract
The electrochemical corrosion of a single-suction centrifugal water pump impeller made of gray cast iron operating at 85 °C was investigated in two industrial water media, i.e., groundwater extracted from a borehole and treated wastewater. Open circuit potential (OCP) measurement plus potentiodynamic polarization [...] Read more.
The electrochemical corrosion of a single-suction centrifugal water pump impeller made of gray cast iron operating at 85 °C was investigated in two industrial water media, i.e., groundwater extracted from a borehole and treated wastewater. Open circuit potential (OCP) measurement plus potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) techniques elucidated the electrochemical corrosion performance and inductively coupled plasma-optical emission spectroscopy (ICP-OES) characterized the water samples. The retired and brand-new impellers were studied using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and visual and metallographic examinations. Impeller trailing edges were vulnerable to corrosion damage due to increased total fluid pressure, velocity, and temperature. The groundwater was more contaminated with Ca, Mg, Na, Si, and S elements and possessed higher conductivity, pH, and suspended solids than the treated wastewater. The impeller was more susceptible to graphitic corrosion in the groundwater due to emerging microgalvanic cells. A kinetic control electrochemical mechanism was elucidated as the corrosion rate-controlling step in the wastewater. A mixed kinetic and diffusion control mechanism was predominant in the groundwater because a short Warburg impedance element emerged. This study showcased the significance of integrated industrial water management and treatment strategies to protect pumps’ integrity and uptime in critical industrial units implementing a zero-liquid discharge program. Full article
(This article belongs to the Special Issue Water Engineering Safety and Management)
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18 pages, 5101 KiB  
Article
Life Cycle Assessment of Biochar from Residual Lignocellulosic Biomass Using Kon-Tiki Kilns: Applications in Soil Amendment and Wastewater Filtration
by Roxanna Pamela Ramírez López, Diana Cabañas Vargas, Erick Alberto Aguilera-Cauich and Julio César Sacramento Rivero
Viewed by 599
Abstract
Producing biochar from residual biomass is an opportunity for health, environmental, and economic benefits to farmers in small traditional parcels, which are widespread in Latin America. This study presents a life cycle assessment of biochar in two circular economy scenarios: soil amendment and [...] Read more.
Producing biochar from residual biomass is an opportunity for health, environmental, and economic benefits to farmers in small traditional parcels, which are widespread in Latin America. This study presents a life cycle assessment of biochar in two circular economy scenarios: soil amendment and wastewater filtration. Seven mid-point environmental impact categories were assessed using the CML-IA method: acidification (AP), abiotic depletion (ADP), fossil fuels depletion (ADP-FF), eutrophication (EP), global warming (GWP), human toxicity (HTP), and smog formation (POCP). The soil amendment scenario showed lower impacts per tonne of biochar in all categories, especially for GWP (−801.3 kg CO2eq) and ADP-FF (−374.3 MJ), compared to the filtration scenario (−123.54 kg CO2eq and 827.85 MJ). Negative GWP values reflect reduced emissions from avoided fertilizers and carbon sequestration. However, POCP and HTP increased due to air emissions (CH4, NOx, NMVOC, and PM10) from the kiln. In both scenarios, biochar production contributed to 40–90% of the total impacts. Indirect emissions from electricity used for water pumping were identified as a hotspot in the filtration scenario. Full article
(This article belongs to the Special Issue Sustainability of the Circular Economy)
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17 pages, 1832 KiB  
Article
The First Fifth-Generation District Heating and Cooling System in Kazakhstan: Planning and Design
by Stanislav Chicherin, Yana Zhuikova, Tatyana Pyanykh, Andrey Zhuikov, Galym Baidyussenov and Saule Abildinova
Energies 2024, 17(23), 6169; https://doi.org/10.3390/en17236169 - 6 Dec 2024
Viewed by 704
Abstract
This paper focuses on the potential for integrating Geographic Information System (GIS) software into Fifth-Generation District Heating and Cooling (5GDHC) systems to promote efficient and sustainable energy management, particularly in Kazakhstan. By reviewing the key literature, we identify three main areas where GIS [...] Read more.
This paper focuses on the potential for integrating Geographic Information System (GIS) software into Fifth-Generation District Heating and Cooling (5GDHC) systems to promote efficient and sustainable energy management, particularly in Kazakhstan. By reviewing the key literature, we identify three main areas where GIS software enhances the planning of 5GDHC systems: decision-making in the context of energy market regulations, operational data utilization, and modeling/simulations for technical design. The technical design emphasizes the role of heat pumps, ultra-low temperature district heating (ULTDH) networks, and end-user buildings. Previous research has explored various methodologies for integrating network and demand-side strategies, developing sustainable district heating and cooling (DHC) systems, and mapping urban areas suitable for DHC deployment. However, none has presented an open-source model incorporating GIS-based decision-making in designing 5GDHC systems. This study, for the first time, addresses this gap through a case study conducted in the Northern Industrial Zone of Karaganda, Kazakhstan, demonstrating how GIS-enhanced modeling can be effectively applied in a developing, industry-driven economy. We outline the mathematical framework for comparing existing simulation tools and developing a custom model suited to the region’s needs. Additionally, we discuss validation and calibration methods, which remain underexplored in the current literature. The proposed model incorporates waste heat recovery from local sources, including nearby wastewater treatment plants, showcasing a sustainable energy solution for the industrial park. The results indicate that a well-structured 5GDHC system, supported by GIS tools, can markedly enhance energy efficiency and sustainability, presenting a scalable and adaptable approach for other regions in Kazakhstan and beyond. Full article
(This article belongs to the Section J: Thermal Management)
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19 pages, 11138 KiB  
Article
An Environmentally Friendly Superhydrophobic Wood Sponge with Photo/Electrothermal Effects Prepared from Natural Wood for All-Weather High-Viscosity Oil–Water Separation
by Kenan Yang, Sainan Wang, Bin Du and Shisheng Zhou
Polymers 2024, 16(23), 3256; https://doi.org/10.3390/polym16233256 - 23 Nov 2024
Viewed by 511
Abstract
Rapid industrial development has led to increased crude oil extraction and oily wastewater discharge. Achieving oil–water separation and marine oil adsorption in a cost-effective, efficient, and environmentally friendly manner remains a global challenge. In this work, natural wood was chemically treated to prepare [...] Read more.
Rapid industrial development has led to increased crude oil extraction and oily wastewater discharge. Achieving oil–water separation and marine oil adsorption in a cost-effective, efficient, and environmentally friendly manner remains a global challenge. In this work, natural wood was chemically treated to prepare a degradable and environmentally friendly wood sponge structure. In situ polymerization and spraying methods were used to produce an environmentally friendly oil–water separation sponge with superhydrophobic and superoleophilic properties (Fe3O4@P-P@WS). Fe3O4@P-P@WS had excellent superhydrophobicity (WCA = 154.2°) and self-cleaning properties. Additionally, Fe3O4@P-P@WS could convert solar and electrical energy into thermal energy, reaching a surface temperature of 74 °C under sunlight irradiation with an intensity of 1.0 kW m−2. When a voltage of 9 V was applied, the surface temperature reached 120.5 °C. Moreover, under the suction of a vacuum pump or the action of gravity, the continuous separation of highly fluid oil substances was achieved. The designed Fe3O4@P-P@WS offers advantages such as easily obtained raw materials, energy efficiency, simple preparation, and the ability to solve secondary pollution issues, providing a new technology for cleaning organic matter in industrial wastewater discharge and for round-the-clock cleaning of high-viscosity crude oil leaked during offshore oil exploitation. Full article
(This article belongs to the Section Polymer Applications)
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27 pages, 13333 KiB  
Article
Numerical Prediction of Solid Particle Erosion in Jet Pumps Based on a Calibrated Model
by Xuanchen Wan, Mengxue Dong, Maosen Xu, Chuanhao Fan, Jiegang Mou and Shuai Han
Energies 2024, 17(22), 5720; https://doi.org/10.3390/en17225720 - 15 Nov 2024
Viewed by 529
Abstract
Jet pumps are widely used in petrochemical processes, nuclear cooling, and wastewater treatment due to their simple structure, high reliability, and stable performance under extreme conditions. However, when transporting solid-laden two-phase flows, they face severe erosion problems, leading to reduced efficiency, malfunctions, or [...] Read more.
Jet pumps are widely used in petrochemical processes, nuclear cooling, and wastewater treatment due to their simple structure, high reliability, and stable performance under extreme conditions. However, when transporting solid-laden two-phase flows, they face severe erosion problems, leading to reduced efficiency, malfunctions, or even failure. Therefore, optimizing jet pump performance and extending its service life is crucial. In this study, an experimental platform was established to conduct experiments on wall erosion in jet pumps. The CFD-DEM method was used to simulate the solid–liquid two-phase flow in the jet pump, comparing six erosion models for predicting erosion rates. The Grey Wolf Optimization algorithm was applied to calibrate model coefficients. The results indicate that the Neilson erosion model shows the best consistency with the experimental results. The inlet flow rate significantly influenced the erosion rates, while the flow rate ratio had a smaller effect. The particle concentration exhibited a nonlinear relationship with erosion, with diminishing impact beyond a certain threshold. As the factors varied, the erosion distribution tended to be uniform, but high erosion areas remained locally concentrated, indicating intensified localized erosion. Full article
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19 pages, 19600 KiB  
Article
Impacts of Remedial Techniques on Contamination Transport in Groundwater
by Walid M. A. Khalifa, Belkacem Achour, Tayyab Butt, Cyrus Raza Mirza, Heba Salah and Sherif M. El-Didy
Water 2024, 16(22), 3277; https://doi.org/10.3390/w16223277 - 14 Nov 2024
Viewed by 562
Abstract
The significance of groundwater is largely shaped by the quality of wastewater from industrial, agricultural, and municipal sources. Understanding the controlling factors is essential to prevent the spread of contamination in groundwater. These factors could be divided into physical defenses, such as grouting [...] Read more.
The significance of groundwater is largely shaped by the quality of wastewater from industrial, agricultural, and municipal sources. Understanding the controlling factors is essential to prevent the spread of contamination in groundwater. These factors could be divided into physical defenses, such as grouting and slurry walls, and hydrodynamic factors, such as injection and pumping wells. In this study, the groundwater transport model (MT3D) and the flow model (MODFLOW) were used to simulate four scenarios for groundwater protection. The first and second scenarios involve grouting and constructing slurry walls to change their depth, permeability, and thickness. The third and fourth scenarios involve injection and pumping wells changing the rate of flow, screen length, and the number of wells. The results show that increasing the thickness of the grouted soil and increasing the grouting depth help to control the level of contamination. Furthermore, multi-slurry walls upstream or downstream of the contamination source are sufficient for preventing the spread of contaminants. The results also reveal that rising rates of injection or pumping wells allow for minimal contamination propagation. The growing number of wells provided greater control over the injection rather than pumping wells. The variation in the screen length of pumping wells is effective for preventing the propagation of contamination. Full article
(This article belongs to the Section Hydrogeology)
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30 pages, 12807 KiB  
Article
Model-Based Assessment of Energy Efficiency in Industrial Pump Systems: A Case Study Approach
by Henrik Lavrič, Klemen Drobnič and Rastko Fišer
Appl. Sci. 2024, 14(22), 10430; https://doi.org/10.3390/app142210430 - 13 Nov 2024
Viewed by 1000
Abstract
Outdated, oversized variable speed pump drives (VSDPs) in industry lead to sub-optimal energy efficiency and considerable energy losses. This paper proposes methods to develop 2D efficiency maps for motors, converters, and pumps using polynomial surface fitting, which enables efficiency evaluation in a wide [...] Read more.
Outdated, oversized variable speed pump drives (VSDPs) in industry lead to sub-optimal energy efficiency and considerable energy losses. This paper proposes methods to develop 2D efficiency maps for motors, converters, and pumps using polynomial surface fitting, which enables efficiency evaluation in a wide operating range. The method was applied to an oversized VSDP in an industrial chilled water supply system, comparing the original system with five alternative VSDP combinations with high-efficiency motors and pumps. The five VSDP variants demonstrated average energy savings of around 30%, with the synchronous reluctance motor (SRM) configurations outperforming the induction motor (IM) configurations by up to 7 percentage points, particularly at low loads. The high-efficiency SRM-based 252-IE5 variant delivered the best overall energy performance, highlighting the benefits of optimised system sizing and motor selection for energy savings. The proposed method can be used in both industrial and residential applications and offers great advantages in process systems that require variable flow and pressure of water or other fluids during operation, such as HVAC, water supply and wastewater treatment, district heating, etc. The development of a VSDP drive with efficient energy optimisation is an interdisciplinary problem of mechanical and electrical engineering, and without the interaction of engineers from both fields the result will not be optimal. We try to present our method so that it can be a reliable tool for mechanical, electrical, and other engineers or researchers to assist them in finding possible energy savings, performing energy audits, and selecting the most suitable components when modernising existing or developing new systems. Full article
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24 pages, 6433 KiB  
Article
Research on Hybrid Heating System in Cold Oilfield Regions
by Meng Xu, Zhiyang Xu, Xinxin Wei, Gaoxiang Zhang and Changyu Liu
Clean Technol. 2024, 6(4), 1480-1503; https://doi.org/10.3390/cleantechnol6040071 - 2 Nov 2024
Viewed by 989
Abstract
Efficient and clean treatment of wastewater and energy recovery and utilization are important links to realize low-carbon development of oilfields. Therefore, this paper innovatively proposes a multi-energy complementary co-production heating system which fully and efficiently utilizes solar energy resources, oilfield waste heat resources, [...] Read more.
Efficient and clean treatment of wastewater and energy recovery and utilization are important links to realize low-carbon development of oilfields. Therefore, this paper innovatively proposes a multi-energy complementary co-production heating system which fully and efficiently utilizes solar energy resources, oilfield waste heat resources, and biomass resources. At the same time, a typical dormitory building in the oil region was selected as the research object, the system equipment selection was calculated according to the relevant design specifications. On this basis, the simulation system model is established, and the evaluation index and operation control strategy suitable for the system are proposed. The energy utilization rate of the system and the economic, energy-saving, and environmental benefits of the system are simulated. The results show that, under the simulated conditions of two typical days and a heating season, the main heat load of the system is borne by the sewage source heat pump, the energy efficiency is relatively low in the cold period, and the energy-saving characteristics are not obvious. With the increase in heating temperature and anaerobic reactor volume, the energy consumption of the system also increases, and the energy efficiency ratio of each subsystem and the comprehensive energy efficiency ratio of the system gradually decrease. In addition, although the initial investment in cogeneration heating systems is high, the operating costs and environmental benefits are huge. Under the condition of maintaining 35 °C, the anaerobic reactor in the system can reduce carbon emissions by 12.15 t per year, reduce sulfur dioxide emissions by 98.4 kg, reduce dust emissions by 49.2 kg, and treat up to 2700 t of sewage per year, which has broad application prospects. Full article
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11 pages, 2502 KiB  
Article
Distribution of Microorganisms and Antibiotic Resistance Genes in Production Wastewater During Pumped Storage Power Station Construction
by Qiang Wu, Xiaoxiao Ma, Chunliang Wang, Kai Yan, Chao Liu, Fan Liu, Bing Li and Yong Qiu
Water 2024, 16(21), 3058; https://doi.org/10.3390/w16213058 - 25 Oct 2024
Viewed by 821
Abstract
The construction period of pumped storage power stations (PSPS) generates amounts of production wastewater, which may contain pathogenic bacteria and antibiotic resistance genes (ARGs) in these bacteria, potentially posing environmental and health risks. This study used the metagenome approach to analyze the distribution [...] Read more.
The construction period of pumped storage power stations (PSPS) generates amounts of production wastewater, which may contain pathogenic bacteria and antibiotic resistance genes (ARGs) in these bacteria, potentially posing environmental and health risks. This study used the metagenome approach to analyze the distribution of microorganisms, ARGs and their correlation with water quality indicators in wastewater collected from two typical PSPSs. Coagulation system wastewater exhibits strong alkalinity (11.88), and aggregate system wastewater has high suspended solids (SS, 8 × 104 mg/L), resulting in lower richness and diversity of bacterial communities. Serpentinimonas, a kind of alkaliphilic bacteria, had the highest relative abundance (48.58–99.7%). The ARG subtypes obtained conferred wastewater resistance to tetracycline, macrolide, fluoroquinolone and so on, but wastewater treatment has limited removal effect on ARGs. The results indicate that resistant bacteria and resistance genes can still be present and distributed under highly alkaline conditions, and the removal efficiency of ARGs by wastewater treatment in PSPS is limited. Attention should be given to the environmental and health risks posed by production wastewater, thereby providing a theoretical basis for the sustainable development of the PSPS industry. Full article
(This article belongs to the Section Water and One Health)
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35 pages, 17147 KiB  
Article
Utilizing Wastewater Tunnels as Thermal Reservoirs for Heat Pumps in Smart Cities
by Fredrik Skaug Fadnes and Mohsen Assadi
Energies 2024, 17(19), 4832; https://doi.org/10.3390/en17194832 - 26 Sep 2024
Cited by 1 | Viewed by 749
Abstract
The performance of heat pump systems for heating and cooling heavily relies on the thermal conditions of their reservoirs. This study introduces a novel thermal reservoir, detailing a 2017 project where the Municipality of Stavanger installed a heat exchanger system on the wall [...] Read more.
The performance of heat pump systems for heating and cooling heavily relies on the thermal conditions of their reservoirs. This study introduces a novel thermal reservoir, detailing a 2017 project where the Municipality of Stavanger installed a heat exchanger system on the wall of a main wastewater tunnel beneath the city center. It provides a comprehensive account of the system’s design, installation, and performance, and presents an Artificial Neural Network (ANN) model that predicts heat pump capacity, electricity consumption, and outlet temperature across seasonal variations in wastewater temperatures. By integrating domain knowledge with the ANN, this study demonstrates the model’s capability to detect anomalies in heat pump operations effectively. The network also confirms the consistent performance of the heat exchangers from 2020 to 2024, indicating minimal fouling impacts. This study establishes wastewater heat exchangers as a safe, effective, and virtually maintenance-free solution for heat extraction and rejection. Full article
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4 pages, 784 KiB  
Proceeding Paper
Optimal Sewer Network Design Including Pumping Stations
by Juan Saldarriaga, Juana Herrán, María A. González, Yesid Coy and Pedro L. Iglesias-Rey
Eng. Proc. 2024, 69(1), 16; https://doi.org/10.3390/engproc2024069016 - 30 Aug 2024
Viewed by 509
Abstract
In urban areas with a flat terrain, pumping stations must be included to elevate wastewater and avoid extreme excavation depths. These systems are characterized by high operational costs due to the pump’s power consumption. The present work presents a methodology for the optimal [...] Read more.
In urban areas with a flat terrain, pumping stations must be included to elevate wastewater and avoid extreme excavation depths. These systems are characterized by high operational costs due to the pump’s power consumption. The present work presents a methodology for the optimal design of sewer networks including pumping stations, whose objective function is to minimize the construction and operation costs of the system. The methodology was tested on three sewer benchmark networks using two cost functions proposed in the literature. In all the sewer benchmarks, the cost achieved in the present work was compared with the best costs reported in the literature. Full article
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13 pages, 580 KiB  
Article
The Quest for Industrially and Environmentally Efficient Nanobubble Engineering: Electric-Field versus Mechanical Generation Approaches
by Niall J. English
Appl. Sci. 2024, 14(17), 7636; https://doi.org/10.3390/app14177636 - 29 Aug 2024
Cited by 1 | Viewed by 1200
Abstract
Nanobubbles (NBs) are gaseous domains at the nanoscale that can exist in bulk liquid or on solid surfaces. They are noteworthy for their high potential for real-world applications and their long (meta)stability. “Platform-wide” applications abound in medicine, wastewater treatment, hetero-coagulation, boundary-slip control in [...] Read more.
Nanobubbles (NBs) are gaseous domains at the nanoscale that can exist in bulk liquid or on solid surfaces. They are noteworthy for their high potential for real-world applications and their long (meta)stability. “Platform-wide” applications abound in medicine, wastewater treatment, hetero-coagulation, boundary-slip control in microfluidics, and nanoscopic cleaning. Here, we compare and contrast the industrial NB-generation performance of various types of commercial NB generators in both water-flow and submerged-in-water settings—in essence, comparing electric-field NB-generation approaches versus mechanical ones—finding that the former embodiments are superior from a variety of perspectives. It was found that the electric-field approach for NB generation surpasses traditional mechanical approaches for clean-water NB generation, especially when considering the energy running cost. In particular, more passive electric-field approaches are very operationally attractive for NB generation, where water and gas flow can be handled at little to no cost to the end operator, and/or submersible NB generators can be deployed, allowing for the use of photovoltaic approaches (with backup batteries for night-time and “low-sun” scenarios and air-/CO2-pumping paraphernalia). Full article
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13 pages, 276 KiB  
Article
Green Transformation of Mining towards Energy Self-Sufficiency in a Circular Economy—A Case Study
by Małgorzata Magdziarczyk, Andrzej Chmiela, Weijian Su and Adam Smolinski
Energies 2024, 17(15), 3771; https://doi.org/10.3390/en17153771 - 31 Jul 2024
Cited by 1 | Viewed by 963
Abstract
This article presents the concept of green transformation of the coal mining sector. Pump stations that belong to Spółka Restrukturyzacji Kopalń S.A. (SRK S.A., Bytom, Poland) pump out approximately 100 million m3 of mine water annually. These pump stations protect neighboring mines [...] Read more.
This article presents the concept of green transformation of the coal mining sector. Pump stations that belong to Spółka Restrukturyzacji Kopalń S.A. (SRK S.A., Bytom, Poland) pump out approximately 100 million m3 of mine water annually. These pump stations protect neighboring mines and lower-lying areas from flooding and protect subsurface aquifers from contamination. The largest cost component of maintaining a pumping station is the expenditure for purchasing electricity. Investment towards renewable energy sources will reduce the environmental footprint of pumping station operation by reducing greenhouse gas emissions. The concept of liquidation of an exemplary mining site in the context of a circular economy by proposing the development/revitalization of a coal mine site is presented. This concept involves the construction of a complex consisting of photovoltaic farms combined with efficient energy storage in the form of green hydrogen produced by water electrolysis. For this purpose, the potential of liquidated mining sites will be utilized, including the use of pumped mine wastewater. This article is conceptual. In order to reach the stated objective, a body of literature and legal regulations was analyzed, and an empirical study was conducted. Various scenarios for the operation of mine pumping stations have been proposed. The options presented provide full or nearly full energy self-sufficiency of the proposed pumping station operation concept. The effect of applying any option for upgrading the pumping station could result in the creation of jobs that are alternatives to mining jobs and a guarantee of efficient asset management. Full article
13 pages, 2902 KiB  
Article
Effect of Stepwise Exposure to High-Level Erythromycin on Anaerobic Digestion
by Yanxiang Zhang, Chunxing Li, Xinyu Zhu and Irini Angelidaki
Molecules 2024, 29(15), 3489; https://doi.org/10.3390/molecules29153489 - 25 Jul 2024
Viewed by 1092
Abstract
High-level erythromycin (ERY) fermentation wastewater will pose serious threats to lake environments. Anaerobic digestion (AD) has advantages in treating high-level antibiotic wastewater. However, the fate of antibiotic resistance genes (ARGs) and microbial communities in AD after stepwise exposure to high-level ERY remains unclear. [...] Read more.
High-level erythromycin (ERY) fermentation wastewater will pose serious threats to lake environments. Anaerobic digestion (AD) has advantages in treating high-level antibiotic wastewater. However, the fate of antibiotic resistance genes (ARGs) and microbial communities in AD after stepwise exposure to high-level ERY remains unclear. In this study, an AD reactor was first exposed to 0, 5, 10, 50, 100 and 200 mg/L ERY and then re-exposed to 0, 50, 200 and 500 mg/L ERY to investigate the effect of ERY on AD. The results show that AD could adapt to the presence of high-level ERY (500 mg/L) and could maintain efficient CH4 production after domestication with low-level ERY (50 mg/L). The AD process could achieve higher removal of ERY (>94%), regardless of the initial ERY concentration. ErmB and mefA, conferring resistance through target alteration and efflux pumps, respectively, were dominant in the AD process. The first exposure to ERY stimulated an increase in the total ARG abundance, while the AD process seemed to discourage ARG maintenance following re-exposure to ERY. ERY inhibited the process of acetoclastic methanogenesis, but strengthened the process of hydrogenotrophic methanogenesis. This work provides useful information for treating high-level ERY fermentation wastewater by the AD process. Full article
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18 pages, 2135 KiB  
Article
SARS-CoV-2 RNA Detection in Wastewater and Its Effective Correlation with Clinical Data during the Outbreak of COVID-19 in Salamanca
by Ángel Emilio Martínez de Alba, María Eugenia Morán-Diez, Juan Carlos García-Prieto, Juan García-Bernalt Diego, Pedro Fernández-Soto, Esteban Serrano León, Víctor Monsalvo, Marta Casao, María Belén Rubio, Rosa Hermosa, Antonio Muro, Manuel García-Roig and Enrique Monte
Int. J. Mol. Sci. 2024, 25(15), 8071; https://doi.org/10.3390/ijms25158071 - 24 Jul 2024
Viewed by 1368
Abstract
Wastewater treatment plants (WWTPs) are the final stage of the anthropogenic water cycle where a wide range of chemical and biological markers of human activity can be found. In COVID-19 disease contexts, wastewater surveillance has been used to infer community trends based on [...] Read more.
Wastewater treatment plants (WWTPs) are the final stage of the anthropogenic water cycle where a wide range of chemical and biological markers of human activity can be found. In COVID-19 disease contexts, wastewater surveillance has been used to infer community trends based on viral abundance and SARS-CoV-2 RNA variant composition, which has served to anticipate and establish appropriate protocols to prevent potential viral outbreaks. Numerous studies worldwide have provided reliable and robust tools to detect and quantify SARS-CoV-2 RNA in wastewater, although due to the high dilution and degradation rate of the viral RNA in such samples, the detection limit of the pathogen has been a bottleneck for the proposed protocols so far. The current work provides a comprehensive and systematic study of the different parameters that may affect the detection of SARS-CoV-2 RNA in wastewater and hinder its quantification. The results obtained using synthetic viral RNA as a template allow us to consider that 10 genome copies per µL is the minimum RNA concentration that provides reliable and consistent values for the quantification of SARS-CoV-2 RNA. RT-qPCR analysis of wastewater samples collected at the WWTP in Salamanca (western Spain) and at six pumping stations in the city showed that below this threshold, positive results must be confirmed by sequencing to identify the specific viral sequence. This allowed us to find correlations between the SARS-CoV-2 RNA levels found in wastewater and the COVID-19 clinical data reported by health authorities. The close match between environmental and clinical data from the Salamanca case study has been confirmed by similar experimental approaches in four other cities in the same region. The present methodological approach reinforces the usefulness of wastewater-based epidemiology (WBE) studies in the face of future pandemic outbreaks. Full article
(This article belongs to the Section Molecular Microbiology)
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